Development of Curcuma xanthorrhiza Nanoemulsion as an Antibiofilm Agent against Multidrug-Resistant Candida albicans: Formulation Approach and In Vitro Evaluation

Dzulfira Arifah; Nurul Izzah; Dyah Virga Pujiani; Abidah Ardilia; Masfufatun Masfufatun

doi:10.29303/jbt.v26i1.11274

Development of Curcuma xanthorrhiza Nanoemulsion as an Antibiofilm Agent against Multidrug-Resistant Candida albicans: Formulation Approach and In Vitro Evaluation

Authors

Dzulfira Arifah , Nurul Izzah , Dyah Virga Pujiani , Abidah Ardilia , Masfufatun Masfufatun

DOI:

10.29303/jbt.v26i1.11274

Published:

2026-02-08

Issue:

Vol. 26 No. 1 (2026): Januari-Maret

Keywords:

Antibioiflm, Candida albicans, Nanoemulsion, Temulawak

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Arifah, D., Izzah, N., Pujiani, D. V., Ardilia, A., & Masfufatun, M. (2026). Development of Curcuma xanthorrhiza Nanoemulsion as an Antibiofilm Agent against Multidrug-Resistant Candida albicans: Formulation Approach and In Vitro Evaluation. Jurnal Biologi Tropis, 26(1), 605–613. https://doi.org/10.29303/jbt.v26i1.11274

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Abstract

Candida albicans infection is a serious health concern, particularly in immunocompromised individuals, due to its ability to form biofilms that are resistant to conventional antifungal therapies. Curcuma xanthorrhiza Roxb. (temulawak) possesses antifungal properties; however, its effectiveness is limited by poor solubility and bioavailability. Nanoemulsion formulation offers a promising strategy to enhance the penetration of active compounds. This study aimed to develop and evaluate the effectiveness of temulawak nanoemulsion as an antibiofilm agent against multidrug-resistant (MDR) C. albicans. Using a post-test control group only design, the antibiofilm assay was conducted via the microtiter plate method and analyzed based on Optical Density (OD) measurements. The results showed that temulawak nanoemulsion significantly reduced biofilm formation, with an 89% reduction in OD at the 100% concentration. These findings support the potential use of temulawak nanoemulsion as an alternative therapeutic strategy for Candida albicans infections, particularly those involving drug-resistant biofilms.

References

Asita, N., & Zubair, M. S. (2023). Formulasi Self-Nanoemulsifying Drug Delivery System (SNEDDS) yang Memanfaatkan Tanaman Obat: Narrative Review. Jurnal Sains Farmasi & Klinis, 10(2), p.184. https://doi.org/10.25077/jsfk.10.2.184-196.2023 .

Atun, S., Zoulina, S. D., Izzana, A. Y., Aznam, N., & Sangal, A. (2022). Effect of curcuminoid nanoemulsion extracted from Curcuma Xanthorrhiza ON HYPERCHOLESTEROLEMIC RATS. Rasayan Journal of Chemistry, 53. https://doi.org/10.31788/RJC.2022.1558096

Chen, Q., Xiong, S., Ye, T., Gao, Y., Wang, J., Li, X., ... & Zheng, J. (2024). Insulin resistance, coronary artery lesion complexity and adverse cardiovascular outcomes in patients with acute coronary syndrome. Cardiovascular Diabetology, 23(1), 172. https://doi.org/10.1186/s12933-024-02276-1

Cho, M. Y., Lee, E. S., Jung, H. I., & Kim, B. I. (2023). Anti-biofilm activity of a novel nanoemulsion containing Curcuma xanthorrhiza oil. Journal of Dentistry, 137, 104647. https://doi.org/10.1016/j.jdent.2023.104647

Dewi, A.P., (2025). Potensi Ekstrak Temulawak (Curcuma xanthorrhiza Roxb) Terhadap Jamur Candida albicans Penyebab Keputihan. JFARM - Jurnal Farmasi, 3(2), pp.65–73. https://doi.org/10.58794/jfarm.v3i2.1532

Fitriani, H., Fitria, A., Annisa, I., & Syukri, Y. (2021). Pengembangan Self-Nano Emulsifying System (SNES) Ekstrak Temulawak (Curcuma xanthorrhiza): Formulasi, Karakterisasi, dan Stabilitas. Jurnal Sains Farmasi & Klinis, 8(3), p.332. https://doi.org/10.25077/jsfk.8.3.332-339.2021

Hajrin, W., Subaidah, W. A., & Juliantoni, Y. (2024). Formulation And Charachterization of Nanoemulsion from Brucea javanica Seed Extract. Indones J Pharm Sci Technol J Homepage, 11(1), 117-125. https://doi.org/10.24198/ijpst.v11i1.38691

H Hamzah, H., Gunawan, D. R., Pratiwi, S. U. T., Bakhtiar, M. I., Pratama, V. Y., Subhan, M., & Maulana, R. (2024). Nanoemulsion Mouthwash Formulation of Bajakah Tampala (Spatholobus littoralis Hassk.) Skin Extract Against Candida albicans. Borneo Journal of Pharmacy, 7(1), 1-13. https://doi.org/10.33084/bjop.v7i1.5548.

Harimurti, N., Nasikin, M., & Mulia, K. (2021). Water-in-oil-in-water nanoemulsions containing temulawak (Curcuma xanthorriza Roxb) and red dragon fruit (Hylocereus polyrhizus) extracts. Molecules, 26(1), 196. https://doi.org/10.3390/molecules26010196.

