In Vitro Growth Response of Porang (Amorphophallus muelleri Blume) Bulbil to Kinetin Concentration
Authors
Rut NormasariDOI:
10.29303/jbt.v25i2.9050Published:
2025-05-25Issue:
Vol. 25 No. 2 (2025): April-JuniKeywords:
Porang, bulbil, kinetin, shoot, in vitro.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
The plant known as porang (Amorphophallus muelleri Blume) has a high glucomannan content in its tubers. The availability of porang seeds is one of the elements influencing porang production, hence tissue culture must be developed to improve seed procurement. For porang seed propagation to be successful in vitro, growth regulators must be added. This study aims to determine the effect of adding kinetin concentration on porang shoot induction. Five treatments the addition of kinetin at doses of 0, 1, 2, 3, and 4 mg/L were included in the Non-Factorial Completely Randomized Design (CRD) experiment. All observation variables, including explant growth, shoot emergence time, percentage of shoots created, total number of shoots, and number of shoots per explant, were found to be significantly impacted by the addition of kinetin. With a shoot emergence time of 21.86 days after inoculation, 96% of the shoots produced, a total number of 108.80 shoots, and a number of shoots of 22.75 per explant, kinetin 4 mg/L was the optimal concentration for porang shoot induction.
References
Abu-Romman, S. M., Al-Hadid, K. A., & Arabiyyat, A. R. (2015). Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber. Journal of Agricultural Science, 7(10), 159–165. https://doi.org/10.5539/jas.v7n10p159
Azizi, I., & Kurniawan, F. (2021). Pengaruh Bibit Asal, Umur, dan Ukuran terhadap Kadar Glukomanan dan Kadar Oksalat dalam Umbi Porang. Jurnal Sains Dan Seni ITS, 9(2). https://doi.org/10.12962/j23373520.v9i2.58571
Barciszewski, J., Siboska, G. E., Pedersen, B. O., Clark, B. F. C., & Rattan, S. I. S. (1997). A Mechanism for the In Vivo Formation of N6-Furfuryladenine, Kinetin, as A Secondary Oxidative Damage Product of DNA. FEBS Letters, 414(2), 457–460. https://doi.org/10.1016/S0014-5793(97)01037-5
Boer, D., Prawansa, A., Rakian, T. C., Arsyad, M. A., Arif, N., Madiki, A., & Arsana, I. M. W. (2024). Induksi Tunas Pisang Kepok (Musa paradisiaca L.) Menggunakan Kombinasi Kinetin dan IAA Secara In Vitro. Jurnal Agroteknos, 14(2), 55–60. https://doi.org/10.56189/ja.v14i2.49085
Ferziana, Erfa, L., Maulida, D., Sari, R. M., & Yuniardi, F. (2021). In Vitro Regeneration of Porang (Amorphophallus muelleri Blume) at Several Concentrations of BAP (Benzyl Amino Purine). International Conference on Agriculture and Applied Science (ICoAAS) 2021, 77–83. https://doi.org/10.25181/icoaas.v2i2.2486
Gantait, S., Kundu, S., & Das, P. K. (2018). Acacia: An Exclusive Survey on In Vitro Propagation. Journal of the Saudi Society of Agricultural Sciences, 17(2), 163–177. https://doi.org/10.1016/j.jssas.2016.03.004
Gubiš, J., Lajchová, Z., Faragó, J., & Jureková, Z. (2003). Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro. Czech Journal of Genetics and Plant Breeding, 39(1), 9–14. https://doi.org/10.17221/3715-CJGPB
Haring, F., Farid, M., Ridwan, I., Fadhilah, A. N., & Sedayu, N. (2024). Concentration of 2,4-D and BAP Combination on Callus Induction of Porang Plant (Amorphophallus muelleri Blume). Asian Journal of Plant Sciences, 23(3), 313–320. https://doi.org/10.3923/ajps.2024.313.320
Hidayah, N., Suhartanto, M. R., & Santosa, E. (2018). Pertumbuhan dan Produksi Benih Iles-iles (Amorphophallus muelleri Blume) Asal Teknik Budi Daya yang Berbeda. Buletin Agrohorti, 6(3), 386. https://doi.org/10.29244/agrob.6.3.386-392
