Optimization of Functional Properties of Cocoyoghurt through Fortification with Moringa Leaf Extract (Moringa oleifera): Antioxidant Activity and Organoleptic Characteristics
DOI:
10.29303/jpm.v20i6.9524Published:
2025-09-24Issue:
Vol. 20 No. 6 (2025): in ProgressKeywords:
Coconut; Yoghurt; Moringa Leaf; Functional FoodArticles
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Abstract
Yogurt is a fermented milk product known as a functional food. Food diversification efforts by finding alternative yogurt raw materials to replace animal milk are necessary to provide products for people who want alternative non-dairy yogurt products. One of the plant-based milks that has a nutritional content similar to animal milk is coconut (Cocos nucifera). Moringa leaves (Moringa oleifera) are reported to contain antioxidant compounds and have therapeutic benefits, making them potentially suitable for use as a fortifier in cocoyogurt functional drinks. The purpose of this study was to obtain the most appropriate formula for adding moringa leaf extract to produce cocoyogurt products that are high in antioxidants and have physicochemical and organoleptic properties that are acceptable to consumers. The design used in this study was a completely randomized design (CRD) with 4 treatments, namely: P0 = without the addition of moringa leaf extract; P1 = moringa leaf extract 1 g/L; P2 = moringa leaf extract 2 g/L; and P3 = moringa leaf extract 3 g/L. The data obtained were analyzed using the Analysis of Variance (ANOVA) statistical method and continued with Duncan's Multiple Range Test if there was a significant difference (P ≤ 0.05) between treatments. Based on the results of the study, it is known that the addition of moringa leaf extract to coconut milk yogurt produces cocoyoghurt with a low acidity (pH) (4.5-44.3), which is in accordance with the SNI standard (2981-2009) for yogurt quality. Overall, the best treatment in cocoyoghurt was the addition of 3 g/L of moringa leaf extract with an IC50 value of 148.33 µg/ml, with free radical inhibitory activity in the moderate category, and the sensory properties of taste, aroma, color, and texture showed the most preferred values by the panellists. These findings have broader implications for functional food innovation, particularly in developing plant-based, nutrient-enriched products that cater to lactose-intolerant populations and health-conscious consumers seeking natural sources of antioxidants.
References
References
X. Lu, H. Su, J. Guo, J. Tu, Y. Lei, S. Zeng, and B. Zheng, "Rheological properties and structural features of coconut milk emulsions stabilized with maize kernels and starch, "Food Hydrocolloids, vol. 96, pp. 385–395, 2019, doi: https://doi.org/10.1016/j.foodhyd.2019.05.027
A. Narayanankutty, S. P. Illam, and A. C. Raghavamenon, "Health impacts of different edible oils prepared from coconut (Cocos nucifera): A comprehensive review, "Trends in Food Science & Technology, vol. 80, pp. 1–7, 2018, doi: https://doi.org/10.1016/j.tifs.2018.07.025
E. Topcuoglu and L. Yilmaz‑Ersan, “Evaluation of instrumental and sensory measurements using multivariate analysis in probiotic yogurt enriched with almond milk,” Journal of Food Science and Technology, vol. 59, no. 1, pp. 133–143, Feb. 2021, doi: https://doi.org/10.1007/s13197-021-04994-w
[T. Zhang, C. H. Jeong, W. N. Cheng, H. Bae, H. G. Seo, M. C. Petriello, and S. G. Han, “Moringa extract enhances the fermentative, textural, and bioactive properties of yogurt,” LWT – Food Science and Technology, vol. 101, pp. 276–284, 2019, doi: http://dx.doi.org/10.1016/j.lwt.2018.11.010
