Isolation and Analysis of Bioactive Compounds Endophytic Bacteria of Sea Fern (Acrtostichum aureum L.) from Bengkalis Island, Riau
DOI:
10.29303/jbt.v22i1.3104Published:
2021-12-24Downloads
Abstract
Endophytic bacteria have been known to produce high levels of secondary metabolites. The sterile leaves of the sea fern (Acrostichum aureum L.) contain many phytochemical compounds. This sea fern is often found in peat swamp areas. The aim of this study was to isolate, partial characterization, and analysis of bioactive compounds from endophytic bacteria of sea ferns to determine their bioactive compounds. Endophytic bacteria were isolated by a direct technique using nutrient agar (NA) media. In this study, the endophytic bacteria were characterized by macroscopic, microscopic, and Gram. Several tests were further performed, namely phytochemical test and Fourier Transform Infra Red (FT IR) FTIR tests from a crude extract of secondary metabolites of bacteria. The results of the isolation obtained six isolates of endophytic bacteria consisting of all isolates were Gram-negative. The phytochemical test results for the six isolates were positive for alkaloids and saponins but no steroids. Based on the results of phytochemical tests and FT IR, it is suspected that the isolate DSB 1.6 has similarities with the alkaloid functional group. This endophytic bacterial isolate can be developed as a candidate for produced of pharmacological compounds.Keywords:
Endophytic bacteria Peat swamp Phytochemical Sea fernReferences
Anwar L & Futra D. (2019). Potensi Metabolit Sekunder Produksi Bakteri Endofit dari Tumbuhan Laban (Vitex pubescens Vahl) Sebagai Antikanker. Chempublish Journal. 4 (2): 71-80. DOI:10.22437/chp. v4i2.7937.
Aristina RF, Astuti W & Pratiwi DR. (2019). Skrining dan Uji Fitokimia Ekstrak Kasar Bakteri Endofit dari Batang Pacing (Costus sp). Jurnal Atomik 4(1): 21-24. DOI: https://doi.org/10.35799/jm.7.2.2018.20640.
Asmoro PP & Munif A. (2019). Bakteri Endofit dari Tumbuhan Paku-pakuan sebagai Agens Hayati Rhizoctonia solani dan Pemacu Pertumbuhan Tanaman Padi. Jurnal Fitologi Indonesia. 15(6): 239-247. DOI: https://doi.org/10.14692/jfi.15.6.239-247
Badhsheeba, MA & Vadivel V. (2020). Physicochemical and Phytochemical Contents of the Leaves of Acrostichum aureum L. Journal of Global Biosciences. 9(4): 7003-7018. DOI: www.mutagens.co.in/jgb/vol.09/04/090407.pdf
Chen XH, He ZB, Li F, L SH, Li FN, Chen MS, Wu YL & Tuo L. (2021). Nocardioides acrostichi sp. nov., a Novel Endophytic Actinobacterium Isolated from Leaf of Acrostichum aureum. Antonie van Leeuwenhoek. 114:479ââ¬â486. https://doi.org/10.1007/s10482-021-01535-5.
Chigurapati S, Vijayabalan S, Karunanidhi A,
Selvarajan KK, Nanda SS & Satpathy R. (2019). Antidiabetic, Antioxidant and in Silico Studies of Bacterial Endosymbiont Inhabiting Nephelium lappaceum. Ovidius University Annals of Chemistry. 30 (2): 95-100. DOI:10.2478/auoc-2019-0017
Fatin N, Pujiyanto S & Raharjo B. (2018). Uji aktivitas Inhibisi ñ-Glukosidase Isolat Bakteri Endofit Tanaman Duwet (Syzygium cumini L. Skeels) sebagai Sumber Alternatif Antidiabetes. Jurnal Bioma. 20 (2): 165-169. DOI.org/10.14710/bioma.20.2.165-169
Hanin NNF & Pratiwi R. (2017). Kandungan Fenolik, Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Paku Laut (Acrostichum aureum L.) Fertil dan Steril. Journal of Tropical Biodiversity and Biotechnology. 2: 51-56. DOI.org/10.22146/jtbb.29819
Haryani Y, Hilma R, Delfira N, Linda TM, Puspita F, Friska A, Juwita D, Farniga A & Ardi F. (2019). Potential Antibacterial Activity of Endophytic Fungi Penicillium sp. and Trichoderma sp. Derived From Mangrove Ceriops Tagal (Perr.) C.B.Robb and Bruguiera sp. Journal of Physics Conference Series 1351:012100. DOI: 10.1088/1742-6596/1351/1/012100.
