PENGARUH AIR REBUSAN AKAR ARU (Caesalpinia bonduc) TERHADAP KUALITAS SPERMA EPIDIDIMIS MENCIT (Mus musculus) : DASAR PENGEMBANGAN OBAT K0NTRASEPSI TRADISIONAL BAGI LAKI-LAKI

D. Setiadi dan S. Bachri

Abstract

Abstrak
Indonesia memiliki banyak tumbuhan berpotensi obat salah satunya bisa dijadikan sebagai obat
kontrasepsi tradisonal yang biasa digunakan untuk menjarangkan anak atau sterilisasi seperti
rebusan akar Caesalpinia bonduc (Aru). Tujuan penelitian untuk mengetahui pengaruh obat
kontrasepsi tradisional air rebusan akar aru terhadap kualitas pematangan sperma epididimis mencit
(Mus musculus). Mencit dipilih secara acak untuk mewakili 4 kelompok dosis yaitu : 0,0 (K0), 16,0
(K1) 24,0 (K2) dan 32,0 (K3) μl/gram berat badan/hari. Setiap kelompok perlakuan dilakukan
dengan 5 kali replikasi. Perlakuan diberikan melalui oral dengan menggunakan jarum gavage selama
10 hari berturut-turut. Pembedahan dilakukan pada hari pertama setelah perlakuan selesai.
Pengamatan kualitas sperma epididimis dengan perhitungan produksi sperma, % sperm motil, %
sperma hidup, % sperma abnormal,dan rangking keaktifan sperma. Untuk mengetahui ada atau
tidaknya perbedaan yang bermakna antar perlakuan dalam kelompok dilakukan uji Anava satu arah,
bila terdapat perbedaan bermakna dilanjutkan uji BNJ untuk membandingkan angka rata-rata antar
kelompok perlakuan. Pemberian air rebusan akar Caesalpinea bonduc pada mencit menunjukan
pengaruh perbedaan secara signifikan pada jumlah (%) sperma abnormal antara dosis 0,0 μl dengan
24,0 dan 32,0 μl serta antara 16,0 μl dengan 24,0 dan 32,0 μl; Jumlah (%) sperma hidup terdapat
perbedaan antara dosis 0,0 μl dengan 16,0 , 24,0 dan 32,0 μl; Jumlah (%) sperma motil terdapat
perbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl; Rangking keaktifan sperma terdapat
perbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl. Pemberian rebusan akar aru berpengaruh
secara signifikan terhadap peningkatan persen sperma abnormal, dan penuruan persen sperma hidup,
motil dan keaktifan.
Abstract
Indonesia has many species of plants that have potent of medicine, one of them cold be as an
traditional contraception medicine ussually used to limit child or sterilizatiom such as root boiled
water of Caesalpinia bonduc. The aims of this study is to know the effect of root boiled water of
Caesalpinia bonduc as traditional contraception medicine on quality of sperm maturation of
epididymis of mice (Mus musculus). Mice were choosed radomly and doses gouped: 0,0 (K0 ), 16,0
(K1) 24,0 (K2) and 32,0 (K3) μl/gram body weight/day. Each of group was replicated 5 times.
Treatment were given by oral with using gavage needle for ten days. Surgery was carried out on first
day after completing treatments. Examination of epidymis sperm by counting number of sperm
production, percentage of motil, live, abnormal sperm, and rank of sperm motility. In order to know
the deferences between control and treated group, was used one way anava analysis and analysed
value for comparing between teratment group. The treatments of root boiled water of Caesalpinia
bonduc have effect significantly on percentage of abnormal sperm between dose of 0,0 μl with 24,0
and 32,0 μl, also between 16,0 μl with 24,0 and 32,0 μl. Percentage of live sperem is different between
dose of 0,0 μl with 16,0 , 24,0 and 32,0 μl. Percentage of motile sperm is defferent significantly
between dose of 0,0 μl with 24,0 and 32,0 μl. Meanwhile percentage of motile rank has difference
between dose of 0,0 μl with 24,0 and 32,0 μl. The treatment of root boiled water of Caesalpinia
bonduc has effect signifiantly on increasing percentange of sperm abnormality, decreasing
percentage of life sperm, motile and rank of motile of mice sperm.

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