Shoot Induction of Black Stem Potato (Solanum tuberosum L. var. Cingkariang) with Several Concentration of 6-Benzylaminopurine (BAP)
DOI:
10.29303/jbt.v24i1.6237Published:
2024-01-15Downloads
Abstract
Black stem potato (Solanum tuberosum L. var. cingkariang/cingkariang potato) are a specific germplasm of West Sumatra with low productivity. One method of propagating this plant is through tissue culture techniques. The addition of Plant Growth Regulator (PGR) determines the results of plant propagation through tissue culture technique. Cytokinins such as 6-Benzylaminopurine (BAP) play a role in cell division and accelerate shoot formation. This study expects to decide the impact of a few convergences of BAP on the shoot enlistment of cingkariang potato. The examination utilized a totally randomized plan (CRD) with four treatments (0, 1, 2, and 3 mg/L BAP) and six replications. The result showed that BAP was able to induce Cingkariang potato shoots in all concentration. BAP 2 mg/L gave the fastest shoot and root emergence time and also gave the highest number of shoots and roots of Cingkariang potato. BAP 2 mg/L was the best concentration for shoot induction of black stem potato (Solanum tuberosum L. var. Cingkariang).
Keywords:
BAP, cingkariang potato, in vitro, shoot induction, Solanum tuberosum.References
Ahmad, S. S., Tahir, I., Wani, A. S., Dar, R. A., & Nisar, S. (2018). Adenine Type And Diphenyl Urea Derived Cytokinins Improve The Postharvest Performance Of Iris Germanica L. Cut Scapes. Physiology And Molecular Biology Of Plants, 24(6). DOI: https://Doi.Org/10.1007/S12298-018-0554-Z
Akbar, A. M., Eny, F., Sapto, I., & Herawan, T. (2017). Induksi Tunas, Multiplikasi Dan Perakaran. Jurnal Pemuliaan Tanaman Hutan, 11(1). DOI: Https://Doi.Org/10.20886/Jpth.2017.11.1.1-13
Aprilia, M., Setiari, N., & Nurchayati, Y. (2022). Callus Development From Potato (Solanum Tuberosum) Stem At Various Concentrations Of Benzylaminopurine. Biosaintifika, 14(2). DOI: Https://Doi.Org/10.15294/Biosaintifika.V14i2.35703
Arafah, D. L., Hernawati, D., & Nuryadin, E. (2021). The Effect Hormone BAP (6-Benzyl Amino Purine) On The Growth Of Potato Axillary Shoots (Solanum Tuberosum L.) In Vitro. Jurnal Biologi Tropis, 21(3). DOI: Https://Doi.Org/10.29303/Jbt.V21i3.2823
Fehér, A. (2019). Callus, Dedifferentiation, Totipotency, Somatic Embryogenesis: What These Terms Mean In The Era Of Molecular Plant Biology? In Frontiers In Plant Science (Vol. 10). DOI: Https://Doi.Org/10.3389/Fpls.2019.00536
Ferdous, M. H., Masum Billah, A. A., Mehraj, H., Taufique, T., & Jamal Uddin, A. F. M. (2015). BAP And IBA Pulsing For In Vitro Multiplication Of Banana Cultivars Through Shoot-Tip Culture. Journal Of Bioscience And Agriculture Research, 3(2). DOI: Https://Doi.Org/10.18801/Jbar.030215.35
Hajare, S. T., Chauhan, N. M., & Kassa, G. (2021). Effect Of Growth Regulators On In Vitro Micropropagation Of Potato (Solanum Tuberosum L.) Gudiene And Belete Varieties From Ethiopia. Scientific World Journal. DOI: Https://Doi.Org/10.1155/2021/5928769
Hamdani, S. (2020). Teknik Sterilisasi Eksplan Tunas Kentang Granola Kembang (Solanum Tuberosum L.) Untuk Kultur In Vitro. Jurnal Kartika Kimia, 3(2). DOI: Https://Doi.Org/10.26874/Jkk.V3i2.63
