Vol. 26 No. 2 (2026): April - Juni
Open Access
Peer Reviewed

Chlorophyll Response in Ipomoea reptans Leaves to Liquid Organic Fertilizer Application from Vegetable Waste

Authors

Mega Nofitri Ikhwan , Prapti Sedijani , Rubiyatna Sakaroni

DOI:

10.29303/jbt.v26i2.11905

Published:

2026-05-22

Downloads

Abstract

Modern agricultural practices that use synthetic inorganic fertilizers excessively and uncontrolled can disrupt the soil’s ecological balance, reduce microbial activity, and disrupt plant physiological processes, so that the development of alternative, environmentally friendly fertilizers is needed. This study aimed to evaluate the physiological response of Ipomoea reptans to the application of LOF derived from vegetable waste, using leaf chlorophyll content and vegetative growth parameters as indicators. The experiment was conducted using a Completely Randomized Design (CRD) with treatments consisting of combinations of NPK fertilizer and LOF at different concentrations. The observed parameters included the number of leaves, leaf area, and chlorophyll content, and the data were analyzed using One-Way ANOVA followed by Tukey’s test and Pearson correlation analysis. The results showed that the application vegetable-waste LOF tended to enhance the vegetative growth of Ipomoea reptans, with the highest number of leaves and leaf area observed in treatment N0P3 (0 g NPK + 60 ml LOF). However, statistical analysis indicated that most treatments did not differ significantly at the 5% significance level. Chlorophyll content also did not differ significantly among treatments, although the highest value was recorded in treatment N0P2 (40 ml LOF without NPK). Pearson correlation analysis revealed a very weak and non-significant relationship between leaf number and chlorophyll content (r = −0.019; p = 0.916; n = 32) and between leaf area and chlorophyll content (r = −0.045; p = 0.807; n = 32). Based on data, there is a tendency to increase vegetative growth with vegetable waste-based LOF, although statistically there is no significant difference. These findings indicate that vegetable waste-based LOF has the potential to be developed as an environmentally friendly alternative fertilizer to support sustainable cultivation and improve understanding of plant physiological responses during vegetative growth.

Keywords:

Chlorophyll content Ipomoea reptans Liquid organic fertilizer Vegetable waste

References

Adisa, V. (2023). Panduan lengkap budi daya kangkung (Edisi pertama). Pustaka Referensi.

Afner, S. O. G., Utami, K., Sari, D. P., Siregar, A., Jamilah, J., Syofiani, R., Saida, S., Yuniarti, A., Yamin, B. M., Mulyani, S., Kartini, N. L., & Suparwata, D. O. (2024). Kesuburan tanah dan pemupukan (Cetakan pertama). CV HEI Publishing Indonesia.

Afrijal, A., Syuhada, F. A., Jarlis, R., & Hendrita, V. (2024). Teknik budidaya tanaman kangkung darat (Ipomoea reptans Poir) secara organik di CV. Faruq Farm. Agriness, 2(1). https://doi.org/10.55296/agriness.v2i1.52

Agustin, R., Wahyuni, S., & Nurhayati, N. (2023). Pemanfaatan effective microorganism 4 (EM4) dalam pembuatan pupuk organik cair untuk meningkatkan kesuburan tanah dan pertumbuhan tanaman. Jurnal Ilmu Pertanian Indonesia, 28(2), 145–153. https://doi.org/10.24843/AJoAS.2022.v12.i02.p02

Aranda, N. P., Santoso, B. B., Muthahanas, I., & Rahayu, S. (2023). Pengaruh pemberian pupuk organik cair (POC) limbah cair tahu terhadap pertumbuhan dan hasil tanaman sawi (Brassica juncea L.). Jurnal Ilmiah Mahasiswa Agrokomplek, 2(1), 37–44. https://doi.org/10.29303/jima.v2i1.2289

Ardiyanti, D., Fahriah, S. Y., & Chodijah, M. (2021). Pemanfaatan limbah sayur sebagai pupuk organik cair tanaman di RW 12 Kelurahan Babakan Surabaya. Proceedings UIN Sunan Gunung Djati Bandung, 1(15), 123–133. https://proceedings.uinsgd.ac.id/index.php/Proceedings

