Effect of Suji Leaf Ethanolic Extract (Dracaena angustifolia) on The Sperm Motility of Mice (Mus musculus) Exposed to Cigarette Smoke
DOI:
10.29303/jbt.v26i2.12078Published:
2026-06-12Downloads
Abstract
Cigarette smoke exposure can induce oxidative stress, leading to reduced sperm motility. Suji leaves contain antioxidants, including flavonoids, alkaloids, terpenoids, and steroids. This study aims to determine the effect of cigarette smoke exposure on mouse sperm motility and the effect of suji leaf ethanolic extract on the sperm motility of mice exposed to cigarette smoke. This study employed an experimental research design using a factorial Completely Randomized Design (CRD) involving 48 mice. Two factors were combined in the treatment groups: the duration of cigarette smoke exposure (0, 5, 15, and 30 minutes) and the dose of suji leaf ethanolic extract (0, 250, 500, and 750 ppm). Sperm motility was assessed using a hemocytometer under a light microscope at 400× magnification. Data were analyzed using a two-way analysis of variance. The results of statistical analysis indicated that cigarette smoke exposure had a significant effect on mouse sperm motility (0.000 < 0.05), and that suji leaf ethanolic extract significantly affected the sperm motility of mice exposed to cigarette smoke (0.036 < 0.05). These results indicate that cigarette smoke exposure can reduce sperm motility, and the administration of suji leaf ethanolic extract is able to increase and improve the sperm motility of mice caused by cigarette smoke exposure. Thus, suji leaf ethanolic extract can serve as a natural antioxidant to improve sperm motility affected by cigarette smoke exposure.
Keywords:
Cigarette smoke Mice Sperm motility Suji leavesReferences
Agarwal, A., Virk, G., Ong, C., & Du Plessis, S. S. (2014). Effect of oxidative stress on male reproduction. The world journal of men's health, 32(1), 1. DOI: https://doi.org/10.5534/wjmh.2014.32.1.1
Amalo, D., Mauboy, R. S., Karyawati, A., Ruma, M. T. L., Dima, A. O. M., & Saminda, F. R. (2022). Aktivitas ekstrak daun suji (Dracaena angustifolia) terhadap daya hambat pertumbuhan jamur Candida albicans. Jurnal Biotropikal Sains, 19(3), 77-82.
Amelia, S. P., Sopiah, P., & Ridwan, H. (2023). Hubungan patologi dan patofisiologi pada individu akibat normalisasi perilaku merokok di Indonesia. Jurnal Keperawatan Abdurrab, 7(1), 23-28. DOI: https://doi.org/10.36341/jka.v7i1.3360
Arifin, B., & Ibrahim, S. (2018). Struktur, bioaktivitas dan antioksidan flavonoid. Jurnal zarah, 6(1), 21-29. DOI: https://doi.org/10.31629/zarah.v6i1.313
Bahri, S., Setiadi, D., Lestari, T. A., Setiawan, H., Hakim, M. A., & Cahyadi, L. M. Z. (2025). Phytochemical screening of suji (Pleomele angustifolia) extract. Jurnal Biologi Tropis, 25(2), 2395-2402. DOI: https://doi.org/10.29303/jbt.v25i2.9560
Bahri, S., Setiadi, D., Lestari, T. A., Setiawan, H., Hakim, M. A., Cahyadi, L. M. Z. (2025). The comparison of ic50 of suji (Pleomele angustifolia) which is extracted by different solvent. Jurnal Biologi Tropis, 25(2), 2403-2409. DOI: https://doi.org/10.29303/jbt.v25i2.9559
Batubara, I. V. D., Wantouw, B., & Tendean, L. (2013). Pengaruh paparan asap rokok kretek terhadap kualitas spermatozoa mencit jantan (Mus musculus). eBiomedik, 1(1), 330-337. DOI: https://doi.org/10.35790/ebm.1.1.2013.4367
Bauer, K. N, & Nixon, B. (2020). Molecular changes induced by oxidative stress that impair human sperm motility. Antioxidants, 9(2), 134. https://doi.org/10.3390/antiox9020134
Desiani, E., Mardiana, T. Y., Madusari, B. D., & Hidayat, F. N. (2022). Uji aktivitas analgesik ekstrak daun mangrove (Rhizophora mucronata) pada mencit yang diinduksi asam asetat dengan metode Writhing Reflex. Cendekia Journal of Pharmacy, 6(2), 307-317. DOI: https://doi.org/10.31596/cjp.v6i2.213
Fauziah, C., Hasanah, U., Irsyad, N. S., Pribadi, I., Setiawan, R. P., & Wahyuni, Y. P. (2023). Analisis motilitas dan morfologi spermatozoa pria perokok usia 18-24 tahun. Jurnal Majalah Kedokteran Andalas, 46(1), 79-87. DOI: https://doi.org/10.25077/mka.v46.i1.p79-87.2023
