Vol. 26 No. 3 (2026): in Progress
Open Access
Peer Reviewed

Antibacterial Activity Assay of 96% Ethanol Extract of Strawberry Leaves (Fragaria × ananassa Duch.) Against Streptococcus mutans Using the Disc Diffusion Method and Phytochemical Analysis

Authors

Mahfur Mahfur , Priatin Nova Adelina

DOI:

10.29303/jbt.v26i3.12903

Published:

2026-08-07

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Abstract

Abesah, N. H. S., & Mahfur, M. (2024). Isolasi dan identifikasi antioksidan jamur endofit umbi talas (Colocasia esculenta (L.) Schott) menggunakan pereaksi semprot DPPH secara bioautografi. Jurnal Insan Farmasi Indonesia, 7(3), 367–378. https://doi.org/10.36387/jifi.v7i3.2235

Allo. (2016). Uji aktivitas antibakteri dari ekstrak air kulit buah pisang Ambon lumut (Musa acuminata Colla) terhadap pertumbuhan Staphylococcus aureus (Skripsi). Universitas Sanata Dharma.

Andesmora, E. V., Muhadiono, M., & Hilwan, I. (2017). Ethnobotanical study of plants used by people in Hiang Indigenous Forest, Kerinci, Jambi. Journal of Tropical Life Science, 7(2), 95–101.

Bakti, T. E. K., & Mahfur, M. (2023). Skrining fitokimia dan analisis kadar flavonoid total fraksi etil asetat kulit buah durian merah (Durio graveolens Becc.) dengan metode spektrofotometri UV–Vis. Journal of Pharmacy Science and Technology, 4(1), 30–35. https://doi.org/10.30649/pst.v4i1.57

Bouyahya, A., et al. (2016). Determination of phenolic contents, antioxidant and antibacterial activities of strawberry tree (Arbutus unedo L.) leaf extracts. British Biotechnology Journal, 14(3), 1–10. https://doi.org/10.9734/BBJ/2016/26488

Elfeky, N., Mohamed Mousa, A. M., Abdelsameea Mahmoud, E., Mohammed Awad, S., El-Sabbagh, S. M., & Zayed, M. (2023). Antimicrobial activities of Fragaria × ananassa leaves against multidrug-resistant Acinetobacter baumannii. Delta Journal of Science, 47(1), 154–170. https://doi.org/10.21608/djs.2023.231555.1127

Engleberg, N. C., DiRita, V., & Dermody, T. S. (2006). Schaechter's mechanisms of microbial disease (4th ed.). Lippincott Williams & Wilkins.

Fajrina, A., Jubahar, J., & Hardiana, N. (2017). Uji aktivitas fraksi dari ekstrak akar kangkung (Ipomoea aquatica Forssk.) terhadap bakteri Streptococcus mutans. Jurnal Farmasi Higea, 9(2). (DOI yang dicantumkan perlu diverifikasi karena tidak sesuai dengan jurnal).

Ginting, M., Ginting, P., & Sari, S. A. (2023). Studi aktivitas antibakteri ekstrak etanol daun stroberi (Fragaria × ananassa (Weston) Rozier) terhadap bakteri Propionibacterium acnes. Journal of Pharmaceutical and Sciences, 6(1), 281–286. https://doi.org/10.36490/journal-jps.com.v6i1.56

He, M., Huang, Y., Wang, J., Chen, Z., Xie, J., Cui, Z., et al. (2025). Advances in polysaccharide-based antibacterial materials. International Journal of Biological Macromolecules, 308, 142598. (DOI perlu diverifikasi karena DOI yang dicantumkan mengarah ke jurnal lain.)

Hirsch, M., Burges, P. L., Migueliz, L., Villarreal, N. M., & Marina, M. (2024). Isolation of beneficial bacteria from strawberry (Fragaria × ananassa Duch.): Potentialities for fungal disease control and plant growth promotion. Plant Growth Regulation, 102(1), 135–152.

