Evaluation of the Antimicrobial Activity of Simplicia Prepared from Wild and Cultivated Phallus indusiatus
DOI:
10.29303/jbt.v26i3.12096Published:
2026-07-05Downloads
Abstract
Phallus indusiatus is a macrofungus that has attracted attention because of its nutritional value and reported biological activities, including antimicrobial properties. This study evaluated the antimicrobial activity of wild and cultivated P. indusiatus against Staphylococcus aureus, Escherichia coli, and Candida albicans using the disc diffusion method. Wild samples were collected from Central Java, Indonesia, whereas cultivated samples were obtained from Hong Kong. Simplicia suspensions were prepared in sterile distilled water at a concentration of 100 mg/mL and tested against the selected microorganisms. No inhibition zones were observed in any treatment, indicating that the tested samples did not exhibit detectable antimicrobial activity under the conditions applied in this study. The positive control produced a mean inhibition zone of 32 mm, while no inhibition was observed in the negative control. Gene annotation data from previously generated whole-genome sequencing (WGS) of the wild sample were also reviewed to support the interpretation of the results. The selected annotated genes were mainly associated with cellular metabolism, transcriptional regulation, respiration, and metabolite transport. However, the genomic analysis was limited to a small number of annotated genes and was used only as supporting information. Overall, the findings suggest that aqueous suspensions of P. indusiatus simplicia did not show detectable antimicrobial activity against the tested microorganisms under the experimental conditions employed.
Keywords:
Antimicrobial activity Macrofungi Phallus indusiatus Whole Genome Sequencing (WGS)References
Amir, N., Kabir, T., Opsory, M. H., Nafisa, N. J. & Turna, N. I. (2025). Phytochemical profiling, antioxidant potential, and antimicrobial assessment of the essential oil extracted from Moringa oleifera. Undergraduate thesis. Department of Mathematics and Natural Sciences, BRAC University.
Aryanto Rahman, C., Santosa, D. & Kimia, K. (2022). Aktivitas rimpang temulawak sebagai antibakteri berdasarkan lokasi tumbuhnya: Narrative review. Jurnal Pharmascience, 9 (2).
Chen, S., Cao, L., Lv, T., Liu, J., Gao, G., Li, M., Sun, L., Tian, W., Tian, Y., Li, G. & Ma, T. (2025). Regulation mechanism of the long-chain n-alkane monooxygenase gene almA in Acinetobacter venetianus RAG-1. Applied and Environmental Microbiology, 91 (1). DOI: https://doi.org/10.1128/aem.02050-24
Frontini, M., Soutoglou, E., Argentini, M., Bole-Feysot, C., Jost, B., Scheer, E. & Tora, L. (2005). TAF9b (formerly TAF9L) is a bona fide TAF that has unique and overlapping roles with TAF9. Molecular and Cellular Biology, 25 (11): 4638–4649. DOI: https://doi.org/10.1128/MCB.25.11.4638-4649.2005
Gao, W., Zhang, L., Zhang, Y., et al. (2024). Genome-wide identification and expression analysis of the UBC gene family in wheat (Triticum aestivum L.). BMC Plant Biology, 24: 341. DOI: https://doi.org/10.1186/s12870-024-05042-3
Habtemariam, S. (2019). The chemistry, pharmacology and therapeutic potential of the edible mushroom Dictyophora indusiata (Vent ex. Pers.) Fischer (syn. Phallus indusiatus). Biomedicines, 7 (4): 98. DOI: https://doi.org/10.3390/biomedicines7040098
Hasugian, I. A. & Zikriyani, H. (2025). Literature review on the therapeutic potential of bacteriophages against resistant Staphylococcus aureus. Jurnal Biologi Tropis, 25 (4): 4869–4879. DOI: https://doi.org/10.29303/jbt.v25i4.9938
Kumar, Y. & Xu, B. (2025). New insights into chemical profiles and health-promoting effects of edible mushroom Dictyophora indusiata (Vent ex. Pers.) Fischer: A review. Journal of Fungi, 11 (1): 75. DOI: https://doi.org/10.3390/jof11010075
Lingga, R., Dalimunthe, N. P., Afriyansyah, B., Irwanto, R., Henri, H., Januardi, E., Marinah, M. & Safitri, S. (2021). Keanekaragaman jamur makroskopik di Hutan Wisata Desa Tiang Tarah Kabupaten Bangka. Bioma: Jurnal Ilmiah Biologi, 10 (2): 180–200. DOI: https://doi.org/10.26877/bioma.v10i2.7920
Ma, L. & Li, G. (2018). FAR1-RELATED SEQUENCE (FRS) and FRS-RELATED FACTOR (FRF) family proteins in Arabidopsis growth and development. Frontiers in Plant Science, 9: 692. DOI: https://doi.org/10.3389/fpls.2018.00692
Nurhayat, O. D., Putra, I. P., Anita, S. & Yanto, D. H. Y. (2021). Notes on some macro fungi from Taman Eden 100, Kawasan Toba, Sumatera Utara, Indonesia: Description and its potency. Bioeduscience, 5 (1): 30–39.