Hartati, S., Budiyono, A., & Cahyono, O. (2016). Pengaruh NAA Dan BAP Terhadap Pertumbuhan Subkultur Anggrek Hasil Persilangan Dendrobium Biggibum X Dendrobium Liniale. Caraka Tani: Journal Of Sustainable Agriculture, 31(1). DOI: Https://Doi.Org/10.20961/Carakatani.V31i1.11938
Hoque, M. E., Hena, H., & Ali, M. E. (2022). Potato (Solanum Tuberosum L.) Plantlet Regeneration In Ammonium Nitrate Free Stock Solution-1 Of Murashige & Skoog (MS, 1962) Plant Tissue Culture Medium. European Journal Of Biology And Biotechnology, 3(5). DOI: Https://Doi.Org/10.24018/Ejbio.2022.3.5.413
Ibrahim, M. (2022). Role Of Endogenous And Exogenous Hormones In Bioactive Compounds Production In Medicinal Plants Via In Vitro Culture Technique. In Plant Hormones - Recent Advances, New Perspectives And Applications. DOI: Https://Doi.Org/10.5772/Intechopen.102814
Jumadi, R., & Suhaili, S. (2020). Pertumbuhan Kentang Hitam (Coleus Tuberosum) Varietas Lokal Dari Stek Pada Berbagai Media Tanam. Tropicrops (Indonesian Journal Of Tropical Crops), 3(2). DOI: Https://Doi.Org/10.30587/Tropicrops.V3i2.1830
Karjadi, A. K., & Waluyo, N. (2017). Pengaruh Penambahan BAP Dan GA 3 Terhadap Pertumbuhan Tunas In Vitro Tanaman Kentang ( Solanum Tuberosum L ) ( The Effect Of Addition Of BAP And GA3 On In Vitro Shoot Growth Potato Plant ( Solanum Tuberosum L )). Prosiding Seminar Nasional Pengembangan Teknologi Pertanian, 9–14. DOI: Https://Doi.Org/10.25181/Prosemnas.V0i0.698
Khorsandi, S., Motallebi Azar, A., Zaare Nahandi, F., Hatami, A., & Mokhtarzadeh, S. (2020). Effect Of Different Concentrations Of BAP And Putrescine On Potato Microtuberization (Cv. Agria). Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(2). DOI: Https://Doi.Org/10.21597/Jist.597193
Lekamge, D., Sasahara, T., Yamamoto, S. I., Hatamoto, M., Yamaguchi, T., & Maki, S. (2021). Effect Of Enhanced Cacl2, Mgso4, And KH2PO4 On Improved In Vitro Growth Of Potato. Plant Biotechnology, 38(4). DOI: Https://Doi.Org/10.5511/PLANTBIOTECHNOLOGY.21.0830A
Lestari, F. W., Suminar, E., & Mubarok, S. (2018). Pengujian Berbagai Eksplan Kentang (Solanum Tuberosum L.) Dengan Penggunaan Konsentrasi BAP Dan NAA Yang Berbeda. Jurnal AGRO, 5(1). DOI: Https://Doi.Org/10.15575/1348
Lestari, K. Dwipayani, Ni Wayan Deswiniyanti, Ida Ayu Astarini, & Luh Made Arpiwi. (2019). Callus And Shoot Induction Of Leaf Culture Lilium Longiflorum With NAA And BAP. Nusantara Bioscience, 11(2). DOI: Https://Doi.Org/10.13057/Nusbiosci/N110209
Maninggolang, A., Polii-Mandang, J. S., & Tilaar, W. (2018). Pengaruh Bap (Benzyl Amino Purine) Dan Air Kelapa Terhadap Pertumbuhan Tunas Pucuk Dan Kandungan Sulforafan Brokoli (Brassica Oleracea L. Var. Italica Plenck) Secara In-Vitro. Agri-Sosioekonomi, 14(1). DOI: Https://Doi.Org/10.35791/Agrsosek.14.1.2018.19730
Marta, A., Ukrita, I., Nefri, J., & Darnetti, D. (2023). Faktor-Faktor Yang Mempengaruhi Penurunan Minat Petani Dalam Budidaya Kentang Varietas Cingkariang Di Kabupaten Agam. Journal Of Agribusiness And Community Empowerment (JACE), 6(1). DOI: Https://Doi.Org/10.32530/Jace.V6i1.632
