Isolation and Characterization of Endophytic Fungi in Ramin (Gonystylus bancanus Miq. Kurz) from Central Kalimantan
DOI:
10.29303/jbt.v26i1.11419Published:
2026-03-27Downloads
Abstract
Ramin (Gonystylus bancanus Miq. Kurz) is one of the typical woody plants that live in peat swamp forest ecosystems, especially in Central Kalimantan. Ramin plants have endophytic fungi that live in plant cells that can contaminate tissue culture propagation. In addition, endophytic fungi in ramin plants have not been widely studied, both in terms of characterization and potential. This study aimed to identify and analyze endophytic fungi residing in ramin plants. The methods used were media preparation and tool preparation, isolation of endophytic fungi, macro- and microscopic characterization of endophytic fungi, and data analysis. This study successfully isolated five endophytic fungi from G. bancanus plants, including 4 isolates (BR1, BR2, BR3, BR4) derived from stems and 1 isolate (BDR) derived from stems and leaves. The characters of these endophytic fungi are white and black in color, smooth and rough texture, and hyphae with and without hyphae (asepta). Endophytic fungi in ramin plants pose a challenge for propagating ramin tissue culture because they become contaminants. However, endophytic fungi certainly have their own benefits in the bodies of ramin plants and other potential benefits for humans. This research can serve as an initial study to determine the potential of endophytic fungi in ramin plants and to provide new information on handling contaminants in ramin plant tissue culture.
Keywords:
Characterization Endophyt Fungi Isolation RaminReferences
Abna, I. M., Nurfitria, S., & Widyaswari Mahayasih, P. G. M. (2024). Analisis Antimikroba Jamur Endofit Daun Dan Batang Tumbuhan Kelor (Moringa Oleifera Lam.). Journal Of Pharmacopolium, 7(2). Https://Doi.Org/10.36465/Jop.V7i2.1372
Aji, O. R., Sari, A. K., & Putri, D. A. (2022). Isolasi Dan Uji Aktivitas Antagonisme Jamur Endofit Tanaman Pisang (Musa Paradisiaca L.) Terhadap Fusarium Oxysporum. Bioscientist : Jurnal Ilmiah Biologi, 10(1), 10. Https://Doi.Org/10.33394/Bioscientist.V10i1.4718
Ali, M., & Samosir, I. Y. (2022). Uji Antagonisme Jamur Endofit Tanaman Aren (Arenga Pinnata Merr.) Terhadap Ganoderma Boninense Pat. Penyebab Penyakit Busuk Pangkal Batang Kelapa Sawit. Agrikultura, 32(3), 304. Https://Doi.Org/10.24198/Agrikultura.V32i3.36611
Andriani, D., & Heriansyah, P. (2021). Identifikasi Jamur Kontaminan Pada Berbagai Eksplan Kultur Jaringan Anggrek Alam (Bromheadia Finlaysoniana (Lind.) Miq. Agro Bali : Agricultural Journal, 4(2), 192–199. Https://Doi.Org/10.37637/Ab.V4i2.723
Antonius. (2024). Study Plant Ramin (Gonystylus Bancanus) Of Peat Swamp Forest In Baning Nature Tourist Park Area In Sintang Regency. 20(1), 80–89. Http://Jurnal.Unka.Ac.Id/Index.Php/Piper
Bahri, S., Amelia, P., Hardini, A., Ramadhan, F., Azmi Muhammad, A., & Kahfi, M. (2021). Aktivitas Antibakteri Kapang Endofit Dari Kulit Batang Tanaman Kayu Jawa (Lannea Coromandelica (Hout.) Merr.) Terhadap Bakteri Streptococcus Mutans Dan Shigella Dysenteriae. Jurnal Biotek Medisiana Indonesia, 10(1). DOI:10.22435/JBMI.V10I1.5028
Bahri, S., Christy, T., Nanda Setiawan, Y., Septianingsih, V., Ikhsan, M., Triutami, F., Noviyanti, A., Permata Utari Andini, P., & Ramadhan, F. (2023). Uji Antimikroba Ekstrak Kapang Endofit Rlc 5 Akar Tanaman Kayu Jawa (Lannea Coromandelica (Houtt.) Merr.). Pharmaceutical Journal Of Indonesia, 9(1), 65–70. Https://Doi.Org/10.21776/Ub.Pji.2023.009.01.10
Barnett, H. L., & Hunter, B. B. (1998). Illustrated Genera Of Imperfect Fungi. American Phytopathological Society. Minneapolis, Burgess Publishing Company.