Pasqua, M., Bonaccorsi di Patti, M. C., Fanelli, G., Utsumi, R., Eguchi, Y., Trirocco, R., Prosseda, G., Grossi, M. & Colonna, B. (2021). Host-bacterial pathogen communication: The wily role of the multidrug efflux pumps of the MFS family. Frontiers in Molecular Biosciences, 8: 723274. DOI: https://doi.org/10.3389/fmolb.2021.723274
Prehatin, C., Butarbutar, M. E. T., Sianturi, S. & Fernandes, A. (2025). Formulasi dan evaluasi krim ekstrak jamur sawit (Volvariella sp.) sebagai tabir surya dengan variasi konsentrasi basis lemak tengkawang dan lemak cokelat. Majalah Farmasetika, 10 (1): 69–89. DOI: https://doi.org/10.24198/mfarmasetika.v10i1.60520
Risalba, I. (2024). KOBI successfully collected 11,137 data on Indonesian biodiversity. Faculty of Biology, Universitas Gadjah Mada.
Rödl, S., den Brave, F., Räschle, M., Kizmaz, B., Lenhard, S., Groh, C., Becker, H., Zimmermann, J., Morgan, B., Richling, E., Becker, T. & Herrmann, J. M. (2023). The metabolite-controlled ubiquitin conjugase Ubc8 promotes mitochondrial protein import. Life Science Alliance, 6 (1). DOI: https://doi.org/10.26508/lsa.202201526
Suryani, Y., Taupiqurrahman, O. & Kulsum, Y. (2020). Mikologi. PT. Freeline Cipta Granesia.
Venkateswara Prasad, U., Swarupa, V., Yeswanth, S., Santhosh Kumar, P., Siva Kumar, E., Mahesh Kumar Reddy, K., Nanda Kumar, Y., Jhansi Rani, V., Chaudhary, A. & Venkata Gurunadha Krishna Sarma, P. (2014). Structural and functional analysis of Staphylococcus aureus NADP-dependent IDH and its comparison with bacterial and human NADP-dependent IDH. Hypothesis, 10 (2).
White, C., Lee, J., Kambe, T., Fritsche, K. & Petris, M. J. (2009). A role for the ATP7A copper-transporting ATPase in macrophage bactericidal activity. Journal of Biological Chemistry, 284 (49): 33949–33956. DOI: https://doi.org/10.1074/jbc.M109.070201
Wolf, A. J., Reyes, C. N., Liang, W., Becker, C., Shimada, K., Wheeler, M. L., Cho, H. C., Popescu, N. I., Coggeshall, K. M., Arditi, M. & Underhill, D. M. (2016). Hexokinase is an innate immune receptor for the detection of bacterial peptidoglycan. Cell, 166 (3): 624–636. DOI: https://doi.org/10.1016/j.cell.2016.05.076
Yadav, P., Kim, N., Kumari, M., Verma, S., Sharma, T. K., Yadav, V. & Kumar, A. (2021). G-quadruplex structures in bacteria: Biological relevance and potential as an antimicrobial target. Journal of Bacteriology, 203 (13). DOI: https://doi.org/10.1128/JB.00577-20
Zhu, X., Guo, Y., Liu, Z., Yang, J., Tang, H. & Wang, Y. (2021). Itaconic acid exerts anti-inflammatory and antibacterial effects via promoting pentose phosphate pathway to produce ROS. Scientific Reports, 11 (1). DOI: https://doi.org/10.1038/s41598-021-97352-x
Zikriyani, H., Aryanto, A., Na’ilah, D. & Alifiyah, I. (2026). Tinjauan kuantitatif terhadap distribusi dan faktor lingkungan jamur Phallus sp. di Indonesia berdasarkan literatur. Jurnal Penelitian Sains, 28 (1D): 28. DOI: https://doi.org/10.56064/jps.v28i1.1201
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