Effects of Salinity and Concentration PGPR Bidara Root ACC Deaminase- on Chili Growth and Yield
DOI:
10.29303/jbt.v26i3.12627Published:
2026-07-11Downloads
Abstract
Soil salinity is an escalating challenge in dryland agriculture, where rising electrical conductivity disrupts water balance and nutrient uptake. These stresses stimulate excessive ethylene production, which suppresses root growth and reduces overall plant vigor. Plant growth–promoting rhizobacteria (PGPR) with ACC deaminase activity offer a promising mitigation strategy by degrading ACC, the ethylene precursor, thereby helping plants maintain growth under saline conditions. This study evaluated the effect of different concentrations of ACC deaminase producing bacteria isolated from Bidara (Ziziphus mauritiana Lam.) roots on chili (Capsicum annum L.) growth under graded salinity stress. Bacterial cultures were harvested at OD₆₀₀ = 0.8 and applied at three concentrations (0, 10, and 20 mL/L) to chili seedlings exposed to four salinity levels (0, 2, 4, and 8 dS/m). Growth parameters—including plant height, leaf number, biomass, and root development—were measured to assess physiological responses. Results showed that increasing salinity progressively reduced plant growth, with substantial inhibition observed at 8 dS/m. In contrast, bacterial application significantly improved plant performance across all salinity levels, and the 20 mL/L concentration consistently produced the greatest enhancement. The improved growth is attributed to enhanced root system function and chlorophyl concentration. These findings highlight the essential role of ACC deaminase producing bacteria in mitigating salinity stress in chili cultivation under dryland conditions and underscore the importance of optimizing bacterial concentration to maximize their beneficial effects.
Keywords:
ACC deaminase Capsicum annuum Dryland agriculture Plant growth-promoting rhizobacteria (PGPR) Salinity stress Ziziphus mauritianaReferences
Aisy, S. P., & Rachmawati, D. (2022). Respons Pertumbuhan Tanaman Cabai Rawit (Capsicum frutescens L.) terhadap Perlakuan Priming PEG dalam Mengatasi Cekaman Salinitas. Bioscientist: Jurnal Ilmiah Biologi, 10(2), 868-880. https://doi.org/10.33394/bioscientist.v10i2.6122
Alonazi, M. A., Alwathnani, H. A., AL-Barakah, F. N. I., & Alotaibi, F. (2025). Native Plant Growth-Promoting Rhizobacteria Containing ACC Deaminase Promote Plant Growth and Alleviate Salinity and Heat Stress in Maize (Zea maysL.). Journal Plants,14(7),1–20. https://doi.org/10.3390/plants14071107
Badan Pusat Statistik. (2023). Statistik Hortikultura 2023. Jakarta: Badan Pusat Statistik.
Candraningtyas, C. F., & Indrawan, M. (2023). Analisis efektivitas penggunaan Plant Growth Promoting Rhizobacteria (PGPR) untuk peningkatan pertanian berkelanjutan. Risalah Kebijakan Pertanian Dan Lingkungan Rumusan Kajian Strategis Bidang Pertanian dan Lingkungan, 10(2), 88-99.
Dawan, M., Ogie, T. B., & Kaligis, J. B. (2024). The Effect Of Giving Plant Growth Promoting Rhizobacteria (PGPR) On The Growth Of Mustard Plants (Brassica juncea L.). Jurnal Agroekoteknologi Terapan, 5(1), 13-19.
Gupta, A., Rai, S., Bano, A., Sharma, S., Kumar, M., Binsuwaidan, R. (2022). ACC Deaminase Produced by PGPR Mitigates the Adverse Effect of Osmotic and Salinity Stresses in Pisum sativum through Modulating the Antioxidants Activities. Plants. https://doi.org/10.3390/plants11243419
Husein, E., Saraswati, R., & Husen, E. (2015). Mikroorganisme tanah yang bermanfaat pada tanaman. Bogor: Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian.
Jannah, M., Jannah, R., & Fahrunsyah. (2020). Penggunaan Plant Growth Promoting Rhizobacteria (PGPR) untuk Meningkatkan Pertumbuhan dan Mengurangi Pemakaian Pupuk Anorganik pada Tanaman Pertanian. Jurnal Agroekoteknologi Tropika Lembab, 5(1), 41–49. https://e-journals.unmul.ac.id/index.php/agro/article/view/7940
Jannah, M., Surtiningsih, T., & Nurhariyati, T. (2020). Potensi Plant Growth Promoting Rhizobacteria (PGPR) dalam meningkatkan pertumbuhan tanaman. Jurnal Biologi Tropis, 20(2), 272–281.