Decenly, D., Simbolon, S. G. G., Sunariyati, S., Anggoro, R. O., & Guevara, K. A. R. (2025). Effect Of Iba Concentration On The Growth Of Ramin Cuttings (Gonystylus Bancanus (Mig.) Kurz). Jurnal Biologi Tropis, 25(1), 529–535. Https://Doi.Org/10.29303/Jbt.V25i1.8485
Elfina, Y., Ali, M., Wulandari, S. F., & Ibrahim, R. (2022). Identifikasi Morfologi Lima Isolat Jamur Endofit Tanaman Bawang Merah Dan Kemampuannya Menghambat Alternaria Porri Ellis Cif. Terakreditasi Ristekbrin Peringkat Sinta, 18(1), 2020. Https://Doi.Org/10.30598/Jbdp.2022.18.1.74
Ellis, D., Davis, S., Alexiou, H., Handke, R., & Bartley, R. (2007). Description Of Medical Fungi, Second Edition. Mile End, South Australia: Nexus Print Solutions.
Erliza, M., & Lubis, S. S. (2023). Karakterisasi dan Uji Potensi Jamur Endofit Pada Daun Bawang Merah (Allium ascalonicum L.) Sebagai Pengendali Patogen Fusarium sp. dan Alternaria sp. KENANGA : Journal of Biological Sciences and Applied Biology, 3, 35–53. https://doi.org/10.22373/kenanga.v3i1.3120
Fadiji, A. E., & Babalola, O. O. (2020). Exploring the potentialities of beneficial endophytes for improved plant growth. Saudi Journal of Biological Sciences, 27(12), 3622–3633. https://doi.org/10.1016/j.sjbs.2020.08.002
Fitri, T., Pujawati, E. D., & Payung, D. (2021). Pengaruh Pemberian Rootone F Terhadap Pertumbuhan Stek Ramin (Gonystylus Bancanus). Jurnal Sylva Scienteae, 4(1), 174. Https://Doi.Org/10.20527/Jss.V4i1.3105
Gandjar, I., Samson, R. A., Van Den Tweel-Vermeulen, K., Oetari, A., & Santoso, I. (1999). Pengenalan Kapang Tropik Umum. Jakarta : Yayasan Obor Indonesia.
Grabka, R., D’entremont, T. W., Adams, S. J., Walker, A. K., Tanney, J. B., Abbasi, P. A., & Ali, S. (2022). Fungal Endophytes And Their Role In Agricultural Plant Protection Against Pests And Pathogens. Plants, 11(3), 384. Https://Doi.Org/10.3390/Plants11030384
Grippin, A., Nor Aini, A. S., Nor Akhirrudin, M., Hazandy, A. H., Sures Kumar, M., & Ismail, P. (2018). The Prospect Of Micropropagating Gonystylus Bancanus (Miq.) Kurz, A Tropical Peat Swamp Forest Timber Species Through Tissue Culture Technique - Review. Journal Of Forest Science, 64(1), 1–8. Https://Doi.Org/10.17221/130/2017-Jfs
Harmileni, H., Saragih, G., Hidayani, T. R., Mirnandaulia, M., Ginting, C. N., & Fachrial, E. (2023). Mikroba Endofit Dalam Dunia Kesehatan Manfaat Dan Aplikasi. Medan : Unpri Press.
