Antibacterial Activity Test of 70% Ethanol Extract of Kersen Leaves (Muntingia calabura L.) against Cutibacterium acnes
DOI:
10.29303/jbt.v26i3.12838Published:
2026-07-25Downloads
Abstract
Acne ranks among the most prevalent skin issues and is linked to infection by Cutibacterium acnes. The rising rates of antibiotic resistance seen in Cutibacterium acnes necessitate the exploration of alternative plant-based treatments with antibacterial properties. One such plant that has demonstrated these antibacterial effects is the kersen leaf (Muntingia calabura L.). This research aimed to evaluate the antibacterial effects of a 70% ethanol extract of kersen leaves (Muntingia calabura L.) on the proliferation of Cutibacterium acnes. The research employed a true experimental design featuring a posttest only control group. The treatment categories included a positive control using Clindamycin, a negative control with sterile aquadest, and varying concentrations of kersen leaf extract at 1%, 2%, 4%, 8%, and 12%, with each group replicated four times. Data were assessed using the One-Way ANOVA method. The findings of the antibacterial test indicated that the 70% ethanol extract of kersen leaves effectively inhibited the growth of Cutibacterium acnes. The One-Way ANOVA analysis produced a p-value of less than 0.0001, which is lower than α = 0.05, signifying a notable discrepancy in the average inhibition zone diameters across the treatment groups. Ethanol extract from kersen leaves (Muntingia calabura L.) exhibits antibacterial properties against Cutibacterium acnes growth.
Keywords:
Antibacterial Cutibacterium acnes Muntingia calabura L.References
Adelila., Sari., Ernita., Mellya., Mara, M., & Rudi, M. (2020). Identification of active compounds on Muntingia calabura L. leaves using different polarity solvents. Indonesian Journal of Chemical Science and Technology, 3, 1–7. 10.24114/ijcst.v3i1.18309
Afifah, N., Riyanta, A. B., & Amananti, W. (2023). Pengaruh waktu maserasi terhadap hasil skrining fitokimia pada ekstrak daun mangga harum manis (Mangifera indica L.). Journal of Crystal: Publikasi Penelitian Kimia Terapan, 5(1), 54–61. https://doi.org/10.36526/jc.v5i1.2634
Agustina, L., Lailiyah, M., Yuliati, N., Prasongko, E. T., Istiqomah, N., Soehartono, D. R., & Dayoob, M. (2025). Formulation and evaluation of antibacterial activity of emulgel preparation containing Muntingia calabura L. leaves extract. Universal Journal of Pharmaceutical Research, 10(2). https://doi.org/10.22270/ujpr.v10i2
Bamasri, T. H. (2021). Daun kersen (Muntingia calabura) sebagai antibakteri. Jurnal Penelitian Perawat Profesional, 3(2), 351–358. https://doi.org/10.37287/jppp.v3i2.396
Byrd, A. L., Belkaid, Y., & Segre, J. A. (2018). The human skin microbiome. Nature Reviews Microbiology, 16(3), 143–155. https://doi.org/10.1038/nrmicro.2017.157
Desrini, S., & Ghiffary, H. M. (2018). Comparison of antibacterial activity of talok (Muntingia calabura L.) leaves ethanolic and n-hexane extracts on Propionibacterium acnes. AIP Conference Proceedings, 1954, 030002. https://doi.org/10.1063/1.5030328
Dessinioti, C., & Katsambas, A. (2024). The microbiome and acne: Perspectives for treatment. Dermatology and Therapy, 14(1), 31–44. https://doi.org/10.1007/s13555-023-01068-6
Dzotam, J. K., Kuete, V., & Efferth, T. (2024). Natural products and their antibacterial mechanisms against multidrug-resistant bacteria: An updated review. Pharmaceuticals, 17(2), 214. https://doi.org/10.3390/ph17020214
Estevania, N., Purbosari, I., & Mukti, A. W. (2024). Perbandingan aktivitas antibakteri ekstrak etanol 70% dan 96% daun kumis kucing (Orthosiphon aristatus) terhadap bakteri Escherichia coli. Jurnal Kesehatan Tambusai, 5(3), 32098–32106.
