Anti-Radical Activity Test of Fractions from Ashitaba Herbs (Angelica keiskei)

Ni Made Ayu Dinda Permatasari; Handa Muliasari; Nisa Isneni Hanifa; Agriana Rosmalina Hidayati; Dedianto Hidajat; Wahida Hajrin; Adila Rizkika

doi:10.29303/jbt.v24i4.7638

Anti-Radical Activity Test of Fractions from Ashitaba Herbs (Angelica keiskei)

Authors

Ni Made Ayu Dinda Permatasari , Handa Muliasari , Nisa Isneni Hanifa , Agriana Rosmalina Hidayati , Dedianto Hidajat , Wahida Hajrin , Adila Rizkika

DOI:

10.29303/jbt.v24i4.7638

Published:

2024-10-25

Issue:

Vol. 24 No. 4 (2024): Oktober - Desember

Keywords:

Angelica keiskei, antioxidants, DPPH, flavonoids, phenolics.

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Permatasari, N. M. A. D., Muliasari, H., Hanifa, N. I., Hidayati, A. R., Hidajat, D., Hajrin, W., & Rizkika, A. (2024). Anti-Radical Activity Test of Fractions from Ashitaba Herbs (Angelica keiskei). Jurnal Biologi Tropis, 24(4), 492–500. https://doi.org/10.29303/jbt.v24i4.7638

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Abstract

Acne vulgaris is a chronic inflammation of the skin’s pilosebaceous unit, caused by oxidative stress due to reactive oxygen species (ROS) release. Oxidative stress conditions can be treated using natural antioxidants, namely ashitaba (Angelica keiskei). In contrast to previous studies that tested column chromatography fraction of the macerated ethanol extract, this study examined the liquid-liquid partition fraction of 80% methanol sonicated extract of Ashitaba herb. This study aims to determine the antiradical activity of various fractions of Ashitaba herb extract against 1,1-diphenyl-2-picrylhidrazyl (DPPH) free radicals. 80% methanol extract of the Ashitaba herb was fractionated using chloroform, ethyl acetate, and water solvents. Each ashitaba herb fraction was identified for the content of flavonoids and phenolic compounds and the antiradical DPPH activity was tested with ascorbic acid standard using a UV-Vis spectrophotometer. The IC₅₀ value of each fraction was statistically analyzed using One-Way ANOVA and Posthoc Tukey. The results of phytochemical identification showed that ashitaba herb fractions positively contain flavonoids and phenolics. Antiradical activity of the chloroform, ethyl acetate, and water fractions against DPPH was indicated by IC₅₀ values of 164.22±5.68 ppm (moderate); 8.70±0.12 ppm (very strong); and 243.60±8.14 ppm (weak). Statistically, each ashitaba herb fraction differed significantly from ascorbic acid (IC₅₀ 2.37±0.05 ppm). The ethyl acetate fraction has the strongest antioxidant activity and is in the same category of antioxidant strength as the ascorbic acid standard.

References

Ahmed, F., & Iqbal, M. (2018). Antioxidant activity of Ricinus Communis. Organic & Medicinal Chemistry International Journal, 5(3), 1–6. https://doi.org/10.19080/omcij.2018.05.555667

Alawiyah, A. L., & Senania, A. (2022). Antioxidant Activity and Bioactive Compounds of Ethyl Acetate Fractions from Syzygium cumini Wood Stem. ALKIMIA : Jurnal Ilmu Kimia dan Terapan, 5(1), 93–101. https://doi.org/10.19109/alkimia.v5i1.7143

Anwar, F., Qayyum, H. M. A., Hussain, A. I., & Iqbal, S. (2010). Antioxidant Activity of 100% and 80% Methanol Extracts from Barley Seeds (Hordeum vulgare L.): Stabilization of Sunflower Oil. Grasas y Aceites, 61(3), 237–243. https://doi.org/10.3989/gya.087409

Athallah, M. D. N. (2024). Uji Aktivitas Antioksidan, Antiinflamasi Dan Analgetik Ekstrak Etanol Daun Ashitaba (Angelica Keiskei) Terhadap Mencit (Mus Musculus). Program Studi S1 Farmasi Fakultas Farmasi Universitas Bakti Tunas Husada Tasikmalaya.

Blois, M. S. (1958). Antioxidant Determinations by The Use of A Stable Free Radical. Nature, 4617(1), 1–2.