Jannah, A., Rachmawaty, D. U., & Maunatin, A. (2017). Uji aktivitas antibakteri ekstrak etanol, etil asetat, dan petroleum eter rambut jagung manis (Zea mays saccharata Sturt) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Alchemy: Journal of Chemistry, 5(4).

Kaur, A., Goindi, S., & Katare, O. P. (2016). Thermal analysis and quantitative characterization of compatibility between diflunisal and lipid excipients as raw materials for development of solid lipid nanoparticles. Thermochimica Acta, 643, 23–32.

Mahfur, M. (2016). Uji sitotoksik fraksi etil asetat ekstrak etanol akar pasak bumi (Eurycoma longifolia Jack) terhadap sel kanker T47D dengan metode MTT. Pena: Jurnal Ilmu Pengetahuan dan Teknologi, 30(2), 57–64. https://doi.org/10.31941/jurnalpena.v30i2.497

Mahfur, M., Khasanah, K., Anindhita, M. A., Chandra, S. G., & Hidayah, A. N. (2023). Comparison of the total phenolic and flavonoid contents of Amomum compactum Sol. ex Maton from Linggo Asri and Paninggaran Districts, Pekalongan Regency. Sasambo Journal of Pharmacy, 4(1), 8–13. https://doi.org/10.29303/sjp.v4i1.212

Mahfur, M., Kamila, A., Abidah, J. F., Samirana, P. O., Hikmawan, B. D., & Pratimasari, D. (2023). Antibacterial activity of ethyl acetate extract of symbiont fungus Aspergillus sp. in the sponge Rhabdastrella sp. from Gili Layar Island, Lombok, Indonesia. Journal of Applied Pharmaceutical Science, 13(11), 144–151. https://doi.org/10.7324/japs.2023.146303

Mahnashi, M. H., & Alshehri, O. M. (2022). Isolation, in vitro and in silico anti-Alzheimer and anti-inflammatory studies on phytosteroids from aerial parts of Fragaria × ananassa Duch. Biomolecules, 12(10), 1430.

Mazurel, D., Brandt, B. W., Boomsma, M., Crielaard, W., Lagerweij, M., Exterkate, R. A. M., & Deng, D. M. (2025). Streptococcus mutans and caries: A systematic review and meta-analysis. Journal of Dental Research, 104(6), 594–603.

Rani, K., & Mahfur, M. (2023). Analisis kadar flavonoid total ekstrak bonggol pisang kepok kuning (Musa acuminata Colla) secara spektrofotometri UV–Vis. Benzena Pharmaceutical Scientific Journal, 2(1). (Halaman perlu dilengkapi.)

Rusmalina, S., Mahfur, M., Ermawati, N., Maliah, N., Ananda, L., Pratama, K. B., et al. (2025). The optimization of fermentation for metabolite production by symbiont Penicillium nalgiovense from the sponge Gelliodes fibulata. Medical Sains: Jurnal Ilmiah Kefarmasian, 10(1), 9–18. https://doi.org/10.37874/ms.v10i1.1682

Salas-Arias, K., Irías-Mata, A., Sánchez-Kopper, A., Hernández-Moncada, R., Salas-Morgan, B., Villalta-Romero, F., & Calvo-Castro, L. A. (2023). Strawberry (Fragaria × ananassa cv. Festival): A polyphenol-based phytochemical characterization in fruit and leaf extracts. Molecules, 28(4), 1865. https://doi.org/10.3390/molecules28041865

Widyaningtias, et al. (2014). Uji aktivitas antibakteri ekstrak terpurifikasi daun sirih hijau (Piper betle L.) terhadap bakteri Propionibacterium acnes. Jurnal Farmasi Udayana, 3(1), 50–57. (Volume, nomor, dan halaman perlu diverifikasi.)

Widiatmoko, H. (2016). Sehat tanpa obat dengan si merah stroberi. Rapha Publishing.

Ye, D., Liu, Y., Li, J., Zhou, J., Cao, J., Wu, Y., et al. (2025). Competitive dynamics and balance between Streptococcus mutans and commensal streptococci in oral microecology. Critical Reviews in Microbiology, 51(3), 532–543.

