Pathogenicity Characteristics of Front, Middle, and Hind Nipah Worm (Namalycastis rhodochorde) Digestive Tract Bacteria
DOI:
10.29303/jbt.v26i3.12427Published:
2026-08-02Downloads
Abstract
Nipah worms (Namalycastis rhodochorde) are one of Indonesia's invertebrate species used as high-protein aquaculture feed. The presence of pathogenic bacteria is a factor in the decline in quality and quantity in nipah worm cultivation, especially bacteria originating from the digestive tract of nipah worms. This study was conducted to examine the characteristics of pathogenicity of bacteria originating from the digestive tract of Nipah worms in the form of hemolysis and DNase activity. The hemolysis and DNase activity tests were performed using the streak plate method on Blood Agar Plate (BAP) and DNase Agar media. The results obtained in the hemolysis test showed a positive reaction in 68 isolates (90.7%), consisting of 5 α-hemolysis isolates, 63 β-hemolysis isolates, and 7 γ-hemolysis isolates. β hemolysis or complete hemolysis was found in 27 isolates in the foregut, 18 isolates in the midgut, and 18 isolates in the hindgut. The results obtained in the DNase activity test were positive in 17 isolates (22.7%), consisting of 9 isolates from the front digestive tract, 1 isolate from the middle digestive tract, and 7 isolates from the rear digestive tract. This study concludes that the high levels of hemolysis and DNase activity in bacterial isolates from the digestive tract of the nipah worm are predictors of pathogenicity. Bacterial isolates from the foregut showed the highest pathogenic activity, which is thought to be due to the presence of bacteria carried during the feeding process of the nipah worm.
Keywords:
Bacteria DNase Hemolysis Namalycastis rhodochorde Nipah wormReferences
Ayuningrum, D., Kristiana R., & Asagabaldan M. A. (2020). Potensi Bakteri Asosiasi Tunikata sebagai Penghasil Senyawa Antibakteri Guna Menghambat Pertumbuhan Bakteri Multidrug Resistant. Jurnal Pasir Laut, 4(2): 102-107. doi: 10.14710/jpl.2020.32807.
Edo, F. V., Junardi, & Yanti A. H. (2020). Kondisi Lingkungan dalam Pemeliharaan Larva Cacing Nipah Namalycastis rhodochorde di Laboratorium. Protobiont, 9(3): 219-223. doi: 10.26418/protobiont.v9i3.49208.
Hanif, M., Wicaksono R. R., Putri L. E., Misnarliah, & Risa R. D. B. (2026). Mikrobiologi Lingkungan: Studi tentang Mikroorganisme pada Ekosistem. Journal of Innovative and Creativity (Joecy), 6(1): 3020–3027. doi: 10.31004/joecy.v6i1.7234.
Harsa, I. M. S. (2025). Fisiologi Pencernaan dan Mikrobiota Usus. CV Jejak (Jejak Publisher). ISBN: 9786234989809
Horn, N., & Bhunia A. K. (2018). Food-associated Stress Primes Foodborne Pathogens for The Gastrointestinal Phase of Infection. Frontiers in microbiology, 9(1): 1962. doi: 10.3389/fmicb.2018.01962.
Istini, I. (2020). Pemanfaatan Plastik Polipropilen Standing Pouch sebagai Salah Satu Kemasan Sterilisasi Peralatan Laboratorium. Indonesian Journal of Laboratory, 2(3): 41-46. doi: 10.22146/ijl.v2i3.57424.
Junardi. (2018). Pengukuran Panjang Tubuh Cacing Nipah Pendek Namalycastis abiuma (Polychaeta: Neredidae) dari Perairan Mangrove Sungai Kapuas Kalimantan Barat. AL-Kauniyah Jurnal Biologi, 11(2): 183-189. doi: 10.15408/kauniyah.v11i2.7234.
Junardi & Riyandi. (2020). Sintasan dan Pertumbuhan Larva Cacing Nipah Namalycastis rhodochorde (Polychaeta: Nereididae) pada Budidaya dengan Dua Sumber Pakan Berbeda. Jurnal Akuakultur Rawa Indonesia, 8(2): 193-204. doi: 10.36706/jari.v8i2.11715
Karimela, E. J., Ijong F. G., & Dien H. A. (2017). Karakteristik Staphylococcus aureus yang Diisolasi dari Ikan Asap Pinekuhe Hasil Olahan Tradisional Kabupaten Sangihe. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(1): 188-198. doi: 10.17844/jphpi.v20i1.16506.
Koh, A., De Vadder F., Kovatcheva-Datchary P., & Bäckhed F. (2016). From Dietary Fiber to Host Physiology: Short-Chain Fatty Acids as Key Bacterial Metabolites. Cell, 165(6): 1332-1345. doi: 10.1016/j.cell.2016.05.041.
Lauková, L., Konečna B., Janovičová Ľ., Vlková B., & Celec P. (2020). Deoxyribonucleases and their applications in biomedicine. Biomolecules, 10(7): 1036. doi: 10.3390/biom10071036.
