Identification of Thraustochytrids from Mangrove Forest of Tidung Island, Kepulauan Seribu, Jakarta
DOI:
10.29303/jbt.v25i4b.10133Published:
2025-12-05Downloads
Abstract
Thrautochytrids are often called heterotrophic microalgae that have a lot of omega-3 docosahexaenoic acid, or DHA. These microbes are found in many mangrove forest areas, including Indonesia, which has the biggest mangrove forests in the world. Indonesia is also beginning to look for useful microbes in these environments. The goal of this study was to find and identify a type of Thrautochytrid called Aurantiochytrium sp. in the mangrove forests of Tidung Island, part of the Thousand Islands near Jakarta. This area has never been studied for this type of microbe before. The study found two isolates of Thrautochytrids, named Aurantiochytrium sp. PTD5 and PTD6. These were identified by looking at the phylogenetic tree made from 18S rRNA sequences. The phylogenetic tree shows that PTD5 and PTD6 are closely related to other Aurantiochytrium strains like SR21 and BL10, which are widely used in research for making omega-3 DHA lipids.
Keywords:
Aurantiochytrium sp. Mangrove Forest Phylogenetic Analysis Thrautochytrids Tidung IslandReferences
Aasen, I. M., Ertesvåg, H., Heggeset, T. M. B., Liu, B., Brautaset, T., Vadstein, O., & Ellingsen, T. E. (2016). Thraustochytrids As Production Organisms For Docosahexaenoic Acid (DHA), Squalene, and Carotenoids. Applied Microbiology and Biotechnology 2016 100:10, 100(10), 4309–4321. https://doi.org/10.1007/S00253-016-7498-4
Abdullah, M. M., & Sembiring, L. (2009). Deteksi, Karakterisasi, dan Identifikasi Mikroalga Kontaminan Pada Polimer Emulsi Berbasis Polivinil Asetat (PVA). Prosiding Seminar Nasional Penelitian, Pendidikan Dan Penerapan MIPA, Fakultas MIPA, Universitas Negeri Yogyakarta, 130–136. https://fileserver.core.ac.uk/pdf/pdf/33509911.pdf
Aini, U. N., Lunprom, S., Reungsang, A., & Salakkam, A. (2022). Docosahexaenoic Acid (DHA) Production by Aurantiochytrium limacinum Using Cassava Pulp Hydrolysate as an Alternative Low-Cost Carbon Source. Frontiers in Marine Science, 9(August), 1–14. https://doi.org/10.3389/fmars.2022.985119
Anafarida, O., & Badruzsaufari. (2020). Analisis Filogenetik Mangga (Mangifera SPP.) Berdasarkan Gen 5,8S RRNA. ZIRAA’AH MAJALAH ILMIAH PERTANIAN, 45(2), 120–126. https://doi.org/10.31602/ZMIP.V45I2.3001
Barsanti, L., & Gualtieri, P. (2014). Algae Utilization. In Algae. https://doi.org/10.1201/b16544-10
Carmona, M. L., Naganuma, T., & Yamaoka, Y. (2014). Identification by HPLC-MS of Carotenoids of the Thraustochytrium CHN-1 Strain Isolated from the Seto Inland Sea. Bioscience, Biotechnology and Biochemistry, 67(4), 884–888. https://doi.org/10.1271/BBB.67.884
Dharmayanti, N. L. P. I. (2011). Filogenetika Molekuler: Metode Taksonomi Organisme Berdasarkan Sejarah Evolusi. Wartazoa, 21(1), 1–10.
