Heterogeneity of M5 Red Rice Mutants Irradiated With 200 and 300 Gy Based on Agronomic Traits Using Hierarchical Clustering Analysis
DOI:
10.29303/jbt.v26i3.13089Published:
2025-07-23Downloads
Abstract
Gamma-ray irradiation can broaden phenotypic variation in rice, making assessment of genotype heterogeneity important in advanced generations. This study evaluated the heterogeneity of M5 red rice mutants derived from 200 and 300 Gy irradiation based on agronomic traits using Hierarchical Clustering. The study involved 20 M5 mutant genotypes, comprising 10 genotypes from 200 Gy and 10 from 300 Gy, evaluated in three replications with Gamagora 7 as a check. Eleven agronomic traits were analyzed; maturity was excluded from clustering because all mutant genotypes had maturity of 107 days after transplanting. Genotype means were standardized and analyzed using Euclidean distance and Ward linkage. Cluster number was determined using silhouette, Calinski–Harabasz, Davies–Bouldin, and cophenetic correlation, while PCA was used to clarify variation. Five traits differed significantly: plant height, flowering time, maturity, grains per panicle, and filled grains per panicle. Three clusters provided the most informative structure, with a silhouette score of 0.324 and a cophenetic correlation coefficient of 0.732. The smallest Euclidean distance was 0.981 between G13 (12-2-8) and G63 (5-14-10), whereas the largest was 8.771 between G27 (1-20-19) and G27 (17-10-12). Internal dispersion was higher at 300 Gy than at 200 Gy, although multivariate centroids did not differ significantly between doses.
Keywords:
red rice; phenotypic heterogeneity; gamma irradiation; M5; Hierarchical Clustering; PCA.References
Faiqon, M. M., Supriyanta, & Wulandari, R. A. (2017). Pendugaan parameter genetik komponen hasil untuk seleksi tidak langsung tanaman padi (Oryza sativa L.) berdaya hasil tinggi. Vegetalika. https://doi.org/10.22146/veg.26168
Hidayat, R., & Adiredjo, A. L. (2020). Keragaman genetik dan heritabilitas beberapa karakter kuantitatif pada populasi tanaman padi (Oryza sativa L.) generasi F2. Jurnal Produksi Tanaman, 8(1), 99–105. https://doi.org/10.21176/protan.v8i1.1325
Kartina, N., Wibowo, B. P., Rumanti, I. A., & Satoto. (2017). Korelasi hasil gabah dan komponen hasil padi hibrida. Jurnal Penelitian Pertanian Tanaman Pangan, 1(1), 11–19. https://doi.org/10.21082/jpptp.v1n1.2017.p11-19
Mba, C. (2013). Induced mutations unleash the potentials of plant genetic resources for food and agriculture. Agronomy, 3(1), 200–231. https://doi.org/10.3390/agronomy3010200
Nugroho, K., Trikoesoemaningtyas, Syukur, M., & Lestari, P. (2022). Analisis keragaman genetik karakter morfologi populasi M2 cabai hasil iradiasi sinar gamma. Jurnal Agronomi Indonesia, 49(3), 273–279. https://doi.org/10.24831/jai.v49i3.38448
Rachmawati, T., Yunus, A., & Parjanto. (2023). Produktivitas dan kandungan protein beras mutan M6 hasil iradiasi sinar gamma pada benih padi varietas Mentik Susu. Agrotechnology Research Journal, 7(1), 34–40. https://doi.org/10.20961/agrotechresj.v7i1.73844
Sao, R., Sahu, P. K., Patel, R. S., Das, B. K., Jankuloski, L., & Sharma, D. (2022). Genetic improvement in plant architecture, maturity duration and agronomic traits of three traditional rice landraces through gamma ray-based induced mutagenesis. Plants, 11(24), 3448. https://doi.org/10.3390/plants11243448
Samudin, S., Made, U., Mustakim, Samsudiar, & Ferianti, V. (2022). Analisis keragaman genetik dan heritabilitas beberapa kultivar padi gogo lokal. Jurnal Agrotech, 12(2), 53–56. https://doi.org/10.31970/agrotech.v12i2.92
Setiawan, B., Wijaya, I. N., & Lestari, D. A. (2022). Pengaruh genotipe terhadap pertumbuhan tinggi tanaman dan hasil beberapa varietas padi. Jurnal Ilmu Pertanian Indonesia, 27(3), 210–218. https://doi.org/10.18343/jipi.27.3.210
Suliartini, N. W. S., Aryana, I. G. P. M., Sudharmawan, A. A. K., & Sudika, I. (2022). Kandidat galur unggul mutan padi G16 hasil induksi mutasi dengan sinar gamma. Jurnal Sains Teknologi & Lingkungan, 8(1), 66–72. https://doi.org/10.29303/jstl.v8i1.293
Suliartini, N. W. S., Sapitri, M., Sudika, I. W., Aryana, I. G. P. M., & Sudharmawan, A. A. K. (2022). Karakterisasi dan keragaman genetik mutan padi Inpago Unram 1 generasi kedua (M2) akibat iradiasi sinar gamma. Jurnal Sains Teknologi & Lingkungan, 8(2), 124–136. https://doi.org/10.29303/jstl.v8i2.364
Sudharmawan, A. A. K. (2012). Perakitan galur harapan padi beras merah tipe ideal melalui seleksi pedigree. Laporan Penelitian Strategi Nasional, 45 hlm.
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