Physical and Biochemical Characteristics of Eco-Enzyme Solutions as Biology Learning Resources for Senior High Schools
DOI:
10.29303/jbt.v26i2.11545Published:
2026-05-26Downloads
Abstract
The Merdeka Curriculum emphasizes student-centered and experiment-based learning that utilizes the surrounding environment as a contextual learning resource. This study analyzes the physical characteristics (pH, color, and odor) and biochemical properties (primary and secondary metabolites) of ecoenzyme solutions made from molasses and palm sugar. This study utilized an experimental approach and generated quantitative descriptive data to identify differences in physical and biochemical characteristics between the two solutions. The results show variations in pH, odor intensity, color, and metabolite content, indicating that the type of raw material influences the properties of the ecoenzyme. These findings can be used as contextual learning resources for senior high school and vocational health students in biology and chemistry learning under the Merdeka Curriculum. In addition, the analysis of primary metabolites (reducing sugars, proteins, organic acids, and lipids) and secondary metabolites (alkaloids, saponins, tannins, flavonoids, and coumarins) provides meaningful scientific evidence that links fermentation processes with observable chemical changes. This integration of laboratory investigation and real-life environmental materials supports the development of students’ scientific literacy, critical thinking, and experimental skills through contextual and inquiry-based learning activities.
Keywords:
Biochemical characterictic Contextual learning Eecoenzyme Merdeka curriculum Physical characteristicsReferences
Afa, M., Irwansyah, I., & Junaedi, J. (2024). Uji Kualitas Pupuk Organik Cair (POC) berbahan Dasar Jeroan Ayam Menggunakan Mikroorganisme Lokal (MOL) dari Limbah Buah sebagai Dekomposer. Tarjih Tropical Livestock Journal, 4(2), 45-52. DOI: https://doi.org/10.47030/trolija.v4i2.832
Amri, K., Latuconsina, H., Triyanti, R., Setyanto, A., Prayogo, C., Wiadnya, D. G. R., ... & Ramlan, A. (2023). Pengelolaan Sumber Daya Perikanan Laut Berkelanjutan. Penerbit BRIN. DOI: https://doi.org/10.55981/brin.908
Arun, C., & Sivashanmugam, P. J. P. S. (2015). Investigation of biocatalytic potential of garbage enzyme and its influence on stabilization of industrial waste activated sludge. Process Safety and Environmental Protection, 94, 471-478. DOI: https://doi.org/10.1016/j.psep.2014.10.008
Benedict, S. R. (1909). A reagent for the detection of reducing sugars. Journal of Biological Chemistry, 5(5), 485–487.DOI: https://doi.org/10.1016/S0021-9258(18)93090-2
Bourgaud, F., Hehn, A., Larbat, R., Doerper, S., Gontier, E., Kellner, S., & Matern, U. (2006). Biosynthesis of coumarins in plants: a major pathway still to be unravelled for cytochrome P450 enzymes. Phytochemistry Reviews, 5(2), 293-308.
Castañeda-Ovando, A., de Lourdes Pacheco- Hernández, M., Páez-Hernández, M. E., Rodríguez, J. A., & Galán-Vidal, C. A. (2009). Chemical studies of anthocyanins: A review. Food chemistry, 113(4), 859-871. DOI: https://doi.org/10.1016/j.foodchem.2008.09.001
Eskundari, R. D., Wardoyo, S. H., Cahyanti, F. A., Fitriani, R. D. A., & Saputra, D. A. (2023). Effect of Ecoenzim Solution on Balsam Plant (Impatiens balsamina L.) Growth. Jurnal Biologi Tropis, 23(3), 143-147. DOI: DOI: 10.29303/jbt.v23i3.5023
Eskundari, R. D., Wiharti, T., Hanik, N. R., Fatimah, F., Salamah, U., & Murwani,A. (2022). Phytochemical test of several eco-handsanitizer candidates. Jurnal Biologi Tropis, 22(1), 297-303. DOI: 10.29303/jbt.v22i1.3258
Fermentation, identification, and antioxidant activity of saponins produced by a wild ginseng endophytic fungus Umbelopsis dimorpha strain NSJG. Fermentation, 10(1), 9 DOI: https://doi.org/10.3390/molecules28166020
Gornall, A. G., Bardawill, C. J., & David, M. M. (1949). Determination of serum proteins by means of the Biuret reaction. Journal of Biological Chemistry, 177(2), 751–766.
DOI: https://doi.org/10.1016/S0021-9258(18)57021-4
Gupta. Phytochemical screening and GC-MS analysis of flower extract of Dillenia indica. Biosci Biotechnol res comm, 13(2), 833-841. DOI: https://doi.org/10.1016/j.matpr.2019.09.170
Hack, M. H. (1953). Analysis of lipids by spot tests on filter-paper disk chromatograms. Journal of Lipid Research, ??(??), ??–??.
