Isolation and Characterization of Mold on Furniture in Biological Laboratory Environment Using Contact Plate Method

Authors

Wahyu Aji Mahardhika , Romario Dion , Mochammad Fa’iq Qoys Naufal , Warih Ramadhany , Arina Tri Lunggani

DOI:

10.29303/jbt.v22i3.3416

Published:

2022-08-02

Downloads

Abstract

The presence of molds in the environment around us can be beneficial or detrimental. Mold can be found in various places, including soil, air, plants, animals, water, and on material surfaces. One of the roles of mold is as a bio-degradator and biodeterioration agent on materials that are still used, so that it will cause harm to humans. This study aims to isolate and characterize the types of molds that exist on the surface of existing materials in the biology laboratory environment of Diponegoro University with the contact plate method. The method used is to attach a contact plate containing PDA to various materials such as tables, chairs, cabinets, and so on, then observe the macroscopic and microscopic morphology. The results obtained were five genera, there are Aspergillus, Penicillium, Eurotium, Rhizopus, and Absidia.

Keywords:

Contact plate, isolation, mold, material

References

Adams, R. I., Miletto, M., Taylor, J. W., & Bruns, T. D. (2013). The Diversity and Distribution of Fungi on Residential Surfaces. Plos One, 8(11), E78866. doi: 10.1371/journal.pone.0078866

Ahmad, R. Z. (2017). Cemaran Kapang Pada Pakan Dan Pengendaliannya. Jurnal Litbang Pertanian. 28 (1). DOI: http://dx.doi.org/10.21082/jp3.v28n1.2009.p15%20-%2022

Ananso, G & Oje, N & Itoandon, Emoleila. (2018). Isolation And Characterization Of Most Probable Molds Associated With Some Damaged Materials. 12. 78-83. 10.9790/2402-1208017883.

Chunduri, J. (2014). Indoor Fungal Populations Inhabiting Cement Structures-Remedial Measures. IOSR Journal of Environmental Science, Toxicology and Food Technology, 8, 19-24. DOI: 10.9790/2402-08411924

Er, C. M., Sunar, N. M., Leman, A. M., Khalid, A., Ali, R., Zaidi, E., & Azhar, A. T. S. (2018). Indoor and Outdoor Surface-Growing Fungi Contamination at Higher Institutional Buildings in a Malaysian University. In IOP Conference Series: Earth And Environmental Science (Vol. 140, No. 1, P. 012118). IOP Publishing. doi :10.1088/1755-1315/140/1/012118

Gandjar, I., & Rifai, M. A. (1999). Pengenalan Kapang Tropik Umum. Yayasan Obor Indonesia.

Gao, X.-D., Katsumoto, T., & Onodera, K. (1995). Purification and Characterization of Chitin Deacetylase from Absidia coerulea. Journal of Biochemistry, 117(2), 257–263. Doi:10.1093/Jb/117.2.257 DOI: 10.1093/jb/117.2.257

Hussain, H., Kliche-Spory, C., Al-Harrasi, A., Al-Rawahi, A., Abbas, G., Green, I. R., Schulz, B. Krhon, K., Shah, A. (2014). Antimicrobial Constituents from Three Endophytic Fungi. Asian Pacific Journal of Tropical Medicine, 7, S224-S227. DOI: 10.1016/S1995-7645(14)60236-4

Islamiati, I., & Rahmawati, M. T. (2017). Jenis-Jenis Kapang Udara Ruang Baca Di UPT Perpustakaan Universitas Tanjungpura, Pontianak. Protobiont, 6(3). DOI: http://dx.doi.org/10.26418/protobiont.v6i3.22475

Jalgaonwala, R. E., & Mahajan, R. T. (2014). Production Of Anticancer Enzyme Asparaginase From Endophytic Eurotium Sp. Isolated From Rhizomes Of Curcuma Longa. Eur J Exp Biol, 4(3), 36-43.

Janda-Ulfig, K., Ulfig, K., & Markowska, A. (2009). Extracellular Enzyme Profiles Of Xerophilic Fungi Isolated From Dried Materials Of Medicinal Plants. Polish Journal of Environmental Studies, 18(3).

Klich, M. A. (2002). Identification of Common Aspergillus Species. CBS.

