Test The Toxicity of The Ethanol Extract of Lacum Leaves (Cayratia trifolia L.) Using The Brine Shrimp Lethality Test (BSLT) Method
DOI:
10.29303/jbt.v23i1.6200Published:
2023-12-20Downloads
Abstract
People use the lacum plant (Cayratia trifolia L.), an herbaceous plant from the Vitaceae family that grows wild, as a traditional medicine. Lacum leaves have special things inside them called secondary metabolites like flavonoids, alkaloids, phenols, steroids, terpenoids, and tannins. Toxicity tests must be carried out because flavonoids, alkaloids and steroids have harmful effects on test animals at certain concentrations. A substance or natural ingredient that will be used as a medicine is tested to determine its level of toxicity. The purpose of this study was to determine the total levels of toxic compounds and the level of toxicity in the ethanol extract of lacum leaves (Cayratia trifolia L.) Brine Shrimp Lethality Test (BSLT). This research uses Artemia salina leach larvae as test subjects. This research also used an experimental approach arranged in a completely randomized design (CRD) consisting of 7 treatments and 4 replications. The percentage of larval death was used in probit analysis to identify the toxic effects of the extract and calculate the LC50 value. Based on the research results, it shows that there are total levels of toxic compounds in the ethanol extract of lacum leaves (Cayratia trifolia L.), namely flavonoids, alkaloids and steroids with an LC50 value of 105.08 ppm which places it in the moderately toxic category and is thought to have anticancer properties.
Keywords:
Artemia salina leach, BSLT, Lacum leaves (Cayratia trifolia L), toxicity, herbal plants.References
Afriani, N., Idiawati, N., & Alimuddin, A. H. (2016). Skrining Fitokimia Dan Uji Toksisitas Ekstrak Akar Mentawa (Artocarpus Anisophyllus) Terhadap Larva Artemia salina. Jurnal Kimia Khatulistiwa.5(1): 58-64. URL: https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/13390/12053
Budiman, F. A., & Hidayat, F. (2021). Uji Toksisitas Akut Ekstrak Etanol Umbi Bit (Beta Vulgaris L.) Dengan Metode Bslt (Brine Shrimp Lethality Test). Jurnal Health Sains. 2(3): 310-315. DOI: https://doi.org/10.46799/jhs.v2i3.129
Chakraborty S, Someshwar S, Kuntal B, Goutam C. (2013). Control of Human FilarialVector, Culex Quinquefasciatus Say 1823 (Diptera: Culicidae) Through Bioactive Fraction of Cayratia trifolia Leaf. Asian Pac J Trop Biomed. 3(12): 980 – 984. DOI: 10.1016/S2221-1691(13)60189-6
Chusniasih, D., & Tutik. (2020). Uji Toksisitas dengan Metode Brine Shrimp Lethality Test (BSLT) dan Identifikasi Komponen Fitokimia Ekstrak Aseton Kulit Buah Kakao (Theobroma cacao L.). Analit: Analytical and Environmental Chemistry. 2(2): 26–31. DOI: http://dx.doi.org/10.23960/aec.v5.i2.2020.p192-201.
Hamidi, M. R., Jovanova, B., & Panovska, T. K. (2014). Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model. Macedonian pharmaceutical bulletin. 60(1): 9-18. DOI: https://doaj.org/article/11973e71e03d4f22af2aa8b26046ffa9
Jelita, S.F., G. W. Setyowati, M. Ferdinand., A. Zuhrotum, dan S. Magantara. (2020). Uji Toksisitas Infus Acalypha Siamensis dengan Metode Brine Shrimp Lethality (BLST). Farmaks vol 18 No.1. URL: https://jurnal.unpad.ac.id/farmaka/article/view/25926
Neliyanti, N. I. (2014). Ekstraksi Dan Uji Stabilitas Zat Warna Alami Dari Buah Lakum (Cayratia trifolia (L.) Domin). Jurnal Kimia Khatulistiwa. 3(2): 30-37. URL: https://jurnal.unpad.ac.id/farmaka/article/view/25926
Potu, valensia valeria. (2021). Brine Shrimp Lethality Test (BSLT) Ekstrak Sarang Lebah Madu (Apis dorsata binghami). Jurnal Pendidikan Biologi Undiksha. 8 (3): 138-144. URL: https://ejournal.undiksha.ac.id/index.php/JJPB/article/view/37506
Prasetyo, B., & Riza Linda, M.. (2016). Pemanfaatan Tumbuhan Lakum (Cayratia trifolia (L.) Domin.) Oleh Etnis Melayu di Kecamatan Sungai Kunyit Kabupaten Mempawah. Jurnal Protobiont. 5(2): 25 -33. DOI: http://dx.doi.org/10.26418/protobiont.v5i2.15929
Purwanto, N., Rismawati, E., & Sadiyah, E. R. (2015). Uji sitotoksik ekstrak biji salak (Salacca Zalacca (Gaert) Voss) Dengan Menggunakan Metode Brine Shrimp lethality test (BSLT) .1(2): 616-622. DOI: http://dx.doi.org/10.29313/.v0i0.2143
Putri, M. K. D., Pringgenies, D., & Radjasa, O. K. (2012). Uji Fitokimia Dan Toksisitas Ekstrak Kasar Gastropoda (Telescopium telescopium) Terhadap Larva Artemia salina. Journal of Marine Research. 1(2): 58-66. DOI: https://doi.org/10.14710/jmr.v1i2.2020
Rumayati, N. I., & Destiarti, L. (2014). Uji Aktivitas Antioksidan, Total Fenol dan Toksisitas dari Ekstrak Daun dan Batang Lakum (Cayratia trifolia (L) Domin). Jurnal Kimia Khatulistiwa. 3(3): 30 – 35. URL: https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/7375
Sasmito, Wiku Adi., Wijayanti Agustina Dwi., Fitriana Ida, P. W. S. (2015). Pengujian Toksisitas Akut Obat Herbal Pada Mencit Berdasarkan Organization for Economic Co- operation and Development (OECD). Jurnal Sain Veteran. 33(2): 234-239. DOI: https://doi.org/10.22146/jsv.17924
Siregar, R. S., Tanjung, A. F., Siregar, A. F., Salsabila, S., Bangun, I. H., & Mulya, M. O. (2021). Studi Literatur Tentang Pemanfaatan Tanaman Obat Tradisional. In Scenario Seminar of Social Sciences Engineering and Humaniora. 385-391. URL: https://jurnal.pancabudi.ac.id/index.php/scenario/article/view/1210
Wardaningrum. (2019). Perbandingan Aktivitas Antioksidan Ekstrak Etanol Terpurifikasi Ubi Jalar Ungu (Ipomoea batata L) dengan Vitamin E’. artikel. 52(1): 1–5.
Widyastuti, Rahmah., Ratnawati, Galuh., & Saryanto. (2019). Penggunaan Tumbuhan Jerango (Acorus Calamus) Untuk Pengobatan Berbagai Penyakit Pada Delapan Etnis Di Provinsi Aceh. Media Konservasi. 24(1): 11-19. DOI:10.29244/medkon.24.1.11-19
Yassir, M., & Meliyana, M. (2019). Jenis Tanaman Obat di Kecamatan Semadam Kabupaten Aceh Tenggara. Serambi Saintia: Jurnal Sains dan Aplikasi. 7(1): 6-12. URL: http://ojs.serambimekkah.ac.id/index.php/serambi-saintia
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