Screening of Antimicrobial Producing Bacteria from Berawe Beach Sand on Kampai Pangkalan Susu Island against Pathogenic Bacteria
Authors
Rafika Annisa , Kartika Manalu , Rizki Amelia NasutionDOI:
10.29303/jbt.v24i1.6334Published:
2024-01-11Issue:
Vol. 24 No. 1 (2024): Januari - MaretKeywords:
Antimicrobial, Berawe beach, kampai, pathogenic bacteria, screening.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
Sand is naturally a place to live, grow and develop some marine animals and microorganisms, usually bacteria originating from the sea area that have a large number of bioactive compounds that can produce various kinds of secondary metabolites for further production of antimicrobials. The purpose of this study was to obtain bacteria that have the potential to produce antimicrobials and to characterize bacterial isolates on the sand of Berawe Beach, Kampai Island, Pangkalan Susu. In this study several stages were carried out, namely sampling, isolation, purification, morphological characterization, antimicrobial activity test, gram staining, biochemical test and determining the bacterial genus. The results of this study obtained 9 isolates (SP14A. SP15A, SP16A, SP24A, SP24B, SP35A, SP44A, SP44B and SP45A) which have the potential as antimicrobial producers. Characterization of antimicrobial bacterial isolates from the sand of Berawe Beach, Kampai Island, Pangkalan Susu, namely isolate SP14A, which is a gram- positive bacterium in the form of streptobacilli. SP15A, SP24B and SP44B isolates were coccus-shaped gram-positive bacteria. SP16A, SP35A, SP44A and SP45A isolates were gram-positive bacteria in the form of bacilli. And isolate SP24A is a gram-negative bacterium in the form of streptobacilli. All isolates produce catalase enzymes but do not use carbon and energy. Isolates that are motile (SP14A, SP15A, SP16A, SP24A, SP35A, SP44A and SP45A) can ferment glucose while non-motile (SP24B and SP44B) cannot ferment glucose. These bacteria come from the genera Bacillus, Micrococcus, LactoBacillus and Alcaligenes. Species of Bacillus pumilus, Bacillus subtilis, Bacillus firmus, LactoBacillus bulgaricus, Micrococcus luteus and Alcaligenes eutrophus.
References
Al-Jaelani, M.Z. (2016). Potensi Isolat Rare Actinomycetes Dari Pasir Pantai Barong Gunung Kidul Yogyakarta Sebagai Antibakteri Terhadap Bakteri Escherichia coli. Skripsi. Universitas Muhammadiyah Surakarta. Diakses dari URL: http://eprints.ums.ac.id/id/eprint/46209
Bulan, T.P.L., Erni, J., M. Herdit M., (2021). Daya Tarik Wisata, Motivasi dan Keputusan Berkunjung di Objek Wisata Pantai Berawe. 2(1): 2686– 6064. DOI: https://doi.org/10.32696/jaapi.v2i1.733
Chellaiah, E R. (2018). Cadmium (Heavy Metals) Bioremediation by Pseudomonas aeruginosa: a minireview. Applied Water Science, 8(154). 1-10. DOI: http://dx.doi.org/10.1007/s13201-018-0796-5
Chrisnandari, R.D. (2018). Sintesis dan Karakterisasi Molecularly Imprinted Polymer Untuk Kloramfenikol Menggunakan Polimerisasi Fasa Ruah. Journal of Pharmacy and Science, 3(1): 2527-6328
Davis, W.W. and T.R. Stout. (1971). Disc Plate Method Of Microbiological Antibiotic Assay. Afflied Microbiological. 22(4): 659-665. DOI: https://doi.org/10.1128%2Fam.22.4.659-665.1971
Efstratiou, Maria A. (2018). Mikroorganisme In Beach Sand : Health Limpications. Journal Encylopedia Of Environmental Health. DOI: http://dx.doi.org/10.1016/B978-0-12-409548-9.10914-5