Kumari M, Kokkiligadda A, Dasriya V, Naithani H. Functional relevance and health benefits of soymilk fermented by lactic acid bacteria. J Food Sci Technol. 2022 Apr;59(4):1263–1279, doi: https://doi.org/10.1111/jam.15342
E. Fachriyaha, D. Kusrinia, and I. B. Haryanto, "Phytochemical Test, Determination of Total Phenol, Total Flavonoids and Antioxidant Activity of Ethanol Extract of Moringa,"J. Kimia Sains dan Aplikasi, vol. 23, no. 8, pp. 290–294, 2020, doi: https://doi.org/10.14710/jksa.23.8.290-294
S. H. Dehshahri, M. Wink, S. Afsharypuor, G. Asghari, and A. Mohagheghzadeh, "Antioxidant activity of methanolic leaf extract of Moringa peregrina (Forssk.) Fiori,"Res. Pharm. Sci., vol. 7, no. 2, pp. 111–117, 2012, doi: https://pmc.ncbi.nlm.nih.gov/articles/PMC3501899/
S. M. Gomes, A. Leitão, A. Alves, and L. Santos, "Incorporation of Moringa oleifera leaf extract in yoghurts to mitigate children’s malnutrition in developing countries,"Molecules, vol. 28, no. 6, p. 2526, 2023,doi: https://doi.org/10.3390/molecules28062526
Z. Raharjanti, Y. B. Pramono, and A. N. M. Al-Baarri, "Nilai pH dan Kekentalan Cocogurt dengan Penambahan Ekstrak Daun Stevia," J. Teknol. Pangan, vol. 3, no. 2, pp. 305–308, 2019, doi: https://doi.org/10.14710/jtp.2019.23874
P. D. I. Sari, Y. R. Swasti, and E. Purwijatiningsih, "Antioxidant Activity and Quality of Frozen Yogurt with Moringa oleifera Lamk. Leaves Extract," agriTECH, vol. 45, no. 1, pp. 31–40, doi: https://doi.org/10.22146/agritech.83392
K. Sanjaya et al., "Preparation and quality evaluation of yogurt by incorporation with Moringa oleifera leaves powder," Int. J. Nutr. Health, vol. 4, no. 11, pp. 2–8, 2020, doi: http://dx.doi.org/10.31080/ASNH.2020.04.0769
Z. Raharjanti, Y. B. Pramono, and A. N. Al-Baarri, "Nilai pH dan Kekentalan Cocogurt dengan Penambahan Ekstrak Daun Stevia," J. Teknol. Pangan, vol. 3, no. 2, pp. 305–308, 2019, doi: https://doi.org/10.14710/jtp.2019.23874
N. Ismawati, "Nilai pH, Total Padatan Terlarut, dan Sifat Sensoris Yoghurt Dengan Penambahan Ekstrak Bit (Beta vulgaris L.)," J. Apl. Teknol. Pangan, vol. 5, no. 3, pp. 89–93, 2016, doi: https://doi.org/10.17728/jatp.181
E. Rossi, F. Restuhadi, R. Efendi, and Y. K. Dewi, “Physicochemical and microbiological properties of yogurt made with microencapsulation probiotic starter during cold storage,” Biodiversitas, vol. 22, no. 7, pp. 2012–2018, 2021, doi: https://doi.org/10.13057/biodiv/d220450
P. R. Sintyadewi and W. K. Yoga, “Functional food innovation of yogurt from breadnut seeds (Artocarpus camansi): Basic formulation and quality analysis,” Jurnal Pijar MIPA, vol. 19, no. 6, pp. 1041–1046, 2024, doi: https://doi.org/10.29303/jpm.v19i2.6616
Y. Agustina, R. Kartika, and A. S. Panggabean, “Pengaruh variasi waktu fermentasi terhadap kadar laktosa, lemak, pH dan keasaman pada susu sapi yang difermentasi menjadi yogurt,” Jurnal Kimia Mulawarman, vol. 12, no. 2, pp. 97–100, 2015, doi: https://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/9
D. Yustendi, S. Wardani, and Mulyadi, “Pengaruh lama penyimpanan yogurt susu kambing dengan penambahan bakteri Streptococcus thermophilus dan bakteri Lactobacillus bulgaricus terhadap pH, protein dan bakteri asam laktat,” Jurnal Agriflora, vol. 5, no. 1, pp. 47–51, 2021, doi: https://doi.org/10.3061/unayaded.v5i1.1941
