Zinc (Zn) and Iron (Fe) Residues in Cattle Liver: A Comparative Study in Mining and Non-Mining Zones
DOI:
10.29303/jbt.v26i2.11728Published:
2026-05-01Downloads
Abstract
Laterite nickel mining activities in Southeast Sulawesi significantly contribute to environmental contamination, potentially leading to the bioaccumulation of heavy metals in free-grazing livestock. The liver, functioning as a primary metabolic organ, serves as a sensitive bioindicator for heavy metal exposure. This study aimed to analyze and compare the concentrations of essential heavy metals, Zinc (Zn) and Iron (Fe), in the liver of cattle reared in mining circum-areas (Sopura and Oko-oko) versus non-mining control areas (Toari and Watubangga). Liver samples were collected from 12 cattle (n = 3 per location). The samples were prepared using wet digestion and analyzed using Atomic Absorption Spectrophotometry (AAS). Data were analyzed using Independent Sample T-test and One-Way ANOVA followed by Tukey HSD post-hoc test. The results showed a significant difference (p < 0.05) in metal concentrations between mining and non-mining areas. The mining area of Oko-oko exhibited an extreme accumulation of Iron (Fe), reaching 1,803.40 ± 48.64 mg/kg, which is significantly higher than the control area of Watubangga (47.69 ± 1.94 mg/kg). Zinc (Zn) levels were also significantly higher in mining areas (21.88–24.22 mg/kg) compared to non-mining areas (2.97–8.30 mg/kg). There is a strong correlation between mining activities and the bioaccumulation of Zn and Fe in bovine liver. The extreme iron levels detected in the mining zone suggest a potential health risk for consumption, necessitating the implementation of depuration strategies and stricter food safety monitoring in the affected regions.
Keywords:
Bioaccumulation Bovine Liver Heavy Metals Nickel Mining Food Safety Zinc IronReferences
Afzal, A., & Mahreen, N. (2024). Emerging insights into the impacts of heavy metals exposure on health, reproductive and productive performance of livestock. Frontiers in Pharmacology, 15, Article 1375137. https://doi.org/10.3389/fphar.2024.1375137
Akele, M. L., Desalegn, S. K., Asfaw, T. B., Assefa, A. G., Alemu, A. K., & de Oliveria, R. R. (2022). Heavy metal contents in bovine tissues (kidney, liver and muscle) from Central Gondar Zone, Ethiopia. Heliyon, 8(12), Article e12416. https://doi.org/10.1016/j.heliyon.2022.e12416
Akil, S., Damayanti, E., Samsudewa, D., Hapila, A., & Iskandar, H. (2025). Effects of zinc supplementation on reproductive performance in cattle: A review. Jurnal Bioteknologi dan Biosains Indonesia, 12(1), 163–170. https://doi.org/10.55981/jbbi.2025.11335
Association of Official Analytical Chemists. (2016). Official methods of analysis of AOAC International (20th ed.). AOAC International.
Aydemir, T. B., & Cousins, R. J. (2018). The multiple faces of the metal transporter ZIP14 (SLC39A14). Journal of Nutrition, 148(2), 174–184. https://doi.org/10.1093/jn/nxx041
Badan Standardisasi Nasional. (2009). SNI 7387:2009: Batas maksimum cemaran logam berat dalam pangan. Badan Standardisasi Nasional.
