Growth Rate and Chlorophyll Content of Liberica Coffee Leaves (Coffea liberica L.) with Addition Rhizosphere Bacterial Microcapsules from Eruption Soil of Mount Sinabung

Kabul Warsito; Desi Sri Pasca Sari Sembiring; Indra Irawan; Bagus Kriswanda

doi:10.29303/jbt.v25i4.10221

Growth Rate and Chlorophyll Content of Liberica Coffee Leaves (Coffea liberica L.) with Addition Rhizosphere Bacterial Microcapsules from Eruption Soil of Mount Sinabung

Authors

Kabul Warsito , Desi Sri Pasca Sari Sembiring , Indra Irawan , Bagus Kriswanda

DOI:

10.29303/jbt.v25i4.10221

Published:

2025-10-22

Issue:

Vol. 25 No. 4 (2025): in Progress

Keywords:

Liberica coffee, Microcapsules, Rhizosphere bacteria

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Warsito, K., Sembiring, D. S. P. S., Irawan, I., & Kriswanda, B. (2025). Growth Rate and Chlorophyll Content of Liberica Coffee Leaves (Coffea liberica L.) with Addition Rhizosphere Bacterial Microcapsules from Eruption Soil of Mount Sinabung. Jurnal Biologi Tropis, 25(4), 5873–5880. https://doi.org/10.29303/jbt.v25i4.10221

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Abstract

The chlorophyll content in coffee leaves greatly influences growth productivity and productivity. This study aimed to examine the effect of seed soaking and rhizosphere bacteria microcapsule dosage on the growth rate and chlorophyll content of Liberica coffee leaves planted on peatland. The experiment used a completely randomized design (CRD) with two factors, namely seed soaking time (0, 9, 10, and 11 hours) and rhizosphere bacteria microcapsule dosage (0, 10, 15, and 20 grams), with a total of 16 treatment combinations replicated twice. The parameters measured included leaf area, number of leaves, and chlorophyll content. The results showed that 11-hour seed soaking treatment produced the largest leaf area (40.32 mm), 9–10 hours soaking gave the highest number of leaves (10.34), and 20 grams of microcapsules gave the highest chlorophyll content (79.16 CCI). ANOVA and Duncan's test showed that seed soaking and microcapsules significantly affected the growth and chlorophyll content of leaves. In conclusion, the combination of seed soaking for 9–11 hours and a 20 gram microcapsule dose can improve the growth and quality of Liberica coffee leaves.

References

Agustiyani, D., Dewi, T. K., Laili, N., Nditasari, A., & Antonius, S. (2021). Exploring biofertilizer potential of plant growth-promoting rhizobacteria candidates from different plant ecosystems. Biodiversitas, 22(5), 2691–2698. https://doi.org/10.13057/biodiv/d220529

Dharmadewi, I. M. (2020). Analisis Kandungan Klorofil Pada Beberapa Jenis Sayuran HijauSebagai Alternatif Bahan Dasar Food Suplement. Jurnal Emasains, 9(2), 171–177. https://doi.org/10.5281/zenodo.4299383

Fang, Z., & Bhandari, B. (2022). Encapsulation of bioactive compounds for food and agricultural applications. Molecules, 27(19), 6413. https://doi.org/10.3390/molecules27196413

Fatimah, S., & Titisari, P. W. (2024). Pemberian POC Limbah Kulit Kopi dan Kitosan Terhadap Pre-Nursery Kopi Liberika (Coffea liberica Var. Liberica). Jurnal Ilmiah Pertanian (JIPERTA), 6(2), 109-123. https://doi.org/10.31289/jiperta.v6i2.4270.

Fauziah, N. O., Rusdiono, N. N., Simarmata, T., & Fitriatin, B. N. (2022). Media Produksi Massal Pupuk Hayati Penambat N Azotobacter sp. dari Rizosfer di Indonesia Mass Production Media of Biofertilizer N Fixer Azotobacter sp. from Rhizosphere in Indonesia. Terakreditasi RISTEKBRIN Peringkat SINTA, 18(1), 2020. https://doi.org/10.30598/jbdp.2022.18.1.13

Glick, B. R. (2012). Plant growth-promoting bacteria: Mechanisms and applications. Scientifica, 2012, 963401. https://doi.org/10.6064/2012/963401

Handayani, A., Sutariati, G. A. K., Nurmas, A., & Sadimantara, G. R. (2024). Efektivitas Biopriming Mikroba Indigenos Asal Rhizosfer Tanaman Kopi terhadap Viabilitas dan Vigor Benih Kopi (Coffea robusta L.) di Kabupaten Buton. Berkala Penelitian Agronomi, 12(2), 82-92. DOI: https://doi.org/10.33772/bpa.v12i2.1415.

