TY - JOUR AU - Hasan Basri, Muhammad AU - Zulkifli, Lalu AU - Syukur, Abdul PY - 2021/01/20 Y2 - 2024/03/29 TI - Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria JF - Jurnal Biologi Tropis JA - JBT VL - 21 IS - 1 SE - Articles DO - 10.29303/jbt.v21i1.2340 UR - https://jurnalfkip.unram.ac.id/index.php/JBT/article/view/2340 SP - 72-80 AB - <pre><span>Plant damage by pathogenic fungi is often found in plants, one of which is caused by <em>Sclerotium rolfsii</em>. Biological control strategy offers a promising alternative for managing disease in plants because they are environmental friendly compared to pesticides application. One of the biological control offered is by using endophytic fungi isolated from <em>Vitex trivolia</em> L. The aim of the study was to isolate, to identify macroscopic and microscopic endophytic fungi from <em>Vitex trifolia</em> L and to test their antagonism potency against the pathogenic fungus <em>Sclerotium rolfsii</em> in vitro. The isolation obtained 7 endophytic fungi isolates identified based on their genus characteristics, nsmely </span><em><span>Periconia </span></em><span>sp, <em>Aspergillus</em>sp, <em>Dendrophoma  </em>sp, <em>Geotrichum  </em>sp, <em>Ampulliferina  </em>sp, <em>Chalara  </em>sp, dan <em>Bispora </em>sp </span><span>and 2 isolates have not been identified. The Antibacterial test of the fungi isolate on the 4 tested bacteria showed that of all the fungi isolate have low activity. The antagonism test using the direct opposition method with the PIRG formula, showed that the 3 isolates had high percentage of growth inhibition, in which ALJ1, BLJ5, and ALJ3 isolate has  85%, 90%, and 100% respectively. This potency could be used as biological agents on the pathogenic fungus <em>Sclerotium rolfsii</em>.</span></pre> ER -