Content of Chlorophyll, Antioxidants, and Metabolite Compounds in the Leaf Development Stage of Murraya koenigii (L.) Spreng
DOI:
10.29303/jbt.v23i3.4869Published:
2023-06-12Downloads
Abstract
Murraya koenigii (L.) Spreng is often known as Temurui or Curry as a local name in Indonesia, and has long been used in medicine and has as a potential as a multi-medicinal plant. Temurui as a producer of bioactivity has the antioxidant properties. This is due to the presence of metabolite compounds contained in the leaves of the Temurui plant. Leaves are organs that are always growing where chemical components such as chlorophyll in the leaves will also develop thereby affecting leaf metabolism in plants. This study aims to determine the contens of chlorophyll, contens of antioxidants and metabolite compounds in Temurui leaves at the level of leaf development. Different leaf extractions are also used to measure the antioxidant, chlorophyll content and metabolite compounds found in Temurui leaves. The research results obtained on young, and old Temurui leaves obtained a total of 3 groups of dominant compounds, terpenoids, organic acids and esters, and mature leaves not find ester compound. The level of leaf development affects the contents of antioxidants and chlorophyll contents. Chlorophyll content in mature leaves was 44.60ug/ml, young leaves were 41.28ug/ml and old leaves were 30.27ug/ml while antioxidant contents in young leaves were 8.949ppm, mature leaves were 8.85ppm and old leaves were 8.429ppm.
Keywords:
: antioxidant, chlorophyll, Murraya koenigii (L.) spreng, metabolite.References
Ao, K. (2019). Investigation of Antioxidant Activity (In Vitro) and Gas Chromatography-Mass Spectrometry Profiling of Portucala oleracea L. And Portulaca grandiflora Hook. Extracts. Asian Journal of Pharmaceutical and Clinical Research. 12(3), 348-352. DOI: 10.22159/ajpcr.2019.v12i3.30621.
Badarinath, A., Rao, K., Chetty, C. S., Ramkanth, S., Rajan, T., & Gnanaprakash K. (2010). A Review on In-vitro Antioxidant Methods: Comparisons, Correlations, and Considerations. International Journal of PharmTech Research. 2(2), 1276-1285.
Chauhan, B., Dedania, J., & Mashru, R. C. (2017). Review on Murraya koenigii: versatile role in management of human health. World Journal of Pharmacy And Pharmaceutical Sciences. 6(3), 476-493. DOI: 10.20959/wjpps20172-8740.
Dharmadewi, A.A.I.M (2020). Analisis Kandungan Klorofil pada Beberapa Jenis Sayuran Hijau sebagai Alternatif Bahan Dasar Food Suplement. Jurnal Edukasi Matematika dan Sains. 9(2), 171-176. Doi: 10.5281/zenodo.4299383.
Dunn, W.B., Bailey, N.J.C. & Johnson, H.E., (2005). Measuring the Metabolome: Current Analytical Technologies. Analyst. 5(130), 606-625. DOI: doi.org/10.1039/B418288J.
Eko. (2007). Budidaya Tanaman Sayuran Sawi Pakcoy. Jakarta: Penerbit Swadaya.
Fiehn, O., Kopka, J., Dormann, P., Altmann, T., Trethewey, R.N., & Willmitzer, L., (2000), Metabolite profiling for plant functional genomics, Nature Biotechnology. 18(11), 1157-1161. DOI: 10.1038/84453.
Gomis, D. B. (2000). HPLC analysis of organic acids. dalam : Nollet, L. M. L. (ed). Food Analysis by HPLC. CRC Press, New York. Pp. 477-492
Julianto, T.S. (2019). Fitokimia Tinjauan Metabolit Sekunder dan Skrining Fitokima.Yogyakarta: Universitas Islam Indonesia, Hal. 35-40.
Khafid., A., Suedy, S.W.A., & Nurchayati, Y. (2021). Kandungan Klorofil dan Karotenoid Daun Salam (Syzigium polyanthum (Wight) Walp.) pada Umur yang Berbeda. Buletin Anatomi dan Fisiologi. 6(1), 74-80. DOI: 10.14710/baf.6.1.2021.74-80.
Mandal, S., Yadav, S., & Nema, R.K. (2009). Antioxidants: A Review. Journal of Chemical and Pharmaceutical Research. 1(1), 102-104.
Molyneux, P. (2004). The Use of The Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity, Songklanakarin. J. Sci. Technol. 26(2), 211-219. http://rdo.psu.ac.th/sjstweb/index.php
Pujaningsih R.I., Sulistyanto B., & Sumarsih S. (2018). Observation of Muntingia calabura’sLeaf Extract as Feed Additive for Livestock Diet. IOP Conference Series: Earth and Enviromental Science, p119.
Soerawidjaja & Tatang H. (2005), Minyak-lemak dan produk-produk kimia lain dari kelapa, Handout kuliah Proses Industri Kimia, Program StudiTeknik Kimia, Institut Teknologi Bandung, Bandung.
Setiawati, T., Saragih, I. A., Nurzaman, M. & Mutaqin, A. Z. (2016). Analisis Kadar Klorofil dan Luas Daun Lampeni (Ardisia humilis Thunbergh) Pada Tingkat Perkembangan Yang Berbeda di Cagar Alam Pengandaran. Prosiding Seminar MIPA Peran Penelitian Ilmu Dasar dalam Menunjang Pembangunan Berkelanjutan. Universitas Padjajaran: Jatinangor.
Solikhah, R., Purwantoyo, E dan Rudyatmi, E. (2019). Aktivitas Antioksidan Dan Kadar Klorofil Kultivar Singkong Di Daerah Wonosobo. Life Science. 8(1), 86-95. doi.org/10.15294/lifesci.v8i1.30001.
Sukma, F. F., Sahara, D., Ihsan, F. N., Halimatussakdiah, Wahyuningsih, P., & Amna, U. (2018). Skrining fitokimia ekstrak daun “temurui” (Murraya koenigii (L.) Spreng) Kota Langsa, Aceh. Jurnal Jeumpa. 5(1), 34-39.
Sumenda, L. (2011). Analisis Kandungan Klorofil Daun Mangga (Mangifera indica L.) pada Tingkat Perkembangan Daun yang Berbeda. Jurnal Bios Logos. 1(1): 20-24. DOI: doi.org/10.35799/jbl.1.1.2011.372.
Theron, M.M., & Lues, J.F.R. (2010). Organic Acids and Food Preservation (1st ed.). CRC Press.
Tristantini, D., Ismawati, A., Pradana, B. T., & Jonathan, J. G. (2016). Pengujian aktivitas antioksidan menggunakan metode DPPH pada daun tanjung (Mimusops elengi L). In Seminar Nasional Teknik Kimia Kejuangan (p. 1).
Xiao, Q., Mu, X., Liu, J., Li B., Liu H., Zhang B., & Xiaoet, P. (2022). Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials. Chin Med 17(45). DOI: doi.org/10.1186/s13020-022-00601-y
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