Phytochemical Testing and In Vitro Anti-inflammatory Activity on Ethanol Extract of Akar Kuning (Arcangelisia flava L) Stems from West Kalimantan
DOI:
10.29303/jbt.v22i4.4431Published:
2022-12-09Downloads
Abstract
The akar kuning plant (Arcangelisia flava L) from the Menispermaceae family is one of the Bajakah plant groups, which propagate among large trees and are commonly found in tropical forests in the West Kalimantan region. The purpose of this study was to determine the phytochemical compound and activity of anti-inflammatory from the akar kuning stem ethanol extract through the stabilization ability of the human red blood cell membrane (HRBC) in vitro. The akar kuning plant was taken from the forest of the Kapuas Hulu region, dried and mashed first and then extracted by maceration using the organic solvent ethanol. The ethanol extract obtained was concentrated using a rotary evaporatory, then a sample solution of akar kuning ethanol extract was made at several concentrations. Based on the results of phytochemical testing, this plant contained flavonoids, tannins, alkaloids and saponins. Anti-inflammatory activity testing using rabbit blood and using diclofenac sodium as a positive control. The measurement results showed the anti-inflammatory activity of the ethanol extract of the yellow root plant at concentrations of 50, 100, 150, 200 and 250 mg/mL were 28.77, 39.45, 57.53, , 62.80 and 75.50%, while the percentage of HRBC stabilization of the positive control was at the same concentration were 34.55, 46.34., 56.56, 69.88 and 87.33%.
Keywords:
anti-inflammatory; Arcangelisia flava L; HRBC; in vitroReferences
Adryan, F., Wahyuni, Malik F., Fariane, N., Ilyas M.Y., & Bafadal, M., (2020). Sahidin, F. In Vitro Antiinflammatory Activity of Etlingera elatior (Jack) R.M. Smith by Hrbc Membrane Stabilization Method. Jurnal Ilmu Kefarmasian Indonesia 18(2):150-157. DOI:
Armadany, F. I., Wahyuni, Ardianti, A. N. & Mallarangeng, T. A. (2019). Majalah Farmasetika, 4 (1), 144-151. DOI: https://doi.org/10.24198/mfarmasetika.v4i0.25873
Barnes, J., Anderson, L. A. & Philipson, J. D. (1996). Herbal Medicine, 2nd edition, Pharmacetical Press, London. P 313.
Caleja, C., Barros, L., Antonio, A. L., Oliveira, M. & Ferreira, I. C. F. R. (2017). A comparative study between natural and synthetic antioxidants: Evaluation of their performance after incorporation into biscuits. Food Chemistry, 216: 342-346. DOI: https://doi.org/10.1016/j.foodchem.2016.08.075
Damiti, S.A., Ysrafil, Abidin, Z., Rahmawati, Kamba, V., Hartati, Ishak, P., & Yusuf, G.Z.P. (2021). Uji Aktivitas Antiinflamasi Ekstrak Metanol Daun Tembelekan (Lanatana camara Linn.) Secara In Vitro Menggunakan Metode Stabilitasi Membran Sel Darah Merah. Journal of Experimental and Clinical Pharmacy, 1(1): 47-55
DOI: 10.52365/jecp.v1i1.202
https://doi.org/10.14710/ijfst.17.4.247-253
https://doi.org/10.35814/jifi.v18i2.731
Ihsan, H., Pratama, I. S., & Hanifa, N. I. (2021). Aktivitas Antiinflamasi Infusa Bunga Pukul Empat (Mirabilis jalapa L.) Secara In Vitro. Acta Pharmaciae Indonesia: Acta Pharm Indo, 9(1): 21-30. DOI: https://doi.org/10.20884/1.api.2021.9.1.3683
Jung, H. A., Jin, S. E., Ahn, B. R., Lee, C. M., & Choi, J. S. (2013). Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPS-stimulated RAW264. 7 macrophages. Food and chemical toxicology, 59, 199-206. DOI: 10.1016/j.fct.2013.05.061.
Kurnia, D., Prisdayanti, N., Marliani, L., Idar, & Nurochman, Z., (2019). Antiinflammatory Activity from Marine Microalgae Chlorella vulgaris Extract Used Human Red Blood Cells Stability Method (HRBC). Jurnal Kartika Kimia, 2 (2): 57-62. DOI: https://doi.org/10.26874/jkk.v2i2.34
Maryani & Happy Mursam. (2013). The Phytochemistry and The Anti-Bacterial Activity of Yellow Root (Arcangelisia flava Merr.) against Aeromonas hydrophila. Journal of Biology and Life Science ISSN 2157-6076 2013, 4 (2): 182-189. DOI: 10.5296/jbls.v4i2.3683.
Murningsih, T. & Ahmad, F. (2016). Evaluasi Aktivitas Anti-Inflamasi dan Antioksidan Secara In-Vitro, Kandungan Fenolat dan Flavonoid Total Pada Terminalia spp., Berita Biologi, 15 (2); 159-166. DOI: 10.14203/beritabiologi.v15i2.2264.
Pradina, U., Sapar, A., Warsidah, W., Sayekti, E., & Aritonang, A. B. (2022). Identifikasi Komponen Senyawa Organik dan Uji Aktivitas Antiinflamasi dari Fraksi Etil Asetat Daun Cabai Rawit (Capsicum frutescens L.). Al-Kimia, 10(1). DOI: https://doi.org/10.24252/al-kimia.v10i1.20456
Setyani, W., Setyowati, H., Palupi, D. H. S., Rahayunnissa, H. & Hariono, M. (2019). Antihyperlipidemia and Antihyperglycemic Studies of Arcangelisia flava (L.) Merr. Phenolic Compound: Incorporation of In Vivo and In Silico Study at Molecular Level. Indonesian Journal of Pharmaceutical Science and Technology Journal, 6 (2): 84-94. DOI: https://doi.org/10.24198/ijpst.v6i2.20211
Simonaro, C. M. (2016). Lysosomes, lysosomal storage diseases, and inflammation. Journal
of Inborn Errors of Metabolism and Screening, 4:1-8.
https://doi.org/10.1177/2326409816650465
Sofiana, M.S.J., Safitri, I., Warsidah, Helena, S., & Nurdiansyah, S.I., (2021). Antioxidant and Antiinflammatory Activities from Ethanol Extract of Eucheuma cottonii from Lemukutan Island Waters West Kalimantan Saintek Perikanan: Indonesian Journal of Fisheries Science and Technology, 17 (4): 247-253.
Suratno, Rizki, M. & Pratama, M. R. F. (2019). In-Vitro Study Of Antioxidant Activities From Ethanol Extracts Of Akar Kuning (Arcangelisia Flava), Jurnal Surya Medika, 4 (2). DOI: https://doi.org/10.33084/jsm.v4i2.594
Wahyudi, L. D., Ratnadewi, A. A. I. & Siswo, T. A. (2016). Potential Antioxidant and Antidiabetic Activities of Kayu Kuning (Arcangelisia flava). Agriculture and Agricultural Science Procedia, 9: 396-402. DOI: https://doi.org/10.1016/j.aaspro.2016.02.155Get rights and content
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