Novitriani, K., Sukmawan D. F., & Suhartati R. (2023). Isolasi dan Identifikasi Bakteri Patogen di Perairan Pantai Pangandaran. Prosiding Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia, 2 (1): 334-344. https://prosiding.aiptlmi-iasmlt.id/index.php/prosiding/article/view/229
Novriansyah, R., Suardana I. W., & Mahatmi H. (2025). Types of Hemolysis of Gram-Negative Bacteria Isolated from the Tonsils of Weaned Landrace Pigs. Buletin Veteriner Udayana, 17(2): 406-416. doi: 10.24843/bulvet.2025.v17.i02.p19.
Panirman, J. V., Setyawati T. R., & Junardi. (2023). Colonization of Nipa Worms (Namalycastis spp.) Based on Differences in Depth of Placement of Nipa Fronds in Kakap River of West Kalimantan. Jurnal Biologi Tropis, 23(2): 352-359. doi: 10.29303/jbt.v23i2.6165.
Pandiangan, P. P., Ester, Lumbantoruan L., Kaban H. B., Ndaha G. Y., & Pratiwi N. (2025). Studi Tinjauan Anatomi dan Fisiologi Sistem Pencernaan Manusia dalam Proses Penyerapan Nutrisi. Qistina, 4(2): 1311-1319. doi: 10.57235/qistina.v4i2.7279.
Pinatih, K. J. P., Sudarmana I. K., Aryanta W. R., & Widyastuti A. M. (2023). Phenotypic and Genotypic of Gram-Positive Coccus β-Hemolysis Bacteria Isolates from Tonsil and Nasal of Pig and Antimicrobial Susceptibility Test Against Penicillin G and Tetracycline. Bali Medical Journal, 12(3): 2407-2414. doi: 10.15562/bmj.v12i3.4372.
Rowland, I., Gibson G., Heinken A., Scott K., Swann J., Thiele I., & Tuohy K. (2017). Gut Microbiota Functions: Metabolism of Nutrients and Other Food Components. European journal of nutrition, 57(1): 1-24. doi: 10.1007/s00394-017-1445-8.
Salsabila, T. S., Setyawati T. R., & Junardi. (2024). Sintasan dan Pertumbuhan Larva Cacing Nipah (Namalycastis rhodochorde) yang Diberi Pakan Chlorella vulgaris dengan Konsentrasi Berbeda. Berita Biologi, 23(3): 455-465. doi: 10.55981/berita_biologi.2024.5015.
Sanatang & Lio T. M. P. (2021). Bacterial Screening on Banana Skin by Using Nutrient Agar and Blood Agar Media. Bioma. 6(1): 31-37. doi: 10.20956/bioma.v6i1.11822.
Sodiq, A. H., Setiawati M. R., Santosa D. A., & Widayat D. (2019). Potensi Mikroba Asal Mikroorganisme Lokal dalam Meningkatkan Perkecambahan Benih Paprika. Jurnal Agroekoteknologi, 11(2): 214-226. doi: 10.33512/jur.agroekotek.v11i2.7694.
Sukmadewi, D. K. T., Anas I., Widyastuti R., & Cintaresmini A. (2017). Uji Fitopatogenitas, Hemolisis serta Kemampuan Mikrob dalam Melarutkan Fosfat dan Kalium. Jurnal Ilmu Tanah dan Lingkungan, 19(2): 68-73. doi: 10.29244/jitl.19.2.68-73.
Suryani, E. M., & Gaffar A. (2024). Isolasi dan karakterisasi bakteri asam laktat dari susu kuda Bima (Equus sp.) yang berpotensi sebagai probiotik. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 9(2), 102-108. doi: 10.33474/e-jbst.v9i2.561.
Sutandi, M. S. A., Budiarti S., & Priyanto J. A. (2024). Isolasi, Karakterisasi, dan Identifikasi Bakteri Penghasil Hemolisin dari Penderita Gingivitis. Jurnal Sumberdaya Hayati, 10(4): 162-169. doi: 10.29244/jsdh.10.4.162-169.
Windria, S., Cahyaningtyas A. A., Cahyadi A. I., Wiraswati H. L., & Ramadhanti J. (2023). Identifikasi Fenotip dan Genotip Staphylococcus aureus Isolat Asal Susu Sapi Perah Mastitis Subklinis di Wilayah Pamulihan, Kabupaten Sumedang Jawa Barat. Jurnal Sain Veteriner, 41(2): 215-225. doi: 10.22146/jsv.76052.
Yanti, A. H., Setyawati T. R., & Kurniatuhadi R. (2019). Karakterisasi Kapang dari Saluran Pencernaan Cacing Nipah (Namalycastis rhodochorde) Asal Desa Sungai Kakap, Kabupaten Kubu Raya, Kalimantan Barat. Life Science, 8(2): 113-125. doi: 10.15294/lifesci.v8i2.37098.
License
Copyright (c) 2026 Dian Indrawati, Ari Hepi Yanti, Rikhsan Kurniatuhadi
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