Haniefan, N., & Basunanda, P. (2022). Eksplorasi dan Identifikasi Tanaman Kopi Liberika di Kecamatan Sukorejo, Kabupaten Kendal. Vegetalika, 11(1), 11–18. https://doi.org/10.22146/veg.44325

Kaur, R., Kaur, S., Dwibedi, V., Kaur, C., Akhtar, N., & Alzahrani, A. (2023). Development and characterization of rice bran-gum Arabic based encapsulated biofertilizer for enhanced shelf life and controlled bacterial release. Frontiers in Microbiology, 14, 1267730. https://doi.org/10.3389/fmicb.2023.1267730.

Kazeem, M. O., Sorunke, T. A., Ahmed-El-Imam, A., & Zainudin, M. H. M. (2024). Impact of decomposing sawdust as an inoculum for promoting the composting of sawdust and chicken manure. International Journal of Recycling of Organic Waste in Agriculture, 13. https://doi.org/10.57647/ijrowa-ty46-8z37.

Ma, Z., Wang, N., He, H., & Tang, X. (2016). Encapsulation and characterization of slow-release nitrogen fertilizer based on superabsorbent polymer. Reactive and Functional Polymers, 99, 87-94. https://doi.org/10.1016/j.reactfunctpolym.2015.12.017

Rohman, F. A., & Taufika, R. (2024). Pengaruh Perendaman Zat Pengatur Tumbuh Giberelin (GA3) pada Perkecambahan Benih Kopi Arabika (Coffea arabica L.) Varietas S795. Jurnal Agro Industri Perkebunan, 11-18. https://doi.org/10.25181/jaip.v12i1.3327.

Marcial‐Coba, M. S., Soria‐Delgado, L., & Yánez‐Altuna, J. M. (2018). Encapsulation Technologies of Plant Growth‐Promoting Microorganisms and Microbial Biological Control Agents Against Plant Pathogenic Organisms. Annual Plant Reviews online, 397-436. https://doi.org/10.1002/9781119312994.apr0731.

Maxiselly, Y., Nafy, F. R., & Anjarsari, I. R. D. (2023). Morphological trait variation of the immature liberica coffee (Coffea liberica) from West Java, Indonesia applied difference of coffee husk compost and biofertilizer. Biodiversitas, 24(11), 5988–5994. https://doi.org/10.13057/biodiv/d241118

Nasrullah, N., Ibrahim, B., & Robbo, A. (2023). Pengaruh Pemberian Berbagai Macam Pupuk Organik Padat Terhadap Kemampuan Tanah Menyimpan Air. AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian, 4(2), 200–205. https://doi.org/10.33096/agrotekmas.v4i2.336

Panichikkal, J., Prathap, G., Nair, R. A., & Krishnankutty, R. E. (2021). Evaluation of plant probiotic performance of Pseudomonas sp. encapsulated in alginate supplemented with salicylic acid and zinc oxide nanoparticles. International Journal of Biological Macromolecules, 166, 138–143. https://doi.org/https://doi.org/10.1016/j.ijbiomac.2020.10.110

Park, S., Kim, A. L., Hong, Y. K., Shin, J. H., & Joo, S. H. (2021). A highly efficient auxin-producing bacterial strain and its effect on plant growth. Journal, genetic engineering & biotechnology, 19(1), 179. https://doi.org/10.1186/s43141-021-00252-w

Pôrto, M. L., Alves, J. C., Souza, A. P., Araújo, R. C., & Arruda, J. A. (2014). Chlorophyll as a measure of plant health: Agroecological aspects. Pesticidi i Fitomedicina, 29(1), 21-34. https://doi.org/10.2298/PIF1401021P.

