The Effect of Ciu Alcohol on MCV and Neutrophils in White Rats (Rattus norvegicus)
DOI:
10.29303/jbt.v26i2.12063Published:
2026-06-16Downloads
Abstract
Alcohol consumption is a public health problem that is still a concern in various countries. One type of alcoholic beverage commonly found in the Sukoharjo area is ciu. This study aims to determine the effect of giving traditional alcohol ciu on the Mean Corpuscular Volume (MCV) value and the number of neutrophils in male white rats (Rattus norvegicus). The study used a quasy-experiment/post test design with 27 male white rats of the Wistar strain divided into three groups, namely the 0 ml ciu control group, the 2 ml ciu treatment group, and the 4 ml ciu treatment group for 14 days. Then the MCV value was examined using the Abacus 3CT Hematology Analyzer and the number of neutrophils was calculated using the diff count method. Then, the data were analyzed using the Shapiro-Wilk test, Levene's test, and One-Way ANOVA test. The outcomes revealed that there were normal and less than normal MCV values and there was an increase in the number of neutrophils. The conclusion of this investgation is that there is no effect of giving traditional ciu alcohol to male white rats (Rattus norvegicus) on MCV values and neutrophil counts.
Keywords:
Neutrophil Count Mean Corpuscular Volume Traditional Ciu Alcohol White Rat (Rattus norvegicus)References
Acat, O., & Imre, O. (2025). Inflammatory biomarkers in alcohol use disorder:neutrophil/lymphocyte and monocyte/HDL-C ratios as potential diagnostic indicators. European Journal of Medical Research, 30(1). https://doi.org/10.1186/s40001-025-03262-3
Adeleye, G. S., Odesanmi, E. O., Ajeigbe, K., Omayone, T., Odetola, A., Awoyinka, A. O., ... & Adeyeye, V. J. (2023). Effects of Ethanol on Hematological, Histopathological, and Antioxidant on Indomethacin-induced Heat-stressed Rats. Int J Pharm Biol Arch. 14(3), 119–122. www.ijpba.in
Aroca-Crevillén, A., Adrover, J. M., & Hidalgo, A. (2020). Circadian Features of Neutrophil Biology. Frontiers in Immunology, 11(April),19. https://doi.org/10.3389/fimmu.2020.0057
Ballesteros, I., Rubio-Ponce, A., Genua, M., Lusito, E., Kwok, I., Fernández-Calvo, G., Khoyratty, T. E., van Grinsven, E., González-Hernández, S., Nicolás-Ávila, J. Á., Vicanolo, T., Maccataio, A., Benguría, A., Li, J. L. Y., Adrover, J. M., Aroca-Crevillen, A., Quintana, J. A., Martín-Salamanca, S., Mayo, F., Hidalgo, A. (2020). Co-option of Neutrophil Fates by Tissue Environments. Cell, 183(5), 1282-1297.e18. https://doi.org/10.1016/j.cell.2020.10.0
Crotty, K., Anton, P., Coleman, L. G., Morris, N. L., Lewis, S. A., Samuelson, D.R., McMahan, R. H., Hartmann, P., Kim, A., Ratna, A., Mandrekar, P., Wyatt,T. A., Choudhry, M. A., Kovacs, E. J., McCullough, R., & Yeligar, S. M.(2023). A critical review of recent knowledge of alcohol’s effects on theimmunological response in different tissues. Alcohol: Clinical and Experimental Research, 47(1), 36–44. https://doi.org/10.1111/acer.14979
Djami, S. W., St, S., & Kes, M. (2025). Hematologi Teknologi Laboratorium Medis.
Ghaffari, S. (2008). Oxidative stress in the regulation of normal and neoplastic hematopoiesis. Antioxidants & redox signaling, 10(11), 1923-1940. https://doi.org/10.1089/ars.2008.2142
Giknis, M. L. A., Charles, B., & Clifford, D. V. M. (2006). Clinical LaboratoryParameters for Crl:CD(SD) Rats. Charles River Laboratories, 1–18. https://www.criver.com/sites/default/files/Technical Resources/ClinicalLaboratory Parameters for Crl-CD_SD_ Rats - March 2006.pdf
Handajani, F. (2017). Metode Pemilihan Dan Pembuatan : Hewan Model Beberapa Penyakit. In Angewandte Chemie International Edition, 6(11), 951–952. (Issue November).
Hartono, R., Soewono, S., & Ratnaningsih, T. (2019). Pengaruh Pemberian Alkohol Peroral Terhadap Nilai Mean Corpuscular Volume, Morfologi Eritrosit Darah Tepi dan Normoblas Sumsum Tulang. Jaringan Laboratorium Medis, 1(1), 46. https://doi.org/10.31983/jlm.v1i1.5161
Indonesian Ministry Of Health Development Policy Board. (2023). Indonesian Health Survey (Survei Kesehatan Indonesia) 2023. Ministry of Health, 1–68.
Ismail, S. (2022). Pengaruh Penggunaan Model Pembelajaran Berbasis Proyek “Project Based Learning” Terhadap Hasil Belajar Fisika Peserta Didik Kelas X IPA SMA Negeri 35 Halmahera Selatan Pada Konsep Gerak Lurus”. Jurnal Ilmiah Wahana Pendidikan, https://doi.org/10.5281/zenodo.6466594
Jin, L., Zhang, Z., Pan, P., Zhao, Y., Zhou, M., Liu, L., Zhai, Y., Wang, H., Xu, L.,Mei, D., Zhang, H., Yang, Y., Hua, J., Zhang, X., & Zhang, L. (2023). Low-dose ethanol consumption inhibits neutrophil extracellular traps formation to alleviate rheumatoid arthritis. Communications Biology, 6(1), 1088. https://doi.org/10.1038/s42003-023-05473-y
Khabibi, F. M. (2024). Analysis of Raw Materials and Production Process of Broiler Feed Crumble Type B-BR 1 MGB: Analisis Bahan Baku dan Proses Produksi Pakan Ternak Ayam Ras Pedaging (Broiler) Jenis Crumble Tipe B-BR 1 MGB. Procedia of Engineering and Life Science, 5, 438-445.
