Oxidation Parameters of Sardine Oil Extracted from Fish Oil Emulsion
Authors
Kristina HaryatiDOI:
10.29303/jbt.v25i4b.11084Published:
2025-12-26Issue:
Vol. 25 No. 4b (2025): Special IssueKeywords:
Bligh and dryer extraction method, Emulsion, Fish oil, Oxidation parametersArticles
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Abstract
Fish oil contains polyunsaturated fatty acids, so it is made into an emulsion preparation. The emulsifier used must be able to protect the oil during storage. The purpose of this study was to determine the oxidation parameters of sardine fish oil extracted from the emulsion. The research method was divided into 2 stages, namely oil extraction from the emulsion using the Bligh and Bryer methods; oxidation parameter testing carried out included determining the levels of free fatty acids (FFA), peroxide value (PV), acid value (AV), anisidine value (AnV), and total oxidation (Totoks) based on AOCS 1998. The results showed that the highest FFA value was in formula 3:2 followed by formula 1:2, 1:1, and the lowest was in formula 3:1. The highest PV value was in formula 1:2 followed by formula 1:1, 3:2, and the lowest was in formula 3:1. The highest AV value was in formula 3:2 followed by formula 1:2, 1:1, and the lowest was in formula 3:1. The highest AnV and Totoks values were found in formula 1:2 followed by formula 1:1, 3:2, and the lowest was in formula 3:1. These findings indicate that the oil extracted from formula 3:1 has lower oxidation parameter values than the others. Thus, this study indicates that the oxidation parameters of the oil extracted from the emulsion have not met the IFOS standard, and another emulsifier is needed to protect the oil in the form of an emulsion preparation during storage.
References
Apituley, D. A. N., Bonan, R., Sormin, D., & Nanlohy, E. E. E. M. (2020). Karakteristik dan Profil Asam Lemak Minyak Ikan dari Kepala dan Tulang Ikan Tuna (Thunnus albacares). AGRITEKNO: Jurnal Teknologi Pertanian, 9(1): 10-19. https://doi.org/10.30598/jagritekno.2020.9.1.10.
Cahyono, H., Yulianto, A., Assyfa, N., Fabella, N., & Suseno, S.H. (2022). Kualitas Minyak Ikan dari Hasil Samping, dan Produk (softgel) Minyak Ikan Komersial. Jurnal Harpodon Borneo, 15(2). https://doi.org/10.35334/harpodon.v15i2.2125.
Evanuarini, H., Nurliyani, N., Indratiningsih, I., & Hastuti, P. (2019). Kestabilan Emulsi dan Oksidasi Low Fat Mayonnaise Menggunakan Kefir sebagai Alternatif Emulsifier . Jurnal Ilmu-Ilmu Peternakan, 29(1): 83-94. https://doi.org/10.21776/ub.jiip.2019.029.01.10.
Haetami, K., Abun, A., Putri, A. D., Willyanissa, F. F., Aldiansyah, A., Sihombing, M. K. T. (2025). Quantitative Analysis of Omega-3 Content in Fish Waste and Its Application as Aquafeed. Jurnal Biologi Tropis, 25(2): 2743-2750. https://doi.org/10.29303/jbt.v25i3.930.
Khoirunnisa, Z., Wardana, A. S., & Rauf, R. (2019). Angka Asam dan Peroksida Minyak Jelantah dari Penggorengan Lele Secara Berulang. Jurnal Kesehatan, 12(2): 81–90. https://doi.org/10.23917/jk.v12i2.9764.
Lika, L. C. R., Luhtansa, S. S., Blaon, S. B., & Panjaitan, R. S. (2022). Perbandingan Bilangan Asam pada Sampel Minyak Goreng Kemasan dan Curah. Indonesian Journal of Pharmaceutical Research, 2(2): 22-26.
Liputo, S. A., Une, S., Mutsyahidan, A. M. A., Lodi, S., Ibrahim, N., Tunai, K., Malabali, A. P., & Sari, N. P. (2024). Pengaruh Suhu Penyimpanan Terhadap Minyak Kelapa Tradisional.. Prosiding Seminar Nasional Mini Riset Mahasiswa, 3(1): 72-78.
Liputo, S.A., Une, S., Mutsyahidan, A.M.A., Lodi, S., Ibrahim, N., Tunai, K., Malabali, A.P., & Sari, N.P. (2024). Pengaruh Suhu Penyimpanan Terhadap Minyak Kelapa Tradisional.. Prosiding Seminar Nasional Mini Riset Mahasiswa, 3(1): 72-78.
Musbah, M., Am, R. F., Adel, Y. S., & Muliadin, M. (2018). Emulsi Kaya Omega-3 dan Squalen dari Kombinasi Minyak Ikan Sardin dan Cucut. Jurnal Pengolahan Perikanan,. 3(1): 16-21. https://doi.org/10.31970/pangan.v3i1.8.
Mutsyahidan, A. M. A., Liputo, S. A., Une, S., Isima, R., Podungge, F. F., Nurdin, D., Djama, A., & Nurdin, A. (2024). Pengaruh Suhu Penyimpanan Terhadap Mayonnaise Low-Kolesterol Berbahan Dasar Minyak Kanola (CO) Dengan Virgin Coconut Oil (VCO). Prosiding Smeinar Nasional Mini Riset Mahasiswa, 3(1): 85-95.