Hasiani, V. V., Ahmad, I., & Rijai, L. (2015). Isolasi Jamur Endofit Dan Produksi Metabolit Sekunder Antioksidan Dari Daun Pacar (≪I≫Lawsonia Inermis≪/I≫ L.). Jurnal Sains Dan Kesehatan, 1(4), 146–153. Https://Doi.Org/10.30872/Jsk.V1i4.41
Heriyanto, N. M., Garsetiasih, D. R., Litbang, P., Dan, H., & Alam, K. (2006). Ekologi Dan Potensi Ramin (Gonystylus Bancanus Kurz.) di Kelompok Hutan Sungai Tuan-Sungai Suruk, Kalimantan Barat. Buletin Plasma Nutfah, 12 : 24-29.
Imaningsih, W., Nur, H. S., & Susilawati, I. O. (2019). Isolasi Dan Karakterisasi Kapang Endofit Asal Tanaman Ulin (Eusideroxylon Zwegeri T Et B). Laporan Penelitian. Universitas Lambung Mangkurat.
Karni, F., Naemah, D., Program, S. B., & Kehutanan, S. (2021). Identifikasi Kerusakan Tegakan Ramin (Gonystylus Bancanus) Di Punggualas Taman Nasioanal Sebangau Kalimantan Tengah Identification Of Ramin (Gonystylus Bancanus) Damage In Punggualas Sebangau National Park Central Kalimantan. In Jurnal Sylva Scienteae 04 (3) :561-569. DOI: https://doi.org/10.20527/jss.v4i3.3758
Khairiah, N., & Rinne, N. (2018). Isolasi Dan Uji Aktivitas Antimikroba Kapang Endofit Dari Kayu Ulin (Eusideroxylon Zwageri Teijsm & Binn.). Jurnal Riset Industri Hasil Hutan, 9(2), 65-74. DOI: 10.24111/jrihh.v9i2.3373
Milinia, J., Sari, P., Adrian, R., & Lubis, B. (2022). Review: Jamur endofit sebagai biokontrol dan pemacu pertumbuhan tanaman pangan dan hortikultura di lahan suboptimal. Prosiding Seminar Nasional Lahan Suboptimal Ke-10.
Nair, D. N., & Padmavathy, S. (2014). Impact of Endophytic Microorganisms on Plants, Environment, and Humans. The Scientific World Journal, 1–11. https://doi.org/10.1155/2014/250693
Nurfitri, R., Hardini, J., & Ketut Muksin. (2024). Identifikasi jamur endofit yang berasosiasi dengan daun jeruk limau Citrus × amblycarpa (Hassk.) Ochse di Kebun Kalpataru, Denpasar, Bali. SIMBIOSIS XII, 100–107. https://doi.org/https://doi.org/10.24843/JSIMBIOSIS.2024.v12.i01.p11
Nurhasybi, Komar, T. E., & Sumbayak, E. S. S. (2010). Manual Monitoring Musim Berbunga-Berbuah Dan Produksi Benih Ramin (Gonystylus Bancanus). Bogor: Itto Cites Project. Pusat Penelitian dan Pengembangan Hutan dan Konservasi Alam, Kementerian Kehutanan.
Pancaningtyas, S., & Nafi’ah, R. (2020). Identifikasi Jamur Kontaminan Pada Tahapan Inisiasi Eksplan Kultur In Vitro Kakao (Theobroma Cacao L). Warta - Pusat Penelitian Kopi Dan Kakao Indonesia, 32(2).