Purba, I. G., Warsito, K., & Refnizuida, R. (2023). Escalation Of Coffee Plant (Coffea arabica L) By Addition Of Microcapsules From IAA (Indole Acetic Acid) Producing-Endophytic Bacteria. Jurnal Pembelajaran dan Biologi Nukleus, 9(1), 181-191. https://doi.org/10.36987/jpbn.v9i1.3907.

Rasyidi, A. F., Sulistiani, R., & Bin, I. (2024). Kadar Klorofil Daun Bibit Kelor (Moringa oleifera L.) pada Berbagai Dosis Kompos. AGRIUM: Jurnal Ilmu Pertanian, 27(1). https://doi.org/10.30596/agrium.v27i1.17486

Riyani, K., Widyaningsih, S., Nugroho, A. A., & Priyanto, G. (2020). Karakteristik dan aktivitas antioksidan kopi liberika. Jurnal Kimia Sains dan Aplikasi, 23(5), 166-171. https://doi.org/10.14710/jksa.23.5.166-171

Rodriguez, H., Gonzalez, T., Goire, I., & Bashan, Y. (2004). Gluconic acid production and phosphate solubilization by the plant growth-promoting bacterium Azospirillum spp. Naturwissenschaften, 91(11), 552-555. https://doi.org/10.1007/s00114-004-0567-8

Rokhmah, D. N., Dani, D., Sakiroh, S., Pranowo, D., & Sasmita, K. D. (2023). Pertumbuhan dan perkembangan tanaman kopi liberika (Coffea liberica) belum menghasilkan pada beberapa jenis pohon penaung. Jurnal AGRO, 10(2), 231–241. https://doi.org/10.15575/25202

Sabrina, T. (2021). Diversity of non-symbiotic nitrogen-fixing bacteria and their potential in andisols affected by the eruption of Mount Sinabung, North Sumatra, Indonesia. Biodiversitas Journal of Biological Diversity, 22(8). https://doi.org/10.13057/biodiv/d220854.

Suriani, N. L., Al-Zharani, M., Nasr, F. A., Alneghery, L. M., Suprapta, D. N., Suarsana, I. N., Resiani, N. M. D., Enshasy, H. A. E., Sayyed, R., Barasarathi, J., Ho, T. S., & Yanti, Y. (2025). Rhizobacteria consortium improves growth, yield, and phytochemicals in Robusta coffee (Coffea canephora L.). Frontiers in microbiology, 16, 1602940. https://doi.org/10.3389/fmicb.2025.1602940

Yulianti, M., S, S., & Buhaira, B. (2022). Respons Pertumbuhan Bibit Kopi Liberika (Coffea liberic W. Bull Ex Hiern) Terhadap Aplikasi Pupuk Kandang Sapi Di Polybag. Jurnal Agroecotania : Publikasi Nasional Ilmu Budidaya Pertanian, 5(2), 23–34. https://doi.org/10.22437/agroecotania.v5i2.23037

Warsito, K., Hafiz, M., Irawan, I., & Friski, F. I. (2024). Effectiveness of rhizosphere bacteria microcapsules on the growth of arabica coffee leaves (Coffea arabica L.). In Proceedings of the 2nd Annual Dharmawangsa International Conference: Digital Technology and Environmental Awareness in Promoting Sustainable Behavior in Society 5.0 (pp. 402-411). Dharmawangsa University. DOI: https://doi.org/10.46576/prosftik.v1i1.435.

Warsito, K., Hafiz, M., Irawan, I., & Friski, F. I. (2024). Potential of rhizosphere bacteria microcapsules isolated from Sinabung volcano in stimulating arabica coffee growth (Coffea arabica L.). International Journal of Research and Review, 11(11), 481-488. https://doi.org/10.52403/ijrr.20241148

Yadav, A. N., Verma, P., Kumar, S., Kumar, V., Kumar, M., Sugitha, T. C. K., Singh, B. P., Saxena, A. K., & Dhaliwal, H. S. (2021). Biofertilizers: An ecofriendly technology for nutrient recycling and environmental sustainability. Current Research in Microbial Sciences, 2, 100026. https://doi.org/10.1016/j.crmicr.2021.100026

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Growth Rate and Chlorophyll Content of Liberica Coffee Leaves (Coffea liberica L.) with Addition Rhizosphere Bacterial Microcapsules from Eruption Soil of Mount Sinabung

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