DOI: https://doi.org/10.1016/S0021-9258(18)57021-4
Harborne, A. J. (1998). Phytochemical methods a guide to modern techniques of plant analysis. springer science & business media.
Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (3rd ed.). Springer.
DOI: https://doi.org/10.1007/978-94-Sadasivam, S., & Manickam, A. (2008). Biochemical Methods (3rd ed.). New Age International Publishers.
DOI: https://doi.org/10.1201/9781439823184 009-5570-7
Hardiningsih, D. T., Asty, Z. F., Delfira, A., & Ayudia, E. I. (2025). Qualitative confirmation of flavonoids in Moringa oleifera leaf extract and evaluation of antioxidant potential. Proceedings Academic Universitas Jambi, 1(2), 503-508. DOI: https://doi.org/10.22437/proca.v1i2.50311
Hur, S. J., Lee, S. Y., Kim, Y. C., Choi, I., & Iqbal, M., & Jariah, A. A. (2025). Phytochemical screening of secondary metabolite compounds in tammate leaf extract (Lannea coromandelica (HOUTT.) MERR.) from Pangkep Regency using various extraction methods. Journal of Current Health Sciences, 5(1), 59-66.
Joshi, S., & Bhosale, P. (2024). Qualitative Tests for Lipid Detection. Dalam Practical Biochemistry (hlm. 217–233). Bentham Science Publishers.
https://doi.org/10.2174/9789815165852124010018
Julinar, J., Uswati, D., Riyanti, F., & Musifa, E. (2025). Ecoenzyme Characterization of Fruit Peel Waste Mixture and Test of Antibacterial Activity against Bacteria Causing Dental Caries. IJFAC (Indonesian Journal of Fundamental and Applied Chemistry), 10(3), 186-197. DOI: DOI: 10.24845/ijfac.v10.i3.186
Kamaliya, N., & Lusiani, C. E. (2023). Effect Of Baker’s Yeast Concentrations On Eco Enzyme Products By The Fermentation Process. Distilat: Jurnal Teknologi Separasi, 9(4), 412-24. DOI: DOI: https://doi.org/10.33795/distilat.v9i4.4173
Kesmayanti, N., Purwanto, R. J., Romza, E., Kalsum, U., Irmawati, W., & Putri, K. (2025). Effectiveness of Eco-Enzyme for Increasing Growth and Production of Shallot from Vegetative Seeds. Journal of Agriculture, 4(02), 106-114. DOI:https://doi.org/10.47709/joa.v4i02.6442
Khafid, A., Wiraputra, M. D., Putra, A. C., Khoirunnisa, N., Putri, A. A. K., Suedy,
Kim, G. B. (2014). Effect of fermentation on the antioxidant activity in plant-based foods. Food chemistry, 160, 346-356.
Kumar, M. K., Kaur, G., & Kaur, H. (2011). Internationale Pharmaceutica Sciencia.
Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The scientific world journal, 2013(1), 162750. DOI:
Lawless, H. T., & Heymann, H. (2010). Sensory evaluation of food: principles and practices. Springer Science & Business Media. DOI: https://doi.org/10.1007/978-1-4419-6488-5
Li, J., Ma, D., Tian, J., Sun, T., Meng, Q., Li, J., & Shan, A. (2023). The responses of organic acid production and microbial community to different carbon source additions during the anaerobic fermentation of Chinese cabbage waste. Bioresource technology, 371, 128624.
Godlewska, K., Pacyga, P., Szumny, A., Szymczycha-Madeja, A., Wełna, M., & Michalak, I. (2022). Methods for rapid screening of biologically active compounds present in plant-based extracts. Molecules, 27(20), 7094. https://doi.org/10.3390/molecules27207094
Mores, R. (2026). Continuous Training and Development Program in Hospitality Industries. Journal of Current Research in Business and Economics, 5(1), 3629-3671.
Mulyani, S., Sunarko, K. M. F., & Setiani, B. E. (2021). Pengaruh Lama Fermentasi terhadap Total Asam, Total Bakteri Asam Laktat dan Warna Kefir Susu Kacang Merah. Jurnal Ilmiah Sains, 21(2), 113–118. DOI: https://doi.org/10.35799/jis.21.2.2021.31416
Mycochemical screening and analysis, antioxidant activity, and biochemical composition of fermentation strain Snef1216 (Penicillium chrysogenum). Journal of Analytical Methods in Chemistry, 2020(1), 3073906.
Ningsih, D. S., Henri, H., Roanisca, O., & Mahardika, R. G. (2020). Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-Sapu (Baeckea frutescens L.). Biotropika: Journal of Tropical Biology, 8(3), 178-185.