Dewi, N. L. A., Adnyani, L. P. S., Pratama, R. B. R., Yanti, N. N. D., Manibuy, J. I., & Warditiani, N. K. (2018). Pemisahan, Isolasi, dan Identifikasi Senyawa Saponin dari Herba Pegagan (Centella asiatica L. Urban). Jurnal Farmasi Udayana, 7(2), 68–76.

Evans, E., & Davis, J. (2019). Harvesting Herbs Harvesting and Preserving Herbs for the Home Gardener. https://content.ces.ncsu.edu/harvesting-and-preserving-herbs-for-the-home-gardener#:~:text=Harvest

Gandjar, I. G., & Rohman, A. (2012). Kimia Farmasi Analisis (2nd ed.). Pustaka Pelajar.

Garem, Y. F. El, Ahmed, R. A. M., Ragab, M. A., & AbouZeid, A. A. (2014). Study of Oxidative Stress in Different Clinical Severities of Acne Vulgaris. Egyptian Journal of Dermatology and Venerology, 34(1), 53. https://doi.org/10.4103/1110-6530.137313

Gulcin, İ. (2020). Antioxidants and Antioxidant Methods: an Updated Overview. Archives of Toxicology, 94(3), 651–715. https://doi.org/10.1007/s00204-020-02689-3

Hajrin, W., Juliantoni Y. (2019). Formulasi Lotion Esktrak Etanolik Herba Ashitaba (Angelica Keiskei) sebagai Penangkal Radikal Bebas. Unram Medical Journal, 8(2),5.https://doi.org/10.29303/jku.v8i2.335

Harbone, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (3rd ed.). Chapman & Hall.

Hidajat, D., Hidayati, A. R., & Cenderadewi, M. (2016). Karakteristik Pengetahuan dan Persepsi Penderita Akne Vulgaris di Kota Mataram. Jurnal Kedokteran Unram, 5(4), 4–10.

Hosthota, A., Bondade, S., & Basavaraja, V. (2016). Impact of Acne Vulgaris on Quality of Life and Self-esteem. Cutis, 98(2), 121–124.

Ipandi, I., Triyasmono, L., & Prayitno, B. (2016). Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Kajajahi (Leucosyke capitellata Wedd.). Jurnal Pharmascience, 3(1), 93–100.

Isnindar, I., & Luliana, S. (2020). Synergism of Antioxidant Activity Combination of Buas-Buas (Premnaserratifolia Linn.), Meniran (Phyllanthusniruri L.), Secang (Caesalpiniasappan) and Roselle (Hibiscus sabdarifa) Extracts. Traditional Medicine Journal, 25(3), 140–145. https://doi.org/10.22146/mot.51328

Julizan, N., Maemunah, S., Dwiyanti, D., & Al Anshori, J. (2019). Validasi Penentuan Aktifitas Antioksidan dengan Metode

Lestari, Y. D., Permatasari, S., & Oktasari, &ade. (2020). Antioxidant Activity Testing of Extract Kweni Peel (Mangifera odorata Griff). Indonesian Journal of Chemistry and Environment, 3(2), 11–20.

Li, X., Wu, X., & Huang, L. (2009). Correlation Between Antioxidant Activities and Phenolic Contents of Radix Angelicae Sinensis (Danggui). Molecules, 14(12), 5349–5361. https://doi.org/10.3390/molecules14125349

Muliasari, H., Hanifa, N. I., Hajrin, W., Andanalusia, M., & Hidayati, A. R. (2023). Determination of Antioxidants by DPPH Scavenging Activity of Ashitaba Herb (Angelica keiskei) Methanol Extract. Jurnal Biologi Tropis, 23(4), 482–490. https://doi.org/10.29303/jbt.v23i4.5686

Nasution, P. A., Batubara, R., & Surjanto, S. (2015). Tingkat Kekuatan Antioksidan dan Kesukaan Masyarakat terhadap Teh Daun Gaharu (Aquilaria Malaccensis Lamk) Berdasarkan Pohon Induksi dan Non-Induksi. Peronema Forestry Science Journal, 4(1), 1–12.

