Inhibition of bacterial growth of Leilem leaf extract (Clerodendrum minhassae Teijsm. & Binn)
Inhibition of bacterial growth of Leilem leaf extract
Authors
Masje Wurarah , Yermia Semuel MokosuliDOI:
10.29303/jbt.v22i2.3394Published:
2022-05-26Issue:
Vol. 22 No. 2 (2022): April - JuneKeywords:
Leilem leaves; antibacteria; extract.Articles
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Abstract
Leilem (Clerodendrum minhassae Teism. & Binn.) is a plant endemic to Sulawesi. Leilem leaves are used as a typical food vegetable for the Minahasa tribe. However, leilem leaves were used as a medicinal plant for intestinal worms, abdominal pain and chest pain. There has been no research report on the use of leilem leaves as a source of antibacterial bioactivity. This study aims to obtain the best concentration of antibacterial Leilem leaf extract. Leilem leaves are obtained from North Minahasa. Extraction by maceration method using various solvents with a level of polarity. The test concentrations of the extract were 100 mg/L, 200 mg/L, 400 mg/L and 800 mg/L. As a positive control, clindamycin 400 mg/L was used. The results showed that the percentage of solvent yield was ethanol, which was 1.23% at 1:4 (w/v) maceration. The average diameter of bacterial growth is 12.6 mm. The results of the one way ANOVA analysis showed significant differences between test concentrations (p>0.5). Tukey's test showed that the three extracts significantly inhibited the growth of gram-positive bacteria Staphylococcus aureus strain ATCC 25923. The n-hexane extract showed the best antibacterial activity, followed by ethanol extract and ethyl acetate extract. Leilem leaves are potential to be developed as a source of antibacterial bioactive.
References
Adam, C., Djarkasi, G. S., Ludong, M. M., & Langi, T. (2013, April). Penentuan total fenol dan aktivitas antioksidan ekstrak Daun Leilem (Clerodendrum minahassae). In COCOS (Vol. 2, No. 3).
Baroroh, H. N., Utami, E. D., Maharani, L., & Mustikaningtias, I. (2018). Peningkatan Pengetahuan Masyarakat Melalui Edukasi Tentang Penggunaan Antibiotik Bijak dan Rasional. ad-Dawaa'Journal of Pharmaceutical Sciences, 1(1).
Bontjura, S., Waworuntu, O. dan Siagian, K., 2015, Uji Efek Antibakteri Ekstrak Daun Leilem (Clerodendrum minahassae L.) Terhadap Bakteri Streptococcus mutans, Pharmacon Jurnal Ilmiah Farmasi, Manado, Indonesia, hal.99'
Bontjura et al., (2015) meneliti bahwa senyawa fenol dalam ekstrak daun leilem memiliki efek antibakteri terhadap bakteri Streptococcus mutans.
Francis, G., Kerem, Z., Makkar, H. P., & Becker, K. (2002). The biological action of saponins in animal systems: a review. British journal of Nutrition, 88(6), 587-605.
Górniak, I., Bartoszewski, R., & Króliczewski, J. (2019). Comprehensive review of antimicrobial activities of plant flavonoids. Phytochemistry Reviews, 18(1), 241-272.
Gorlenko, C. L., Kiselev, H. Y., Budanova, E. V., Zamyatnin, A. A., & Ikryannikova, L. N. (2020). Plant secondary metabolites in the battle of drugs and drug-resistant bacteria: new heroes or worse clones of antibiotics?. Antibiotics, 9(4), 170.
Harborne, J.B. (1987). Metode Fitokimia, Penuntun Cara Modern Menganalisis Tumbuhan, terbitan kedua,ITB. Bandung, Indonesia.
Hamidu, L., Ahmad, A. R., & Najib, A. (2018). Qualitative and quantitative test of total flavonoid buni fruit (Antidesma bunius (L.) Spreng) with UV-Vis spectrophotometry method. Pharmacognosy Journal, 10(1).
Kongkham, B., Prabakaran, D., & Puttaswamy, H. (2020). Opportunities and challenges in managing antibiotic resistance in bacteria using plant secondary metabolites. Fitoterapia, 147, 104762.
Kaunang, E. S. N. and Mokosuli Y. S. (2017). Botanical and phytochemical constituents of several medicinal plants from mount Klabat north Minahasa. J Med Plants Stud; 5(2):29-35.
Kairupan, C. F., Mantiri, F. R., & Rumende, R. R. H. (2019). Phytochemical screening and antioxidant activity of ethanol extract of leilem (Clerodendrum minahassae Teijsm. & Binn) as an antihyperlipidemic and antiatherosclerotic agent. In IOP Conference Series: Earth and Environmental Science (Vol. 217, No. 1, p. 012016). IOP Publishing.
Kaunang E. S. N. and Mokosuli Y. S. Botanical and phytochemical constituents of several medicinal plants from mount Klabat north Minahasa. J Med Plants Stud 2017; 5(2):29-35.
