Evaluation of Entomopathogenic Bacteria Isolated from Bamboo Rhizosphere Soil in Mataram City and Their Toxicity Against Instar III Aedes aegypti Larvae in a Laboratory Environment
DOI:
10.29303/jbt.v26i4.13026Published:
2026-10-09Downloads
Abstract
Aedes aegypti is an important vector of dengue and other mosquito-borne diseases. Entomopathogenic bacteria from the bamboo rhizosphere may provide a potential biological approach for controlling its larvae. This study aimed to isolate entomopathogenic bacteria that are able to suppress Aedes aegypti mosquito larvae in a laboratory environment. Bamboo rhizosphere soil samples were collected at several selected bamboo rhizosphere locations around Mataram City. Two hundred and fifty grams of soil was collected from a depth of 5-10 cm below the surface and placed into pre-sterilized containers. The soil in the containers was stored in a shaded area before use in the study. The bacteria were isolated by heat treatment the soil mixed with sterile distilled water (10% concentration), then conducted to serial dilution and spreading it on Nutrient Agar media supplemented with Amoxicillin and Streptomycin. The growing colonies of bacteria were then tested for their toxicity on Instar III Aedes aegypti larvae in the laboratory to determine their toxicity. Bacterial isolation from all bamboo rhizosphere locations yielded two entomopathogenic bacterial species, Bacillus thuringiensis and Bacillus sphaericus. B. thuringensis isolated from several locations exhibited toxicity to instar III A. aegypti larvae ranging from 30% to 60%, while B. sphaericus exhibited toxicity ranging from 10% to 30%. The toxicity values demonstrated by these two bacteria were categorized as low to moderate. Differences in toxicity levels between isolates confirm the influence of habitat on the bacteria's biological characteristics. This finding reinforces the role of indigenous microorganisms as an environmentally friendly and sustainable source of vector control.
Keywords:
Aedes aegypti larvae Bacillus sphaericus Bacillus thuringiensis Bamboo rhizosphere Entomopathogenic bacteriaReferences
Abbott, W. S. (1925). A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology, 18(2), 265–267. https://doi.org/10.1093/jee/18.2.265a
Adang, M. J., Crickmore, N., & Jurat-Fuentes, J. L. (2014). Diversity of Bacillus thuringiensis Crystal Toxins and Mechanism of Action. In Advances in Insect Physiology (Vol. 47, pp. 39–87). Elsevier. https://doi.org/10.1016/B978-0-12-800197-4.00002-6
Alramadan, Y. & Mamay, M. (2019). The Importance of Entomopathogenic Fungi in The Control of Agricultural Pests and Promising Fungal Entomopathogens in The Field Application. 1st International Gobleklitepe Internasional Congress.
Aswathi, N., Balakrishnan, N., Srinivasan, T., Kokiladevi, E., & Raghu, R. (2024). Diversity of Bt toxins and their utility in pest management. Egyptian Journal of Biological Pest Control, 34, 40. https://doi.org/10.1186/s41938-024-00803-6
Bay, S. K., McGeoch, M. A., Gillor, O., Wieler, N., Palmer, D. J., Baker, D. J., Chown, S. L., & Greening, C. (2020). Soil bacterial communities exhibit strong biogeographic patterns at fine taxonomic resolution. mSystems, 5(4), e00540-20. https://doi.org/10.1128/mSystems.00540-20
Belousova, M. E., Malovichko, Y. V., Shikov, A. E., Nizhnikov, A. A., & Antonets, K. S. (2021). Dissecting the Environmental Consequences of Bacillus thuringiensis Application for Natural Ecosystems. Toxins, 13(5), 355. https://doi.org/10.3390/toxins13050355
Carson, J., Erriah, B., Herodotou, S., Shtukenberg, A. G., Smith, L., Ryazanskaya, S., Ward, M. D., Kahr, B., & Lees, R. S. (2023). Overcoming insecticide resistance in Anopheles mosquitoes by using faster-acting solid forms of deltamethrin. Malaria Journal, 22(1), 129. https://doi.org/10.1186/s12936-023-04554-x
Crickmore, N., Berry, C., Panneerselvam, S., Mishra, R., Connor, T. R., & Bonning, B. C. (2021). A structure-based nomenclature for Bacillus thuringiensis and other bacteria-derived pesticidal proteins. Journal of Invertebrate Pathology, 186, 107438. https://doi.org/10.1016/j.jip.2020.107438
Darma, R., & Purnamasari, M. (2016). A Strong Antifungal-producing Bacteria from Bamboo Powder for Biocontrol of Sclerotium rolfsii in Melon (Cucumis melo var. Amanta). Journal of Plant Pathology & Microbiology, 07(02). https://doi.org/10.4172/2157-7471.1000334