Bataa, B., Motohira, K., Dugar, D., Sainnokhoi, T.-A., Gendenpil, L., Sainnokhoi, T., Pelden, B., Yohannes, Y. B., Ganzorig, S., Nakayama, S. M. M., Ishizuka, M., & Ikenaka, Y. (2022). Accumulation of metals in the environment and grazing livestock near a Mongolian mining area. Toxics, 10(12), Article 773. https://doi.org/10.3390/toxics10120773
Briffa, J., Sinagra, E., & Blundell, R. (2020). Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon, 6(9), Article e04691. https://doi.org/10.1016/j.heliyon.2020.e04691
Chen, F., Muhammad, F. G., Khan, Z. I., Ahmad, K., Naz, S., Hussain, M. I., Siddiqui, M. H., Al-Hashimi, A., & Bungau, S. (2022). Bioaccumulation and transfer of zinc in soil plant and animal system: A health risk assessment for the grazing animals. Environmental Science and Pollution Research, 29(2), 2718–2727. https://doi.org/10.1007/s11356-021-15808-z
Chen, J., Li, X., Ge, C., Min, J., & Wang, F. (2022). The multifaceted role of ferroptosis in liver disease. Cell Death & Differentiation, 29(3), 467–480. https://doi.org/10.1038/s41418-022-00941-0
Counotte, G., Holzhauer, M., van Dijken, S. C., Muskens, J., & Van der Merwe, D. (2019). Levels of trace elements and potential toxic elements in bovine livers: A trend analysis from 2007 to 2018. PLOS ONE, 14(4), Article e0214584. https://doi.org/10.1371/journal.pone.0214584
Duffy, R., Yin, M., & Redding, L. E. (2023). A review of the impact of dietary zinc on livestock health. Journal of Trace Elements and Minerals, 5, Article 100085. https://doi.org/10.1016/j.jtemin.2023.100085
Fathi-Beyranvand, A., Rashidimehr, A., Askari, E., Esfarjani, F., & Mohammadi-Nasrabadi, F. (2025). Potentially toxic metals in small ruminant tissues: Multivariate analysis and health risk assessment via Monte Carlo simulation. Scientific Reports, 16, Article 3704. https://doi.org/10.1038/s41598-025-33838-2
Gunawan, Priyanto, R., & Salundik. (2015). Analisis lingkungan sekitar tambang nikel terhadap kualitas ternak sapi pedaging di Kabupaten Halmahera Timur. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 3(1), 59–64. https://doi.org/10.29244/jipthp.3.1.59-64
Hati, S. N. A. P., Hapsari, M. A., Ika, S. R., & Widagdo, A. K. (2024). Nickel ore export prohibition and mapping the business performance of nickel mining companies in Indonesia. IOP Conference Series: Earth and Environmental Science, 1412, Article 012026. https://doi.org/10.1088/1755-1315/1412/1/012026
Hill, G. M., & Shannon, M. C. (2019). Copper and zinc nutritional issues for agricultural animal production. Biological Trace Element Research, 188(1), 148–159. https://doi.org/10.1007/s12011-018-1578-5
Hu, X., Fan, Y., Lv, Y., Yang, X., Yue, Z., Wang, J., Li, H., Chen, X., Zhang, J., & Zhao, X. (2025). Zinc and animal health: An in-depth exploration of its role in physiological functions and regulatory molecular mechanisms. Journal of Animal Science and Biotechnology, 16, Article 36. https://doi.org/10.1186/s40104-025-01301-x
Ilham, Hartono, D. M., Suganda, E., & Nurdin, M. (2017). Metal distribution at river water of mining and nickel industrial area in Pomalaa Southeast Sulawesi Province, Indonesia. Oriental Journal of Chemistry, 33(5), 2599–2607. https://doi.org/10.13005/ojc/330557
Joint FAO/WHO Expert Committee on Food Additives. (2017). Safety evaluation of certain food additives and contaminants (WHO Food Additives Series No. 76). World Health Organization. https://apps.who.int/food-additives-contaminants-jecfa-database/chemical.aspx?chemID=4197
Kusuma, R. A. I., Kamaruddin, H., Rosana, M. F., & Yuningsih, E. T. (2019). Geokimia endapan nikel laterit di Tambang Utara, Kecamatan Pomalaa, Kabupaten Kolaka, Provinsi Sulawesi Tenggara. Jurnal Geologi dan Sumberdaya Mineral, 20(2), 85–92. https://doi.org/10.33332/jgsm.geologi.v20i2.435