Lopatkin, A. J., Bening, S. C., Manson, A. L., Stokes, J. M., Kohanski, M. A., Badran, A. H., ... & Collins, J. J. (2021). Clinically relevant mutations in core metabolic genes confer antibiotic resistance. Science, 371(6531), eaba0862.
Leeratiwong, C., Chantaranothai, P., & Paton, A. J. (2011). A synopsis of the genus Clerodendrum L. (Lamiaceae) in Thailand. Tropical Natural History, 11(2), 177-211.
Mokosuli, Y. S. (2008). Aktivitas antioksidasi dan antikanker ekstrak kulit batang Langsat (Lansium domesticum L.) (Doctoral dissertation, Tesis).
Maria, R., Shirley, M., Xavier, C., Jaime, S., David, V., Rosa, S., & Jodie, D. (2018). Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province Ecuador. Journal of King Saud University-Science, 30(4), 500-505.
Negara, K. S. (2016). Analisis implementasi kebijakan penggunaan antibiotika rasional untuk mencegah resistensi antibiotika di RSUP Sanglah Denpasar: Studi kasus infeksi methicillin resistant Staphylococcus aureus. Jurnal Administrasi Rumah Sakit Indonesia, 1(1).
Situmorang, H. R. (2016). Uji konsentrasi hambat minimum (KHM) ekstrak daun leilem (Clerodendrum minahassae L.) terhadap bakteri Streptococcus mutans. PHARMACON, 5(4).
Semuel, M. Y., Kaunang, E. S. N., & Manopo, J. S. (2019). The bioactive contents and antioxidant activity of honey bee nest extract of Apis dorsata Binghami from the North Sulawesi. Molekul, 14(2), 92-102.
Rachmatiah, T., Daud, J. J., & Artanti, N. (2022). Aktivitas Antioksidan, Toksisitas, Kandungan Senyawa Fenol dan Flavonoid Total dari Daun Leilem (Clerodendrum minahassae Teijsm. & Binn). SAINSTECH FARMA, 15(1), 35-43.
Repi, R. A., Mokosuli, Y.S. Ngangi, J., & Sumampouw, H. M. (2013). Hepatoprotective Activity Combination between Morinda Citrifolia Linn (Mengkudu) Extract and Virgin Coconut Oil (VCO). Journal of Biology, Agriculture and Healthcare, 3(13), 160-166.
Rettob, T. M. K., Zebua, E., Butar-butar, I. S., Tular, F. G., & Mokosuli, Y. S. (2021). The Utilization of Beehive Wax a Combination of Nutmeg Extract (Myristica fragrans Houtt.) and Langsat (Lansium domesticum L.) as Aromatherapy and Mosquito Repellent. Jurnal Biologi Tropis, 21(3), 845-853.
Rombot, D. V., & Semuel, M. Y. (2020). Bioaktivitas Larvasida Nyamuk Anopheles sp. Dari Ekstrak Bunga Tagetes erecta L. Yang Berasal Dari Kota Tomohon. Jurnal Biomedik: JBM, 12(3), 161-167.
Utami, Y. P., Imrawati, I., & Rasyid, A. (2018). Isolasi dan Karakterisasi Ekstrak Etanol Daun Leilem (Clerodendrum Minahassae Teijsm Dan Binn.) Dengan Metode Spektrofotometri Isolation And Characterization Ethanol Extract Of Leilem Leaves (Clerodendrum Minahassae Teijsm And Binn) With Methods Spectrophotometric. Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ), 1(2).
Patle, T. K., Shrivas, K., Kurrey, R., Upadhyay, S., Jangde, R., & Chauhan, R. (2020). Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV–vis and FTIR spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 242, 118717.
Prasad, M. P., Sushant, S., & Chikkaswamy, B. K. (2012). Phytochemical analysis, antioxidant potential, antibacterial activity and molecular characterization of Clerodendrum species. International journal of molecular biology, 3(3), 71-76.
Venkatanarasimman, B., Rajeswari, T., & Padmapriya, B. (2012). Antibacterial potential of crude leaf extract of Clerodendrum philippinum Schauer. Int J Pharm Biol Arch, 3(2), 307-310.
Worang, R. L., Semuel, M. Y., & Pendong, D. F. (2013). Antimalarial and antibacterial bioactivity of langsat (Lansium minahasae l.) bark extract. Journal of Natural Sciences Research, 3(14).
Wikaningtyas, P., & Sukandar, E. Y. (2016). The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens. Asian Pacific Journal of Tropical Biomedicine, 6(1), 16-19.
Zaynab, M., Sharif, Y., Abbas, S., Afzal, M. Z., Qasim, M., Khalofah, A., ... & Li, S. (2021). Saponin toxicity as key player in plant defense against pathogens. Toxicon, 193, 21-27.
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