Octavia, W., Putri, S. K., & Wulansari, A. (2023). Analisis Kimia Minyak Goreng Bekas Pedagang Gorengan Dengan Metode Deep Frying di Lingkungan Perguruan Tinggi Swasta Kota Jambi. Jurnal Diskursus Ilmiah Kesehatan, 1(2): 79-87. https://doi.org/10.56303/jdik.v1i2.161
Pandiangan, M., Daniela, C., Sihombing, D. R., & Daeli, W. K. (2023). Komposisi Kandungan Asam Lemak pada Minyak Ikan Sarden (Sardinella sp.). Jurnal Riset Teknologi Pangan Dan Hasil Pertanian (RETIPA), 4(1): 1-7. https://doi.org/10.54367/retipa.vi.3170.
Pratiwi, N. P. C., & Meikapasa, N. W. P. (2025). Analisis Total Oxidation Value Minyak Goreng Bekas pada Beberapa Produk Olahan Pangan Di Pasaran. Jurnal Teknologi dan Mutu Pangan, 3(2): 88-95. https://doi.org/10.30812/jtmp.v3i2.4782.
Purwaningsih, I. (2012). Perbandingan Kadar Bilangan Asam Minyak Goreng Sawit Curah Yang Ditambahkan Ekstrak Wortel Dengan Yang Tidak. Jurnal Vokasi Kesehatan, 1(2): 59-63. https://doi.org/10.30602/jvk.v1i2.15.
Rantawi, A. B. (2017). Korelasi Kadar Air Pada Kernel Terhadap Mutu Kadar Asam Lemak Bebas Produk Palm Kernel Oil Yang Dihasilkan. Politeknik Kelapa Sawit Citra Widya Edukasi, 13(1): 27–32. https://doi.org/10.53912/iejm.v6i1.156.
Rizkyyani, P., Khusna, A., Hilmi, M., Khirzin, M. H., & Triasih, D. (2020). Pengaruh Lama Penyimpanan Dengan Berbagai Bahan Penstabil Terhadap Kualitas Mayonnaise. Jurnal Ilmu dan Teknologi Peternakan Tropis, 7(1): 52-58. http://dx.doi.org/10.33772/jitro.v7i1.8381.
Rozi, A., Suseno, S. H., & Jacoeb, A. M. (2016). Ekstraksi dan Karakterisasi Minyak Hati Cucut Pisang. JPHPI, 19(2): 100-109. https://doi.org/10.17844/jphpi.2016.19.2.100.
Setyopratiwi, A., Titiek, H., & Hanifah, U. (2022). Formulasi Dan Stabilitas Mikroemulsi Minyak Dalam Air Dengan Virgin Coconut Oil ( VCO ) sebagai Fase Minyak Menggunakan Metode Emulsifikasi. Prosiding Seminar Nasional Kimia, 108-123.
Sitorus, S., Bagus, I., Parta, B., & Ruswanto, A. (2022). Pembuatan Margarin Dari Lemak Cokelat Yang Disubstitusi Dengan Minyak Sawit Merah. JBFT, 1(2): 113–123. https://doi.org/10.55180/biofoodtech.v1i02.279.
Suseno, S. H., Jacoeb, A. M., & Abdulatip, D. (2019). Stabilitas Minyak Ikan Komersial (Soft Gel) Impor Di Beberapa Wilayah Jawa Timur. JPHPI, 22(3): 589-600. https://doi.org/10.17844/jphpi.v22i3.29229.
Suseno, S. H., & Listiana, D. (2020). Ekstraksi Wet Rendering Minyak Ikan Layang (Decapterus macarellus) Dengan Suhu Rendah. JPHPI, 23(3): 495-502. https://doi.org/10.17844/jphpi.v23i3.32629.
Telaumbanua, M. C., & Utama, Q. D. (2024). Pengaruh Lama Pemanasan Pada Penurunan Kualitas Mutu Minyak Goreng Sawit Komersil. Jurnal Teknologi dan Mutu Pangan, 2(2): 117-125. https://doi.org/10.30812/jtmp.v2i2.3751.
Tran, T., Tsekeridis, C., Hasyyati, F. I., Yesiltas, B., Mendes, A. C., Chronakis, I. S. C., Berton-Carabin, C., Bordallo, H. N., Schroën, K., & Jacobsen, C. (2025). Lipid Oxidation of Fish Oil-in-Water Pickering Emulsions Stabilized With Protein-Based Janus Particles. European Journal Of Lipid Science and Technology, 1-13. https://doi.org/10.1002/ejlt.70065
Triyanti, S. B., Lestari, F. P., Anisa, P., Fitriana, N., Rostiana, H. R., Silalahi, D. D., Syalsabina, T. D., Putri, R. Y., & Saputra, I. S. (2025). Pengaruh Metode Ekstraksi Maserasi , Sonikasi , Dan Sokletasi Terhadap Nilai Rendemen Sampel Kulit Buah Naga (Hylocereus polyrhizus). Jurnal Sains dan Edukasi Sains, 8(1): 71-78. https://doi.org/10.24246/juses.v8i1p71-78.
Untari, B., Miksusanti, & Ainna, A. (2020). Penentuan Kadar Asam Lemak Bebas Dan Kandungan Jenis Asam Lemak Dalam Minyak Yang Dipanaskan Dengan Metode Titrasi Asam Basa Dan Kromatografi Gas. Jurnal Ilmiah Bakti Farmasi, 1(1): 1-10. https://ejournal.stifibp.ac.id/index.php/jibf/article/view/58.
Utama, Q. D., Sitanggang, A. B., Adawiyah, D. R., & Hariyadi, P. (2020). Lipase-Catalyzed Synthesis Of Medium-Long-Medium-Type Of Structured Lipids From Refined Bleached Deodorized Olein. Applied Food Biotechnology, 7(2): 85-94. https://doi.org/10.22037/afb.v7i2.26807.
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