Vol. 25 No. 2 (2025): April-Juni
Open Access
Peer Reviewed

Optimizing Bioelectricity Production from Thermophilic Bioelectrogens Consortium Using Agricultural Waste in Microbial Fuel Cells

Authors

Apriansa Apriansa , Irdawati Irdawati

DOI:

10.29303/jbt.v25i2.8853

Published:

2025-05-20

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Abstract

Significant economic and population growth around the world has led to various problems, especially fossil fuel scarcity, energy production, as well as an increase in the volume of organic waste (agricultural, municipal, and industrial waste). As an alternative energy source, Microbial Fuel Cell (MFC) was chosen due to its promising prospects. The use of thermophilic bacteria and consortiums were chosen for their potential advantages in MFC systems. This study aims to explore the potential of thermophilic bioelectrogenous bacterial isolates of Sungai Sapan Aro (SSA) consortium 14&16 in producing bioenergy using various agricultural waste substrates (corn cob, rice straw, rice husk, and glucose as control). The results showed no significant difference in the use of agricultural waste substrates in the MFC system. Quantitatively, corn cobs produced voltages almost equivalent to glucose (control), while rice straw and rice husk produced lower voltages. The resulting voltages were glucose (0.59467 V), corn cob (0.57633 V), rice straw (0.43300 V), and rice husk (0.40400 V). The results of this study show better performance compared to previous studies in the field of electricity generation through MFCs.

Keywords:

consortium; microbial fuel cell; thermophilic bioelectrigens; waste agricultural substrates

References

Arigeni, R., Kirom, M. R., & Qurthobi, A. (2019). Analisis Produksi Energi Listrik Pada Microbial Fuel Cell Menggunakan Substrat Tongkol Jagung Dengan Kontrol Suhu. eProceedings of Engineering, 6(1): 1091-1096. https://openlibrarypublications.telkomuniversity.ac.id/index.php/engineering/article/view/8883/0

Barua, P. K., & Deka, D. (2010). Electricity Generation from Biowaste Based Microbial Fuel Cell. International Journal of Energy, Information, and Communications, 1(1): 77-92. https://www.researchgate.net/publication/281767156_Electricity_generation_from_biowaste_based_microbial_fuel_cells

Bazargan, A., Wang, Z., Barford, J. P., Saleem, J., & McKay, G. (2020). Optimization of the Removal of Lignin and Silica from Rice Husks with Alkaline Peroxide. Journal of Cleaner Production, 260. 120848. https://doi.org/10.1016/j.jclepro2020.120848

Bond, D. R. & Lovley, D. R. (2003). Electricity Production by Geobacter sulfurreducens Attached to Electrodes. Appl Environ Microbiology 69(3):1548-1555. DOI: 10.1128/AEM.69.3.1548-1555.2003

Cao, L., Sun., H., Ma, Y., Lu, M., Zhao, M., Li, E., & Liu, Y. (2023). Analysis and Enhancement of the Energy Utulization Efficiency of Corn Stover using Strain Lsc-8 in a Bioelectrochemical System. Microbial Cell Factories, 22 (54). https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02058-6

Chae, K. J., Choi, M. J., Lee, J. W., Kim, K. Y & Kim, I. S. (2009). Effect of Different Substrates on Performance, Bacterial Diversity, and Bacterial Viability in Microbial Fuel Cell. Bioresource Technology, 3518-3525. DOI :10.1016/j.biortech.2009.02.065.

