Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study
DOI:
10.29303/jbt.v21i1.2466Published:
2021-03-13Downloads
Abstract
Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.
ÃÂ
ÃÂ
Keywords:
Actinomycetes Antibacterial FiltrateReferences
Adhanti, R. (2012). Konsentrasi Efektif Ekstrak Daun Tembakau (Nicotiana Tabacum) Sebagai Pembersih Gigi Tiruan Resin Akrilik terhadap Jumlah Streptococcus Mutans. https://repository.unej.ac.id/handle/123456789/19381
Ambarwati, G. T. (2009). Isolasi aktinomycetes dari tanah sawah sebagai penghasil antibiotik. J Penel Sains Teknol, 10(2), 101-111. https://publikasiilmiah.ums.ac.id/bitstream/handle/11617/437/1.%20AMBARWATI.pdf?sequence=1&isAllowed=y
Barka, E. A., Vatsa, P., Sanchez, L., Gaveau-Vaillant, N., Jacquard, C., Klenk, H. P., ... & van Wezel, G. P. (2016). Taxonomy, physiology, and natural products of Actinobacteria. Microbiology and Molecular Biology Reviews, 80(1), 1-43. Available from: https://mmbr.asm.org/content/80/1/1.short
Brooks, GF, Janet, SB, Stephen A. Jawetz, Melnick & Adelbergs (2007). Mikrobiologi Kedokteran. jakarta: Penerbit Buku Kedokteran EGC; 2007.
Ceylan, Ãâ., Ãâkmen, G., & Ußur, A. (2008). Isolation of soil Streptomyces as source antibiotics active against antibiotic-resistant bacteria. http://acikerisim.mu.edu.tr/xmlui/handle/20.500.12809/7761
Davis, W. W., & Stout, T. R. (1971). Disc plate method of microbiological antibiotic assay: I. Factors influencing variability and error. Applied microbiology, 22(4), 659-665. https://aem.asm.org/content/22/4/659.short
Fatoni, J., Yuliani, R., & St, M. B. (2016). Uji Potensi Antibakteri Isolat Rare Actinomycetes Material Pasir Pantai Baron Gunung Kidul Yogyakarta terhadap Bakteri Pseudomonas aeruginosa (Doctoral dissertation, Universitas Muhammadiyah Surakarta). http://eprints.ums.ac.id/45796/
Hamzah, A. (2019). Analisis In Vitro Aktivitas Antibakteri Daun Sisik Naga (Drymoglossum Pilosellaoides) Terhadap Bakteri Vibrio Harveyi Dan Vibrio Parahaemolyticus. Journal of Aquaculture and Fish Health, 8(2), 86-91.
Hasyim, A., & Tulak, Y. F. (2013). Isolasi dan Karakterisasi Actinomycetes Sebagai Penghasil Antibiotik Dari Sampel Tanah Pada Peternakan Sapi di Kecamatan Galesong Kabupaten Takalar. Biogenesis: Jurnal Ilmiah Biologi, 1(2), 97-100. http://103.55.216.56/index.php/biogenesis/article/view/454
Harvey C, Cornellissen CN, Fisher BD, AR. (2013). Lippincottââ¬â¢s illustrated reviews Pharmacology 7th Edition pdf Free Download - Free Medical Books [Internet]. [cited 2020 Jul 29]. Available from: https://mdlecture.blogspot.com/2019/11/lippincotts-illustrated-reviews-pdf-Free-Download.html
Kurniawan DC (2017). Daya Hambat Infusa .Batang Bidara Laut.(Strychnosingustrina Blume) terhadap .Bakteri Staphylococcusauereus dan .Escherichia coli. .Universitas .Muhammadiyah .Semarang ââ¬Ë[Internet]. Available .from: .http://repository.unimus.ac.id/12..64/
Leetanasaksakul K, Thamchaipenet A. (2018). Potential anti-biofilm producing marine actinomycetes isolatd from sea sediments in Thailand. Agric Nat Resour [Internet]. 52 (3):228ââ¬â33. Available from: https://doi.org/10.1016/j.anres.2018.09.003
Masda NR. (2018). Potensi Metabolit Sekunder Isolat Actinomycetes Sm-2 Dari Rizosfer Andrographis Paniculata Sebagai Penghasil Senyawa Antibakteri. Universitas Hassanuddin Makassar [internet]. Available from: http://digilib.unhas.ac.id/uploaded_files/temporary/DigitalCollection/NmRkYmQ2NmI4ZDUxM2I5MzFmNGM5OGI1NjZmNjk3YzY4NjM2MDQ2NA==.pdf
Morse AS, Brooks GF, Butel JS. Jawetz Melnick & Adelbergs (2015). Medical Microbiology 27 E (Lange): 9780071824989: Medicine & Health Science Books @ Amazon.com [Internet]. [cited 2020 Jul 29]. Available from: https://www.amazon.com/Jawetz-Melnick-Adelbergs-Medical-Microbiology/dp/0071824987
NUR AA. (2013). Isolasi Mikroba Penghasil Antibiotika Dari Tanah Sekitar Pembuangan Limbah Pabrik Gula Takalar. Universitas Hasanuddin Makassar [Internet]. Avaible from: http://digilib.unhas.ac.id/uploaded_files/temporary/DigitalCollection/OTA0ZmY2YTM3ZGQyYzZmMTZlZjY2Y2RmZjY1YzgzODE4MWMzNGEwNQ==.pdf
Nurkanto A. (2012). Penapisan Actinomycetes Asal Raja Ampat, Papua Penghasil Antimikroba Dan Antikanker, Dan Identifikasi Molekuler Isolat-Isolat Terpilih. Universitas Indonesia [Internet]. Available from: http://lib.ui.ac.id/file?file=digital/20376144-T40830-Arif%20Nurkanto.pdf
Pratiwi S. (2008). Uji Efektivitas Ekstrak Daun Cincau Hijau Rambat (Cyclea Barbata Miers.) Sebagai Antibakteri Terhadap Bacillus Cereus Dan Shigella Dysenteriae Secara In Vitro Dengan Metode Difusi. Repository UPN Veteran Jakarta [Internet]. [cited 2020 Jul 29]. Available from: http://repository.upnvj.ac.id/2025/
Pujiati P. (2014). Isolasi Actinomycetes Dari Tanah Kebun Sebagai Bahan Petunjuk Praktikum Mikrobiologi. Florea, J Biol dan Pembelajarannya [Internet]. 1 (2):42ââ¬â6. Available from: DOI: 10.25273/florea.v1i2.390
Subramani R, & Aalbersberg W. (2013). Culturable rare Actinomycetes: Diversity, isolation and marine natural product discovery. Appl Microbiol Biotechnol [Internet]. [cited 2020 Jul 29];97(21):9291ââ¬â321. Available from: https://pubmed.ncbi.nlm.nih.gov/24057404/
Talukdar M, Bordoloi M, Dutta PP, Saikia S, Kolita B, Talukdar S, et al. (2016). Structure elucidation and biological activity of antibacterial compound from Micromonospora auratinigra , a soil Actinomycetes. J Appl Microbiol [Internet]. [cited 2020 Jul 30]; 121 (4):973ââ¬â87. Available from: http://doi.wiley.com/10.1111/jam.13233
License
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
