Identification of Escherichia coli Sub Type Enterotoxigenic (ETEC) from Food Samples Using Pcr (Polymerase Chain Reaction) Technique
DOI:
10.29303/jbt.v23i4.5540Published:
2023-10-04Downloads
Abstract
Escherichia coli is a normal flora of the digestive tract of humans and animals and is important in the digestion of food. This bacteria lives in feces, can cause health problems, such as diarrhea and vomiting. This research was conducted for consideration in the prevention of diarrhea, processing, marketing of food. This study aims to determine the presence of enterotoxigenic Escherichia coli sub-type (ETEC) from food samples. This research is an explorative descriptive study, as many as 5 samples from food, food samples taken are food consumed every day and some include traditional food in Mataram City. The samples were cultured on BHI-Broth media, followed by inoculation on Eosin Methylene Blue (EMB) medium and continued with the Gram stain test. Colonies that are metallic green in colour and show the characteristics of Escherichia coli bacteria on Gram staining are then carried out for purification of the bacteria on Nutrient Agar (NA) medium, if the classification of bacteria shows the characteristics of Escherichia coli bacteria on Gram staining then proceed with the Indole/sugar-test. The results showed that of the 5 food samples isolated, only 1 sample showed positive E.coli bacteria. Then proceed to the PCR (Polymerase Chain Reaction) stage using the qualitative PCR method to determine the presence of Enterotoxigenic Escherichia coli sub-type (ETEC) bacteria in food samples. , with a target gene of 237 bp.
Keywords:
Escherichia coli, food, Polymerase Chain Reaction (PCR).References
Amalia, W. (2017). Analisis Sistem Pengelenggaraan Makanan Dan Hubungan Asupan Energi Dan Zat Gizi Makro Dengan Status Gizi Pada Santri Di Pondok Pesantren Daarul Rahman. Skripsi Program Studi Ilmu Gizi Fakultas Ilmu-Ilmu Kesehatan Universitas Esa Unggul.
Anisa, Y.,Y. Hadiatun, N. Hijriani, B., I. (2023). Gambaran Bakteri Eschericia coli pada Cincau Hitam dengan Metode MPN di Pasar Kota Malang. Jurnal Sains Natural, 1(2), 36-40. DOI: https://doi.org/10.35746/jsn.v1i2.343
Depkes. (2003). Tentang Pedoman Persyaratan Hygiene Sanitasi Makanan Jajanan.
Dikes Kota Mataram. (2009). Penderita Penyakit Diare Dikota Mataram Meningkat. ANTARA NTB
Gitaswari, D. A. I., & Budayanti, S. (2019). Identifikasi Subtipe Enterotoxigenic Escherichia coli Dan Enteroaggregative Escherichia coli Dari Spesimen Usap Dubur Penjamah Makanan Di Denpasar Menggunakan Polymerase Chain Reaction. E-Jurnal Medika Udayana, 8(1), 7. DOI: https://doi.org/10.24922/eum.v8i1.45223
Nuryani, D., Putra, N, Y., & Sudana, I, B. (2016). Kontaminasi Escherichia coli pada Makanan Jajanan di Kantin Sekolah Dasar Negeri Wilayah Denpasar Selatan. Ecotrophic, 10 (1), 28-32. URL: https://ojs.unud.ac.id/index.php/ECOTROPHIC/article/download/21518/14219/
Radji, M. (2011). Deteksi Cepat Bakteri Escerichia Coli Dalam Sampel Air Dengan Metode Polymerase Chain Reaction Menggunakan Primer 16E1 Dan 16E2. Makara, Sains, 14(1), 39–43. URL: http://journal.ui.ac.id/index.php/science/article/view/474/470
Rostini, T., & Rita, C. (2018). Elektroforesis Protein Serum Pasien Dengan Kadar Protein Normal. Indonesian Journal of Clinical Pathology and Medical Laboratory, 15(3), 87. DOI: https://doi.org/10.24293/ijcpml.v15i3.962
Dwiari, S, R., Asadayanti, D, D., Nurhayati., Sofyaningsih, M., Yudhanti, S, F., Yoga, I, B, K, W (2008). Teknologi Pangan Jilid 1. Direktorat Pembinaan SMK. Depdiknas
Soemarno. (2000). Identifikasi Bakteri Klinik. Akademi Analis Kesehatan Yogyakarta. Yogyakarta
Santoso T, J., Hidayat., Herman., dan Sudarsono (2013). Aplikasi Teknik Polymerase Chain Reaction (PCR) Menggunakan Primer Degenerate dan Spesifik Gen AV1 Untuk Mendeteksi Begomovirus Pada Tomat (Lycopersicon esculentum Mill.). J. Hort. Indonesia 4(3):140-149. URL: https://core.ac.uk/download/pdf/295173217.pdf
Takahasi Ichiro., Marinaro., Hiroshi Kiyono., Raymond J., Jackson., Nakagawa., Fujihashi., Hamada., John D. Clements., Kenneth L., Bost and Jerry R. McGhee., (1996). Mechanisms for Mucosal Immunogenicity and Adjuvancy of Escherichia coli Labile Enterotoxin. The Journal of Infectious Diseases. 173 (3). 627-635. URL: https://www.jstor.org/stable/30126182
Titiek. (2007). Cemaran Mikroba Pada Produk Pertanian, Penyakit Yang Ditimbulkan dan Pencegahannya. Jurnal Litbang Pertanian, 26(2).
Trisno, K., Tono, K. P., & Gusti Ketut Suarjana, I. (2019). Isolasi dan Indentifikasi Bakteri Escherichia Coli dari Udara pada Rumah Potong Unggas Swasta di Kota Denpasar. Indonesia Medicus Veterinus, 8(5), 2477–6637. DOI: https://doi.org/10.19087/imv.2019.8.5.685
Tuhu, N. (2010). Kemajuan Tekhnologi Dan Pola Hidup Manusia Dalam Prespektif Social Budaya. Jurnal Perkembangan Pendidikan:Fondasi Dan Amplikasi, 2(1), 34–35.
Wardhana, D. K., Safitri, D. A., Annisa, S., Effendi, M. H., & Harijani, N. (2021). Detection of Escherichia coli Contamination using Most Probable Number (MPN) methods in Chicken Meats in Market of Surabaya. Jurnal Medik Veteriner, 4(1), 118. DOI: https://doi.org/10.20473/jmv.vol4.iss1.2021.118-124
Wheeler. (1990). Mikrobiologi Dasar Edisi Kelima Jilid 2. Erlangga.
Widyastuti dan Almira. (2019). Higiene Dan Sanitasi Dalam Penyelenggaraan Makanan. K-Media. Yogyakarta
License
Copyright (c) 2023 Deby Anggryani, Edy Kurniawan, Dhika Juliana Sukmana, Jumari Ustiawaty
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
