Detection of Candida albicans in Urine Patients of Diabetes Melitus using Polymerase Chain Reaction (PCR) Method
DOI:
10.29303/jbt.v24i3.7173Published:
2024-07-25Downloads
Abstract
Candida albicans is a dimorphic fungus that can grow in culture as a blastopore or as pseudohyphae. Laboratory diagnosis of the Candida albincans fungus can be carried out molecularly, which aims to identify DNA in the fungus being examined and carried out by going through the initial molecular stage, namely DNA isolation. The purpose of this study was to determine whether the fungus Candida albicans was present in urine isolate samples from diabetes mellitus sufferers at the Wonosari 1 Klaten Community Health Center. The research method used is descriptive. The samples used were urine samples from diabetes mellitus sufferers at the Wonosari 1 Community Health Center. Based on the research results, data analysis and discussion in this study, it can be concluded that the Candida albicans fungus can be detected in the ITS2 region at a product length of 107bp using the Polymerase Chain Reaction (PCR) method.
Keywords:
Candida albicans, DNA isolation, ITS2.References
Akbar, B. M. (2018). Gambaran Candida albicans Pada Urin Penderita Diabetes Mellitus Di Rumah Sakit Bhayangkara. Repository Poltekkes Kemenkes Palembang, accessed April 10,2023.
Aristya, G.R. Agriansyah, A. Daryono, B.S. (2013). Deteksi dan Skrining Pewarisan Sifat Ketahanan Penyakit Powdery Mildew pada Generasi Backross Tanaman Melon (Cucumis melo L.) Var Tacapa. Fakultas Biologi. Universitas Gadjah Mada. Yogyakarta.
Az-zahro, F., Kristinawati, E., & Fikri, Z. (2021). Hubungan Antara Kandidiasis Pada urine Wanita Penderita Diabetes Mellitus dengan Nilai Positivitas Glukosuria di Wilayah Kerja Puskesmas Narmada. Jurnal Analis Medika Biosains (JAMBS), 8(2), 92–98. https://doi.org/10.32807/jambs.v8i2.239
Dewanata, P. A., & Mushlih, M. (2021). Differences in DNA Purity Test Using UV-Vis Spectrophotometer and Nanodrop Spectrophotometer in Type 2 Diabetes Mellitus Patients .vol 15. https://doi.org/10.21070/ijins.v15i.553
Fatchiyah, 2011. Dasar-Dasar Teknik Analisa Biologi Molekuler. Jakarta : Erlangga
Helmawati. (2021). Cegah Diabets Sebelum Terlambat. Editor Herman Adamson. Yogyakarta : Healthy. Diakses pada tanggal 20 Juli 2021.
Imam, M. P., & Sriwidodo. (2019). Buku Teknik Biologi Molekular (Issue September) Departemen Kimia FMIPA Universitas Padjadjaran,109.
Indrayati, S., & Afriani STIKes Perintis Padang, M. (2018). Jurnal Kesehatan Perintis (Perintis’s. Health Journal, 5(2). https://doi.org/10.33653/jkp.v5i2.146
Komalasari, K. 2019. Pengaruh Perbandingan Volume Darah Dan Lisis Buffer
Serta Kecepatan Sentrifugasi Terhadap Kualitas Produk DNA Pada
Sapi Friesian Holstein (Fh). Institut Pertanian Bogor. Skripsi.
Departemen Ilmu Produksi Dan Teknologi Peternakan Fakultas
Peternakan Institut Pertanian Bogor.
Muhajir, N. F., Nadifah, F., Arisandi, D., & Susliyanti, M. (2022). Identifikasi Candida Sp Dalam Urine Penderita Diabetes Mellitus Tipe 2 Di Puskesmas Ngemplak 2 Kabupaten Sleman Yogyakarta. Jurnal Mitra Kesehatan, 3(1), 41–46. https://doi.org/10.47522/jmk.v3i1.50
Riset Kesehatan Dasar (Riskesdas) (2018). Badan Penelitiandan Pengembangan Kesehatan Kementerian RI tahun 2018.http://www.depkes.go.id/resources/download/infoterkini/materi_rakorp2018/Hasil%20Riskesdas%202018.pdf – Diakses Agustus 2018.
Souza, A. L. (2022). Prevalence , Molecular Identification , and Genotyping of Candida Species Recovered from Oral Cavity among Patients with Diabetes Mellitus from. 1–8. https://doi.org/10.4103/abr.abr
Suharsono & Widyastuti. 2016. Penuntun Prak- tikum Pelatihan Teknik Pengklonan Gen. Pusat Penelitian Sumber Daya Hayati dan Bioteknologi. IPB
Suwitra, K. (2019). Penyakit Ginjal Kronik. Dalam: Buku Ajar Ilmu Penyakit Dalam. Jilid III. Balai Penerbit FKUI. Jakarta. Hlm. 1035-1040
Welliangan M, Wowor M, Mongan A. (2019). Gambaran kadar glukosa urin pada primagravida dengan orang tua penyandang diabetes melitus di Kota Manado. eBiomedik, 7(1):19-23. https://doi.org/10.35790/ebm.7.1.2019.22621
Yuenleni. (2019). Langkah-langkah Optimasi PCR. Indonesian Journal of Laboratory. 1(3): 51-56. https://doi.org/10.22146/ijl.v1i3.48723
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