Antibacterial Activity of Endophytic Fungi Isolated from Pulai Leaves (Alstonia scholaris)

Mira Rumalolas; Sarmawaty Kotala; Asyik Nur Allifah AF

doi:10.29303/jbt.v24i1.6370

Antibacterial Activity of Endophytic Fungi Isolated from Pulai Leaves (Alstonia scholaris)

Authors

Mira Rumalolas , Sarmawaty Kotala , Asyik Nur Allifah AF

DOI:

10.29303/jbt.v24i1.6370

Published:

2023-01-15

Issue:

Vol. 24 No. 1 (2024): Januari - Maret

Keywords:

Antibacterial, endophytic fungi, pulai leaves.

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Rumalolas, M., Kotala, S., & AF, A. N. A. (2023). Antibacterial Activity of Endophytic Fungi Isolated from Pulai Leaves (Alstonia scholaris). Jurnal Biologi Tropis, 24(1), 85–91. https://doi.org/10.29303/jbt.v24i1.6370

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Abstract

Extraction of active compounds from plants requires a lot of raw materials. Therefore, other alternatives are needed that can produce active compounds in a relatively fast time. One of them is the use of endophytic fungi. Endophytic fungi live in plant tissues including pulai (Alstonia scholaris) which is used as an ingredient in herbal medicine. Endophytic growths can create auxiliary metabolite intensifies that are like their host plants. The point of this examination was to decide the sorts of endophytic growths found on pulai leaves and their antibacterial action. This research is a qualitative research, where microscopic characteristics of fungi are observed using the slide culture method and antibacterial activity is tested using the modified Kirby Bauer method. The results of this research found 8 isolates of endophytic fungi, consisting of Neurospora sp¹, Neurospora sp², Paecilomyces sp, Acremonium sp, Fusarium sp, and 3 unidentified isolates. All endophytic fungi found on pulai leaves can inhibit the growth of Staphylococcus aureus and Escherichia coli with the highest inhibition zone diameter found in isolate K8 (Acremonium sp) at 3 cm for S. aureus and isolate K7 at 1.8 cm for E. coli.

References

Asim, S., Hussain, A., Murad, W., Hamayun, M., Iqbal, A., Rehman, H., Tawab, A., Irshad, M., Alataway, A., Dewidar, A. Z., Elansary, H. O., & Lee, I. J. (2022). Endophytic Fusarium oxysporum GW Controlling Weed and An Effective Biostimulant for Wheat Growth. Frontiers in Plant Science, 13. DOI: https://doi.org/10.3389/fpls.2022.922343

Botek, M. (2020). Pengaruh Paecilomyces sp. pada Berbagai Bahan Organik terhadap Ketahanan dan Produksi Padi Gogo. Jurnal Agercolere, 2(2), 30–36. DOI: https://doi.org/10.37195/jac.v2i2.91

Demeni, P. C. E., Betote, P. H. D., Kom, C. W., Tchamgoue, E. N., Ndedi Moni, E. D. F., Foumane Maniepi, J. S., Agbor, G. A., & Nyegue, M. A. (2021). Endophytic Fungi from Alstonia boonei de Wild and Greenwayodendron suaveolens (Engl. And Diels) Verdc. subsp. Suaveolens Possess Inhibitory Activity against Pneumonia Causing Bacteria. Evidence-Based Complementary and Alternative Medicine, 2021. DOI: https://doi.org/10.1155/2021/9966323

Deshmukh, S. K., Dufossé, L., Chhipa, H., Saxena, S., Mahajan, G. B., & Gupta, M. K. (2022). Fungal Endophytes: A Potential Source of Antibacterial Compounds. In Journal of Fungi (Vol. 8, Issue 2). DOI: https://doi.org/10.3390/jof8020164

Devi, D., Anggraeni, A., & Wahyuni, T. (2021). Isolasi Kapang Endofit Pelawan (Tristaniopsis merguensis Griff.) yang Berpotensi Sebagai Antibakteri Terhadap Escherichia coli dan Staphylococcus aureus. Al-Kauniyah: Jurnal Biologi, 14(2), 195–206. DOI: https://doi.org/10.15408/kauniyah.v14i2.14051

Elfita, Mardiyanto, Fitrya, Eka Larasati, J., Julinar, Widjajanti, H., & Muharni. (2019). Antibacterial Activity of Cordyline Fruticosa Leaf Extracts and Its Endophytic Fungi Extracts. Biodiversitas, 20(12), 3804–3812. DOI: https://doi.org/10.13057/biodiv/d201245

Fitriani, R. & Putrie, W. (2015). Mikroba Endofitik Tanaman, Primadona yang Tidak Kasat Mata. BioTrends, 1(1), 9–13.

Hasiani, V. V., Ahmad, I., & Rijai, L. (2015). Isolasi Jamur Endofit dan Produksi Metabolit Sekunder Antioksidan dari Daun Pacar (Lawsonia inermis L.). Jurnal Sains dan Kesehatan, 1(4), 146–153. DOI: https://doi.org/10.25026/jsk.v1i4.32

Hawar, S. N., Taha, Z. K., Hamied, A. S., Al-Shmgani, H. S., Sulaiman, G. M., & Elsilk, S. E. (2023). Antifungal Activity of Bioactive Compounds Produced by the Endophytic Fungus Paecilomyces sp. (JN227071.1) against Rhizoctonia solani. International Journal of Biomaterials, 2023. DOI: https://doi.org/10.1155/2023/2411555