Zhou, Y., Chen, X., Chen, T., & Chen, X. (2022). A review of the antibacterial activity and mechanisms of plant polysaccharides. Trends in Food Science & Technology, 123, 264–280. https://doi.org/10.1016/j.tifs.2022.03.020

Keywords:

Antibacteria Fragaria x ananassa Duch Phytochemica Streptococcus mutans

References

Andesmora, E. V, Muhadiono, M., & Hilwan, I. (2017). Ethnobotanical Study of Plants Used by People in Hiang Indigenous Forest Kerinci Jambi. Journal of Tropical Life Science, 7(2), 95–101.

Allo. (2016). Uji Aktivitas Antibakteri dari Ekstrak Air Kulit Buah Pisang Ambon Lumut (Musa acuminata Colla) terhadap Pertumbuhan Staphylococcus aureus. [skripsi] Universitas Sanata Dharma.

Bouyahya, A., & dkk. (2016). Determination Of Phenolic Contents, Antioxidant and Antibacterial Activities of Strawberry Tree (Arbutus Unedo L.) Leaf Extracts. Br Biotechnol J, 14(3), 1–10.

Engleberg, N. C., V. Dirita, and T. S. dermody. (2006). Schaechter’s Mechanisms of Microbial Disease. Edisi 4. USA: Lippincott Williams & Wilkins.

Fajrina, A., Jubahar, J., & Hardiana, N. (2017). Uji Aktivitas Fraksi Dari Ekstrak Akar Kangkung (Ipomoea Aquatica Forssk.) Terhadap Bakteri Streptococcus Mutans. Jurnal Farmasi Higea, 9(2).

Ginting Mandike, & dkk. (2023). Study of Antibacterial Activity of Strawberry Leaf Ethanol Extract (‭Fragaria x ‬ananassa ‭(Weston) Rozier) Against ‬Propionibacterium acnes‭ Bacteria. Medan: JPS, 6(1), 281–286.‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬

Jannah, A., Rachmawaty, D. U., & Maunatin, A. (2017). Uji Aktivitas Antibakteri Ekstrak Etanol, Etil Asetat Dan Petroleum Eter Rambut Jagung Manis (Zea Mays Ssaccarata Strurt) Terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli. J. In Alchemy, 5(4).

Kaur, A., Goindi, S., & Katare, O. P. (2016). Thermal Analysis and Quantitative Characterization of Compatibility Between Diflunisal and Lipid Excipients as Raw Materials for Development of Solid Lipid Nanoparticles. Thermochimica Acta, 643, 23–32.

Leboffe, M. J., & Pierce, B. E. (2011). A Photographic Atlas for The Microbiology Laboratory. Colorado: Morton Publishing Company.

Salas-Arias, K., Irías-Mata, A., Sánchez-Kopper, A., Hernández-Moncada, R., Salas-Morgan, B., Villalta-Romero, F., & Calvo-Castro, L. A. (2023). Strawberry (Fragaria × ananassa cv. Festival): A Polyphenol-Based Phytochemical Characterization in Fruit and Leaf Extracts. Molecules, 28(4), 1865. https://doi.org/10.3390/molecules28041865

Widyaningtias, dkk. (2014). Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Sirih Hijau (Piper Betle L.) terhadap Bakteri Propionibacterium Acnes. J Farm Udayana, 50(3).

Widiatmoko, H. (2016). Sehat Tanpa Obat dengan Si Merah Stroberi. Rapha Publishing.

Author Biographies

Mahfur Mahfur, Universitas Pekalongan

Author Origin : Indonesia

Priatin Nova Adelina, Universitas Pekalongan

Author Origin : Indonesia

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How to Cite

Mahfur, M., & Adelina, P. N. (2026). Antibacterial Activity Assay of 96% Ethanol Extract of Strawberry Leaves (Fragaria × ananassa Duch.) Against Streptococcus mutans Using the Disc Diffusion Method and Phytochemical Analysis. Jurnal Biologi Tropis, 26(3), 1262–1268. https://doi.org/10.29303/jbt.v26i3.12903

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