Compatibility Test Consorcium of Thermophilic Bacteria Producing Xylanase Enzym from The Hot Water of Mudiak Sapan (MS18, MSS15, MSS11, MS16)
DOI:
10.29303/jbt.v23i2.4761Published:
2023-03-17Downloads
Abstract
Mudiak Sapan hot spring, South Solok Regency, West Sumatra is one of the hot springs where there are various species of thermophilic bacteria that produce xylanase enzymes. The xylanase enzyme is a group of enzymes that has the ability to hydrolyze xylan (hemicellulose) or polymers into xylose and xylo-oligosaccharides. The purpose of this study was to test the compatibility of bacterial isolates to become a xylanase-producing bacterial consortium. This research is a descriptive research that informs the compatibility of thermophilic bacterial isolates from the Mudiak Sapan hot springs. The method used in this study was disk diffusion, based on the research, it was found that 5 combinations of compatible xylanase enzyme-producing isolates were MS18 x MSS11; MS18 x MSS15; MSS18 x MS16 ; MSS11 x MS16 ; MSS15 x MS16 and 1 combination of incompatible isolates namely MSS11 x MSS15 which had an inhibition zone of 0.645 cm.
Keywords:
compatibility, thermophilic bacteria, xylanase enzyme.References
Asri AC & Zulaika E. (2016). Sinergisme Antar Isolat Azotobacter yang Dikonsorsiumkan. Jurnal Sains dan Seni ITS, 5 (2): 2337-3520. DOI: http://dx.doi.org/10.12962/j23373520.v5i2.20693
Edy N. (2011). Pengendalian Hayati Penyakit Darah Pada Pisang dengan Pseudomonad Fluoresen dan Bacillus spp. Agroland. 18(1):29-35. URL: http://jurnal.untad.ac.id/jurnal/index.php/AGROLAND/article/view/2502
Irdawati and M, Fifendy. (2011). Isolation Bacteria Thermophilic Producer Amylase from Hot Springs Rimbo House Pasaman. Report Research. University Country of Padang. http://repository.unp.ac.id/
Irdawati, S., Agustien, A., & Rilda, Y. (2016). Xylanase enzyme stability and biochemical characteristics thermoxylanolytic bacteria from mudiak sapan hot springs at Solok Selatan district. Der Pharm Lett, 8, 254-261. URL: https://www.scholarsresearchlibrary.com/
Irdawati, Syamsuardi, Agustien., A., Rilda,Y., Alberida, H. (2017). Profil Pertumbuhan Bakteri Termofilik Penghasil Xylanase Alkali Dari Sumber Air Panas Mudiak Sapan, Solok Selatan. Prosiding Seminar Nasional Pendidikan dan Sains Biologi 2. ISBN: 978-602-742242-1 URL: http://repository.unp.ac.id/27774/
Jiang, Y., Lv, Y., Wu, R., Lu, J., Dong, W., Zhou, J., & Jiang, M. (2020). Consolidated bioprocessing performance of a two‐species microbial consortium for butanol production from lignocellulosic biomass. Biotechnology and Bioengineering, 117(10), 2985-2995. DOI: https://doi.org/10.1002/bit.27464
Jovanita, L., & Advinda, L. (2022). Compatibility Test of Fluorescent Pseudomonad Isolated from Plant Rhizosphere. Jurnal Serambi Biologi, 7(1), 65-69. URL: https://serambibiologi.ppj.unp.ac.id/index.php/srmb/article/view/21
Komarawidjaja, W. (2009). Karakteristik dan Pertumbuhan Konsorsium Mikroba Lokal dalam Media Mengandung Minyak Bumi. Jakarta. Jurnal Teknik Lingkungan, 10 (1) : 114 – 119. DOI: https://doi.org/10.29122/jtl.v10i1.1510
Li S., X. Yang, S. Yang, M. Zhu, and X. Wang. (2012). Technology Prospecting on Enzymes: Application, Marketing and Engineering. Computational and Structural Biotechnology Journal. Vol 1, No. 2. DOI: https://doi.org/10.5936/csbj.201209017
Lima, M. A., Oliveira-Neto, M., Kadowaki, M. A. S., Rosseto, F. R., Prates, E. T., Squina, F. M. & Polikarpov, I. (2013). Aspergillus niger β-glucosidase has a cellulase-like tadpole molecular shape: insights into glycoside hydrolase family 3 (GH3) β-glucosidase structure and function. Journal of Biological Chemistry, 288(46), 32991-33005. DOI: https://doi.org/10.1074/jbc.M113.479279
Mardawati, E., Daulay, D. N., Wira, D. W., & Sukarminah, E. (2018). Pengaruh Konsentrasi Sel Awal dan pH Medium pada Fermentasi Xilitol dari Hidrolisat Tandan Kosong Kelapa Sawit. Industria: Jurnal Teknologi dan Manajemen Agroindustri. Vol 7(1), 23-30. DOI: https://doi.org/10.21776/ub.industria.2018.007.01.3
Melim Miguel, A. S., Martins-Meyer, T. S., da Costa Figueiredo, E. V., Paulo-Lobo, P. W., & Dellamora-Ortiz, G. M. (2013). Enzymes in bakery: current and future trends. Food industry. DOI: https://doi.org/10.5772/53168
Ningsih, D. R., Rastuti, U., & Kamaludin, R. Karakterisasi enzim amilase dari bakteri Bacillus amyloliquefaciens. Prosiding, 3(1). URL: http://www.jurnal.lppm.unsoed.ac.id/ojs/index.php/Prosiding/article/download/226/225
Pratita, M. Y. E., dan Surya, R. P. (2012). Isolasi dan Identifikasi Bakteri Termofilik dari Sumber Mata Air Panas di Songgoriti Setelah Dua Hari Inkubasi. Jurnal Teknik Pomits, 1(1): 1-5. URL: https://www.academia.edu/35415264/Isolasi_dan_identifikasi_bakteri_termofilik_dari_sumber_mata_air_panas_di_Songgoriti_setelah_dua_hari_inkubasi
Wahyudi, P., Suwahyono, U., & Mulyati, S. (2010). Pertumbuhan Trichoderma harzianum pada Medium yang Mengandung Xilan. Jurnal Ilmu kefarmasian, 1-7. URL: https://www.academia.edu/download/32423941/Priyo-w..Pertumbuhan-Trichoderma.pdf
Sarkar, P. and Chourasia R. (2017). Bioconversion of organic solid wastes into biofortified compost using a microbial consortium. International Journal of Recycling of Organic Waste in Agriculture, 6, pp. 321–334. DOI: https://doi.org/10.1007/s40093-017-0180-8
Siregar, M., & Prayitno, S. B. (2016). Pengaruh Konsentrasi Konsorsium Bakteri K1, K2 Dan K3 Terhadap Status Kesehatan Rumput Laut (Eucheuma Cottonii). Journal of Aquaculture Management and Technology, 5(1), 91-97. URL: https://ejournal3.undip.ac.id/index.php/jamt/article/view/10699
Susilowati, P. E., Raharjo, S., Kurniawati, D., Rahim, R., Sumarlin, A., & Ardiansyah, A. 2012. Produksi Xilanase dari Isolat Sumber Air Panas Sonai, Sulawesi Tenggara, menggunakan Limbah Pertanian. Jurnal Natur Indonesia, 14(3), 1-6. DOI: https://doi.org/10.31258/jnat.14.3.199-204
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