Karolinoerita, V., & Annisa, W. (2020). Salinisasi lahan dan permasalahannya di Indonesia. Jurnal Sumberdaya Lahan, 14(2), 91-99. https://doi.org/10.21082/jsdl.v14n2.2020.91-99
Karolinoerita, V., & Annisa, W. (2020). Salinitas lahan dan strategi pengelolaannya di Indonesia. Bogor: Balai Penelitian Tanah, Kementerian Pertanian.
Kuswandi, N. K. &. (2021). Pengaruh Pemberian Berbagai Jenis Pupuk Dasar Terhadap Pertumbuhan dan Hasil Cabai (Capsicum annum L) . Jurnal Pembangunan Nagari. 6(2), 203–212. https://doi.org/10.30559/jpn.v
Mardhiana, F., Soeparjono, S., & Handoyo, T. (2018). Pengaruh Konsentrasi dan Waktu Aplikasi NaCl terhadap Hasil dan Mutu Cabai Merah (Capsicum annum L .). 2(1), 1–8. https://doi.org/10.25047/agriprima.v2i1.81
Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651–681. https://doi.org/10.1146/annurev.arplant.59.032607.092911
Nikmatullah A, Sarjan M, Dewi S.m., Dijkwel P, Haryanto H, 2025. Bioprospecting Indigenous Microorganisms from Semi-Arid Soil as Biostimulant and Biocontrol Agents towards Sustainable Potato Production under Climate Change. Laporan Penelitian Kerjasama Luar Negeri Dana PNBP Universitas Mataram, Universitas Mataram, Mataram.
Nurhaliza, F., Aminah, A., & Saida, S. (2025). RESPON TANAMAN CABAI RAWIT (Capsicum frutescens L.) VARIETAS DEWATA PADA KONDISI CEKAMAN SALINITAS. AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian, 6(1), 101-109.
Puspitasari, I.D., Muslihatin, W., dan Agisimanto, D. (2017). Pertumbuhan Kalus Jeruk JC (Japansche Citroen) pada Media Murashige and Skoog dengan Berbagai Konsentrasi NaCl. Jurnal Sains dan Seni ITS, 6(2), 2337-3520.
Santoyo, G., Flores, C. A., Lara, P. D. L., Mosqueda, M. C. O., Glick, B. R. (2021). Rhizosphere Colonization Determinants by Plant Growth-Promoting Rhizobacteria (PGPR). Biology. 27(5). 1–18. https://doi.org/10.3390/biology 10060475
Shahid, M., Khan, M. S., & colleagues. (2023). ACC deaminase-producing plant growth-promoting rhizobacteria improve plant tolerance to salinity stress. Frontiers in Plant Science, 14, 1178456. https://doi.org/10.3389/fpls.2023.1178456
Shahid, M., Singh, U. B., Khan, M. S., Singh, P., Kumar, R., Singh, R. N., Kumar, A., & Singh, H. V. (2023). Bacterial ACC deaminase: Insights into enzymology, biochemistry, genetics, and potential role in amelioration of environmental stress in crop plants. Frontiers in Microbiology. 14(April). https://doi.org/10.3389/fmicb.2023.1132770
Singh, R. P. (2022). Plant growth-promoting rhizobacteria for sustainable agriculture: Recent developments and future prospects. Frontiers in Microbiology, 13, 1014444. https://doi.org/10.3389/fmicb.2022.1014444
Singh, S. K. (2022). Effect of Plant Growth Promoting Rhizobacteria on Potato. Potato Journal. 33(1), 164–165. https://doi.org/10.20956/ecosolum.V11i1.21144
Suryaman, M., Kurniati, F., & Khaerunisa, H. (2021). Pertumbuhan Kedelai pada Kondisi Cekaman Salinitas dengan Pemberian Ekstrak Kulit Buah Nanas ( Ananas comosus L ). Jurnal Penelitian Pertanian Terapan. 22(2), 186–194. https://www.academia.edu/download/107152905/1581.pdf
Syamsiah, M., & Rayani, W. (2014). Pengaruh aplikasi PGPR terhadap pertumbuhan dan hasil tanaman. Jurnal Agroteknologi, 8(2), 85–92.
Ulum, B., Rachmawati, D., & Saptadi, D. (2023). Physiological responses of plants under salinity stress: A review. IOP Conference Series: Earth and Environmental Science, 1225, 012034.
Yusuf, M., Rupang, M. S., Sembiring, J., Anwar, A., Ekowati, N. Y., Mekiuw, Y., & Malesi, W. O. A. W. (2026). Artikel Reviu: Dampak Salinitas pada Kesuburan Tanah serta Respons Fisiologi Tanaman. Agroteknika, 9(1), 85-99.
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