Febriani, H., Ulfa M., dan Efrida P.S.T. (2021). Penapisan Bakteri Penghasil Antimikroba dari Pasir Pantai Sialang Buah Kecamatan Teluk Mengkudu Kabupaten Serdang Bedagai. Journal of Marine Research, 10(4): 560- 564. DOI: https://doi.org/10.14710/jmr.v10i4.31719
Feliatra, I., Efendi, E. Suryadi. (2004). Isolasi dan Identifikasi Bakteri Probiotik dari Ikan Kerapu Macan (Ephinephelus fuscogatus) dalam Upaya Efisiensi Pakan Ikan. Jurnal Natur Indonesia, 6(2): 75-80)
Handayani, W. (2020). Karakterisasi Morfologi Dan Pengelompokan Isolat Bakteri Penghasil Hormon IAA (Indole Acetic Acid) Dari Tanah Rhizosfer Bawang Merah (Allium cepa) Di Nganjuk Dengan Variasi Wilayah Yang Berbeda. Skripsi. Universitas Islam Negeri Sunan Ampel
Hasyimi, M. (2010). Mikrobiologi Untuk Mahasiswa Kebidanan. Jakarta : CV Trans Info Media
Kurnianto, M.A, dkk. (2020). Penapisan Antibacteria Akuatik Penghasil Antibakteri dari Ikan Bandeng dan Belanak dengan Metode Double Layer Diffusion. Jurnal Kedokteran dan Perikanan. DOI: http://dx.doi.org/10.15578/jpbkp.v15i1.647
Lubis, S.S. (2015). Penapisan Bakteri Laut Penghasil Antimikroba Dari Pesisir Serdang Bedagai Sumatera Utara. Ikawnie : Journal Of Islamic Science and Technology. 1(1); 87-89
Mikdarullah dan Aditiya N. (2017). Teknik Isolasi Bakteri Proteolitik Dari Sumber Air Panas Ciwidey Bandung. Buletin Teknik Litkayasa Akuakultur, 15(1): 11-14. DOI: http://dx.doi.org/10.15578/blta.15.1.2017.11-14
Suciari, L.K., Nyoman M., Cok. D.W.HS. (2017). Perbedaan Zona Hambat Pertumbuhan Staphylococcus aureus Pada Berbagai Konsentrasi Rebusan Daun Salam (Syzygium polyanthum) Secara In Vitro. Meditoty, 5(2): 92 – 100. DOI: https://doi.org/10.33992/m.v5i2.138
Suriani & Muis, A. (2016). Prospek Bacillus subtilis Sebagai Agen Pengendali Hayati Patogen Tular Tanah Pada Tanaman Jagung. Jurnal Litbang Pert, 35(1), 37-45. DOI: https://dx.doi.org/10.21082/jp3.v35n1.2016.p37-45
Thoyib, H., Ratna S., dan Suranto. (2007). Seleksi dan Identifikasi Bakteri Alkalifilik Penghasil Xilanase dari Tanah Bukit Krakitan, Bayat, Klaten. Jurnal Bioteknologi, 4(1): 6-12. DOI: 10.13057/biotek/c040102
Ulfa, A., Endang, S., dan Mimien, H.I.M. (2016). Isolasi Dan Uji Sensitivitas Merkuri Pada Bakteri Dari Limbah Penambangan Emas Di Sekotong Barat Kabupaten Lombok Barat : Penelitian Pendahuluan. Proceeding Biology Education Conference. 13(1): 793-799. Diakses dari https://jurnal.uns.ac.id/prosbi/article/view/5916
Yahya., Nursyam, H., Risjani, Y & Soemarno. (2014). Karakteristik Bakteri di Perairan Mangrove Pesisir Kraton Pasuruan. Ilmu Kelautan, 19(1), 35- 42. DOI: http://dx.doi.org/10.14710/ik.ijms.19.1.35-42
Yulianti, E., dan Anna R., dkk. (2015). Optimasi Produksi Senyawa Antimikroba Pada Cell Free Ekstrak Hasil Fermentasi Bakteri Termofilik Pasca Erupsi Merapi. Yogyakarta: FMIPA. 4(2).. DOI: http://dx.doi.org/10.21831/jsd.v4i2.9089
Zulfiati, A. (2018). Isolasi Mikroba Penghasil Antibiotik Dari Pasir Pantai Lemo- Lemo Kabupaten Bulukumba Dalam Menghambat Beberapa Bakteri Patogen. Jurnal Kesehatan. Vol 13(1). DOI: https://doi.org/10.24252/kesehatan.v13i1.13740
License
Copyright (c) 2024 Rafika Annisa, Kartika Manalu, Rizki Amelia Nasution
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.