Huda, N., Imaningsih, W., & Hakim, S. S. (2019). Uji Antagonisme Kapang Endofit Tanaman Galam (Melaleuca cajuputi) terhadap Colletotrichum truncatum. Jurnal Mikologi Indonesia, 3(2), 59. DOI: https://doi.org/10.46638/jmi.v3i2.62

Indrawangsa, G. D., Sudarma, I. M., & Singarsa, I. D. P. (2017). Uji Daya Hambat Jamur Endofit terhadap Phytophthora palmivora (Butler) Butler Penyebab Penyakit Busuk Buah Kakao Secara In Vitro. E-Jurnal Agroekoteknologi Tropika, 6(3), 229–238. URL: https://ojs.unud.ac.id/index.php/JAT

Khan, M. I., Ahhmed, A., Shin, J. H., Baek, J. S., Kim, M. Y., & Kim, J. D. (2018). Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study in Vitro and in Vivo. Evidence-Based Complementary and Alternative Medicine, 2018. DOI: https://doi.org/10.1155/2018/3486106

Khan, M. S., Gao, J., Munir, I., Zhang, M., Liu, Y., Moe, T. S., Xue, J., & Zhang, X. (2021). Characterization of Endophytic Fungi, Acremonium sp., from Lilium davidii and Analysis of Its Antifungal and Plant Growth-Promoting Effects. BioMed Research International, 2021. DOI: https://doi.org/10.1155/2021/9930210

Kuncoro, H., & Erma Sugijanto, N. (2011). Jamur Endofit, Biodiversitas, Potensi dan Prospek Penggunaannya Sebagai Sumber Bahan Obat Baru. Journal Of Tropical Pharmacy And Chemistry, 1(3), 250–265. DOI: https://doi.org/10.25026/jtpc.v1i3.35

Lestari, Y., Ardiningsih, P., & Nurlina. (2016). Aktivitas Antibakteri Gram Positif dan Negatif dari Ekstrak dan Fraksi Daun Nipah (Nypa fruticans Wurmb.) Asal Pesisir Sungai Kakap Kalimantan Barat. Jkk, 5(4), 1–8.

Nurhasanah, Gultom, E.S. (2020). Uji Aktivitas Antibakteri Ekstrak Metanol Daun Kirinyuh (Chromolaena odorata) Terhadap Bakteri MDR (Multi Drug Resistant) dengan Metode Klt Bioautografi. Jurnal Biosains, 6 (2), 45-52. DOI: https://doi.org/10.24114/jbio.v6i2.16600

Posangi, J. & Bara, R. A. (2014). Analisis Aktivitas dari Jamur Endofit Yang Terdapat dalam Tumbuhan Bakau Avicennia marina di Tasik Ria Minahasa. Jurnal Pesisir Dan Laut Tropis, 2(1), 30. DOI: https://doi.org/10.35800/jplt.2.1.2014.7345

Pratiwi, R. H. (2019). Peranan Mikroorganisme Endofit Dalam Dunia Kesehatan: Kajian Pustaka. Sainmatika: Jurnal Ilmiah Matematika Dan Ilmu Pengetahuan Alam, 16(1), 21. DOI: https://doi.org/10.31851/sainmatika.v16i1.2695

Ramadhani, S.H., Samingan, & Iswadi. (2017). Isolation and Identification of Endophytic Fungi in Leaves of Jamblang (Syzygium cumini L.). Jurnal Ilmiah Mahasiswa Fakultas Keguruan Dan Ilmu Pendidikan Unsyiah, 2(2), 77–90.

Ramos, G. da C., Silva-Silva, J. V., Watanabe, L. A., Siqueira, J. E. de S., Almeida-Souza, F., Calabrese, K. S., Marinho, A. M. do R., Marinho, P. S. B., & Oliveira, A. S. de. (2022). Phomoxanthone A, Compound of Endophytic Fungi Paecilomyces sp. and Its Potential Antimicrobial and Antiparasitic. Antibiotics, 11(10), 1–14. DOI: https://doi.org/10.3390/antibiotics11101332

Rahmawatiani, A., Mayasari, D., Narsa, A.C. 2020. Kajian Literatur: Aktivitas Antibakteri Ekstrak Herba Suruhan (Peperomia pellucida L.). Proceeding of Mulawarman Pharmaceuticals Conferences. e-ISSN: 2614-4778. DOI: https://doi.org/10.25026/mpc.v12i1.401

Richter, M.F. & Hergenrother, P.J. 2019. The Challenge Of Converting Gram-Positive-Only Compounds Into Broad-Spectrum Antibiotics. Ann N Y Acad Sci. 1435(1): 18–38. DOI: https://doi.org/10.1111/nyas.13598

Silalahi, M. (2019). Botani dan Bioaktivitas Pulai (Alstonia scholaris). Jurnal Pro-Life, 6(2), 136–147. DOI: https://doi.org/10.33541/jpvol6Iss2pp102

Widowati, R., Handayani, S., & Al Fikri, A. R. (2021). Phytochemical Screening and Antibacterial Activities of Senggani (Melastoma malabathricum L.) Ethanolic Extract Leaves. Jurnal Ilmu Pertanian Indonesia, 26(4), 562–568. DOI: https://doi.org/10.18343/jipi.26.4.562

Yan, Y., Li, X., Zhang, C., Lv, L., Gao, B., & Li, M. (2021). Research Progress On Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review. Antibiotics, 10(3). DOI: https://doi.org/10.3390/antibiotics10030318

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Antibacterial Activity of Endophytic Fungi Isolated from Pulai Leaves (Alstonia scholaris)

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