The Comparison of Analytical Methods for Determination of Eugenol Content in Water Soluble Base from Clove Flower (Syzygium aromaticum) Essential Oil Ointment using UV-Vis Spectrophotometry and High Performance Liquid Chromatography
Authors
Puput Herawati Said Hasan , Nining Sugihartini , Hari SusantiDOI:
10.29303/jbt.v23i2.4522Published:
2023-03-23Issue:
Vol. 23 No. 2 (2023): April-JuneKeywords:
eugenol, HPLC, method validation, UV spectrophotometry.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
The eugenol content in clove essential oil can be used as a parameter to determine the chemical stability of clove essential oil ointment. Both UV- Vis and HPLC spectrophotometry methods can be used to determine eugenol levels. However, no studies have compared the two methods. This study aims to find out which analytical method is more valid in determining the eugenol content of clove flower essential oil (Syzygium aromaticum) in water-sea based ointments, between the UV-Vis spectrophotometric method and the HPLC method. The parameters in the validation of the analytical method carried out include parameters of linearity, selectivity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The results of method validation using UV spectrophotometry showed linearity (R = 0.9998), precision (CV = 0.137%), LOD 0.190 µg/mL, LOQ 0.632µg/mL, accuracy (recovery = 100.05%). Method validation using HPLC produced parameters of linearity (R= 0.998), precision (CV = 0.533%), LOD 0.28 µg/mL, LOQ 0.93 µg/mL, accuracy (recovery = 106.43. Average levels of eugenol in water-soluble base MABC ointment using UV spectrophotometry (% concentration = 2.201 ± 0.037) while using HPLC (% concentration = 3.191 ± 0.023). Both methods met the validation requirements. The assay results using both methods met the requirements according to the Indonesian Pharmacopoeia Edition VI. The two methods did not have a significant difference based on the student test, both in the validation parameters and the results of determining the levels of eugenol.
References
Anief, M. (1997). Ilmu Meracik Obat. GajahMada University Press, Yogyakarta.
AOAC. (2011). Annex I: Validation Study Protocol
Astuti, R Harini, Dwi., & Wijayanti. (2012). Aplikasi Metode Spektrofotometri Visibel Untuk Mengukur Kadar Kurkuminoid Pada Rimpang Kunyit (Curcuma domestica). Prosiding Seminar Nasional Aplikasi Sains & Teknologi. Universitas Sanata Dharma. Yogyakarta (SNAST) Periode III.
Depkes RI. (1995). Farmakope Indonesia, Edisi IV, Departemen Kesehatan Republik Indonesia, Jakarta, 4, 223, 1009, 1061.
Devineni, J. (2017) New sensitive UV spectrophotometric method for simultaneous estimation of lopinavir and ritonavir in fixed dose combination as soft gels. International Journal of Pharmaceutical Development & Technology, 7 (1), 25-30.
Dewi, D. R. N., Zakkia, L. U., Khoiruddin, W., & Harismah, K. (2018). Pengaruh pH Terhadap Lamanya Penyimpanan Sediaan Esktrak Daun Seligi Dan Eugenol Dari Minyak Daun Cengkeh Sebagai Obat Antinyeri. Prosiding SNST Fakultas Teknik, 1(1)
Dewi, D. R. N., Zakkia, L. U., Khoiruddin, W., & Harismah, K. (2018). Pengaruh pH Terhadap Lamanya Penyimpanan Sediaan Esktrak Daun Seligi Dan Eugenol Dari Minyak Daun Cengkeh Sebagai Obat Antinyeri. Prosiding SNST Fakultas Teknik, 1(1).
Kementerian Kesehatan RI. (2020). Farmakope Indonesia edisi VI. Kementerian Kesehatan Republik Indonesia.
Miller, J.C. and Miller, J.N. (1988). Statistics for Analytical Chemistry, 2 ndEdition, John Wiley & Sons, New York, 109-120.
Miller, J.C. dan J.N. Miller. (1991). Statistika Untuk Kimia Analisis. Diterjemahkan oleh Suroso, ITB, Bandung.
Mustariani, B. A. A., Rahmawati, S., & Aulia, N. H. (2021). Potensi Salep dari Fraksi Aktif Bawang Merah Bima (Allium Sp) Sebagai Penghambat Infeksi Sekunder Jamur Patogen Penyebab Luka Diabetes. Jurnal Kesehatan Bakti Tunas Husada, Jurnal Ilmu-Ilmu Keperawatan, Analis Kesehatan Dan Farmasi, 21(2), 194–206.
Pratimasari, D., Sugihartini, N., & Yuwono, T. (2015). Evaluasi Sifat Fisik Dan Uji Iritasi Sediaan Salep Minyak Atsiri Bunga Cengkeh Dalam Basis Larut Air. Jurnal Ilmiah Farmasi, 11(1), 9–15. https://doi.org/10.20885/jif.vol11.iss1.art2
Putra, M. M., Dewantar, I., & Swastini, D. A. (2017). Pengaruh lama penyimpanan terhadap nilai pH sediaan cold cream kombinasi ekstrak kulit buah manggis (Garcinia
Putra, M. M., Dewantar, I., & Swastini, D. A. (2017). Pengaruh lama penyimpanan terhadap nilai pH sediaan cold cream kombinasi ekstrak kulit buah manggis (Garcinia Jacob et al Jurnal Kajian Veteriner 49 mangostana L.), herba pegagan (Centella asiatica) dan daun gaharu (Gyrinops versteegii (gilg) Domke). Jurnal Farmasi Udayana, 3(1), 279745.
Rohman, A. (2009). Kromatografi Untuk Analisis Obat, Edisi pertama, Cetakan Pertama, Graha Ilmu, Yogyakarta, 111.
Salunke, J.M., Pawar, D.S., Chavhan, V.D., and Ghante, M.R. (2013). Simultaneous UV spectrophotometric method for estimation of ritonavir and lopinavir in bulk and tablet dosage form, Der Pharmacia Lettre,5 (3), 156–162.
Sari, A., & Maulidya, A. (2015). Formulasi Sediaan Salep Ekstrak Etanol Rimpang Kunyit (Curcuma longa Linn). Sel Jurnal Penelitian Kesehatan, 3(1), 16–23.
Snyder, R.L., Kirkland, J.J., and Glajch, J.L., (1997). Practical HPLC Method Developement, 2 ndEdition, John Wiley & Son, Inc., New York, 686-697
Sugihartini, N., Mugita Sari, D. E., Bachri, M. S., & Yuliani, S. (2019). The Amount of βCarotene, Total Phenolic and Total Flavonoid of Ethanol Extract of Leaf Moringa Oleifera with Variation Concentration of Solvent. 33–37. https://doi.org/10.2991/adics-phs-19.2019.22
Susilowati, E., & Saptuti W, S. (2014). Optimasi Sediaan Salep Yang Mengandung Eugenol Dari Isolasi Minyak Cengkeh (Eugenia caryophylatta Thunb.). Indonesian Journal on Medical Science, 1(2), 29–34.
Wenzel, T. 2021. Molecular and Atomic Spectroscopy. LibreTexts. doi: 10.1021/ed010p308.
Zulkarnain, A. K., Susanti, M., & Lathifa, A. N. (2013). Stabilitas fisik sediaan lotion o/w dan w/o ekstrak buah mahkota dewa sebagai tabir surya dan uji iritasi primer pada kelinci. Traditional Medicine Journal, 18(3), 141-150.
License
Copyright (c) 2023 Puput Herawati Said Hasan, Nining Sugihartini, Hari Susanti
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.