Propagation of Gaharu Plant Gyrinops Versteegii Species Provenant Beringin Throught in Vitro Culture
DOI:
10.29303/jbt.v23i2.4796Published:
2023-03-08Downloads
Abstract
Grynops verstegii is a non-timber forest product commodity that has a very high economic value. People know this tree as the agarwood tree. In Lombok, the Beringin provenance is one of the leading provenances, so it is very important to conserve its germplasm. This study aims to find alternatives for gaharu development through seed production using in vitro culture techniques. This research was carried out in July 2021 at the Tissue Culture Unit of the Immunobiology Laboratory, University of Mataram. In this experiment there were 8 media treatments with different concentrations of BAP (Ssitokini) and NAA (auxin). The results showed that callus growth occurred on MS 1, MS 3, MS 4, MS 6, and MS 7 media. However, the best growth was obtained in MS 1 treatment with a combination of 1.0 ppm BAP and 0.5 ppm NAA. While most of the samples experienced browning and contamination.
Keywords:
callus, culture, Grynops verstegii, provenance beringin.References
Ayuningrum, K., Budisantoso, I., & Kamsinah (2015). Pemberian Hormon 2,4-D dan BAP Terhadap Pertumbuhan Subkultur Kalus Kedelai (Glycine max (L.) Merrill) Secara In Vitro. Biosfera, 32(1): 59-65.
Azah, Ma. N., S. S. Husni, J Mailina, L Sahrim, J A. Majid & Z M. Faridz (2013). Classification of Agarwood (Gaharu) by Resin Content. Journal of Tropical Forest Science 25(2): 213–219.
Lestari, E. G, (2011). Peranan Zat Pengatur Tumbuh Dalam Perbanyakan Tanaman Melalui Kultur Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jurnal Agrobiogen, Bogor, 7(1) : 63-68.
Mega, D.A. & Swastini (2012). Screening phytochemical and free antiradical activity of methanol leaf extract of gaharu (Gyrinops versteegii). Journal of Chemistry 4 (2): 187-192.
Millang S., Bachtiar B., & Makmur A. (2011). Awal pertumbuhan pohon gaharu (Gyrinops sp) asal Nusa Tenggara Barat di hutan pendidikan Universitas Hasanudin. Hutan dan Masyarakat 6(2): 117-124.
Mohamed, R. & Shiou Lee. (2017). Keeping Up Appearances: Agarwood Grades and Quality. Chapter 10. https://www.researchgate.net/publication/3037 29688.
Mulyaningsih T, Muspiah A, & Aryanti E. (2018). Keanekaragaman morfologi Gyrinops versteegii (Thymelaceae) di NTB. Laporan Penelitian Universitas Mataram, Mataram.
Mulyaningsih, T., D. Marsono, Sumardi & I. Yamada (2014). Selection of Superior Breeding Infraspecies Gaharu of Gyrinops versteegii (Gilg) Domke, Journal of Agricultural Science and Technology B, 4: 485-92.
Mulyaningsih, T., D. Marsono, Sumardi & I. Yamada (2015). Community of Eaglewood Gyrinops versteegii (Gilg.) Domke. and the Diversity of Plant Species Associated in Western Lombok Forest. Paper of International Seminar on The Tropical Natural Resources 2015. Url: https://www. researchgate.net/publication/301301497, 2 Juni 2016.
Mulyaningsih, T., D. Marsono, Sumardi & I.Yamada (2017). Keragaman Infraspesifik Gaharu Gyrinops Versteegii (Gilg.) Domke di Pulau Lombok Bagian Barat. Jurnal Penelitian Hutan dan Konservasi Alam. 14(1): 1-10
Nadeak, Romasli, Nelly, A., & Edy B. (2015). Respon Eskplan Biji Gaharu (Aquilaria Malaccencis Lamk.) Terhadap Pemberian NAA dan IBA Secara In Vitro, Program Studi Kehutanan, Fakultas Pertanian, Universitas Sumatera Utara.
Patma, Utri. (2013). Respon Media Tanam Dan Pemberian Auksin Asam Asetat Naftalen Pada Pembibitan Aren (Arenga pinnata Merr), Jurnal Online Agroekoteknologi, 1(2).
Susilo, A., Kalima, T., & Santoso, E., (2014). Panduan Lapangan Pengenalan Jenis Pohon Penghasil Gaharu Gyrinops Spp, di Indonesia. Bogor, Indonesia: Kementerian Kehutanan, Badan Penelitian dan Pengembangan Kehutanan, Pusat Penelitian dan Pengembangan Konservasi dan Rehabilitasi - International Tropical Timber Organization (Itto).
Thomy, Z., (2012), Effect of Plant Growth Regulator 2,4-D and BAP on callus Growth of Plants Producing Gaharu, Prasiding Seminar Hasil Nasional Biologi, Medan.
Wahyuni A., Satria B., & Zainal A. (2020). Induksi kalus gaharu dengan NAA dan BAP secara in vitro. Agrosains: Jurnal Penelitian Agronomi 22 (1): 39-4
Widyastuti, N., & Deviyanti, J., (2018). Kultur Jaringan Teori dan Praktik Perbanyakan Tanaman Secara In Vitro, penerbit ANDI, Yogyakarta.
License
Copyright (c) 2023 Aida Muspiah, Tri Mulyaningsih, Eka Sunarwidhi Prasedya

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.























