Antioxidant and Antibacterial Activity of Ethanol Extract of Butterfly Leaf (Bauhinia Purpurea L.) with Maceration Extraction Method

Authors

Yasmeen Nazhifah Amani Surendro , Muhammad Amin Nasution , Minda Sari Lubis , Anili Fithri Pulungan

DOI:

10.29303/jbt.v24i4.7963

Published:

2024-11-26

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Abstract

The butterfly plant (Bauhinia purpurea L.) is a plant widely used in traditional medicine to treat various ailments. The butterfly plant's leaves have medicinal properties. The leaves of butterfly plants contain bioactive chemicals that can be employed as natural antibacterial and antioxidant agents. The purpose of this study was to evaluate the ethanol extract of butterfly leaves' antibacterial and antioxidant properties. Butterfly leaf extract was obtained using a maceration process with 96% ethanol solvent. Antioxidant activity was measured using the DPPH (1,1-diphenyl-2-picryhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) techniques, with ascorbic acid as a comparator. The disc paper diffusion method was used to test the antibacterial activity against Streptococcus mutans bacteria. The DPPH method has an IC50 value of 27.5484 µg/ml with a very strong category, while the FRAP method has an IC50 value of 72.9311 µg/ml with a strong category. Butterfly leaf extract showed antibacterial efficacy against Streptococcus mutans bacteria at all doses, with maximum inhibition seen at an extract concentration of 50% (inhibition zone diameter: 16.3 mm ± 0.81). The study's findings suggest that butterfly leaf extract works well as a natural antibacterial and antioxidant.

Keywords:

Antioxidant, antibacterial, Bauhinia purpurea L., maceration, Streptococcus mutans.

References

Aisyah, I. S., Kamaruddin, I., Siburian, U. D., Wahyuni, L. E. T., Amanda, E., Agustina, M., Astuti, I. D., Rahmawati, R., & Kartikasari, M. N. D. (2022). Gizi Kesehatan (M. Sari & R. M. Sahara, Eds.). Global Eksekutif Teknologi.

Aye, M. M. (2021). Screening on Some Bioactivities from The Leaf of Bauhinia purpurea L. (Swedaw-Ni). Journal of the Myanmar Academy of Arts and Science, 19(1A), 32–34.

Chavan, U. D. (2018). Phenolic: Antioxidants and Health Benefits. Scientific Publishers.

Ditjen POM. (1979). Farmakope Indonesia Edisi III. Departemen Kesehatan RI.

Ditjen POM. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jendral Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional.

Htay, T. M., Sann, K. K., & Haini, H. (2023). Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Bauhinia purpurea. Bioactivities, 1(1), 24–29. https://doi.org/10.47352/bioactivities.2963-654X.183ï

Isnindar. (2021). Senyawa Antioksidan Daun Kesemek (Diospyros kaki Thunb) dengan Metode DPPH (Safrinal, Ed.). CV. Azka Pustaka.

Karyati, & Adhi, M. A. (2018). Jenis-jenis Tumbuhan Bawah di Hutan Pendidikan Fakultas Kehutanan Universitas Mulawarman (D. E. Bulan, Ed.). Mulawarman University Press.

Kurniasari, Y., Khasanah, K., Yunita, V., Alawiyah, L., & Wijayanti, P. (2022). Aktivitas Antioksidan Ekstrak Serbuk Bekatul Menggunakan Metode DPPH, ABTS, dan FRAP. CERATA Jurnal Ilmu Farmasi, 13(2), 28–30. https://doi.org/10.61902/cerata.v13i2.612

Sambodo, D. K., Marsel, F., Sambodo, H. P., & Arlesia, N. (2022). Pengaruh Perbedaan Metode Ekstraksi Daun Jati (Tectona grandis L.f) Terhadap Aktivitas Antibakteri pada Escherichia coli. Jurnal Riset Kefarmasian Indonesia, 4(2), 160–163. https://doi.org/10.33759/jrki.v4i2.259

Shajiselvin, C. D., Somasundaran, G., & Muthu, A. K. (2011). Antioxidant Capacity of Various Extracts from Whole Plant of Bauhinia Purpurea (Linn) Evaluated by Three In Vitro Methods. Pharmacologyonline, 1(1), 223226.

Suryani, N. P. F., Hita, I. P. G. A. P., & Septiari, I. G. A. A. (2023). Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Daun Porang (Amorphophallus muelleri B.) dengan Pelarut Ekstraksi Etanol, Etil Asetat dan N-Heksana. Journal Scientific of Mandalika (JSM), 4(9), 187189. https://doi.org/10.36312/10.36312/vol4iss9pp179-194

Sutrisna, E. (2016). Herbal Medicine: Suatu Tujuan Farmakologis. Muhammadiyah University Press.

Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Uji Aktivitas Antimikroba dari Ekstrak dan Fraksi Ascidian herdmania momus dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium dan Candida albicans. Pharmacon, 10(1), 706. https://doi.org/10.35799/pha.10.2021.32758.

Widyaningsih, T. D., Wijayanti, N., & Nugrahini, N. I. P. (2017). Pangan Fungsional: Aspek Kesehatan, Evaluasi, dan Regulasi. Universitas Brawijaya Press.

Author Biographies

Yasmeen Nazhifah Amani Surendro, Universitas Negeri Medan

Author Origin : Indonesia

Minda Sari Lubis, Universitas Muslim Nusantara (UMN) Al-Washliyah

Author Origin : Indonesia

Anili Fithri Pulungan, Universitas Muslim Nusantara (UMN) Al-Washliyah

Author Origin : Indonesia

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How to Cite

Surendro, Y. N. A., Nasution , M. A., Lubis, M. S., & Pulungan, A. F. (2024). Antioxidant and Antibacterial Activity of Ethanol Extract of Butterfly Leaf (Bauhinia Purpurea L.) with Maceration Extraction Method. Jurnal Biologi Tropis, 24(4), 1040–1049. https://doi.org/10.29303/jbt.v24i4.7963

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