Guiekep, A. J. N., Kenfack, A., Ngoula, F., Vemo, B. N., Nguemmeugne, K. S., & Tedonkeng, E. P. (2019). Attenuating effects of Mangifera indica leaves ethanolic extract against acetamiprid induced reproductive toxicity in male guinea pigs. Veterinary Research Forum, 10(3), 187-192. DOI: https://doi.org/10.30466/vrf.2019.95154.2292
Gusnirwan, A., & Sangging, P. R. A. (2024). Tinjauan: malondialdehyde (MDA) sebagai marker stres oksidatif berbagai penyakit. Medical Profession Journal of Lampung, 14(2), 321-325. DOI: https://doi.org/10.53089/medula.v14i2.990
Hasmar, W. N., Pratiwi, I. S., Feriadi, E., Yunita, A., Yusuf, M., Ardiansyah, A., Kamaruddin, H. S., & Masdin, M. R. (2025). Farmakologi untuk farmasi. Kendari: Progres Ilmiah Kesehatan. ISBN: 978-634-04-2395-2
Hikmah, F., Hardiany, N. S., & Kunci, K. (2021). The role of reactive oxygen species (ros) in cancer stem cells. Jurnal Kedokteran Yarsi, 29(3), 120-134. DOI: https://doi.org/10.33476/jky.v29i3.1270
Ilmi, M. Y. M., Bahri, S., & Lestari, T. A. (2025). Effect of Ethanolic Extract of Ketip Banana (Musa paradisiaca Forma Typiaca) Peel on Sperm Morhology in Mice (Mus musculus) Exposed to Cigarette Smoke. Jurnal Biologi Tropis, 25(4a), 761-766. DOI: https://doi.org/10.29303/jbt.v25i4a.11104
Julaecha, J., & Wuryandari, A. G. (2021). Pengetahuan dan sikap tentang perilaku merokok pada remaja. Jurnal Akademika Baiturrahim Jambi, 10(2), 313-318. DOI: https://doi.org/10.36565/jab.v10i2.337
Kasmeri, R., & Putri, A. (2020). Pengaruh ekstrak pedada merah (Sonneratia caeolaris l.) terhadap jumlah dan morfologi spermatozoa mencit (Mus musculus L.). Jurnal Biologi dan Pendidikan Biologi, 6(1), 7-13. DOI: http://dx.doi.org/10.22202/bc.2020.v6i2.4174
Luthfi, M. J. F., & Noor, M. M. (2020). A simple technique for rapid assessment of rat (Rattus norvegicus) sperm motility. Biology, Medicine, & Natural Product Chemistry, 9(2), 105-107. DOI: https://doi.org/10.14421/biomedich.2020.92.105-107
Marieta, A., & Lestari, K. (2022). Review artikel: rokok dan bebagai masalah kesehatan yang ditimbulkannya. Farmaka, 20(2), 56-63. DOI: https://doi.org/10.24198/farmaka.v20i2.34618
Mishra, R., Nikam, A., Hiwarkar, J., Nandgude, T., Bayas, J., & Polshettiwar, S. (2024). Flavonoids as potential therapeutics in male reproductive disorders. Future Journal of Pharmaceutical Sciences, 10(1), 100. DOI: https://doi.org/10.1186/s43094-024-00677-3
Oviosun, E. C., Oviosun, A., Nto, N. J., & Anyanwu, E. G. (2025). Hesperidin protects against spermatological damages and testicular apoptosis induced by bisphenol-a in adult male wistar rats. Phytomedicine Plus, 5(2), 100797. DOI: https://doi.org/10.1016/j.phyplu.2025.100797
Prangdimurti, E., Muchtadi, D., Astawan, M., & Zakaria, F. R. (2006). Aktivitas antioksidan ekstrak daun suji (Pleomele angustifolia N. E. Brown). Jurnal Teknologi dan Industri Pangan, 17(2), 79-79. https://journal.ipb.ac.id/jtip/article/view/420
Putriyana, P., & Ridwanto, R. (2023). Uji aktivitas antioksidan ekstrak etanol daun suji (Dracaena angustifolia) dengan metode dpph (1,1-Difenil-2-Pikrilhidrazil). Zahra: Journal Of Health And Medical Research, 3(1), 86-97. http://repository.umnaw.ac.id/jspui/handle/123456789/2821
Rakhmawan, A., Wahyuni, E. A., & Sidik, R. F. (2022). Pengaruh kognitif siswa terhadap kemampuan interkoneksi multipel representasi siswa pada topik dinamika kimia. Natural Science Education Research, 5(1), 11-19. DOI: https://doi.org/10.21107/nser.v5i1.15318
Rakhmawan, A., Wahyuni, E. A., & Sidik, R. F. (2022). Pengaruh kognitif siswa terhadap kemampuan interkoneksi multipel representasi siswa pada topik dinamika kimia. Natural Science Education Research, 5(1), 11-19. DOI: https://doi.org/10.21107/nser.v5i1.15318
Ramadhani, R., & Bina, N. S. (2021). Statistika penelitian pendidikan: analisis perhitungan matematis dan aplikasi spss edisi pertama. Jakarta: Kencana. ISBN: 9786232189379.