Hosiana, A. M., Pinatih, G. N. I., & Laksemi, D. A. A. S. (2023). Beneficial Health Effects of Porang (Amorphophallus muelleri): A Review. Indonesia Journal of Biomedical Science, 17(2), 235–238. https://doi.org/10.15562/ijbs.v17i2.484
Ibrahim, M. S. D. (2019). Perbanyakan Iles-iles (Amorphophallus spp.) secara Konvensional dan Kultur In Vitro serta Strategi Pengembangannya. Perspektif, 18(1), 67. https://doi.org/10.21082/psp.v18n1.2019.67-78
Ibrahim, M. S. D., Sulistiyorini, I., & Tresniawati, C. (2022). Effect of 6-Benzyl Amino Purine on the Multiplication Ability of Shoots of Various Sizes of Porang (Amorphophallus muelleri Blume) Bulbils. IOP Conference Series: Earth and Environmental Science, 974(1), 012091. https://doi.org/10.1088/1755-1315/974/1/012091
Ibrahim, M. S. D., Sulistiyorini, I., Tresniawati, C., Izzah, N. K., & Hartati, Rr. S. (2024). Evaluation of Germination and Multiplication of Micro Shoot of Porang (Amorphophallus muelleri Blume) In Vitro. AIP Conference Proceedings 2957, 040033. https://doi.org/10.1063/5.0184596
Ikayanti, F., Radian, & Rianto, F. (2021). Pertumbuhan dan Hasil Tanaman Porang Periode Pertanian Pertama pada Tanah Gambut dengan Pemberian Pupuk NPK. Jurnal Pertanian Agros, 23(2), 319–326. https://doi.org/10.37159/jpa.v25i4.3528
Kumar, S., Chandra, A., & Gupta, M. G. (2008). Plantlet Regeneration via Multiple Shoot Induction in Indian Cultivars of Lucerne (Medicago sativa L). Journal of Plant Biochemistry and Biotechnology, 17(2), 181–184. https://doi.org/10.1007/BF03263282
Kumar, S., Singh, R., Kalia, S., Sharma, S., & Kalia, A. K. (2016). Recent Advances in Understanding the Role of Growth Regulators in Plant Growth and Development in Vitro-I. Conventional Growth Regulators. Indian Forester, 142(5), 459–470. https://doi.org/10.36808/if%2F2016%2Fv142i5%2F95052
Magyar-Tábori, K., Dobránszki, J., Teixeira da Silva, J. A., Bulley, S. M., & Hudák, I. (2010). The Role of Cytokinins in Shoot Organogenesis in Apple. Plant Cell, Tissue and Organ Culture (PCTOC), 101(3), 251–267. https://doi.org/10.1007/s11240-010-9696-6
Nugroho, K., Kosmiatin, M., Santoso, T. J., Sukma, D., Purwito, A., Husni, A., & Martasari, C. (2024). Optimization of Kinetin Concentrations and Medium Compositions for Citrus Shoot Multiplication from Cotiledonary Node. BIOTROPIA, 31(1), 96–105. https://doi.org/10.11598/btb.2024.31.1.2136
Pasternak, T. P., & Steinmacher, D. (2024). Plant Growth Regulation in Cell and Tissue Culture In Vitro. Plants, 13(2), 327. https://doi.org/10.3390/plants13020327
Pati, P. K., Rath, S. P., Sharma, M., Sood, A., & Ahuja, P. S. (2006). In Vitro Propagation of Rose—A Review. Biotechnology Advances, 24(1), 94–114. https://doi.org/10.1016/j.biotechadv.2005.07.001
Poerba, Y. S., Imelda, M., Wulansari, A., & Martanti, D. (2016). Induksi Mutasi Kultur In Vitro Amorphophallus muelleri Blume dengan Irradiasi Gamma. Jurnal Teknologi Lingkungan, 10(3), 355. https://doi.org/10.29122/jtl.v10i3.1482
Purnomo, M. H., Rizal, R., & Sundari, S. (2022). Status Keberlanjutan Usahatani Agroforestry Tanaman Porang pada Lembaga Masyarakat Desa Hutan Kemuning Asri di Gombengsari Kalipuro. Jurnal Agrinika : Jurnal Agroteknologi Dan Agribisnis, 6(1), 45. https://doi.org/10.30737/agrinika.v6i1.2143
Putri, A. D. P. L., Wiendi, N. M. A., & Santosa, E. (2023). Proliferation of Porang (Amorphophallus muelleri Blume) from Bulbils and Leaf Cutting Treated by NAA and BA. Jurnal Agronomi Indonesia, 51(3), 299–311. https://doi.org/10.24831/ija.v51i3.48958
