Antibacterial Activity of Sumbawa Forest Honey (Apis dorsata) against Klebsiella pneumoniae ATCC BA1705
DOI:
10.29303/jbt.v25i4a.10159Published:
2025-11-10Downloads
Abstract
Klebsiella pneumoniae is an opportunistic bacterium often associated with antibiotic resistance, necessitating the investigation of natural antibacterial alternatives. Honey is recognized for its bioactive ingredients that possess antibacterial properties. This research sought to assess the antibacterial efficacy of Sumbawa forest honey (Apis dorsata) against K. pneumoniae ATCC BA1705 by inhibition zone test. A laboratory experiment was performed using the well diffusion technique. K. pneumoniae suspensions (McFarland 0.5) were injected onto Mueller Hinton Agar. Wells (Ø 6 mm) were filled with 100 µL of honey at concentrations of 25%, 50%, 75%, and 100%. Tetracycline (1 µg/µL) acted as the positive control, while distilled water functioned as the negative control. Plates were incubated at 35±2°C for a duration of 18 to 24 hours. Inhibition zones were quantified using a caliper, and each experiment was conducted in sextuplicate. Data were expressed as mean ± standard deviation. No inhibitory zones were found at 25% and 50% concentrations (0 mm). Antibacterial activity was seen at 75% concentration, yielding a mean diameter of 10.33±SD mm, and raised to 100% concentration, resulting in a mean diameter of 11.33±SD mm. Tetracycline generated a 26 mm inhibitory zone, while distilled water exhibited none (0 mm). The conclusion is Sumbawa forest honey exhibits concentration-dependent antibacterial activity against K. pneumoniae, with moderate inhibition at concentrations ≥75%. Although less effective than tetracycline, these findings support the potential role of honey as a complementary antibacterial agent.
Keywords:
Sumbawa honey, Apis dorsata, Klebsiella pneumoniae, inhibition zone, well diffusion.References
Abbas, R., et al. (2024). General overview of Klebsiella pneumoniae: epidemiology and the role of siderophores in its pathogenicity. Biology, 13(2): 78. https://doi.org/10.3390/biology13020078
Abu Bakar, M.H. (2024). Antibacterial activity of Malaysian Trigona itama and Trigona thoracica honey against Gram-negative and Gram-positive bacteria. Qeios [Preprint]. https://doi.org/10.32388/JEYD13
Adelina Saputri, N., et al. (2023). Analisis pengaruh suhu terhadap warna, densitas, dan viskositas madu hutan lebah Apis dorsata dari Kecamatan Lunyuk. Jurnal Pengolahan Pangan, 8(1): 1–8. https://doi.org/10.31970/pangan.v8i1.79
Ahmad, A., et al. (2015). Pharmacodynamic modelling of in vitro activity of tetracycline against a representative, naturally occurring population of porcine Escherichia coli. Acta Veterinaria Scandinavica, 57(1): 1-8. https://doi.org/10.1186/s13028-015-0169-0
Al-Kafaween, M.A., et al. (2023). Physicochemical characteristics and bioactive compounds of different types of honey and their biological and therapeutic properties: a comprehensive review. Antibiotics, 12(2): 1-34. https://doi.org/10.3390/antibiotics12020337
Bacayo, M.F.D.C., et al. (2022). A comparative study of the antimicrobial activity of wild (Tualang) honey and artificial honey against methicillin-resistant Staphylococcus aureus, Streptococcus pneumoniae and Klebsiella pneumoniae. In: BROMO 2018 – Bromo Conference, Symposium on Natural Products and Biodiversity. SCITEPRESS – Science and Technology Publications, Lda., pp. 1-5. https://doi.org/10.5220/0009842900002406
Badan Pusat Statistik. (2022). Statistik Produksi Kehutanan 2022. Badan Pusat Statistik, Jakarta.
CLSI. (2024). CLSI M100: Performance standards for antimicrobial susceptibility testing. 34th ed. Clinical and Laboratory Standards Institute, Wayne, PA.