Putri, I. P., Herawan, T., Prastyono, P., & Haryjanto, L. (2017). Pengaruh Teknik Sterilisasi Explan Terhadap Tingkat Perolehan Kultur Jaringan Aksenik Ramin (Gonystylus Bancanus). Jurnal Pemuliaan Tanaman Hutan, 11(2), 131–138. Https://Doi.Org/10.20886/Jpth.2017.11.2.131-138
Rahayu, R. S., Ramadhani, I., Riastiwi, I., Dyah Prawestri, A., & Yuliani, Y. (N.D.). Konfirmasi Mikroba Endofit Penyebab Kontaminasi Pada Kultur Jaringan Kangkung (Ipomoea Aquatica Forssk.). Jurnal Bioteknologi & Biosains Indonesia (JBBI) 7(2):234-249. DOI: 10.29122/jbbi.v7i2.4381
Rampa, E., Manurun, S., & Sinaga, H. (N.D.). Isolasi Fungi Endofit Dari Buah Dan Daun Belimbing Wuluh (Averrhoa Bilimbi L.) Jurnal Penelitian Kesehatan Suara forkes, 14 (1): 92-96. DOI: http://dx.doi.org/10.33846/sf14119
Riga, R., Silvani, M. A., Oktria, W., Nasra, E., Kurniawati, D., & Agustini, D. M. (2024). Jamur Endofitik Bjs-3 Asosiasi Sambiloto (Andrographis Paniculata): Skrining Fitokimia Dan Aktivitas Antioksidan. Jurnal Sains Dan Kesehatan, 5(5), 695–700. Https://Doi.Org/10.30872/Jsk.V5i5.573
Sidiyasa, K., Mansur, M., Triono, T., & Rachman, I. (N.D.). Panduan Identifikasi Jenis-Jenis Ramin (Gonystylus Spp.) Di Indonesia. Bogor: Itto Cites Project. Pusat Penelitian dan Pengembangan Hutan dan Konservasi Alam, Kementerian Kehutanan.
Soesanto, L., & Mugiastuti. (2023). Mikroba Endofit: Eksplorasi. Potensi Dan Pemanfaatan Mikroba Endofit Bagi Kesehatan Tanaman Dan Manusia Serta Keuntungan Ekonomi. Lily Publisher, Penerbit Andi: Yogyakarta.
Surdiah, S., Nofiani, R., & Ardiningsih, P. (2020). Isolasi Dan Identifikasi Serta Uji Aktivitas Antibakteri Fungi Endofit Gaharu Dari Aquilaria Sp. Jurnal Kimia Khatulistiwa, 8(4), 13–18. https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/42726
Waqar, S., Bhat, A. A., & Khan, A. A. (2024). Endophytic Fungi: Unravelling Plant-Endophyte Interaction And The Multifaceted Role Of Fungal Endophytes In Stress Amelioration. Plant Physiology And Biochemistry, 206, 108174. Https://Doi.Org/10.1016/J.Plaphy.2023.108174
Watanabe, T. (2002). Pictorial Atlas Of Soil And Seed Fungi. Boca Raton: Crc Press. https://doi.org/10.1201/EBK1439804193
Wathan, N., Viogenta, P., Azizah, J., Ramadhan, F., & Sari, S. R. (2023). Studi Fitokimia Jamur Endofit Tumbuhan Seluang Belum (Luvunga Sarmentosa (Blume) Kurz) Asal Kabupaten Tabalong Kalsel. Jurnal Pharmascience, 10(1), 51–57. DOI:
10.20527/jps.v10i1.14317
Wen, J., Okyere, S. K., Wang, S., Wang, J., Xie, L., Ran, Y., & Hu, Y. (2022). Endophytic Fungi: An Effective Alternative Source of Plant‐Derived Bioactive Compounds for Pharmacological Studies. In Journal of Fungi (Vol. 8, Number 2). MDPI. https://doi.org/10.3390/jof8020205
Wijesekara, T., & Xu, B. (2023). Health-Promoting Effects of Bioactive Compounds from Plant Endophytic Fungi. In Journal of Fungi (Vol. 9, Number 10). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/jof9100997
Yelnititis, Y., & Edy Komar, T. (2011). Mikropropagasi Ramin (Gonytilus Bancanus(Miq) Kurz) Dari Eksplan Batang Satu Buku Secara In Vitro. Jurnal Pemuliaan Tanaman Hutan, 5(3), 149–157. Https://Doi.Org/10.20886/Jpth.2011.5.3.149-157
Yuliani, W., & Ismail, R. (2023). Uji Aktivitas Antijamur Fungi Endofit Tanaman Sarang Semut (Myrmecodia Pendans) Terhadap Jamur Candida Albicans. Pharmacy Genius, 2(1), 31–42. Https://Doi.Org/10.56359/Pharmgen.V2i1.172
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