Rezanejad, F., Abarian, M., & Abdirad, S. (2021). Shoot and Root Induction and Growth of Single Nodes of Rosa damascena in Different Culture Media. Journal of Plant Physiology and Breeding, 11(2), 97–108. https://doi.org/10.22034/JPPB.2021.14576
Royani, J. I., Chotimah, S., Utami, R. N., Fatma, W. S., Susiyanti, & Fatmawaty, A. P. (2021). Effect of Benzilaminopurine and Kinetin for shoot multiplication of Indigofera (Indigofera zollingeriana Miq.) by in vitro culture. IOP Conference Series: Earth and Environmental Science, 637(1), 012053. https://doi.org/10.1088/1755-1315/637/1/012053
Santosa, E. (2015). Pengembangan Tanaman Iles-Iles Tumpangsari untuk Kesejahteraan Petani dan Kemandirian Industri Pangan Nasional. RISALAH KEBIJAKAN PERTANIAN DAN LINGKUNGAN: Rumusan Kajian Strategis Bidang Pertanian Dan Lingkungan, 1(2), 73. https://doi.org/10.20957/jkebijakan.v1i2.10288
Sari, R., & Suhartati. (2015). Tumbuhan Porang: Prospek Budidaya sebagai Salah Satu Sistem Agroforestry. Info Teknis EBONI, 12(2), 97–110. https://doi.org/10.20886/buleboni.5061
Sompornpailin, K., & Khunchuay, C. (2016). Synergistic Effects of BAP and Kinetin Media Additives on Regeneration of Vetiver Grass (Vetiveria zizanioides L. Nash). Australian Journal of Crop Science, 10(05), 726–731. https://doi.org/10.21475/ajcs.2016.10.05.p7439
Sripaoraya, S., Marchant, R., Power, J. B., & Davey, M. R. (2003). Plant Regeneration by Somatic Embryogenesis and Organogenesis in Commercial Pineapple (Ananas comosus L.). In Vitro Cellular & Developmental Biology - Plant, 39(5), 450–454. https://doi.org/10.1079/IVP2003445
Sukartono, Suwardji, Kusumo, B. H., Bakti, A. A., & Edwin. (2020). Penguatan Kapasitas Kelompok Tani Dalam Budidaya Porang Berbasis Pertanian Konservasi-Agroforestry Di Desa Sambi Elen, Lombok Utara. Jurnal SIAR ILMUWAN TANI, 1(2), 67–74. https://doi.org/10.29303/jsit.v1i2.19
Sumarwoto. (2005). Iles-iles (Amorphophallus muelleri Blume); Deskripsi dan Sifat-sifat Lainnya. Biodiversitas Journal of Biological Diversity, 6(3), 185–190. https://doi.org/10.13057/biodiv/d060310
Tajuddin, M., Santosa, E., Sopandie, D., & Lontoh, A. P. (2020). Characteristics of growth, flowering and corm yield of iles-iles (Amorphophallus muelleri) genotypes at third growing period. Biodiversitas Journal of Biological Diversity, 21(2). https://doi.org/10.13057/biodiv/d210219
Tilaar, W., Rantung, J., & Tulung, S. (2015). Induksi Tunas dari Nodul Krisan Kulo dalam Media Murashige dan Skoog yang Diberi Sitokinin. EUGENIA, 21(2). https://doi.org/10.35791/eug.21.2.2015.9713
Van Khiem, D., Xuan Huyen, P., Thanh Hang, N. T., & Phuong Hoang, N. T. (2022). In Vitro Regeneration and Acclimatization of Anthurium scherzerianum Schott Plants. Academia Journal of Biology, 44(3), 99–109. https://doi.org/10.15625/2615-9023/16548
Verbeke, P., Siboska, G. E., Clark, B. F. C., & Rattan, S. I. S. (2000). Kinetin Inhibits Protein Oxidation and Glycoxidation In Vitro. Biochemical and Biophysical Research Communications, 276(3), 1265–1270. https://doi.org/10.1006/bbrc.2000.3616
Wardani, N. E., Subaidah, W. A., & Muliasari, H. (2021). Ekstraksi dan Penetapan Kadar Glukomanan dari Umbi Porang (Amorphophallus muelleri Blume) Menggunakan Metode DNS. Jurnal Sains Dan Kesehatan, 3(3), 383–391. https://doi.org/10.25026/jsk.v3i3.574
Yadav, R., Yadav, N., & Kumar, S. (2016). An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 86(4), 921–929. https://doi.org/10.1007/s40011-015-0550-9
License
Copyright (c) 2025 Rut Normasari

This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.