Experiment and Literature: Enzyme Content of Fruit Waste-Based Eco Enzyme and Its Potential in Pollutant Reduction
DOI:
10.29303/jbt.v25i4.10066Published:
2025-10-01Downloads
Abstract
Indonesia faces serious challenges in managing domestic waste, especially organic waste, which constitutes the largest component of the total national waste generation. One innovative form of recycling is the fermentation of organic waste into eco enzymes, which are fermented liquids capable of producing various active enzymes such as protease, amylase, and lipase with broad ecological benefits. This study aims to observe the activity of protease, amylase, and lipase enzymes in eco enzyme solutions made from fruit waste, and compare the results with previous studies to analyze the potential application of eco enzymes as efficient, economical, and sustainable natural bioremediation agents. The fermentation process was carried out for 90 days, while enzyme activity was analyzed using spectrophotometry and titration methods. The results showed protease activity of 261 U/mL, amylase of 21.53 U/mL, and lipase of 3.46 U/mL. These values are relatively high compared to several previous studies, indicating that fruit waste is an effective substrate for eco enzyme production. Thus, it can be concluded that eco enzyme has great potential in reducing pollutants such as heavy metals, BOD, COD, TSS, and helping to stabilize pH, thereby playing an important role in the treatment of domestic waste and small-scale industrial waste. The recommendation from this study is the need for further research related to the optimization of substrate composition.
Keywords:
Eco enzyme, enzymatic activity, fruit waste, pollutants.References
Anisa, N. (2022). Efektivitas eco enzyme dalam Menurunkan Kadar Logam Berat pada Air Lindi TPA [Skripsi, Universitas Negeri Semarang]. UNNES Repository. https://lib.unnes.ac.id/id/eprint/77909.
Fadlurrahman, & Aznury. (2022). Variasi Fungsi Penerapan Ekoenzim dari Limbah Organik: Tinjauan Literatur. Jurnal Selulosa, 12(02), 61–61. https://doi.org/10.25269/jsel.v12i02.373
Galintin, O. (2020). Production and characterization of eco enzyme produced from fruit and vegetable waste and its influence on the aquaculture sludge. Biointerface Research in Applied Chemistry, 10(6), 6313–6322. https://doi.org/10.33263/BRIAC106.63136322.
Gumilar et al., (2025). Characterization and Enzymatic Evaluation of Ecoenzyme Derived from Fruit and Vegetable Waste: An Effort to Achieve Zero Waste Concept. Baghdad Science Journal, vol 22. https://doi.org/10.21123/bsj.2024.10707
Iddrisu, Danso, F., Cheong, K.-L., Fang, Z., & Zhong, S. (2024). Polysaccharides as Protective Agents against Heavy Metal Toxicity. Foods, 13(6), 853–853. https://doi.org/10.3390/foods13060853
Indraloka, Istanti, & Utami. (2023). The physical and chemical characteristics of eco-enzyme fermentation liquids from several compositions of local fruits and vegetables in banyuwangi. IOP Conference Series, 1168(1), 012018–012018. doi: org/10.1088/1755-1315/1168/1/012018
Indraloka, R., Wibowo, A., & Nuraini, T. (2023). Evaluasi aktivitas enzim amilase pada eco enzyme berbahan dasar sawi dan kulit buah. IOP Conference Series: Earth and Environmental Science, 1168, 012018. https://doi.org/10.1088/17551315/1168/1/012018
Juniartini, N. L. P. (2020). Pengelolaan Sampah Dari Lingkup Terkecil dan Pemberdayaan Masyarakat sebagai Bentuk Tindakan Peduli Lingkungan. Jurnal Bali Membangun Bali, 1(1), 27–40. https://doi.org/10.51172/jbmb.v1i1.106
Kamila, Mulyadi, Suharti, Susanti, & Haryono. (2024). Enzymatic assay of coffee peel and papaya peel waste eco enzyme. Bio Web of Conferences/BIO Web of Conferences, 117, 01042–01042. https://doi.org/10.1051/bioconf/202411701042
Kementrian Lingkungan Hidup dan Kehutanan, (2022). Peraturan Menteri Lingkungan Hidup dan Kehutanan No.6 Tahun 2022.
Mardliyah, A. (2021). Pengaruh variasi substrat buah terhadap aktivitas enzim amilase pada eco enzyme. [Skripsi UIN Sunan Gunung Djati Bandung]. http://repository.uinsgd.ac.id/55555/
Muhammad. (2023). Karakterisasi Dan Uji Aktivitas Eksoenzim Limbah Buah Dan Sayuran - UPI Repository. Upi.edu. http://repository.upi.edu/108057/1/S_KIM_1908404_Title.pdf
Napitupulu, K. (2021). Degradation of Fruit and Vegetable Wastes in Ecoenzyme Production. Ipb.ac.id.http://repository.ipb.ac.id/handle/123456789/105772.