Murashige, T., & Skoog, F. (1962). A Revised Medium For Rapid Growth And Bio Assays With Tobacco Tissue Cultures. Physiologia Plantarum, 15(3), 473–497. DOI: Https://Doi.Org/10.1111/J.1399-3054.1962.Tb08052.X
Premkumar, G., Sankaranarayanan, R., Jeeva, S., & Rajarathinam, K. (2011). Cytokinin Induced Shoot Regeneration And Flowering Of Scoparia Dulcis L. (Scrophulariaceae)-An Ethnomedicinal Herb. Asian Pacific Journal Of Tropical Biomedicine, 1(3). DOI: Https://Doi.Org/10.1016/S2221-1691(11)60020-8
Priya, M. H., & Anbuselvi, S. (2013). Physico Chemical Analysis Of Plectranthus Rotundifolius. Journal Of Chemical And Pharmaceutical Research, 5(3). URL: Https://Www.Jocpr.Com/Articles/Physico-Chemical-Analysis-Of-Plectranthus- Rotundifolius.Pdf
Rana, S. D., Dewi, R. P., Adjie, A. P., & Isda, M. N. (2019). Respons Poliembrioni Dari Biji Duku (Lansium Domesticum Corr.) Yang Dibelah Tiga Secara In Vitro. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati. DOI: Https://Doi.Org/10.24002/Biota.V4i2.2472
Ridwan, Handayani, T., & Witjaksono. (2016). Uji Toleransi Tanaman Kentang Hitam (Plectranthus Rotundifolius (Poir.) Spreng.) Hasil Radiasi Sinar Gamma Terhadap Cekaman Kekeringan. DOI: Https://Doi.Org/10.14203/Jbi.V12i1.2310
Rustikawati, R., Herison, C., Inoriah, E., & Dwisari, V. (2021). Effect Of BAP (6-Benzyl Aminopurine) On In Vitro Shoot Growth Of Curcumas. AGRITROPICA : Journal Of Agricultural Sciences, 4(1). DOI: Https://Doi.Org/10.31186/J.Agritropica.4.1.82-92
Sundari, L., Siregar, L. A. M., & Hanafiah, D. S. (2015). Kajian Awal : Respon Eksplan Nodus Dalam Inisiasi Tunas Mikro Tanaman Karet (Hevea Brasiliensis Muell. Arg.) Dalam Medium WPM. Journal Online Agroteknologi, 3(1).
Widhiastuty, N. S., Anwar, S., & Rosyida. (2023). The Effect Of PVP ( Polivinil Pirolidon) And BAP (6- Benzylamino Purine) On Shoots Induction Of Teak Plus Perhutani ( Tectona Grandis). IOP Conference Series: Earth And Environmental Science, 1246(1), 0–12. DOI: Https://Doi.Org/10.1088/1755-1315/1246/1/012011
Yanti, D., & Isda, M. N. (2021). Induksi Tunas Dari Eksplan Nodus Jeruk Kasturi (Citrus Microcarpa Bunge.) Dengan Penambahan 6- Benzyl Amino Purine (BAP) Secara In Vitro. Biospecies, 14(1). DOI: Https://Doi.Org/10.22437/Biospecies.V14i1.11192
Yuniastuti, E., Praswanto, P., & Harminingsih, I. (2017). Pengaruh Konsentrasi Bap Terhadap Multiplikasi Tunas Anthurium (Anthurium Andraeanum Linden) Pada Beberapa Media Dasar Secara In Vitro. Caraka Tani: Journal of Sustainable Agriculture, 25(1). DOI: https://doi.org/10.20961/carakatani.v25i1.15476
Zainal, A., Anwar, A., Gustian, Fitriawati, & Yunita, R. (2023). The effects of several concentrations of BAP and source of explants to gambier shoot induction ( Uncaria gambier (Hunter) Roxb). IOP Conference Series: Earth and Environmental Science, 1160(1). DOI: https://doi.org/10.1088/1755-1315/1160/1/012021
Zeid, I. M. A., Soliman, H. I. A., & Metwali, E. M. R. (2022). In Vitro Evaluation Of Some High Yield Potato (Solanum Tuberosum L.) Cultivars Under Imposition Of Salinity At The Cellular And Organ Levels. Saudi Journal Of Biological Sciences, 29(4), 2541–2551. DOI: Https://Doi.Org/10.1016/J.Sjbs.2021.12.040
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