López-Alonso, M., Benedito, J. L., Miranda, M., Castillo, C., Hernandez, J., & Shore, R. F. (2000). Arsenic, cadmium, lead, copper and zinc in cattle from Galicia, NW Spain. Science of the Total Environment, 246(2–3), 237–248. https://doi.org/10.1016/S0048-9697(99)00461-1
López-Alonso, M., Benedito, J. L., Miranda, M., Castillo, C., Hernandez, J., & Shore, R. F. (2002). Cattle as biomonitors of soil arsenic, copper, and zinc concentrations in Galicia (NW Spain). Environmental Research, 88(2), 96–103. https://doi.org/10.1006/enrs.2001.4312
López-Alonso, M., Prieto-Montaña, F., Miranda, M., Castillo, C., Hernandez, J., & Benedito, J. L. (2004). Interactions between toxic (As, Cd, Hg and Pb) and nutritional essential (Ca, Co, Cr, Cu, Fe, Mn, Mo, Ni, Se, Zn) elements in the tissues of cattle from NW Spain. Biometals, 17(4), 389–397. https://doi.org/10.1023/B:BIOM.0000029434.89679.a2
López-Alonso, M. (2012). Trace minerals and livestock: Not too much not too little. ISRN Veterinary Science, 2012, Article 704825. https://doi.org/10.5402/2012/704825
Miranda, M., Alonso, M. L., & Benedito, J. L. (2007). Copper, zinc, iron, and manganese accumulation in cattle from Asturias (northern Spain). Biological Trace Element Research, 109(2), 135–149. https://doi.org/10.1385/BTER:109:2:135
Nishikawa, H., Asai, A., & Fukunishi, S. (2022). The significance of zinc in patients with chronic liver disease. Nutrients, 14(22), Article 4855. https://doi.org/10.3390/nu14224855
Ogbomida, E. T., Nakayama, S. M. M., Bortey-Sam, N., Oroszlany, B., Tongo, I., Enuneku, A. A., Ozekeke, O., Ainerua, M. O., Fasipe, O., Rasaki, A., Ogbomida, A., & Ikenaka, Y. (2018). Accumulation patterns and risk assessment of metals and metalloid in muscle and offal of free-range chickens, cattle and goat in Benin City, Nigeria. Ecotoxicology and Environmental Safety, 151, 98–108. https://doi.org/10.1016/j.ecoenv.2018.01.008
Philippe, M. A., Ruddell, R. G., & Ramm, G. A. (2007). Role of iron in hepatic fibrosis: One piece in the puzzle. World Journal of Gastroenterology, 13(35), 4746–4754. https://doi.org/10.3748/wjg.v13.i35.4746
Riska, Tasabaramo, I. A., Zamani, N. P., Lalang, & Syadiah, E. A. (2022). Akumulasi logam berat (Pb, Cd, Hg) pada karang Acropora aspera di Perairan Pomalaa Sulawesi Tenggara. Jurnal Ilmu dan Teknologi Kelautan Tropis, 14(1), 69–80. https://doi.org/10.29244/jitkt.v14i1.37553
Singare, P. B., Patil, V. D., & Pillewad, S. R. (2018). Soil-plant-animal relationship of micro minerals in cattle from northern tehsils of Parbhani District, India. International Journal of Current Microbiology and Applied Sciences, 7(6), 1105–1110. https://doi.org/10.20546/ijcmas.2018.706.130
Sohal, A., & Kowdley, K. V. (2024). A review of new concepts in iron overload. Gastroenterology & Hepatology, 20(2), 98–107. https://pmc.ncbi.nlm.nih.gov/articles/PMC10895914/
Tahir, I., & Alkheraije, K. A. (2023). A review of important heavy metals toxicity with special emphasis on nephrotoxicity and its management in cattle. Frontiers in Veterinary Science, 10, Article 1149720. https://doi.org/10.3389/fvets.2023.1149720
Ultra, V. U., Manyiwa, T., Rantong, G., Opaletswe, K. A., Gabankitse, G., Taupedi, S. B., & Gajaje, K. (2021). Heavy metals in soil, plants, and associated risk on grazing ruminants in the vicinity of Cu–Ni mine in Selebi-Phikwe, Botswana. Environmental Geochemistry and Health, 44, 1633–1648. https://doi.org/10.1007/s10653-021-00918-x
Wysocka, D., Snarska, A., & Sobiech, P. (2020). Iron in cattle health: Review paper. Journal of Elementology, 25(3), 1175–1185. https://doi.org/10.5601/jelem.2020.25.2.1960
Yabe, J., Nakayama, S. M. M., Ikenaka, Y., Muzandu, K., Ishizuka, M., & Umemura, T. (2012). Accumulation of metals in the liver and kidneys of cattle from agricultural areas in Lusaka, Zambia. Journal of Veterinary Medical Science, 74(10), 1345–1347. https://doi.org/10.1292/jvms.12-0142
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