Haryani Y, Hilma R, Delfira N, Linda TM, Puspita F, Friska A, Juwita D, Farniga A & Ardi F. (2020). Antibacterial activity of Achromobacter sp. and Bacillus sp., bacterial endophytes derived from Mangrove Ceriops tagal (Perr.) C.B.Robb. IOP Conf. Ser.: Mater. Sci. Eng. 833 012013. DOI:10.1088/1757-899X/833/1/012013.
Khan SA, Hossain MA, Panthi S, Asadujjaman M & Hossin A. (2013). Assessment of Antioxidant and Analgesic Activity of Acrostichum aureum Linn. (Family-Pteridaceae). Pharmacologyonline 1: 166-171. https://www.semanticscholar.org/paper/Assessment-of-antioxidant-and-analgesic-activity-of-Khan-ossain/27cf2742ab9b6d99b14012d45cea1e55e8612b58.
Kobayashi DY & Palumbo JD. (2000). Bacterial endophytes and their effects on plants and uses in agriculture. In: Bacon CW, White JF (eds.), Microbial Endophytes. pp 199ââ¬â233. ISBN: 9780429179334Marcel Dekker, Inc. New York, N.Y.
Linda TM, Siregar S, Fitri WD, Martina A, Lestari W, Roslim DI & Hapsoh. (2018). Isolation and screening of culturable endophytic bacteria from leaf of rubber plant that produces of chitinase. Journal of Physics: Conf. Series 1116 ââ¬â 052038. DOI:10.1088/1742-6596/1116/5/052038
Lestari K, Agustien A & Djamaan A. (2019). The Potential of Endophytic Fungi Isolated from Leaves, Stems, Mangrove Roots Avicennia marina as a Producer of Antibiotics. Jurnal Metamorfosa 6 (1): 83-89). DOI: 10.24843/metamorfosa.v06.i01.p13
Maheshwari DK (Ed). (2017). Endophytes: Biology and Biotechnology Volume 1. India: Springer Nature. DOI 10.1007/978-3-319-66541-2
Marliana S, Suryanti V & Suyono (2005). Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol. Biofarmasi. 3 (1): 26-31. DOI: 10.13057/biofar/f030106
Matthew O, Olusola E , Ademola O, Aderotimi A & Adebola J. (2018). Anti-malarial Activity of Total Saponins from Terminalia avicennioides and Its Effect on Liver and Haematological of Infected Mice. Drug Des 2018, 7(2):1-6. DOI: 10.4172/2169-0138.1000161
Milanda T, Lestari K & Nimas TIT. (2021). Antibacterial Activity of Parijoto (Medinilla speciosa Blume) Fruit Against Serratia marcescens and Staphylococcus aureus. Indonesian Journal of Pharmaceutical Science and Technology 8(2): 76-85. DOI: https://doi.org/10.24198/ijpst.v8i2.32166
Mubekti (2011). Studi Pewilayahan Dalam Rangka Pengelolaan Lahan Gambut Berkelanjutan di Provinsi Riau. Jurnal Sains dan Teknologi Indonesia. 13 (2): 88-94. DOI: 10.29122/jsti.v13i2.883
Nasrul B. (2010). Penyebaran dan potensi lahan gambut di Kabupaten Bengkalis untuk pengembangan pertanian. Jurnal Agroteknologi 1 (1): 1-7. http://ejournal.uin-suska.ac.id/index.php/agroteknologi/article/view/5/5
Photolo MM, Mavumengwana V, Sitole L, & Tlou MG. (2020). Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754 Isolated from Combretum erythrophyllum Seeds. International Journal of Microbiology. 1-11. DOI.org/10.1155/2020/9483670
