The Effect Hormone BAP (6-Benzyl Amino Purine) on the Growth of Potato Axillary Shoots (Solanum Tuberosum L.) in Vitro
DOI:
10.29303/jbt.v21i3.2823Published:
2021-09-13Downloads
Abstract
Potatoes are one of the foodstuffs that contain lots of carbohydrates, minerals, and vitamins. To develop potato cultivation, superior varieties are needed that produce high yields, are resistant to pests, and have good tuber quality. The growth of potato plantlets in the in vitro subculture process is determined by many factors, one of which is the administration of growth regulators and the concentration of growth regulators. The type of media or nutrients used also affects the growth and development of the explants and seedlings produced. This study aims to trigger the growth of potato plantlets so that their growth is optimal. The method used is a true experimental quantitative method with a one-factor Completely Randomized Design (CRD) design, namely variations in BAP concentration consisting of 5 treatments with 5 replications. Data collection techniques through observation or observation. Parameters observed were number of shoots, number of leaves, number of roots, and plantlet height. The data analysis technique used is the one way ANOVA test and for the average difference between treatments, the HSD (Honestly Significant Difference) test at 5% level is used. Based on the results of the study, it was known that there was an effect of giving the hormone BAP (6-Benzyl Amino Purine) on the growth of potato axillary shoots (Solanum tuberosum L.) in vitro. The results showed that there was a significant effect on the number of shoots (P = 0.007) with an average of 1.64 pieces, the number of leaves (P = 0.002) with an average of 1.42 pieces, the number of roots (P = 0.000) with an average of 1. an average of 1.70 pieces, and plantlet height (P=0.000) with an average of 2.65 cm. It was concluded that treatment D (1.5 ppm BAP) was the best treatment in increasing the growth of potato plantlets.
Keywords:
BAP hormone in vitro culture potato axillary shootsReferences
Abidin, Z. (1983). Dasar-Dasar Pengetahuan Tentang Zat Pengatur Tumbuh. Bandung: Angkasa.
Ai, N. S., & Paatricia. (2013). Karakter Morfologi Akar Sebagai Indikator Kekurangan Air Pada Tanaman (Root Morphological Characters As Water-Deficit Indicators In Plants) 1). Jurnal Bioslogos, 3(1), 31ââ¬â29.
Dewanto, Hamami Alfasani. Saraswati, D. & Hadjoeningtijas, O. D. (2018). Pertumbuhan Kultur Tunas Aksilar Kentang (Solanum tuberosum L.) Dengan Penambahan Super Fosfat dan KNO3 pada Media AB Mix Secara In Vitro. Universitas Muhammadiyah Purwokerto, XX.
Hapsoro, D., & Yusnita. (2018). Kultur Jaringan. Yogyakarta: ANDI.
Inkiriwang, A. E. B., Mandang, J., Agronomi, S., Sarjana, P., & Sam, U. (2016). Substitusi Media Murashige and Skoog/MS dengan Air Kelapa dan Pupuk Daun Majemuk pada Pertumbuhan Anggrek Dendrobium Secara In Vitro. UNSRAT: Manado. 6(1): 17-19.
Irvandi, D., & Nurbaiti. (2017). Pengaruh Pupuk Npk Dan Air Kelapa Sebagai Zat Pengatur Tumbuh Alamiterhadap Pertumbuhan Bibit Kakao (Theobroma Cacao L.) Di Medium Sub Soil. JOM Faperta UR, 4(2), 1ââ¬â12.
Lestari, F. W., Suminar, E., & Mubarok, S. (2018). Pengujian Berbagai Eksplan Kentang (Solanum tuberosum L.) dengan Penggunaan Konsentrasi BAP dan NAA yang Berbeda In Vitro Test Of Various Potato (Solanum Tuberosum L.) Explants With The Use Of Different Cytokinins And Auxins. 5(1), 66ââ¬â75.
Molla, M. M. H., Nasiruddin, K. M., Khanam, D., & Salam, M. A. (2011). Effect Of Growth Regulators On Direct Regeneration Of Potato. In International Conference On Environment And Industrial Innovation. Singapore: IACSIT Press., 12, 205ââ¬â210.
Muliawati, Et Al. (2017). Aklimatisasi Planlet Pisang Varietas Raja Bulu Kuning Berbasis Sistem Hidroponik Substrat. Jurnal Agrotech Res J, 1(2), 1ââ¬â6.
Nida, R. (2018). Perbandingan Pertumbuhan Anggrek Dendrobium nobile Linn. Menggunakan Media Subkultur dengan Penambahan Ekstrak Buah Pisang Ambon dan Ekstrak Buah Nangka. Skripsi. Program Studi Pendidikan Biologi. Universitas Sanata Dharma Yogyakarta.
Nuryadin, E., Sugiyono, S., & Proklamasiningsih, E. (2017). Pengaruh Zat Pengatur Tumbuh Terhadap Multiplikasi Tunas Dan Bahan Penyangga Pada Pembentukan Plantlet Kantong Semar AdrianiI (Nepenthes Adrianii) dengan Kultur In Vitro. Bioeksperimen: Jurnal Penelitian Biologi, 3(2), 31. Https://Doi.Org/10.23917/Bioeksperimen.V3i2.5180
Rahmah, V.N., Suprapto, P.W & Nuryadin, E. (2021). Media Ekstrak Buah Untuk Pertumbuhan Planlet Anggrek Vanda Tricolor Secara In Vitro. Jurnal Metamorfosa, 8(1): 131-140.
Sari, D. A., Restanto, D. P., Pertanian, F., Unej, U. J., Kalimantan, J., & Boto, K. T. (N.D.). Pertanian Induksi Tunas Kentang (Solanum Tuberosum L.) menggunakan BAP ( Benzil Amino Purine ). X, 1ââ¬â4.
Shanti, V. . (2005). Pengaruh Konsentrasi Paclobutrazol dan 6-Benzil Amino Purine (BAP) terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L) pada Media MS Secara In Vitro. Skripsi S1 FP.UNS.
Sitompul, S. M., & Guritno, B. (1995). Analisis Pertumbuhan Tanaman. Yogyakarta: Gadjah Mada University Press.
Wattimena, G.A. (1986). Kultur Jaringan Tanaman Kentang. Makalah dalam training course on potato seed technology. Dir. Bina Prod. Hort. FAO. 27 Oct-8 Nov 1986.
Wirawan, D. (2003). Pengaruh Konsentrasi Bap (6-Benzyl Amino Purin) Terhadap Pertumbuhan (Kultur In Vitro) Mahkota Dewa (Phaleria Macrocarpa Scheff. Boerl.). IPB University.
Wroblewska, K. (2013). Benzyladenine Effect on Rooting and Axillary Shoot Outgrowth of Gaura lindheimeri Engelm. A. Gray Cuttings. Acta Sci.Pol., Hortorum Cultus. 12(3): 127-136.
Yusnita. (2015). Kultur Jaringan Tanaman Sebagai Teknik Penting Bioteknologi Untuk Menunjang Pembangunan Pertanian. Penerbit Aura Publishing, 1ââ¬â86.
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