Delgado-Baquerizo, M., Oliverio, A. M., & Brewer, T. E. (2018). A global atlas of the dominant bacteria found in soil. Science, 359(6373), 310–325. https://doi.org/https://doi.org/10.1126/science.aap9516
Dinas Kesehatan NTB. (2024). Kenali Demam Berdarah Dengue (DBD)—DINAS KESEHATAN PROVINSI NTB. https://dinkes.ntbprov.go.id/berita/kenali-demam-berdarah-dengue-dbd/
Dou, P., Cheng, Q., Liang, N., Bao, C., Zhang, Z., Chen, L., & Yang, H. (2023). Rhizosphere Microbe Affects Soil Available Nitrogen and Its Implication for the Ecological Adaptability and Rapid Growth of Dendrocalamus sinicus, the Strongest Bamboo in the World. International Journal of Molecular Sciences, 24(19), 14665. https://doi.org/10.3390/ijms241914665
Dulmage, T., Yousten, A. A., Singer, S., & Lacey, L. A. (1990). Guidelines for Production of Bacillus thuringiensis H-14 and Bacillus sphaericus. UNDP WHO Special Programme for Research and training in Tropical Diseases. https://iris.who.int/items/40e1a752-9080-41ea-9c53-74e75e8ac9da
El-Ghiet, U., Moustofa, S., Ayashi, M., El-Sakhawy, M., Ateya, A., & Waggiallah, H. (2023). Characterization of Bacillus thuringiensis isolated from soils in the Jazan region of Saudi Arabia, and their efficacy against Spodoptera littoralis and Aedes aegypti larvae. Saudi Journal of Biological Science, 30(103721), 9. https://doi.org/10.1016/j.sjbs.2023.103721
Fierer, N. (2014). Embracing the unknown: Disentangling the complexities of the soil microbiome. Nature Reviews Microbiology, 15(10), 579–590. https://doi.org/https://doi.org/10.1038/nrmicro.2017.87
Fouet, C., & Kamdem, C. (2019). Integrated Mosquito Management: Is Precision Control a Luxury or Necessity? Trends in Parasitology, 35(1), 1–17. https://doi.org/10.1016/j.pt.2018.10.004
Harapan, H., Michie, A., Yohan, B., Shu, P. Y., Mudatsir, M., Sasmono, R. T., & Imrie, A. (2019). Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades. Reviews in Medical Virology, 29(4), e2037. https://doi.org/10.1002/rmv.2037
Hassan, A., Youssef, M., Elashtokhy, M., Ismail, I., Aldayel, M., & Afkar, E. (2021). Isolation and identification of Bacillus thuringiensis strains native of the Eastern Province of Saudi Arabia. Egyptian Journal of Biological Pest Control, 31(1), 1–6. https://doi.org/10.1186/s41938-020-00352-8
Kemenkes. (2024). Waspada Penyakit di Musim Hujan. Kemenkes Hebat, Indonesia Sehat. https://kemkes.go.id/id/waspada-penyakit-di-musim-hujan#:~:text=Pada%20minggu%20ke-43%20tahun,ke-43%20mencapai%20624.194%20suspek.
Liang, K. W. (2024). The Role of Leaf Litter in Forest Soil Fertility and Microbial Diversity. Molecular Soil Biology. Molecular Soil Biology, 15(3): 99-108. https://doi.org/10.5376/msb.2024.15.0011
Permata, A. H. D., Purnawati, A., & Suharto. (2024). Eksplorasi isolasi dan identifikasi bakteri antagonis dari lapisan rizosfer tanaman bambu (Bambusoideae sp.). Eksplorasi isolasi dan identifikasi bakteri antagonis dari lapisan rizosfer tanaman bambu (Bambusoideae sp.), 1, 469–481. https://prosiding.umy.ac.id/semnas-datan/index.php/dt/article/download/44/44/103
Prescott, L., & Harley, J. (2020). Laboratory Exercises in Microbiology (5th ed.). McGraw Hill Companies. https://www.researchgate.net/profile/Alaa-Niamah/post/Hi-everyone-does-anyone-have-protocols-for-the-following-tests/attachment/59d61d9779197b80779782f7/AS%3A271748644179985%401441801334827/download/Laboratory+Exercises+in+Microbiology+5thEd.pdf
Richards, S. L., Byrd, B. D., Reiskind, M. H., & White, A. V. (2020). Assessing Insecticide Resistance in Adult Mosquitoes: Perspectives on Current Methods. Environmental Health Insights, 14, 1178630220952790. https://doi.org/10.1177/1178630220952790
Soberón, M., Pardo-López, L., Muñóz-Garay, C., Gómez, I., & Gill, S. S. (2021). Bacterial Toxins Active against Mosquitoes: Mode of Action and Resistance. Toxins, 13(8), 566. https://doi.org/ 10.3390/toxins13080566
Yuan, Z. S., Liu, F., Liu, Z. Y., Huang, Q. L., Zhang, G. F., & Pan, H. (2021). Structural variability and differentiation of niches in the rhizosphere and endosphere bacterial microbiome of moso bamboo (Phyllostachys edulis). Scientific Reports, 11, 1574. https://doi.org/10.1038/s41598-021-80971-9
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