Aryani, M., Raksun, A., & Mertha, I. G. (2024). The effect of using NPK fertilizer and liquid organic fertilizer vegetable waste on the vegetative growth of purple eggplant (Solanum melongena L.). Jurnal Biologi Tropis, 24(2), 812–824. https://doi.org/10.29303/jbt.v24i2.6973

Burhan, A. (2022). Respon pertumbuhan tanaman kangkung darat (Ipomoea reptans Poir) terhadap pemberian pupuk organik di lahan sawah Desa Kelondom. Jurnal Inovasi Penelitian, 2(12), 4211–4218. https://doi.org/10.47492/jip.v2i12.1525

Chairiyah, N., Murtilaksono, A., Adiwena, M., & Fratama, R. (2022). Pengaruh dosis pupuk NPK terhadap pertumbuhan vegetatif tanaman cabai rawit (Capsicum frutescens L.) di tanah marginal. Jurnal Ilmiah Respati, 13(1), 1–9. http://ejournal.urindo.ac.id/index.php/pertanian/article/view/2197

Chamidah, A., Afrilia, H. C., Ahmad, M. G., & Arisandi, D. (2024). Isolasi klorofil a dan analisis aktivitas antioksidan dari mikroalga Chlorella vulgaris. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(11), 1006–1020. https://doi.org/10.17844/jphpi.v27i11.57470

Dewi, S. M., Harahap, L. H., Arisandi DN, D., & Alpandari, H. (2024). Pertanian budidaya dan tanaman (Cetakan pertama). PT Literasi Nusantara Abadi Grup.

Fauzan, A., & Sitawati. (2022). Pengaruh penggunaan PGPR dan NPK terhadap fase vegetatif dan generatif pada tanaman bunga marigold (Tagetes erecta L.). Jurnal Produksi Tanaman, 10(11), 596–603. https://doi.org/10.21776/ub.protan.2022.010.11.01

Febbriana, W., Yakop, U. M., & Suryaningsih B. L. (2024). Respon pertumbuhan kangkung darat (Ipomoea reptans Poir) terhadap pemberian berbagai dosis pupuk kascing: Growth response of land spinach (Ipomoea reptans Poir) to various doses of vermicompost fertilizer. Jurnal Ilmiah Mahasiswa Agrokomplek, 3(2), 148–155. https://doi.org/10.29303/jima.v3i2.5323

Food and Agriculture Organization. (2022). The state of food and agriculture 2022. FAO.

Govindjee, G., Stirbet, A., Lindsey, J. S., & Scheer, H. (2024). On the Pelletier and Caventou (1817, 1818) papers on chlorophyll and beyond. Photosynthesis Research, 151(1), 1–25. https://doi.org/10.1007/s11120-024-01081-x

Hamzah, A., & Siswanto, B. (2023). Pupuk organik: Tinjauan teori & praktek (Edisi pertama). Forind.

Haryanta, D., Tojibatus Sa’adah, I. T., & Thohiron, M. (2021). Pembuatan pupuk organik cair (POC) berbahan baku limbah organik perkotaan dengan metode D’Wijaya. UWKS Press.

Kusumawati, A. (2021). Kesuburan tanah dan pemupukan (Edisi pertama). Poltek LPP Press.

Liu, X., Zhang, Y., Wang, Y., Li, Z., Zhao, J., & Chen, F. (2023). Long-term chemical fertilization reduces soil aggregate stability and soil organic carbon in agricultural soils. Frontiers in Plant Science, 14, 1126150. https://doi.org/10.3389/fpls.2023.1126150

Masliani, M., Mahrita, S., Sari, M., & Lestari, Y. M. (2024). Pertanian era modern: Dinamika pertanian dan solusi inovatif untuk petani. PT Media Penerbit Indonesia.

Mendrofa, M. T., & Gulo, D. (2024). Pengaruh pupuk organik terhadap perbaikan struktur dan stabilitas tanah. PENARIK: Jurnal Ilmu Pertanian dan Perikanan, 1(1), 105–110. https://doi.org/10.70134/penarik.v2i2.72

Ningsih, M. S., Susilo, E., Rahmadina, R., Qolby, F. H., Tanjung, D. D., Anis, U., Susila, E. N., Panggabean, N. H., Priyadi, S., Nasution, J., Sari, N. Y., Baharuddin, R., & Wisnubroto, M. P. (2024). Dasar-dasar fisiologi tumbuhan (Cetakan pertama). CV HEI Publishing Indonesia.

