Callus Induction of Dendrobium discolor Through The Thin Cell Layer (TCL) Technique Added with 2,4-Dichlorophenoxyaceticacid
DOI:
10.29303/jbt.v23i4b.5808Published:
2023-11-02Downloads
Abstract
Thin Cell Layer (TCL) is a plant propagation technique using thin explants (1-2 mm) through tissue culture. Callus induction is a crucial step in establishing plant regeneration. The type and concentration of growth regulators influence callus formation. 2.4 D is a growth regulator that is commonly used for callus induction in various plants. The research aims to determine the effectiveness of thin explants and 2,4-D in inducing Dendrobium discolor callus. The method used a Completely Randomized Design (CRD) with four treatments (1, 2, 3, and 4 mg/L). Adding 1, 2, and 3 mg/L 2,4-D can induce 100% calluses through the TCL explant. The callus has a friable to compact texture. The result showed that 2 mg/L 2,4-D was the best concentration for callus induction of D. discolor through TCL technique, indicating the greenish color, friable texture, and meristematic tissue.
Keywords:
2,4-D, callus, Dendrobium discolor, TCL.References
Apriliyani, R., & Wahidah, B. F. (2021). Perbanyakan anggrek Dendrobium sp. secara in vitro: Faktor-faktor keberhasilannya. Filogeni: Jurnal Mahasiswa Biologi, 1(2). DOI: https://doi.org/10.24252/filogeni.v1i2.21992
Dalila, Z. D., Jaafar, H., & Manaf, A. A. (2013). Effects of 2,4-D and kinetin on callus induction of Barringtonia racemosa leaf and endosperm explants in different types of basal media. Asian Journal of Plant Sciences, 12(1). DOI: https://doi.org/10.3923/ajps.2013.21.27
Fandani, H. S., Mallomasang, S. N., & Korja, I. N. (2018). Keanekaragaman Jenis Anggrek pada beberapa Penangkaran di Desa Ampera dan Desa Karunia Kecamatan Palolo Kabupaten Sigi. Jurnal Warta Rimba, 6(9). URL: http://jurnal.untad.ac.id/jurnal/index.php/WartaRimba/article/view/11297
Fitroh, A. I., Dwiyani, R., Wijaya, I. K. A., & Yuswanti, H. (2018). Pengaruh 2,4-D terhadap Induksi Kalus Daun Stroberi (Fragaria sp.) dengan Media Alternatif Nutrisi Hidroponik AB Mix. E-Jurnal Agroekoteknologi Tropika, 7(3). URL: https://ojs.unud.ac.id/index.php/JAT/article/view/42172
Ibrahim et al. (2013). Induksi Kalus Embriogenik dan Daya Regenerasi Kopi Arabika Menggunakan 2,4- Dichlorophenoxyacetic Acid dan 6-Benzyladenine. Jurnal Tanaman Industri Dan Penyegar, 4(2).
A., Rymen, B., Lambolez, A., Kojima, M., Takebayashi, Y., Heyman, J., Watanabe, S., Seo, M., De Veylder, L., Sakakibara, H., & Sugimoto, K. (2017). Wounding triggers callus formation via dynamic hormonal and transcriptional changes. Plant Physiology, 175(3). DOI: https://doi.org/10.1104/pp.17.01035
Iqbal, A., Khan, R. S., Khan, M. A., Gul, K., Aizaz, M., Usman, M., & Arif, M. (2022). Efficient Regeneration in Sugarcane Using Thin Cell Layer (TCL) Culture System. Sugar Tech, May. DOI: https://doi.org/10.1007/s12355-022-01162-y
Ma, N. L., Khoo, S. C., Lee, J. X., Soon, C. F., & AB Shukor, N. A. (2020). Efficient micropropagation of Dendrobium aurantiacum from shoot explant. Plant Science Today, 7(3), 476–482. DOI: https://doi.org/10.14719/PST.2020.7.3.724
Mahadi, I., Syafi’i, W., & Sari, Y. (2016). Induksi Kalus Jeruk Kasturi (Citrus microcarpa) Menggunakan Hormon 2,4-D dan BAP dengan Metode in vitro. Jurnal Ilmu Pertanian Indonesia, 21(2). DOI: DOI: https://doi.org/10.18343/jipi.21.2.84
Murashige, T., & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15(3), 473–497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Parthibhan, S., Rao, M. V., Teixeira da Silva, J. A., & Senthil Kumar, T. (2018). Somatic embryogenesis from stem thin cell layers of Dendrobium aqueum. Biologia Plantarum, 62(3). DOI: https://doi.org/10.1007/s10535-018-0769-4
Purba, R. V., Yuswanti, H., & Astawa, I. N. G. (2017). E-Jurnal Agroekoteknologi Tropika Induksi Kalus Eksplan daun Tanaman Anggur (Vitis vinivera L.) dengan Aplikasi 2,4-D Secara in Vitro. E-Jurnal Agroekoteknologi Tropika, 6(2). URL: https://ojs.unud.ac.id/index.php/JAT/article/view/30895
Rachmawati, F., Permanik, D., Mayang, R. B., & Winarto, B. (2020). Protokol Perbanyakan Masal Dendrobium ‘Balithi CF22-58’ secara In Vitro Melalui Embriogenesis Somatik Tidak Langsung (In Vitro Propagation Protocol of Dendrobium ‘Balithi CF22-58’ via Indirect Somatic Embryogenesis). Jurnal Hortikultura, 29(2), 137. DOI: https://doi.org/10.21082/jhort.v29n2.2019.p137-146
Rasud, Y., & Bustaman. (2020). Induksi Kalus secara In Vitro dari Daun Cengkeh (Syizigium aromaticum L.) dalam Media dengan Berbagai Konsentrasi Auksin. Jurnal Ilmu Pertanian Indonesia, 25(1). DOI: https://doi.org/10.18343/jipi.25.1.67
Sharma, V., Magotra, T., Chourasia, A., Mittal, D., Singh, U. P., Sharma, S., Sharma, S., Ramírez, Y. G., Dobránszki, J., & Martinez-montero, M. E. (2023). Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species. June. DOI: https://doi.org/10.3390/f14061212
Teixeira Da Silva, J. A., & Dobránszki, J. (2019). Recent advances and novelties in the thin cell layer-based plant biotechnology - a mini-review. Biotechnologia, 100(1). DOI: https://doi.org/10.5114/bta.2019.83215
Vudala, S. M., Padial, A. A., & Ribas, L. L. F. (2019). Micropropagation of Hadrolaelia grandis through transverse and longitudinal thin cell layer culture. South African Journal of Botany, 121, 76–82. DOI: https://doi.org/10.1016/j.sajb.2018.07.017
Wattanapan, N., Nualsri, C., & Meesawat, U. (2018). In vitro propagation through transverse thin cell layer (Ttcl) culture system of lady’s slipper orchid: Paphiopedilum callosum var. sublaeve. Songklanakarin Journal of Science and Technology, 40(2). DOI: https://doi.org/10.14456/sjst-psu.2018.48
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