N. García‐González and M. J. López‐Ruiz, “The impact of physicochemical conditions on lactic acid bacteria survival in food products,” Fermentation, vol. 10, no. 6, p. 298, 2023, doi: https://doi.org/10.3390/fermentation10060298
D. A. Kusmardika, “Potensi aktivitas antioksidan daun kelor (Moringa oleifera) dalam mencegah kanker,” Journal of Health Science and Physiotherapy, vol. 2, no. 1, pp. 46–50, 2020., doi: https://doi.org/10.35893/jhsp.v2i1.33
P. Kashyap et al., “Recent advances in Drumstick (Moringa oleifera) leaves bioactive compounds: Composition, health benefits, bioaccessibility, and dietary applications,” Antioxidants, vol. 11, no. 2, p. 402, 2022, doi: https://doi.org/10.3390/antiox11020402
S. E. Kamal and M. Aris, “Aktivitas antioksidan ekstrak etanol 70% daun kelor (Moringa oleifera Lam.) terhadap DPPH,” Jurnal Pro-Life, vol. 8, no. 2, pp. 168–177, 2021, doi: https://doi.org/10.33541/pro-life.v8i2.3209
I. Oduro, W. O. Ellis, and D. Owusu, “Nutritional potential of two leafy vegetables: Moringa oleifera and Ipomoea batatas leaves,” Scientific Research and Essays, vol. 3, no. 2, pp. 57–60, 2008, doi: https://www.periodicos.capes.gov.br/index.php/acervo/buscador.html?task=detalhes&id=W2154208128
R. K. Saini, I. Sivanesan, and Y.-S. Keum, “Phenolic compounds of Moringa oleifera leaves and their antioxidant activity: A review,” Journal of Functional Foods, vol. 5, no. 4, pp. 1701–1710, 2014, doi: https://doi.org/10.1016/j.jff.2013.09.020
Gopalakrishnan, L., Doriya, K., & Kumar, D. S. “Moringa oleifera: A review on nutritive importance and its medicinal application,” Food Science and Human Wellness, vol 5, no. 2, pp. 49–56, 2016, doi: https://doi.org/10.1016/j.fshw.2016.04.001
Kumar, S., Yadav, S. K., Yadav, A. N., & Kumar, A. “Development of moringa enriched functional fermented dairy product using probiotic lactic acid bacteria,” Journal of Food Science and Technology, vol 57, pp: 3795–3802, 2020, doi: https://doi.org/10.1007/s13197-020-04410-z
R. Ponka, P. M. Zhung, G. Zomegni, C. G. Tchouape, and E. Fokou, “Organoleptic and physicochemical properties of soy‐milk yoghurt enriched with Moringa oleifera root powder,” Global Challenges, vol. 6, no. 5, p. 2100097, 2022, doi: https://doi.org/10.1002/gch2.202100097
M. Aznury, Z. Zaman, A. Zikri, and P. Panzurli, “Pengujian organoleptik produk yogurt dengan penambahan variasi konsentrasi daun kelor (*Moringa oleifera*),” *Fluida*, vol. 12, no. 1, pp. 15–20, 2019, doi: https://doi.org/10.35313/fluida.v12i1.1844
Azis R, Lestari JW, Puspaningsih ES, Muqtafiah. Fortification of Yogurt with Moringa Leaf Extract (Moringa oleifera) on Quality and Quality as a Functional Food: A Review. Int J Sustain Soc Cult Sci Technol Manag Law Humanit. Vol 2, no.1, pp. 24–32, 2025, doi: http://dx.doi.org/10.71131/3a431y62
Author Biographies
Putu Rima Sintyadewi, Bachelor of Food Technology Program, Faculty of Technology, Institute of Technology and Health Bali
Ida Ayu Putu Ary Widnyani, Bachelor of Food Technology Program, Faculty of Technology, Institute of Technology and Health Bali
Anak Agung Ngurah Dwi Ariesta Wijaya Putra, Bachelor of Food Technology Program, Faculty of Technology, Institute of Technology and Health Bali
Nadya Treesna Wulansari, Bachelor of Food Technology Program, Faculty of Technology, Institute of Technology and Health Bali
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Copyright (c) 2025 Putu Rima Sintyadewi, Ida Ayu Putu Ary Widnyani, Anak Agung Ngurah Dwi Ariesta Wijaya Putra, Nadya Treesna Wulansari

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