Paiman, P. A. (2022). Pertumbuhan dan perkembangan tanaman. UPY Press.

Purba, T., Situmeang, R., Rohman, H. F., Mahyati, A., Firgiyanto, R., Junaedi, A. S., Saadah, T. T., Junairiah, Herawati, J., & Suhastyo, A. A. (2021). Pupuk dan teknologi pemupukan (R. Watrianthos, Ed.). Yayasan Kita Menulis.

Raksun, A., Ilhamdi, L., Merta, I. W., & Mertha, I. G. (2020). Vegetative growth of pakcoy (Brassica rapa L.) due to different dose of bokashi and NPK fertilizer. Jurnal Biologi Tropis, 20(3), 452–459. https://doi.org/10.29303/jbt.v20i3.2156

Rantung, R. C., Supit, P. C. H., Paulus, J. M., Rantung, M. R., Wanget, S. A., & Inkiriwang, A. E. B. (2023). Pengaruh pemberian pupuk organik cair limbah dapur terhadap pertumbuhan dan hasil tanaman kangkung darat (Ipomoea reptans Poir). Eugenia, 30(3), 1–12. https://doi.org/10.35791/eug.v30i3.60642

Rozari, R., Kisman, & Jayaputra. (2023). Pengaruh pemberian pupuk cair Bioextrim terhadap pertumbuhan dan hasil dua varietas bawang merah (Allium ascalonicum L.). Jurnal Ilmiah Mahasiswa Agrokomplek, 2(3), 321–328. https://doi.org/10.29303/jima.v2i3.3558

Salim, T., Tarigan, D. M., & Haireen, R. M. R. (2025). Pengaruh pemberian pupuk NPK 15:15:15 terhadap pertumbuhan tanaman selasih (Ocimum basilicum), mint (Mentha spp.), dan sambung nyawa (Gynura procumbens). Jurnal Agrotek, 9(1), 66–81. http://ejurnal.umsu.ac.id/index.php/agrotek/article/view/713

Sahir, S. H. (2021). Metodologi penelitian (Cetakan pertama). Penerbit KBM Indonesia.

Septiadi, A., & Ramadhani, W. K. (2020). Penerapan metode ANOVA untuk analisis rata-rata produksi donat, burger, dan croissant pada Toko Roti Animo Bakery. Bulletin of Applied Industrial Engineering Theory, 1(2), 60–64. https://doi.org/10.47492/jip.v2i12.1525

Sondang, Y., Elita, N., & Anidarfi. (2020). Buku ajar praktek: Fisiologi tanaman (Cetakan pertama). Politeknik Pertanian Negeri Payakumbuh.

Suwignya, I. A., Pelealu, J. J., & Tallei, T. E. (2024). Pengaruh penambahan pupuk organik dan mikoriza terhadap kadar klorofil dan antosianin daun ubi jalar ungu (Ipomoea batatas var. Ayamurasaki). Jurnal BIOS LOGOS, 13(3). https://doi.org/10.35799/jbl.v13i3.49247

Widowati, L. R., Hartatik, W., Setyorini, D., & Trisnawati, Y. (2022). Pupuk organik: Dibuatnya mudah, hasil tanam melimpah. Pusat Perpustakaan dan Penyebaran Teknologi Pertanian, Kementerian Pertanian Republik Indonesia.

Author Biographies

Mega Nofitri Ikhwan, Universitas Mataram

Author Origin : Indonesia

Prapti Sedijani, Universitas Mataram

Author Origin : Indonesia

Rubiyatna Sakaroni, Universitas Mataram

Author Origin : Indonesia

Downloads

Download data is not yet available.

How to Cite

Ikhwan, M. N., Sedijani, P., & Sakaroni, R. (2026). Chlorophyll Response in Ipomoea reptans Leaves to Liquid Organic Fertilizer Application from Vegetable Waste. Jurnal Biologi Tropis, 26(2), 843–854. https://doi.org/10.29303/jbt.v26i2.11905

Most read articles by the same author(s)

1 2 3 4 5 > >> 

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.