Standardization of Specific and Non-Specific Parameters of Methanol Extract from Poinsettia Red Leaves (Euphorbia pulcherrima Willd.)
DOI:
10.29303/jbt.v24i3.7412Published:
2024-08-30Downloads
Abstract
The poinsettia red leaves (Euphorbia pulcherrima Willd.) have the potential as antibacterial and antioxidant agents and are widely utilized by the community for wound healing purposes. This research aims to determine the specific and non-specific parameter values of methanol extract from poinsettia red leaves. Samples of poinsettia red leaves were processed into extracts using the maceration method with 96% methanol as the solvent. The extract was standardized by determining specific and non-specific parameters. The test result data were analyzed descriptively in both qualitative and quantitative manners. The standardization results of specific parameters showed the identity of the extract as methanol extract of poinsettia red leaves, with organoleptic properties of a thick greenish-black extract, distinctive poinsettia aroma, and bitter taste. The water-soluble compound content was 51,47% ± 0,437 and ethanol-soluble 61,22% ± 0,117. Results for non-specific parameters indicated drying loss of 12,50% ± 0,419, moisture content of 12,62% ± 0,173, total ash content of 5,25% ± 0,538, acid-insoluble ash of 0,34% ± 0,048, residual solvent 0,00% ± 0,000, total plate count was TFTC (Too Few To Count), and mold and yeast count was 1,5 x 104 colonies/g sample. This research can serve as a reference for determining specific and non-specific parameters of methanol extract from poinsettia red leaves.
Keywords:
Extract, standardization, poinsettia red leaves.References
Adawiah, Sukandar, D., & Muawanah, A. (2015). Aktivitas Antioksidan dan Kandungan Komponen Bioaktif Sari Buah Namnam. Jurnal Kimia VALENSI, 1 (2): 130-136. DOI: http://doi.org/10.15408/jkv.v0i0.3155
Affandy, F. (2020). Skrining Fitokimia dan Penetapan Kadar Fenolik Total pada Tanaman Penyembuh Luka di Lombok Timur (Skripsi). Universitas Mataram.
Affandy, F., Wirasisya, D. G., & Hanifa, N. I. (2021). Skrining Fitokimia pada Tanaman Penyembuh Luka di Lombok Timur. Sasambo Journal of Pharmacy, 2 (1): 1-6. DOI: https://doi.org/10.29303/sjp.v2i1.84
Andriani, D., & Murtisiwi, L. (2018). Penetapan Kadar Fenolik Total Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) dengan Spektrofotometri UV Vis. Cendekia Journal of Pharmacy, 2 (1): 32-38. DOI: https://doi.org/10.31596/cjp.v2i1.15
BPOM RI. (2017). Peraturan Badan Pengawas Obat dan Makanan Tahun 2017 tentang Pelarut yang Diizinkan Digunakan dalam Proses Ekstraksi/Fraksinasi Tumbuhan dalam Produk Obat Bahan Alam dan Suplemen Kesehatan beserta Batasan Residunya. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia.
BPOM RI. (2019). Peraturan Badan Pengawas Obat dan Makanan Nomor 32 Tahun 2019 tentang Persyaratan Keamanan dan Mutu Obat Tradisional. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia.
Depkes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan RI.
Evans, E., & Davis, J. (2019). Harvesting and Preserving Herbs for The Home gardener. Carolina Utara: NC State Extension.
Humas BKPK. (2022). Fitofarmaka Menjadi Unggulan Produk Dalam Negeri. Jakarta: Kementerian Kesehatan Republik Indonesia.
Ibrahim, A. T., Sukenti, K., & Wirasisya, D. G. (2019). Uji Potensi Antimikroba Ekstrak Metanol Daun Kastuba (Euphorbia pulcherrima Willd.). Natural B, 5 (1): 13-18.
Ifadah, R. A., Wiratara, P. R. W., & Afgani, C. A. (2021). Ulasan Ilmiah: Antosianin dan Manfaatnya untuk Kesehatan. Jurnal Teknologi Pengolahan Pertanian, 3 (2): 11-21. DOI: https://doi.org/10.35308/jtpp.v3i2.4450
Kemenkes RI. (2017). Farmakope Herbal Indonesia Edisi II. Jakarta: Kementerian Kesehatan RI.