Ganuza, E., Yang, S., Amezquita, M., Giraldo-Silva, A., & Andersen, R. A. (2019). Genomics, Biology and Phylogeny Aurantiochytrium acetophilum sp. nov. (Thraustrochytriaceae), Including First Evidence of Sexual Reproduction. Protist, 170(2), 209–232. https://doi.org/10.1016/j.protis.2019.02.004
Hutari, A., An Nisaa, R., Suhendra, S., Agustin, Y., & Ayunda, K. A. (2022). Exploration Of High Economic Value Microalgae in The Mangrove Area Of Pari Island, Seribu Islands, Jakarta. Jurnal Pembelajaran Dan Biologi Nukleus, 8(3), 662–672. https://doi.org/10.36987/jpbn.v8i3.3096
Jaritkhuan, S., & Suanjit, S. (2018). Species Diversity and Polyunsaturated Fatty Acid Content of Thraustochytrids From Fallen Mangrove Leaves in Chon Buri Province, Thailand. Agriculture and Natural Resources, 52(1), 24–32. https://doi.org/10.1016/J.ANRES.2018.05.002
Jaseera, K. V., Kaladharan, P., Vijayan, K. K., Sandhya, S. V., Antony, M. L., & Pradeep, M. A. (2019). Isolation and Phylogenetic Identification of Heterotrophic Thraustochytrids From Mangrove Habitats Along The Southwest Coast of India and Prospecting Their PUFA Accumulation. Journal of Applied Phycology, 31(2), 1057–1068. https://doi.org/10.1007/s10811-018-1627-7
Jeuck, A., & Arndt, H. (2013). A Short Guide to Common Heterotrophic Flagellates of Freshwater Habitats Based on the Morphology of Living Organisms. Protist, 164(6), 842–860. https://doi.org/10.1016/J.PROTIS.2013.08.003
Kalidasan, K., Vinithkumar, N. V., Peter, D. M., Dharani, G., & Dufossé, L. (2021). Thraustochytrids of Mangrove Habitats from Andaman Islands: Species Diversity, PUFA Profiles and Biotechnological Potential. Marine Drugs 2021, Vol. 19, Page 571, 19(10), 571. https://doi.org/10.3390/MD19100571
Moran, C. A., Morlacchini, M., Keegan, J. D., & Fusconi, G. (2018). The Effect of Dietary Supplementation With Aurantiochytrium limacinum On Lactating Dairy Cows In Terms Of Animal Health, Productivity, and Milk Composition. Journal of Animal Physiology and Animal Nutrition, 102(2), 576–590. https://doi.org/10.1111/JPN.12827
Oktafia, R. E., & Badruzsaufari. (2021). Analisis Filogenetik Garcinia Spp. Berdasarkan Sekuens Gen rRNA. Ziraa’ah: Majalah Ilmiah Pertanian, 46(2), 259–264. https://doi.org/10.31602/ZMIP.V46I2.4526
Otagiri, M., Khalid, A., Moriya, S., Osada, H., & Takahashi, S. (2017). Novel Squalene-Producing Thraustochytrids Found in Mangrove Water. Bioscience, Biotechnology, and Biochemistry, 81(10), 2034–2037. https://doi.org/10.1080/09168451.2017.1359485
Patel, A., Rova, U., Christakopoulos, P., & Matsakas, L. (2019). Simultaneous Production of DHA and Squalene from Aurantiochytrium sp. Grown on Forest Biomass Hydrolysates. Biotechnology for Biofuels, 12(1), 255. https://doi.org/10.1186/s13068-019-1593-6
Prabhakaran, P., Raethong, N., Nazir, Y., Halim, H., Yang, W., Vongsangnak, W., Hamid, A. A., & Song, Y. (2022). Whole Genome Analysis and Elucidation of Docosahexaenoic acid (DHA) Biosynthetic Pathway in Aurantiochytrium sp. SW1. Gene, 846, 146850. https://doi.org/10.1016/J.GENE.2022.146850
Promega Corporation. (2019). Technical Manual Wizard® Genomic DNA Purification Kit Wizard® Genomic DNA Purification Kit. Technical Bulletin, 1–19.
Purbani, D. C., Ambarwati, W., Kusuma, A. B., & Herliany, N. E. (2019). Identifikasi Mikroalga Laut Tambrauw, Papua Barat. Jurnal Ilmu Dan Teknologi Kelautan Tropis, 11(3), 777–791. https://doi.org/10.29244/jitkt.v11i3.25862
Ramos-Vega, A., Rosales-Mendoza, S., Bañuelos-Hernández, B., & Angulo, C. (2018). Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines. Frontiers in Microbiology, 9, 2506. https://doi.org/10.3389/fmicb.2018.02506
Sirirak, K., Suanjit, S., Powtongsook, S., & Jaritkhuan, S. (2020). Characterization and PUFA Production of Aurantiochytrium limacinum BUCHAXM 122 Isolated From Fallen Mangrove Leaves. ScienceAsia, 46(4), 403–411. https://doi.org/10.2306/scienceasia1513-1874.2020.051
Takahashi, S., Yoshida, M., Watanabe, M. M., & Isoda, H. (2019). Anti-Inflammatory Effects of Aurantiochytrium limacinum 4W-1b Ethanol Extract on Murine Macrophage RAW264 Cells. BioMed Research International, 2019. https://doi.org/10.1155/2019/3104057
Thatoi, H., Behera, B. C., Mishra, R. R., & Dutta, S. K. (2013). Biodiversity and Biotechnological Potential of Microorganisms From Mangrove Ecosystems: A Review. Annals of Microbiology, 63(1), 1–19. https://doi.org/10.1007/s13213-012-0442-7
Wang, Q., Ye, H., Sen, B., Xie, Y., He, Y., Park, S., & Wang, G. (2018). Improved Production of Docosahexaenoic Acid in Batch Fermentation by Newly-isolated Strains of Schizochytrium sp. and Thraustochytriidae sp. Through Bioprocess Optimization. Synthetic and Systems Biotechnology, 3(2), 121–129. https://doi.org/10.1016/J.SYNBIO.2018.04.001
Wangiyana, I. G. A. S. (2022). Rekonstruksi Filogenetik Dari Sekuen Maturase K Gyrinops versteegii Menggunakan Referensi Pencarian Megablast. Jurnal Sangkareang Mataram, 9(2), 19–24. https://sangkareang.org/index.php/SANGKAREANG/article/view/478
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