Study of the Difference in Biodegradation Rate of LAS (Linear Alkylbenzene Sulfonates) and MES (Methyl Ester Sulfonate) Surfactants by Pseudomonas putida and Bacillus megaterium
DOI:
10.29303/jbt.v26i1.11387Published:
2026-01-19Downloads
Abstract
Linear Alkylbenzene Sulfonate (LAS) is the most widely used surfactant in industrial detergent formulations, generating waste that poses potential risks to environmental quality and human health. As an environmentally friendly alternative, palm oil–based Methyl Ester Sulfonate (MES) has renewable and biodegradable properties, stability in hard water and high salinity, and a faster degradation rate than LAS. However, direct comparative studies on the biodegradation rates of LAS and MES are still limited. Therefore, this study aimed to compare the biodegradation ability of Pseudomonas putida and Bacillus megaterium toward LAS and MES in vitro using the MBAS method. The experiment employed a Completely Randomized Design with a two-factor factorial arrangement and three replications, resulting in 12 experimental units. Biodegradation rates were determined by measuring surfactant concentrations at days 0, 2, 4, 6, and 8 using UV-Vis spectrophotometry and linear regression analysis. The results showed a decrease in surfactant concentration over time in all treatments. Statistical analysis indicated no significant difference between LAS and MES biodegradation rates, but significant differences between bacterial species and their interaction with surfactant type.
Keywords:
Biodegradation Detergents Surfactants WasteReferences
Abd Maurad, Z., Idris, Z., & Ghazali, R. (2017). Performance of palm-based C16/18 methyl ester sulphonate (MES) in liquid detergent formulation. Journal of Oleo Science, 66(7), 677-687. DOI.https://doi.org/10.5650/jos.ess16190
Afida, S., Razmah, G., & Zulina, A. M. (2016). Biodegradation of various homologues of palm-based methyl ester sulphonates (MES). Sains Malaysiana, 45(6), 949-954. DOI. https://www.researchgate.net/publication/306233769
Alnuaimi, M. T., Taher, T. A., Aljanabi, Z. Z., & Adel, M. M. (2020). High-resolution GC/MS study of biodegradation of crude oil by Bacillus megaterium. Research on Crops, 21(3), 650-657. DOI. 10.31830/2348-7542.2020.101.
Amelia, S., Jamilatun, S., Shitopyta, L. M., Maryudi, M., Utami, M., & Sriyana, I. (2023). Degradasi Limbah Detergen dengan Metode Fotokatalis Menggunakan TiO2/Silica Gel. Eksergi, 20(3), 131-136.
Badmus, S. O., Amusa, H. K., Oyehan, T. A., & Saleh, T. A. (2021). Environmental risks and toxicity of surfactants: overview of analysis, assessment, and remediation techniques. Environmental Science and Pollution Research, 28(44), 62085-62104.
Burns & Jelen (2009). Anaerobic biodegradation of detergent surfactants. Materials, 2(1), 181-206.
Bustami, Abdullah D., Fadlisyah. 20014. Statistika: Terapannya pada Bidang Informatika. Yogykarta: Graha Ilmu.
Dhani, P. R. R., Hendrayana, H., Hidayati, N. V., & Haryati, A. (2023). Konsentrasi Limbah Pencemar MBAS (Methylen Blue Active Substance) pada Perairan Muara Kali Ijo, Kabupaten Kebumen. Maiyah. 2(3), 181-190. DOI:10.20884/1.maiyah.2023.2.3.9695
Dhanya, V. (2019). A statistical tool for the optimization of parameters for the degradation of mono-aromatic pollutants by a formulated microbial consortium. Journal of Pure and Applied Microbiology, 13(4), 2251-2260. DOI. https://doi.org/10.22207/JPAM.13.4.39
Dwicania, E. (2019). Biodegradasi Limbah Plastik Oleh Mikroorganisme. URL: https://osf.io/yjzu8
Eniola, K. I. T. (2011). Effect of additional carbon source on biodegradation of linear alkylbenzene sulfonate by las-utilizing bacteria. Journal of Xenobiotics, 1(1), e2. https://doi.org/10.4081/xeno.2011.e2.