Pasaribu M. K., Damanik. W., Tampubolon U. N., Parapat L., & Purba B. T, (2025). Edukasi Pengelolaan Sampah Organik Untuk Peningkatan Kesehatan di Desa Bandar Tengah. 5(1). 175-175. https://doi.org/10.36378/bhakti_nagori.v5i1.4273.
Putri C. P., Widiarin R., Ramadhanintyas N. (2024). Effectiveness of Eco Enzymes in Reducing COD and TSS in Tofu Waste in Klumutan Village. IJSR International Journal Labs. 3(1), 45-52. P-ISSN: 2827-9832.
Rusdianasari, Syakdani, A., Zaman, M., Sari, F.F., Nasyta, N. P., & Amalia, R., (2021). Production of Disinfectant by Utilizing Eco-enzyme from Fruit Peels Waste. International Journal of Research in Vocational Studies, 1 (3), pp. 1-7. https://doi.org/10.53893/ijrvocas.v li3.53
Sari, G. L., Adzillah, W. N., Ratnawati, K., Sari, D. F. A., & Saidah, S. (2025). Analysis of Eco-Enzyme Potential to Reduce BOD, COD, Oil and Grease Concentrations in Raw Water for Treated Water in Karawang: A Laboratory Experiment. Gema Lingkungan Kesehatan, 23(1),6–13. https://doi.org/10.36568/gelinkes.v23i1.148
Satitiningrum, Y., Ulmillah, A., & Haryanti, Y. (2025). Potential of Proteolytic Bacteria from Vegetable and Fruit Waste-Based Eco-Enzyme. Jurnal Ilmiah Biologi Eksperimen Dan Keanekaragaman Hayati (J-BEKH), 12(1),37–52. https://doi.org/10.23960/jbekh.v12i1.405
Suliestyah, D., Rachmawati, A., & Hidayat, T. (2023). Evaluasi aktivitas amilase dan selulase pada eco enzyme dari kulit buah. International Research Journal of Engineering, IT & Scientific Research, 9(1), 85–92. https://sloap.org/journals/index.php/irjeis/article/view/2199
Superdianto. (2023). Perbandingan Kualitas Produk Eco-enzyme Dari Berbagai Kulit Buah. Uksw.edu. https://repository.uksw.edu//handle/123456789/29655
Sektion, M. S., & Mubarik, N. R. (2023). Isolasi bakteri amilolitik dari eco enzyme dan karakterisasi aktivitas enzim. Jurnal Ilmu Pertanian Indonesia, 28(3), 178–185. https://journal.ipb.ac.id/index.php/JIPI/article/view/42317
Soleha. (2023). Optimization of pH on Enzymatic Activity of Eco-Enzyme Averrhoa bilimbi L. in Plaju District, South Sumatra. Jurnal Biota, 9(2), 72–79. https://doi.org/10.19109/Biota.v9i2.16460
Scientia. (2024). Mikrobiologi - BM3207 - ITB - Studocu. Studocu. https://www.studocu.id/id/course/institut-teknologi-bandung/mikrobiologi/3673005?origin=document-viewer
Uswati, D. (2024). Karakterisasi dan uji aktivitas eco enzyme limbah buah dan sayuran. [Skripsi Universitas Sriwijaya].
https://repository.unsri.ac.id/137737/
Wibowo et al., 2022). Bakteri Penghasil Amilase Yang Diisolasi Dari Ekoenzim Limbah Buah-Buahan. Jurnal Bio Silampari, 4(2), 107–117. https://doi.org/10.31540/biosilampari.v4i2.1531
Wikaningrum, T., Fajriyah, N. N., & Nurulita, Y. (2022). Aplikasi Eco Enzyme dan EM1 pada Pengolahan Limbah Cair Menggunakan Metode Lumpur Aktif. Jurnal Urban Environmental Technology, 14(1), 12–21. https://doi.org/10.25105/urbanenvirotech.v14i1.15545.
Zamroni, M., Prahara, R. S., Kartiko, A., Purnawati, D., & Kusuma, D. W. (2020). The Waste Management Program Of 3R (Reduce, Reuse, Recycle) By Economic Incentive and Facility Support. Journal of Physics: Conference Series, 1471, 012048. https://doi.org/10.1088/1742-6596/1471/1/012048.
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