Isolation and Identification of Bacteria in the Integrated Laboratory of the Faculty of Medicine, Moslem Indonesia of University
DOI:
10.29303/jbt.v24i1b.8219Published:
2024-12-14Downloads
Abstract
The integrated laboratory is one of the learning facilities at the university, especially to support the implementation of practicum. In laboratories that are full of activities, bacterial contamination often occurs which can interfere with the smooth running of research and practicum. This contamination is often caused by a lack of sterility in the work process and less than optimal indoor air quality. Therefore, it is important to know the type and number of bacteria found in the laboratory to prevent further spread of contamination. This study aims to isolate and identify bacteria in the Integrated Laboratory of the Faculty of Medicine, Universitas Muslim Indonesia. The method used in this study was descriptive research with a laboratory observational design, which was carried out by macroscopic and microscopic means to determine the type of microorganisms and the number of bacterial colonies. The results showed four types of bacteria found, namely Staphylococcus sp., Klebsiella pneumoniae, Staphylococcus epidermidis, and Bacillus sp., which were isolated from air and swab results. Airborne bacteria had the highest number of colonies at 14 CFU, while swab bacteria were found at 200 CFU. Although bacterial colonies were found in the laboratory, this number is still considered safe for the number of bacteria in the laboratory. Based on these findings, it is recommended that laboratory facilities pay more attention to aspects of cleanliness and sanitation as a whole.
Keywords:
Bacterial, isolation, identification, integrated laboratory.References
A’yun, Q., Asmarany R, A., Fitriyah, D., Awaluddin, Rini, I. A., Mahyarudin, Argaheni, N. B., Sinaga, J., Suryanti, E., Kristianto, Y., Asril, M., & Hamida, F. (2022). Mikrobiologi Dasar. In R. Watrianthos (Ed.), Angewandte Chemie International Edition, 6(11), 951–952. (Vol. 3, Issue 1). Yayasan Kita Menulis. Https://Medium.Com/@Arifwicaksanaa/Pengertian-Use-Case-A7e576e1b6bf
Ainun, V., Erni, Y., & Avidhatul, Y. (2024). Identifikasi Bakteri Patogen Gram Negatif Pada Alat Praktikum Mikrobiologi Di Laboratorium Central Stikes Maharani Malang. Journal Borneo, 4(2), 67–80. Https://Doi.Org/10.57174/J.Born.V4i2.144
Andriana, Y., Widodo, A. D. W., & Endraswari, P. D. (2023). A Correlation Between The Number Of Airborne Bacteria And Fungi Using The Settle Plate Method With Temperature And Relative Humidity At The Clinical Microbiology Laboratory Of Dr. Soetomo Regional General Hospital Surabaya, Indonesia. Journal Of Pure And Applied Microbiology, 17(2), 942–950. Https://Doi.Org/10.22207/Jpam.17.2.24
Atirah, N., & Liana, D. F. (2023). Gambaran Angka Bakteri Di Laboratorium Mikroskopik Fakultas Kedokteran Universitas Tanjungpura. Majalah Kedokteran Andalas, 46(2), 282–294. Https://Doi.Org/Https://Doi.Org/10.25077/Mka.V46.I2.P282-294.2023
Dewi, W. C., Raharjo, M., & Wahyuningsih, N. E. (2021). Literatur Review : Hubungan Antara Kualitas Udara Ruang Dengan Gangguan Kesehatan Pada Pekerja. An-Nadaa: Jurnal Kesehatan Masyarakat, 8(1), 88. Https://Doi.Org/10.31602/Ann.V8i1.4815
Hidayat, S. M., Hakim, R., & Airlangga, H. (2019). Hubungan Antara Jumlah Koloni Bakteri Pada Gagang Dan Handle Pintu Troli Gizi Dengan Jumlah Ruangan Pada Rotasi Malam Troli Gizi Di Sebuah Rumah Sakit Kota Malang. Jurnal Bio Komplementer Medicine, 1, 1–8. Https://Jim.Unisma.Ac.Id/Index.Php/Jbm/Article/View/2014/1934
Nasution, G. S., & Lubis, A. (2022). Gambaran Keberadaan Bakteri Bacillus Sp. Pada Ruangan Ber-Ac Dan Non Ac Systematic Review. Sains Medisina, 1(2), 130–140. Https://Wpcpublisher.Com/Jurnal/Index.Php/Sainsmedisina/Article/View/148/97
Permenkes. (2019). Peraturan Menteri Kesehatan Republik Indonesia Nomor 7 Tahun 2019 Tentang Kesehatan Lingkungan Rumah Sakit. In Menteri Kesehatan Republik Indonesia. Https://Doi.Org/.1037//0033-2909.I26.1.78
Sari, A., & Soleha, T. (2020). Kualitas Mikrobiologi Udara Dan Identifikasi Jenis Mikroorganisme Pada Lantai Ruang Intensive Care Unit (Icu) Di Rumah Sakit Umum Daerah Dr. H. Abdoel Moeloek Bandar Lampung. Medula, 10(3). Https://Doi.Org/Https://Doi.Org/10.53089/Medula.V10i3.103
Unair News. (2023). Dampak Pencemaran Mikroba Di Udara Bagi Kesehatan Tubuh. Universitas Airlangga. Https://Unair.Ac.Id/Dampak-Pencemaran-Mikroba-Di-Udara-Bagi-Kesehatan-Tubuh/
Wahyuni, R. D. (2017). Identifikasi Bakteri Udara Pada Instalasi Radiologi Rumah Sakit Umum Daerah Undata Palu. Jurnal Kesehatan Tadulako, 3(1), 36–42. Https://Doi.Org/Https://Doi.Org/10.22487/Htj.V3i1.37
Wismana, W. S. (2019). Gambaran Kualitas Mikrobiologi Udara Kamar Operasi Dan Keluhan Kesehatan. Jurnal Kesehatan Lingkungan, 6(2), 219–226. Https://E- Journal.Unair.Ac.Id/Jkl/Article/Download/8015/474
License
Copyright (c) 2024 Marzelina Karim, Dhian Karina Aprilani Hattah, Haslianti H. Haris, Churiani Churiani
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