Nofita, S. D., Ngibad, K., & Rodli, A. F. (2022). Determination of Percentage ield and Total Phenolic Content of Ethanol Extract from Purple Passion (Passiflora edulis f. edulis Sims) Fruit Peel. Jurnal Pijar Mipa, 17(3), 309–313. https://doi.org/10.29303/jpm.v17i3.3461

Nugroho, A. E., Malik, A., & Pramono, S. (2013). Total Phenolic and Flavonoid Contents, and In Vitro Antihypertension Activity of Purified Extract of Indonesian Cashew Leaves (Anacardium occidentale L.). International Food Research Journal, 20(1), 299–305.

PERDOSKI. (2017). Panduan Praktik Klinis Bagi Dokter Spesialis Kulit dan Kelamin di Indonesia. Perhimpunan Dokter Spesialis Kulit dan Kelamin Indonesia.

Perveen, S., & Al-Taweel, A. (2019). Pharmacognosy: Medicinal Plants. IntechOpen.

PIONAS. (2015). Sediaan Topikal untuk Akne. Pusat Informasi Obat Nasional. https://pionas.pom.go.id/ioni/bab-13-kulit/136-akne-dan-rosasea/1361-sediaan-topikal-untuk-akne

Prananta, Y. E., Rakhman, K. A., & Saleh, J. (2020). Antioxidant Activities of Red Jabon (Anthocephalus macrophyllus) Ethanol Extract. IOP Conference Series: Earth and Environmental Science, 415(1), 1–8. https://doi.org/10.1088/1755-1315/415/1/012026

Pusat Studi Biofarmaka IPB. (2014). Sehat Alami dengan Herbal: 250 Tanaman Berkhasiat Obat. PT. Gramedia Pustaka Utama.

Saifullah, M., McCullum, R., McCluskey, A., & Vuong, Q. (2020). Comparison of Conventional Extraction Technique with Ultrasound Assisted Extraction on Recovery of Phenolic Compounds from Lemon Scented Tea Tree (Leptospermum petersonii) Leaves. Heliyon, 6(4), 1–12. https://doi.org/10.1016/j.heliyon.2020.e03666

Salamah, N., Ahda, M., Bimantara, S., & Hanar, R. (2017). Total Phenolic Content and In Vitro Evaluation of Antioxidant Activity of Ethanol Extract of Ganoderma amboinense. National Journal of Physiology, Pharmacy and Pharmacology, 8(1), 97–101. https://doi.org/10.5455/njppp.2018.8.0518614102017

Sari, L. P. I. P. (2020). Optimasi Pelarut untuk Ekstraksi Daun Nagasari (Mesua ferrea L.) sebagai Anti Radikal Bebas dengan Metode Simplex Lattice Design [Skripsi]. Universitas Mataram.

Shankar, R. (2009). Process Improvement Using Six Sigma: A DMAIC Guide. Quality Press.

Srivastava, A. K., Kannaujiya, V. K., Singh, R. K., & Singh, D. (2021). Evolutionary Diversity as a Source for Anticancer Molecules. Elsevier.

Susiloningrum, D., & Sari, D. E. M. (2021). Uji Aktivitas Antioksidan dan Penetapan Kadar Flavonoid Total Ekstrak Temu Mangga (Curcuma mangga Valeton & Zijp) dengan Variasi Konsentrasi Pelarut. Cendekia Journal of Pharmacy, 5(2), 117–127.

Tukiran, Wardana, A. P., Hidayati, N., & Shimizu, K. (2018). An Ellagic Acid Derivative and Its Antioxidant Activity of Chloroform Extract of Stem Bark of Syzygium polycephalum Miq. (Myrtaceae). Indonesian Journal of Chemistry, 18(1), 26–34. https://doi.org/10.22146/ijc.25467

Utami, S. B. (2020). Aktivitas Penghambatan Radikal Bebasdari Rebusan Daun Renggak (Amomum dealbatum Roxb.) Menggunakan Metode DPPH [Skripsi]. Universitas Mataram.

Wardoyo, E. R. P., Hildayati, U., Rachmawati, & Kurniatuhadi, R. (2021). Phytochemical Analysis and Antifungi Activity of Methanol Extract of Acalypha hispida Burm. F. Flower Against to Candida albicans (Y116). Journal of Physics: Conference Series, 1940(1), 1–7. https://doi.org/10.1088/1742-6596/1940/1/012056

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Anti-Radical Activity Test of Fractions from Ashitaba Herbs (Angelica keiskei)

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