Liu, Z., Zhang, Y., Zhang, F., Hu, C., Liu, G., & Pan, J. (2018). Microbial Community Analyses Of The Deteriorated Storeroom Objects In The Tianjin Museum Using Culture-Independent And Culture-Dependent Approaches. Frontiers in Microbiology, 9, 802. doi: 10.3389/fmicb.2018.00802

López-Fernández, J., Benaiges, M. D., & Valero, F. (2020). Rhizopus oryzae Lipase, A Promising Industrial Enzyme: Biochemical Characteristics, Production and Biocatalytic Applications. Catalysts, 10(11), 1277. DOI:https://doi.org/10.3390/catal10111277

Poernomo, A. T., Isnaeni. Purwanto. (2015). Aktivitas Invitro Enzim Fibrinolitik Ekstrak Tempe Hasil Fermentasi Rhizopus oligosporus ATCC 6010 Pada Substrat Kedelai Hitam. Berkala Ilmiah Kimia Farmasi, 4(2), 18-24.

Precha, N., Kliengchuay, W., Woo, C., Yamamoto, N., & Tantrakarnapa, K. (2020). Fungal Assemblages on Indoor Surfaces with Visible Mold Growth In Homes After The 2016 Flood Disaster In Thailand. Applied Sciences, 10(15), 5322OI:https://doi.org/10.3390/app10155322

Samson, R. A., Hoekstra, E. S., & Frisvad, J. C. (2004). Introduction to Food-And Airborne Fungi (No. Ed. 7). Centraalbureau Voor Schimmelcultures (CBS).

Sudarma, I. D. G. A., Sastrawidana, I. D. K., & Maryam, S. (2017). Produksi Pigmen Warna Merah dari Jamur Penicillium purpurogenum yang Diisolasi dari Tanah Tercemar Limbah Susu Kambing Dengan Metode Submerged Fermentation. Wahana Matematika Dan Sains: Jurnal Matematika, Sains, Dan Pembelajarannya, 11(1), 19-32. DOI: http://dx.doi.org/10.23887/wms.v11i1.11844

Suryanto, D., Rahmiati, R., & Nurtjahja, K. (2011). Penapisan Jamur Penghasil Senyawa Antimikroba Dari Tanah Bangka Dan Taman Wisata Alam Sibolangit Serta Potensinya Menghambat Pertumbuhan Beberapa Jamur Patogen Tanaman. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 16(2), 362-370. DOI: https://doi.org/10.24002/biota.v16i2.120

Tri-Panji, Suharyanto, Paulus, A.W., Syamsu, K., Fauzi, A.M. (2002). Produksi Dan Stabilisasi Desaturase dari Absidia corymbifera. Menara Perkebunan. 70(2), 58-71. DOI: http://dx.doi.org/10.22302/iribb.jur.mp.v70i2.129

Utami AP, Setyaningsih R, Pangastuti A, Sari S.L.A. (2019). Optimasi Produksi Enzim Selulase Dari Jamur Penicillium Sp. SLL06 Yang Diisolasi Dari Serasah Daun Salak (Salacca edulis). Pros Sem Nas Masy Biodiv Indon 5: 145-149. DOI: 10.13057/psnmbi/m050201

Watanabe, T. (2010). Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi And Key To Species. CRC Press.

Yang, X., Kang, M. C., Li, Y., Kim, E. A., Kang, S. M., & Jeon, Y. J. (2014). Anti-Inflammatory Activity Of Questinol Isolated From Marine-Derived Fungus Eurotium amstelodami In Lipopolysaccharide-Stimulated RAW 264.7 Macrophages. Journal of Microbiology and Biotechnology, 24(10), 1346-1353. DOI: 10.4014/jmb.1405.05

Author Biographies

Wahyu Aji Mahardhika, Diponegoro University

Author Origin : Indonesia

Romario Dion, Universitas Diponegoro

Author Origin : Indonesia

Mochammad Fa’iq Qoys Naufal, Universitas Diponegoro

Author Origin : Indonesia

Warih Ramadhany, Universitas Diponegoro

Author Origin : Indonesia

Arina Tri Lunggani, Universitas Diponegoro

Author Origin : Indonesia

Downloads

Download data is not yet available.

How to Cite

Mahardhika, W. A., Dion, R. ., Naufal, M. F. Q., Ramadhany, W. ., & Lunggani, A. T. . (2022). Isolation and Characterization of Mold on Furniture in Biological Laboratory Environment Using Contact Plate Method. Jurnal Biologi Tropis, 22(3), 765–772. https://doi.org/10.29303/jbt.v22i3.3416

Most read articles by the same author(s)

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.