Cui, Z.H., et al. (2021). Rapid screening of essential oils as substances which enhance antibiotic activity using a modified well diffusion method. Antibiotics, 10(4): 1-11. https://doi.org/10.3390/antibiotics10040463
Dong, N., et al. (2022). Klebsiella species: taxonomy, hypervirulence and multidrug resistance. eBioMedicine, 79: 1-11. https://doi.org/10.1016/j.ebiom.2022.103998
Hewett, S.R., et al. (2022). Both manuka and non-manuka honey types inhibit antibiotic resistant wound-infecting bacteria. Antibiotics, 11(8): 1-14. https://doi.org/10.3390/antibiotics11081132
Karami-Zarandi, M., et al. (2023). Klebsiella pneumoniae: an update on antibiotic resistance mechanisms. Future Microbiology, 18(1): 65–81. https://doi.org/10.2217/fmb-2022-0097
LaPlante, K.L., et al. (2022). Re-establishing the utility of tetracycline-class antibiotics for current challenges with antibiotic resistance. Annals of Medicine, 54(1): 1686–1700. https://doi.org/10.1080/07853890.2022.2085881
Liaqat, I., et al. (2022). Honey potential as antibiofilm, antiquorum sensing and dispersal agent against multispecies bacterial biofilm. Journal of Oleo Science, 71(3): 425–434. https://doi.org/10.5650/jos.ess21199
Luitel, P., et al. (2023). Anti-bacterial activity of different honey samples against bacteria isolated from clinical sources. Tribhuvan University Journal of Microbiology, 10(1): 21–29. https://doi.org/10.3126/tujm.v10i1.60647
Mustafa, G., et al. (2022). Antibacterial properties of Apis dorsata honey against some bacterial pathogens. Saudi Journal of Biological Sciences, 29(2): 730–734. https://doi.org/10.1016/j.sjbs.2021.09.059
Nirwana, A.P. & Silviani, Y. (2022). Effectiveness of Jambi forest honey and klanceng honey in inhibiting Escherichia coli and Klebsiella pneumoniae ESBL. Jurnal Surya Medika, 8(3): 120–126. https://doi.org/10.33084/JSM.V8I3.3317
Nurhasanah Sari, D., et al. (2023). Karakteristik madu hutan lebah Apis dorsata daerah Sulawesi Tenggara ditinjau dari sifat fisika-kimia. Cokroaminoto Journal of Chemical Science, 5(2): 42–46. https://science.e-journal.my.id/cjcs/article/view/163 (Accessed: April 16, 2024)
Prastyowati, A., et al. (2021). Biodiversity of lactic acid bacteria of wild honey bee and Sumbawa horse milk by using RAPD-PCR. In: IOP Conference Series: Earth and Environmental Science. IOP Publishing, 762: 012001. https://doi.org/10.1088/1755-1315/762/1/012001
Putri, R.N., et al. (2023). Uji daya hambat antimikroba secara difusi sumuran dan difusi paper disk. Era Sains: Journal of Science, Engineering and Information Systems Research, 1(4): 28-33.
Qamar, M.U., et al. (2018). In vitro and in vivo activity of manuka honey against NDM-1-producing Klebsiella pneumoniae ST11. Future Microbiology, 13(1): 13–26. https://doi.org/10.2217/fmb-2017-0119
Saputri, N.A., et al. (2023). Analisis pengaruh suhu terhadap warna, densitas, dan viskositas madu hutan lebah Apis dorsata dari Kecamatan Lunyuk-Sumbawa. Jurnal Pengolahan Pangan, 8(1): 1–8. https://doi.org/10.31970/PANGAN.V8I1.79
Singh, P. & Sillanpaa, M. (2023). Degradation of antibiotics and antibiotic-resistant bacteria from various sources. Elsevier, Amsterdam.
Talebi, M., et al. (2020). Molecular mechanism-based therapeutic properties of honey. Biomedicine and Pharmacotherapy, 130: 110590. https://doi.org/10.1016/j.biopha.2020.110590
Wadi, M.A. (2022). In vitro antibacterial activity of different honey samples against clinical isolates. BioMed Research International, 2022: 1560050. https://doi.org/10.1155/2022/1560050
Wang, G., et al. (2020). The characteristic of virulence, biofilm and antibiotic resistance of Klebsiella pneumoniae. International Journal of Environmental Research and Public Health, 17(17): 6287. https://doi.org/10.3390/ijerph17176278
Wijayanti, N., et al. (2022). Karakteristik habitat lebah (Apis dorsata), teknik pemanenan madu hutan, serta karakteristik madu hutan Sumbawa di Kabupaten Sumbawa, Indonesia. Journal of Global Sustainable Agriculture, 3(1): 14. https://doi.org/10.32502/jgsa.v3i1.5291
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