Farhadi, F., Khameneh, B., Iranshahi, M., & Iranshahy, M. (2025). Plant polyphenols as antibacterial agents: Mechanisms of action and therapeutic prospects. Frontiers in Microbiology, 16, 1502387. https://doi.org/10.3389/fmicb.2025.1502387
Galinier, A., Delan-Forino, C., Foulquier, E., Lakhal, H., & Pompeo, F. (2023). Recent advances in peptidoglycan synthesis and regulation in bacteria. Biomolecules, 13(5), 720. https://doi.org/10.3390/biom13050720
Handayani, V. (2015). Pengujian aktivitas antibakteri ekstrak etanol daun kersen (Muntingia calabura L.) terhadap bakteri penyebab jerawat. Jurnal Fitofarmaka Indonesia, 2(1), 94–99. https://doi.org/10.33096/jffi.v2i1.186
Jawetz, E., Melnick, J. L., & Adelberg, E. A. (2012). Mikrobiologi kedokteran (25th ed.). EGC.
Lupitasari, H., & Azzahra, F. (2025). Perbandingan konsentrasi pelarut terhadap rendemen dan hasil skrining fitokimia ekstrak daun kersen (Muntingia calabura). Jurnal Kefarmasian Akfarindo, 35–43. https://doi.org/10.37089/jofar.vi0.1110
Madelina, W., & Sulistiyaningsih. (2018). Review: Resistensi antibiotik pada terapi pengobatan jerawat. Farmaka, 16(2), 105–117. https://doi.org/10.24198/jf.v16i2.17665
Majoumouo, M. S., Tamokou, J. D. D., & Kuete, V. (2023). Mechanisms of antibacterial action of plant secondary metabolites: A comprehensive review. Biomedicine & Pharmacotherapy, 166, 115425. https://doi.org/10.1016/j.biopha.2023.115425
McLaughlin, J., Watterson, S., Layton, A. M., Bjourson, A. J., Barnard, E., & McDowell, A. (2019). Propionibacterium acnes and acne vulgaris: New insights from the integration of population genetic, multi-omic, biochemical and host–microbe studies. Microorganisms, 7(5), 128. https://doi.org/10.3390/microorganisms7050128
Milanda, T., Chandra, R. A. I., & Dwipratama, A. J. (2021). Formulasi dan pengujian aktivitas antibakteri krim ekstrak etanol daun kapuk (Ceiba pentandra L.). Majalah Farmasetika, 6(2), 138–149. https://doi.org/10.24198/mfarmasetika.v6i2
Mustarichie, R., Sulistyaningsih, S., & Runadi, D. (2020). Antibacterial activity test of extracts and fractions of cassava leaves (Manihot esculenta Crantz) against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes causing acne. International Journal of Microbiology, 2020, Article 8831800, 1–9. https://doi.org/10.1155/2020/8831800
Mustarichie, R., Sulistyaningsih, S., & Runadi, D. (2020). Antibacterial activity test of extracts and fractions of cassava leaves (Manihot esculenta Crantz) against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes causing acne. International Journal of Microbiology, 2020, Article 8831800, 1–9. https://doi.org/10.1155/2020/8831800
O'Neill, A. M., Nakatsuji, T., & Gallo, R. L. (2023). Host–microbiome interactions and antimicrobial resistance in acne vulgaris. Journal of Investigative Dermatology, 143(6), 1115–1123. https://doi.org/10.1016/j.jid.2023.01.019
Yustian, A. A., Darsono, P. V., Hakim, A. R., & Mahdiyah, D. (2025). Aktivitas antibakteri ekstrak daun mahang (Macaranga triloba (Thunb.) Mull. Arg.) terhadap Propionibacterium acnes. Sains Medisina, 3(3), 104–111. https://doi.org/10.63004/snsmed.v3i3.617
Zaenglein, A. L., Pathy, A. L., Schlosser, B. J., Alikhan, A., Baldwin, H. E., Berson, D. S., ... & Bhushan, R. (2016). Guidelines of care for the management of acne vulgaris. Journal of the American academy of dermatology, 74(5), 945-973. https://doi.org/10.1016/j.jaad.2023.12.019
Zahrah, H., Mustika, A., & Debora, K. (2019). Aktivitas antibakteri dan perubahan morfologi dari Propionibacterium acnes setelah pemberian ekstrak Curcuma xanthorrhiza. Jurnal Biosains Pascasarjana, 20(3), 160–169. https://doi.org/10.20473/jbp.v20i3.2018.160-169
Zhang, L., Ravipati, A. S., Koyyalamudi, S. R., Jeong, S. C., Reddy, N., Bartlett, J., Smith, P. T., de la Cruz, J., Monteiro, M. C., & Wu, M. J. (2021). Research progress on antibacterial activities and mechanisms of natural alkaloids: A review. Antibiotics, 10(3), 318. https://doi.org/10.3390/antibiotics10030318
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