Pujiyanto S, Resdiani M, Raharja B & Ferniah RS. (2018). ñ-Amylase Inhibitor Activity of Endophytic Bacteria Isolated from Annona muricata L. Journal of Physics: Conference Series 1025. DOI: 10.1088/1742-6596/1025/1/012085
Raja S & Ravindranadh K. (2014). Preliminary Phytochemical Screening of Different Solvent Extracts of Whole Plant of Acrostichum aureum. World Journal of Pharmaceutical Sciences. 2 (12): 1753-1759. http://www.wjpsonline.org/view_issue.php?title=Preliminary-phytochemical-screening-of-different-solvent-extracts-of-whole-plant-of-Acrostichum-aureum
Romadhon, Subagiyo & Margino S. (2012). Isolasi dan Karakterisasi Bakteri Asam Laktat dari Usus Udang Penghasil Bakteriosin Sebagai Agen Antibakteria pada Produk-Produk Hasil Perikanan. Jurnal Saintek Perikanan 8 (1):59-64. DOI: https://doi.org/10.14710/ijfst.8.1.59-64
Rori CA, Kandou FEF & Tangapo AM. (2020). Isolasi dan Uji Antibakteri Dari Bakteri Endofit Tumbuhan Mangrove Avicennia marina. Jurnal Koli. 1 (1):1-7. https://ojs3.unpatti.ac.id/index.php/koli/issue/view/355
Rusli & Rahmaniar D. (2013). Penelusuran Potensi Mikroba Endofit dari Rimpang Paku Kepala Tupai (Drynaria quercifolia J.Smith) sebagai Penghasil Senyawa Antibiotika. As-syifa Jurnal Farmasi. 5 (2): 128-139. https://jurnal.farmasi.umi.ac.id/index.php/as-syifaa/issue/view/10
Setyaningrum E, Arifiyanto A, Nukmal N. (2021). In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp hygroscopicus Strain i18 Isolated from a Pineapple Farm in Lampung, J Pure Appl Microbiol. 15(2):891-896. DOI.org/10.22207/JPAM.15.2.45
Sofiyanti N, Isda MN, Juliantari E, Suriatno R, & Pratama S. (2019). The Inventory and Spore Marphology or Ferns From Bengkalis Island, Riau Province, Indonesia. Jurnal Biodiversitas. 20 (11): 3223-3236. DOI.org/10.13057/biodiv/d201115
Skoog DA, Holler FJ & Crouch SR. (2007). Principles of Instrumental Analysis. Canada: David Harris Publisher. pp 455-475. ISBN-13: 978-0-495-01201-6
Suhartina, Kandou FEF & Singkoh MFO. (2018). Isolasi dan Identifikasi Jamur Endofit Pada Tumbuhan Paku Asplenium nidus. Jurnal FMIPA 7(2): 24-28. DOI: https://doi.org/10.35799/jm.7.2.2018.20640
Tuo L & Yan XR. (2019). Aureimonas flava sp. nov., a Novel Endophytic Bacterium Isolated from Leaf of Acrostichum aureum. Int J Syst Evol Microbiol. 69:846ââ¬â851. DOI 10.1099/ijsem.0.003252.
Triana O, Sarjono PR, & Mulyani NS. (2017). Isolasi Bakteri Endofit Pada Rimpang Jahe Merah (Zingiber offcinale Linn. Var Rubrum) Penghasil Senyawa Antioksidan. Jurnal Kimia Sains dan Aplikasi. 20 (1): 25-29. DOI.org/10.14710/jksa.20.1.25-29
Variani YA, Setyaningrum E, Handayani K, Nukmal N & Arifiyanto A. (2021). Analisis Senyawa Bioaktif Ekstrak Metabolit Sekunder Serratia marcescens strain MBC1. Ind. J. Chem. Anal. 4 (2): 64-71. DOI: 10.20885/ijca.vol4.iss2.art3.
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