Effects of Nano Fern Extract (Diplazium esculentum) and Humic Acid on Chlorophyll Content of Soybean (Glycine max)
DOI:
10.29303/jbt.v26i3.11918Published:
2026-07-05Downloads
Abstract
As the population continues to increase, the demand for soybeans in Indonesia is rising, necessitating effective strategies to enhance soybean growth and production. One such strategy is the use of biostimulants, including nano-fern extract and humic acid. This study aimed to evaluate the effects of different concentrations of nano-fern extract and humic acid, as well as their interaction, on the chlorophyll content and flowering time of soybeans. The experiment was conducted using a Factorial Completely Randomized Design (CRD) with two factors and six replications. Factor A (fern extract) consisted of a control, 100 mg/L of crude fern extract, and nano-fern extract at concentrations of 50, 100, and 150 mg/L. Factor B (humic acid) consisted of a control, 1200 mg/L, and 2500 mg/L. The results showed that the application of nano-fern extract at 150 mg/L and humic acid at 2500 mg/L resulted in higher chlorophyll content than other treatments.
Keywords:
Biostimulant Concentration Fern extract Humic acid SoybeanReferences
Adisti, J. P., Suwirmen & Idris, M. (2023). Pengaruh Ekstrak Pegagan (Centella asiatica (L.) Ubr.) Dengan Beberapa Jenis Pelarut Sebagai Biostimulan Terhadap Pertumbuhan Sawi pagoda (Brassica rapa var. narinosa L.). Jurnal Biologi Universitas Andalas, 11(1), 54-61. https://doi.org/10.25077/jbioua.11.1.54-61.2023
Ayumi, T. S., Zakiah, Z. & Linda, R. (2023). Potensi Ekstrak Daun Kirinyuh (Chromolaena odorata L.) Sebagai Biostimulan Terhadap Perkecambahan Kacang Tanah (Arachis hypogaea L.). Protobiont, 12(1), 28-33. https://doi.org/10.26418/protobiont.v12i1.68666
Dharmadewi, A. A. I. M. (2020). Analisis Kandungan Klorofil Beberapa Jenis Sayuran Hijau Sebagai Alternatif Bahan Dasar Food Suplement. Jurnal Emasains, 9(2), 171-176. https://doi.org/10.5281/zenodo.4299383
Dilla, A. I. (2024). Pengaruh Ekstrak Kasar Beberapa Jenis Paku Sebagai Biostimulan Terhadap Pertumbuhan dan Hasil Tanaman Kedelai (Glycine max (L.) Merr.). Tesis, Universitas Andalas. Universitas Andalas
Du Juardin, P. (2015). Plant Biostimulan: Definition, Concept, Main Categories and Regulation. Scientia Horticulturae, 196, 3-14. https://doi.org/10.1016/j.scienta.2015.09.021
Magnabosco, P., Masi, A., Shukla, R., Bansal, V. & Carletti, P. (2023). Advancing the impact of plant biostimulants to sustainable agriculture through nanotechnologies. Chemical and Biological Technologies in Agriculture, 10(117), 1-25. https://doi.org/10.1186/s40538-023-00491-8
Mollah, A., Iswoyo, H. & Reskiana, N. (2020). Application of humic acid and guano on sugarcane seedlings with bud stepropagation method. IOP Conf. Series: Earth and Environmental Sciences, 575(012193), 1-7. https://doi.org/10.1088/1755-1315/575/1/012193
Noli, Z. A. & Labukti, H. V. (2020). Pengaruh Ekstrak Paku Resam (Gleichenia linearis) sebagai Biostimulan terhadap Pertumbuhan dan Hasil Cabai Keriting (Capsicum annum L.) Kultivar Kopay. Agro Bali Agricultural Journal, 3(5), 492-497. https://doi.org/10.37637/ab.v5i3.999
Paelongan, A. H., Malau, K. M. & Semahu, L. H. (2023). Pengaruh Ekstrak Bawang Merah (Allium cepa L.) sebagai Zat Pengatur Tumbuh pada Benih Kakao (Theobroma cacao L.). Jurnal Agro Industri Perkebunan, 11(3), 185-196. https://doi.org/10.25181/jaip.v11i3.3013
Rahmandhias, D. T. & Rachmawati, D. (2020). Pengaruh Asam Humat terhadap Produktivitas dan Serapan Nitrogen pada Tanaman Kangkung Darat (Ipomoea reptans Poir.). Jurnal Ilmu Pertanian Indonesia, 25(2), 316-322. http://journal.ipb.ac.id/index.php/JIPI.
Rasyidi, A. F., Sulistiani, R. & Jalani, S. I. B. (2024). Kadar Klorofil Daun Bibit Kelor (Moringa oleifera L.) Pada Berbagai Dosis Kompos. Agrium, 27(1), 33-43. https://doi.org/10.30596/agrium.v27i1.17486
Shayen, M. P., Noli, Z. A., Maideliza, T. dan Suwirmen. (2023). Pengaruh Aplikasi Nanobiostimulan Rumput Laut (Padina minor Yamada) Terhadap Kadar Klorofil Kedelai (Glycine max (L.) Merr.). Bioscientist, 11(2), 1176-1185. https://doi.org/10.33394/bioscientist.v11i2.9063
Singh, A., Singh, N. B., Hussain, I., Singh, H. & Singh, S. C. (2015). Plant-nanoparticle Interaction: An Approach to Improve Agricultural Practices and Plant Productivity. International Journal of Pharmaceutical Science Invention, 4(8), 25-40. https://www.ijpsi.org/Papers/Vol4(8)/E0480025040.pdf
Van Oosten, M. J., Pepe, O., De Pascale, S., Silleti, S. dan Maggio, A. (2017). The role of biostimulants and bioeffectors as alleviators of abiotic stress in crop plants. Chemical and Biological Technologies in Agriculture, 4(5), 1-12. https://doi.org/10.1186/s40538-017-0089-5
Wahyuni, A., Zakiah, Z. & Mukarlina. (2023). Pertumbuhan Sawi pakchoy (Brassica rapa L.) pada Media Hidroponik Sistem sumbu Setelah Pemberian Biostimulan Ekstrak Pegagan (Centella asiatica L.). Protobiont, 12(1),14-21. https://doi.org/10.26418/protobiont.v12i1.66752
License
Copyright (c) 2026 Tita Nopiyanti, Zozy Aneloi Noli, M. Idris
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