Kaur, J., & Nobile, C. J. (2023). Antifungal drug-resistance mechanisms in Candida biofilms. Current opinion in microbiology, 71, 102237. .https://doi.org/10.1016/j.mib.2022.102237

Kim, H.I., Huang, J.H., Guo, M., Park, J.H. & Cho, B.H. (2019). Antifungal activity of Curcuma xanthorrhiza essential oil against Candida albicans opportunistic pathogen. Sphere, 4(3). https://doi.org/10.1002/ptr.4834

Kumar, L., Bisen, M., Harjai, K., Chhibber, S., Azizov, S., Lalhlenmawia, H., & Kumar, D. (2023). Advances in nanotechnology for biofilm inhibition. ACS omega, 8(24), 21391-21409. https://doi.org/10.1021/acsomega.3c02239.

Lithamivitra, L., Aeni, N., Miftah, N., Kardinal, K., Reza, A., Isdaryanti, I., ... & Nurdin, G. M. (2024). Formulaton of nanoemulsion biopesticide for horticultural plants from fermented extracts of jongi plant and cigarette butts. Jurnal Biologi Tropis, 24(4), 568-573. https://doi.org/10.29303/jbt.v24i4.7509 .

Marisa, Y.T. & Mulyana, R. (2020). Infeksi Jamur Pada Geriatri. Human Care Journal, 5(1), p.328. https://doi.org/10.32883/hcj.v5i1.615 .

Masfufatun, Z.F., Agustin Angraeni, D.P. and Nuzula, F., 2024. Efek Sinergis Vitamin C Dalam Meningkatkan Kinerja Antijamur Madu Apis dorsata Terhadap Biofilm Candida albicans Untuk Terapi Kandidiasis. Hang Tuah Medical Journal, 22(1), pp.1–14. https://doi.org/10.30649/htmj.v22i1.698 .

Pitaloka, D.A.E., Chiuman, L., Ginting, C.N. & Lister, I.N.E. (2024). Antioxidant and Antifungal Activity of Temulawak (Curcuma xanthorrhiza Roxb.) Extract against Candida albicans. Jurnal Sains Kesehatan, 6(1), pp.101–110. https://doi.org/10.25026/jsk.v6i1

Prawita, A. & Nurfadjriah, N. (2025). Aktivitas Antijamur Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza Roxb) Terhadap Pertumbuhan Jamur Candida albicans Secara In Vitro. Intellektika: Jurnal Ilmiah Mahasiswa dan Akademisi, 3(4), pp.307–216. https://doi.org/10.58794/jfarm.v3i2.1532

Putri, R.A. & Masfufatun. (2022). Karakteristik Biofilm Candida Albicans dan Beberapa Antibiofilmnya. Medika Kartika Jurnal Kedokteran dan Kesehatan, (Volume 5 No 2), pp.208–219. https://doi.org/10.35990/mk.v5n2.p208-219

Rahmat, E., Lee, J., & Kang, Y. (2021). Javanese turmeric (Curcuma xanthorrhiza Roxb.): Ethnobotany, phytochemistry, biotechnology, and pharmacological activities. Evidence‐Based Complementary and Alternative Medicine, 2021(1), 9960813. https://doi.org/10.1155/2021/9960813 .

Singh, M., Rehman, A., Hassan, N., Anfey Faheem, A., Das, A., Rahman, M., ... & Iqbal, Z. (2023). Exploration of a W/O nanoemulsion for antibiofilm activity against cariogenic Enterococcus faecalis. ACS omega, 8(3), 2871-2879. https://doi.org/10.1021/acsomega.2c03180 .

Soegiharto, B. C., Pratiwi, R., & Wardhana, E. S. (2021). Efektivitas nanoemulsi gel daun pegagan (centella asiatica l.) konsentrasi 25%, 50% dan 75% terhadap ketebalan biofilm bakteri staphylococcus aureus (in vitro). Prosiding Konstelasi Ilmiah Mahasiswa Unissula (KIMU) Klaster Kesehatan. https://jurnal.unissula.ac.id/index.php/kimukes/article/view/20283/6487

Thomas-Rüddel, D. O., Schlattmann, P., Pletz, M., Kurzai, O., & Bloos, F. (2022). Risk factors for invasive Candida infection in critically ill patients: a systematic review and meta-analysis. Chest, 161(2), 345-355. https://doi.org/10.1016/j.chest.2021.08.081.

Vikri, M., Sholih, M. G., & Gatera, V. A. (2022). Identifikasi Kadar Kurkumin pada Minuman Serbuk Berbahan Temulawak dengan Metode Spektrofotometri Uv-Vis. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 3(2), 191-196. https://doi.org/10.31764/lf.v3i2.9303

Warmasari, N. W. M., Ernawati, D. K., Indrayani, A. W., Dewi, N. W. S., & Jawi, I. M. (2020). Antibacterial activity from temulawak extract (Curcuma xanthorrhiza Roxb) on growth inhibition of Staphylococcus epidermidis in vitro. Jurnal Epidemiologi Kesehatan Komunitas, 5(1), 1-7. https://doi.org/10.14710/jekk.v5i1.6909 .

Yasacaxena, L.N., Defi, M.N., Kandari, V.P., Weru, P.T.R., Papilaya, F.E., Oktafera, M. & Setyaningsih, D., (2023). Review: Extraction of Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) and Activity As Antibacterial. Jurnal Jamu Indonesia, 8(1), pp.10–17. https://doi.org/10.29244/jji.v8i1.265 .

Yulianti, O. & Harefa, Y.S. (2021). Pengolahan Temulawak Plant (Curcuma xanthorrhiza Roxb) As A Tradisional Medicine. Journal Health Of Education, 2(2). https://doi.org/10.62611/jhe.v4i2.380

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Development of Curcuma xanthorrhiza Nanoemulsion as an Antibiofilm Agent against Multidrug-Resistant Candida albicans: Formulation Approach and In Vitro Evaluation

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