Resti, N. (2025). Pengembangan E- Module Berbasis Gamifikasi Untuk Meningkatkan Literasi Lingkungan Dan Motivasi Belajar Peserta Didik Pada Materi Pemanasan Global.
Rukmini, P., & Herawati, D. A. (2023). Eco- enzyme from Organic Waste (Fruit and Rhizome Waste) Fermentation: Eco- Enzyme Dari Fermentasi Sampah Organik (Sampah Buah Dan Rimpang). Jurnal Kimia dan Rekayasa, 4(1), 23-2 DOI: DOI: https://doi.org/10.31001/jkireka.v4i1.62
Rusu, A. V., Trif, M., & Rocha, J. M. (2023). Microbial secondary metabolites via fermentation approaches for dietary supplementation formulations. Molecules, 28(16), 6020. https://doi.org/10.3390/molecules28166020
S. W. A., & Nurchayati, Y. (2023). Uji kualitatif metabolit sekunder pada beberapa tanaman yang berkhasiat sebagai obat tradisional. Buletin Anatomi Dan Fisiologi, 8(1), 61-70. DOI: https://doi.org/10.14710/baf.8.1.2023.61-70
Sabila, L. (2025). Perkembangan Kurikulum Merdeka Pendidikan Ips Di Indonesia Permasalahan Dan Urgensi. Pendas: Jurnal Ilmiah Pendidikan Dasar, 10(02), 211-224.
Sadasivam, S., & Manickam, A. (2008). Biochemical Methods (3rd ed.). New Age International Publishers.
DOI: https://doi.org/10.1201/9781439823184
Santosa, S., Hassan, M. S., & Kasim, A. H. (2023). Ecoenzym quality and potential of residues as bioactivator for organic waste composting. Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management), 13(3).
Sawant, S. S., Park, H. Y., Sim, E. Y., Kim, H. S., & Choi, H. S. (2025). Microbial Fermentation in Food: Impact on Functional Properties and Nutritional Enhancement—A Review of Recent Developments. Fermentation (Basel), 11(1).
Skoog, D. A., West, D. M., Holler, F. J., & Crouch, S. R. (1996). Fundamentals of analytical chemistry (Vol. 33, pp. 53-55). Fort Worth: Saunders College Pub. DOI: https://doi.org/10.1007/978-1-133-71413-7
Sreevidya, N., & Mehrotra, S. (2003). Spectrophotometric method for estimation of alkaloids precipitable with Dragendorff’s reagent in plant materials. Journal of AOAC International, 86(6), 1124–1127.
DOI: https://doi.org/10.1093/jaoac/86.6.1124
Stone, H., Bleibaum, R. N., & Thomas, H. A. (2020). Sensory evaluation practices. Academic press. DOI: https://doi.org/10.1007/978-1-4419-6488-5
Stone, H., Bleibaum, R. N., & Thomas, H. A. (2020). Sensory evaluation practices. Academic press. DOI: https://doi.org/10.1016/C2010-0-66248-9
Triyatdipa, H., Agustien, A., & Marlina, M. (2025). A Analysis of Ecoenzyme Characteristics from Combination of Fruit Peel Waste. Biospecies, 18(2), 11-
Venugopala, K. N., Rashmi, V., & Odhav, B. (2013). Review on natural coumarin lead compounds for their pharmacological activity. BioMed research international, 2013(1), 963248.
Vinsur, E. Y. Y., & Sipollo, B. V. (2025). Eco- enzyme Apple Bar Soap for Skin Hydration in Older Adults. Babali Nursing Research, 6(2), 308-322.
WIDIA Y, Y. N. (2025). Pengaruh Model Pembelajaran Science, Environment, Technology, And Society Berbantuan Asesmen Kinerja Terhadap Literasi Sains Dan Kecerdasan Emosional Kelas X Pada Mata Pelajaranbiologi (Doctoral dissertation, UIN RADEN INTAN LAMPUNG).
Widjanarko, S. B., Latifa, P. A., & Yumna, K. (2023). Profil Sifat Fisiko-Kimia dan Mikrobiologi Cairan Eco-Enzymedari Beberapa Jenis Buah dan Daun Cemara Udang (Casuarina equisetifolia). Jurnal Teknologi Pertanian, 23(2), 117-126.
Yolanda, P. (2023). Pengaruh Model Ricosre Terhadap Keterampilan Berpikir Kritis Dan Sikap Ilmiah Peserta Didik Pada Mata Pelajaran Biologi KELAS XI SMA (Doctoral dissertation, UIN RADEN INTAN LAMPUNG).
License
Copyright (c) 2026 Fadhillah Marta, Ratna Dewi Eskundari

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.