Renandra, S. J., Rahmawati, Y., & Amalia, A. A. (2024). Gambaran histologi paru mencit (Mus musculus) yang dipaparkan asap rokok konvensional dan asap rokok elektrik sebagai visualisasi paru perokok pasif. Jurnal Kesehatan Tambusai, 5(4), 11576-11584. DOI: https://doi.org/10.31004/jkt.v5i4.37434
Riana, E. N., Ischak, N. I., Futri, C. L., Ayudia, E. I., Khairani, I. A., Lubis, N. A., Prabandari, A. S., Miftahurrahmah., Sari, M. S., Mulyana, J. S., & Isdaryanti. (2023). Toksikologi dasar. Bandar Lampung: Yayasan Kita Menulis. ISBN: 978-623-342-783-8
Sari, A. P., Hasanah, S., & Nursalman, M. (2024). Uji normalitas dan homogenitas dalam analisis statistik. Jurnal Pendidikan Tambusai, 8(3), 51329-51337. DOI: https://jptam.org/index.php/jptam/article/view/24059
Sholihin, A., & Ducha, N. (2024). Pengaruh kemangi (Ocimum basilicum) terhadap kualitas spermatozoa mencit (Mus musculus) yang diberikan paparan asap rokok. LenteraBio: Berkala Ilmiah Biologi, 13(2), 289-299. DOI: https://doi.org/10.26740/lenterabio.v13n2.p289-299
Simangunsong, D. K., Sulistio, S., Simbolon, E., & Simangunsong, E. K. (2024). Terapi non-invasive continuous positive airway pressure (CPAP) ventilation pada keracunan gas karbon monoksida. Cermin Dunia Kedokteran, 51(2), 76-81. DOI: https://doi.org/10.55175/cdk.v51i2.1022
Simorangkir, D. M., Meliala, L., & Wulandari, W. (2021). Formulasi dan uji efektivitas sediaan gel dari ekstrak daun suji (Dracaena agustifolia) pada tikus jantan putih sebagai antiinflamasi. Jurnal Penelitian Farmasi Dan Herbal, 4(1), 60-68. DOI: https://doi.org/10.36656/jpfh.v4i1.766
Unitly, A. J. A., Eddy, L., Nindatu, M., & Reasoa, J. (2022). Peningkatan motilitas dan viabilitas spermatozoa Rattus norvegicus terpapar asap rokok pasca diterapi sirup cengkeh. Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi, 14(1), 14-20. DOI: https://dx.doi.org/10.24815/jbe.v14i1.26345
Widiasriani, I. A. P., Udayani, N. N. W., Triansyah, G. A. P., Dewi, N. P. E. M. K., Wulandari, N. L. W. E., & Prabandari, A. A. S. S. (2024). Artikel review: peran antioksidan flavonoid dalam menghambat radikal bebas. Journal Syifa Sciences and Clinical Research, 6(2), 188-197. DOI: https://doi.org/10.37311/jsscr.v6i2.27055
Yuniarni, U., Nirmala, E., & Hazar, S. (2022). Uji aktivitas antibakteri ekstrak etanol daun suji (Draceana angustifolia (medik.) roxb.) terhadap bakteri penyebab jerawat Propionibacterium acnes dan Staphylococcus epidermidis. Jurnal Farmasi Galenika, 9(2), 102-111. DOI: https://doi.org/10.70410/jfg.v9i2.216
Zulaikhah, S. T., & Sampurna, S. (2022). Efek paparan asap rokok terhadap kadar total antioxidant capacity (TAC). Jurnal Penelitian Kesehatan Suara Forikes, 13(1), 209-213. DOI: http://dx.doi.org/10.33846/sf.v13i0.1710
Zulaikhah, V. N., Zahrania, A., Wijayadi, K. M. Z., Apriliani, N., Fatimah, N. A., Julianto, E. (2021). Evaluasi hasil edukasi masyarakat tentang bahaya kandungan dalam rokok. Indonesian Journal of Natural Science Education (IJNSE), 4(2), 510-515. DOI: https://doi.org/10.31002/ijnse.v4i2.1904
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