Farn, R. J. (2007). Chemistry and technology of surfactants. Blackwell Publishing Ltd.
Frontier Research. (2024). Frontier Research – Marketing research based in Indonesia. Frontier. https://frontier.co.id/business/frontier-research/
Kosswig, K. (2009). Surfactants. In M. Bohnet, C. J. Brinker, H. Clemens, B. Cornils, T. J. Evans, H. Greim, L. L. Hegedus, J. Heitbaum, W. A. Herrmann, U. Karst, et al. (Eds.), Ullmann’s encyclopedia of industrial chemicals. Wiley-VCH Verlag GmbH.
Naufal, B. S., & A’yun, D. Q. (2024). Analisis dampak pencemaran tanah akibat limbah deterjen terhadap lingkungan hidup masyarakat di daerah pedesaan. Student Research Journal, 2(3), 231-235. DOI:10.55606/srjyappi.v2i3.1320
Olivera, E.,R., Carnicero, D., GarcõÂa, B., MinÄambres, B., A. Miguel, Moreno, CanÄedo,L., DiRusso, C., Naharro, G., dan, Luengo, J. 2001. Two different pathways are involved in the b-oxidation of n-alkanoic and n-phenylalkanoic acids in Pseudomonas putida U: genetic studies and biotechnological applications. Molecular Microbiology. 39(4), 863-874. https://doi.org/10.1046/j.1365-2958.2001.02296.x
Putri, Eka, P. (2024). Penentuan Kadar Surfaktan pada Sampel Air Permukaan dengan Metode MBAS (Methylene Blue Active Substances) menggunakan Spektrofotometer UV-Vis. Jurnal Pendidikan Tambusai. 8(2). Hal: 27899-27906. DOI. 10.31004/jptam
Sabli, T. E., & Zahrah, S. (2015). Reduksi kandungan fosfat dalam air limbah deterjen menggunakan sistem rawa bambu. Dinamika Pertanian, 30(2), 101-108. https://journal.uir.ac.id/index.php/dinamikapertanian/article/view/802
Schleheck, D. (2004). Biodegradation of synthetic surfactants: linear alkylbenzenesulfonates (LAS) and related compounds (Doctoral dissertation, Verlag nicht ermittelbar). https://kops.uni-konstanz.de/server/api/core/bitstreams/ca6d822d-5067-4e10-a1a6-7af78f5bf938/content
Sidik, N. R. (2009). Kajian Pengaruh Konsentrasi Metil Ester Sulfonat (Mes) dan Konsentrasi Alkali (KOH) Terhadap Kinerja Deterjen Cair Industri (Doctoral dissertation, IPB (Bogor Agricultural University)). https://repository.ipb.ac.id/handle/123456789/19637
Sumarwanto, P., & Hartati, Y. (2018). Penanganan Air Limbah Cucian Alat Gelas Laboratorium dengan Metode Spektrofotometri Menggunakan Pereaksi Biru Metilen. Indonesian Journal of Laboratory, 1(1), 10-15. https://doi.org/10.22146/ijl.v1i1.40964
Suriani, S., Suharjono, S., & Soemarno, S. (2015). Potensi Bakteri Genus Pseudomonas Pendegradasi LAS di Ekosistem Sungai Tercemar Deterjen Sekitar Kampus Universitas Brawijaya. Jurnal Pembangunan dan Alam Lestari, 6(1). https://jpal.ub.ac.id/index.php/jpal/article/view/182
Victoria, S., Sitorus, S., & Panggabean, A. S. (2022). Verifikasi Parameter Pengujian Surfaktan Anionik Dalam Air Limbah Dengan Metode Mbas Menggunakan Spektrofotometer UV-VIS. Jurnal Atomik, 7(2), 15-19. https://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/1113
Wikaningrum, T., & Pratamadina, E. (2022). Potensi Penggunaan Eco Enzyme sebagai Biokatalis dalam Penguraian Minyak dan Lemak pada Air Limbah Domestik. Jurnal Serambi Engineering. 7(4):3924-32. 10.32672/jse.v7i4.4849
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