Klein, samuel. (2019). Akses Publik HHS. HHS Public Access, 00585702(317), 1–13. https://doi.org/10.1111/imr.12446.Patobiologi
Laeto, A. Bin, Inggarsih, R., Purnamasari, S., Diba, M. F., & Taharu, F. I. (2022). Analisis Profil Eritrosit Tikus Putih (Rattus norvegicus) Pasca Diet Vegetarian. Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton, 8(1), 107–118. https://doi.org/10.35326/pencerah.v8i1.1901
Lahamendu, B., Bodhi, W., & Siampa, J. P. (2019). Uji Efek Analgetik Ekstrak Etanol Rimpang Jahe Putih (Zingiber officinale Rosc.var. Amarum) Pada Tikus Putih Jantan Galur Wistar (Rattus norvegicus). Pharmacon, 8(4), 927. https://doi.org/10.35799/pha.8.2019.29372
Lodish, H., Flygare, J., & Chou, S. (2010). From stem cell to erythroblast: regulation of red cell production at multiple levels by multiple hormones. IUBMB life, 62(7), 492-496. https://doi.org/10.1002/iub.322
Maiese, K., Chong, Z. Z., Hou, J., & Shang, Y. C. (2008). Erythropoietin and oxidative stress. Current Neurovascular Research, 5(2), 125-142.
Malenica, M., Prnjavorac, B., Bego, T., Dujic, T., Semiz, S., Skrbo, S., Gusic, A., Hadzic, A., & Causevic, A. (2017). Effect of Cigarette Smoking on Haematological Parameters in Healthy Population. Medical Archives, 71(2), 132. https://doi.org/10.5455/medarh.2017.71.132-136
Paulson, R. F., Ruan, B., Hao, S., & Chen, Y. (2020). Stress erythropoiesis is a key inflammatory response. Cells, 9(3), 634.
Putra, A. L., Kasdi, A., & Subroto, W. T. (2019). Pengaruh Media Google Earth Terhadap Hasil Belajar Berdasarkan Keaktifan Siswa Kelas IV Tema Indahnya Negeriku di Sekolah Dasar. Jurnal Review Pendidikan Dasar: Jurnal Kajian Pendidikan Dan Hasil Penelitian, 5(3), 1034-1042. https://doi.org/10.26740/jrpd.v5n3.p1034-1042
Quraishi, R., Jain, R., & Ambekar, A. (2016). Hematological Profile of Alcohol Dependent subjects: Report from a Tertiary Care Treatment Centre in India. International Journal of Pharma Research and Health Sciences, 4(5), 1420 1423. https://doi.org/10.21276/ijprhs.2016.05.15
Rojas, D. A., Coronado, K., Pérez-Reytor, D., & Karahanian, E. (2024). Reduction of Alcohol-Dependent Lung Pathological Features in Rats Treated with Fenofibrate. International Journal of Molecular Sciences, 25(23), 1–13. https://doi.org/10.3390/ijms252312814
Rosita, L., Pramana, A. A. C., & F. R. Arfira. 2019. Hematologi Dasar. Universitas Islam Indonesia, Yogyakarta
Roychowdhury, S., Pant, B., Cross, E., Scheraga, R., & Vachharajani, V. (2024). Effect of ethanol exposure on innate immune response in sepsis. Journal of Leukocyte Biology, 115(6), 1029–1041. https://doi.org/10.1093/jleuko/qiad156
Stadlbauer, V., Horvath, A., Komarova, I., Schmerboeck, B., Feldbacher, N., Wurm, S., Klymiuk, I., Durdevic, M., Rainer, F., Blesl, A., Stryeck, S., Madl, T., Stiegler, P., & Leber, B. (2019). A single alcohol binge impacts on neutrophil function without changes in gut barrier function and gut microbiome composition in healthy volunteers. PLoS ONE, 14(2), 1–11. https://doi.org/10.1371/journal.pone.0211703
Suryanah, S., Widjaja, N., Akhdiat, T., & Permana, H. (2023). Pengaruh waktu penggantian ransum BR1 dengan BR2 terhadap performa broiler The effect of time to replace BR1 with BR2 ration on broiler performance. 5(1), 25–30
Szabo, G., & Saha, B. (2015). Alcohol’s Effect on Host Defense. Alcohol Research : Current Reviews, 37(2), 159–170. https://doi.org/10.35946/arcr.v37.2.01
Weiss, D.J., dan Wardrop K.J. 2010. Schalm’s Veterinary Hematology 6th Edition. Willey-Blackwell, USA. Halaman 78-84, 152-161.
Zhu, W., Fan, C., Dong, S., Li, X., Chen, H., & Zhou, W. (2023). Neutrophil extracellular traps regulating tumorimmunity in hepatocellular carcinoma. Frontiers in Immunology, 14(December), 1–18. https://doi.org/10.3389/fimmu.2023.1253964
Zonyfar, C., Nuraini, R., Purnia, D. S., Setyawati, I., Evi, T., Dian, S., Permana, H., & Sumartiningsih, M. S. (2022). Buku Metodologi Penelitian.
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