Chandra, R., Takeuchi, H., & Hasegawa, T. (2007). Methane Production from Lignocellulosic Agricultural Crop Wastes: A Eeview in Context to Second-Generation Biofuel Production. Renewable and Sustainable Energy Reviews, 16(3): 1462-1476. https://doi.org/10.1016/j.rser.2011.11.035

Dai, K., Wen, J. L., Zhang, F., Ma, X. W., Cui, X. Y., Zhang, Q., Zhao, T. J., & Zeng, R. J. (2017). Electricity Production and Microbial Characterization of Thermophilic Microbial Fuel Cells. Bioresour Technology, 243:512-519. https://doi.org/10.1016/j.biortech.2017.06.167

Flores, S. R. (2022). Generation of Electricity from Agricultural Waste. Green Energy and Environmental Technology: 1–6. DOI: https://doi.rg/10.5772/geet.11

Fu, Q., Fukushima, N., Maeda, H., Sato, K., & Kobayashi, H. (2015). Analisis Bioelektrokimia Sel Bahan Bakar Mikroba Hipertermofilik yang Menghasilkan Listrik pada Suhu di atas 800 C. Biosains, Bioteknologi, dan Biokimia, 79 (7), 1200-1206. https://doi.org/10.1080/09168451.2015.1015952

Goyal, S. & Sindhu, S. S. (2011). Composting of Rice Straw Using Different Inocula and Analysis of Compost Quality. Microbiolgy Journal, 1(4):126-138. DOI:10.3923/mj.2011.126.138.https://scialert.net/abstract/?doi=mj.2011.126.138

Hassan, S. H. A., Sanaa, M. F., Rab, G. E., Rahimnejad, M., Ghasemi, M., Joo, J.H., Ok, Y. S., Kim, I. S., & Oh, S.E. (2014). Electricity Generation from Rice Straw using a Microbial Fuel Cell. International Journal of Hydrogen Energy XXX, 1-7. http://dx.doi.org/10.1016/j.ijhydene.2014.03.259.

Hendriks, A. T. W. M., & Zeeman, G. (2009). Pretreatments to Enhance the Digestibility of Lignocellulosic Biomass. Bioresource Technology, 100(1), 10-18. https://doi.org/10.1016/j.biortech.2008.05.027

Huang, L., Zeng R. J., & Angelidaki I. (2008). Electricity Production from Xylose using a Mediator-less Microbial Fuel Cell. Bioresour Technol, 99(10):4178-4184. https://doi.org/10.1016/j.biortech.2007.08.067

Irdawati, I., Auliya, P. R, Putri, D. H, Handayani, D., & Yusrizal, Y. (2023). Kemampuan Konsorsium Trikultur Bakteri Termofilik dari Sumber Air Panas Mudiak Sapan untuk Menghasilkan Biofuel. Jurnal Penelitian Pendidikan IPA, 9 (4): 2265-2270. DOI: 10.29303/jppipa.v9i4.3480

Irdawati, I., Sofiyyana, A., Advinda, L., Fiffendy, M., Syamsuardi, S., Agustien, A., Rilda, Y., & Yahya, Y. (2020). Optimizing of Agricultural Waste Substrate as an Alternative Medium for Xylan in Producing Xylanase Enzymes by Thermophilic Bacteria. Journal of Physics: Conference Series 1940 012052. DOI: 10.1088/1742-6596/1940/1/012052.

Karim, I., Syahruddin., & Bahri, S. (2022). Kandungan Selulosa, Hemiselulosa, dan Lignin Jerami Padi yang Difermentasi dengan Berbagai Probiotik. Jambura Journal Animal Science, 6(1): 13-21. DOI:https://doi.org/10.35900/jjas.v6i1.19241

Kaur, K., & Phutela, U. G. (2016). Enhancement of Paddy Straw Digestibility and Biogas Production by Sodium Hydroxide-microwave Pretreatment. Renew. Energy 92, 178-184.https://doi.org/10.1016/j.renene.2016.01.083

Khoirunnisa, N. S., Santosa, D. A., & Syaiful, A. (2020). Performa Microbial Fuel Cell dengan Substrat Jerami Padi Hasil Pra-Perlakuan NaOH-Gelombang Mikro dan Inokulasi Bakteri Selulotik. Thesis. Institut Pertanian Bogor. Jawa Barat, Indonesia. https://repository.ipb.ac.id/handle/123456789/105198

Khaleghian, H., Molaverdi, M., & Karimi, K. (2017). Silica Removal from Rice Straw to Improve its Hydrolysis and Ethanol Production, Indsutrial and Engineering Chemistry Research, 56(35). DOI:10.1021/acs.iecr.7b02830.