Kemenkes RI. (2020). Farmakope Indonesia Edisi VI. Jakarta: Kementerian Kesehatan RI.
Mahasuari, N. P. S., Paramita, N. L. P. V., & Putra, A. A. G. R. Y. (2020). Effect of Methanol Concentration as A Solvent on Total Phenolic and Flavonoid Content of Beluntas Leaf Extract (Pulchea indica L.). Journal of Pharmaceutical Science and Application, 2 (2): 77-84. DOI: https://doi.org/10.24843/JPSA.2020.v02.i02.p05
Marpaung, M. P., & Septiyani, A. (2020). Penentuan Parameter Spesifik dan NonSpesifik Ekstrak Kental Etanol Batang Akar Kuning (Fibraurea chloroleuca Miers). Journal of Pharmacopolium, 3 (2): 58-67. DOI: http://dx.doi.org/10.36465/jop.v3i2.622
Maryam, F., Taebe, B., & Toding, D. P. (2020). Pengukuran Parameter Spesifik dan Non Spesifik Ekstrak Etanol Daun Matoa (Pometia pinnata J.R & G.Forst.). Jurnal Mandala Pharmacon Indonesia, 6 (1): 1-12. DOI: https://dx.doi.org/10.35311/jmpi.v6i01.39
Moustaka, J., Tanou, G., Giannakoula, A., Adamakis, I. S., Panteris, E., Eleftheriou, E. P., & Moustakas, M. (2020). Anthocyanin Accumulation in Poinsettia Leaves and Its Functional Role in Photo-Oxidative Stress. Environmental and Experimental Botany, 175: 1-13. DOI: https://doi.org/10.1016/j.envexpbot.2020.104065
Pertiwi, R., & Wulandari, S. (2022). Buku Ajar: Farmakognosi Simplisia Minyak Atsiri dan Gula. Jawa Tengah: Lakeisha.
Roni, K. A., & Legiso. (2021). Kimia Organik. Palembang: NoerFikri Offset.
Sharif, H. B., Mukhtar, M. D., Mustapha, Y., Baba, G., & Lawal, A. O. (2015a). Acute and Subchronic Toxicity Profile of Euphorbia pulcherrima Methanol Extract on Wistar Albino Rats. Advances in Pharmaceutics, 2015 (4): 1-9. DOI: http://dx.doi.org/10.1155/2015/539646
Sharif, H. B., Mukhtar, M. D., Mustapha, Y., & Lawal, A. O. (2015b). Preliminary Investigation of Bioactive Compounds and Bioautographic Studies of Whole Plant Extract of Euphorbia pulcherrima on Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa. Advances in Pharmaceutics, 2015 (25): 1-14. DOI: http://dx.doi.org/10.1155/2015/485469
Sopiah, B., Muliasari, H., & Yuanita, E. (2019). Skrining Fitokimia dan Potensi Aktivitas Antioksidan Ekstrak Etanol Daun Hijau dan Daun Merah Kastuba. Jurnal Ilmu Kefarmasian Indonesia, 17 (1): 27-33. DOI: https://doi.org/10.35814/jifi.v17i1.698
Supriningrum, R., Fatimah, N., & Purwanti, Y. E. (2019). Karakterisasi Spesifik dan Non Spesifik Ekstrak Etanol Daun Putat (Planchonia valida). Al-Ulum: Jurnal Sains dan Teknologi, 5 (1): 6-12. DOI: http://doi.org/10.31602/ajst.v5i1.2468
Weisenhorn, J. (2014). Growing and Caring for Poinsettia. Minneapolis: University of Minnesota Extension.
Wirasisya, D. G., Hamid, A., Haikhal, M., Hidayati., A. R., Sunarwidhi, A. L., & Hanifa, N. I. (2023). Anti-Stapylococcus Epidermidis of Methanolic Extracts from Some East Lombok Medical Plants. Jurnal Biologi Tropis, 23 (2): 1-8. DOI: http://doi.org/10.29303/jbt.v23i2.4732
License
Copyright (c) 2024 Lalu Khairi Abdillah, Nisa Isneni Hanifa, Neneng Rachmalia Izzatul Mukhlishah, Agriana Rosmalina Hidayati
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