Khudzari, J. M., Tartakovsky, B. & Raghavan, G. S. V. (2016). Effect of C/N Ratio and Salinity on Power Generation in Compost Microbial Fuel Cells. Waste Management, 48 :135-142. https://doi.org/10.1016/j.wasman.2015.11.022

Krishania, M., Kumar, V., Vijay, V. K., & Malik, A., (2013). Analysis of different techniques used for improvement of biomethanation process: a review. Fuel 106, 1-9. https://doi.org/10.1016/j.fuel.2012.12.007

Liu, Z., Xu, A., & Long, B. (2011). Energy from combustion of rice straw: status and challenges to China. Energy Power Eng. 03, 325-331. https://doi.org/10.4236/epe.2011.33040

Li, M., Zhou, M., Tian, X., Tan, C., McDaniel, C. T., Hassett D. J., & Gu, T. (2018). Microbial Fuel Cell (MFC) Power Performance Improvement through Enhanced Microbial Electrogenicity. Biotechnol Adv. 36(4):1316-1327. https://doi.org/10.1016/j.biotechadv.2018.04.010

Lynd, L. R., Weimer, P. J., Zyl, V. W. H., & Pretorius, I. S. (2002). Microbial Cellulose Utilization: Fundamentals and Biotechnology. Microbiology and Molecular Biology Reviews, 66(3): 506-577. doi: 10.1128/MMBR.66.3.506-577.2002.

Ma’Ruf, A., Pramudono, B., & Aryanti, N. (2017). Lignin Isolation Process from Rice Husk by Alkaline Hydrogen Peroxide: Lignin and Silica Extracted. AIP Conference Proceedings, 1823. https://doi.org/10.1063/1.4978086

Matin, H. H. A. & Hadiyanto, H. (2018). Produksi Biogas dari Limbah Sekam Padi dengan Metode State Anaerobic Digestion (SSAD). E3S Web of Conference 31, 02007. https://doi.org/10.1051/e3sconf/20183102007

Mohammadi, F., Nezhaad, G. R. V., Rahawi, N. A., & Gholipour, S. (2023). Microbial Electrochemical Systems for Bioelectricity Generation: Current State and Future Directions. Results in Engineering20.https://doi.org/10.1016/j.rineng.2023.101619

Novia., Wijaya, D. & Yanti, P. (2017). Pengaruh Waktu Delinifikasi terhadap Lignin dan Waktu SSF Terhadap Etanol Pembuatan Bioetanol dari Sekam Padi. Jurnal Teknik Kimia, 23(1): 19-27. https://garuda.kemdikbud.go.id/documents/detail/2396628

Nugroho, I., Amaliyah, R. I. U., & Kirom, M. R. (2020). Pengaruh Lama Pembusukan Nasi Basi dan Rasio Volume Variasi Substrat Terhadap Produksi Energi Listrik pada Sistem MFC. e-Proceeding of Engineering. 7(1): 1230-1236. https://www.academia.edu/79317999/Pengaruh_Lama_Pembusukan_Nasi_Basi_Dan_Rasio_Volume_Variasi_Substrat_Terhadap_Produksi_Energi_Listrik_Pada_Sistem_MFC

Prakoso, H. T., Widiastuti, H., Suharyanto., & Siswanto. (2014). Eksplorasi dan Karakterisasi Bakteri Aerob Ligninolitik serta Aplikasinya untuk Pengomposan Tandan Kosong Kelapa Sawit. Menara Perkebunan, 82(1): 15-24.https://doi.org/10.22302/iribb.jur.mp.v82i1.27

Pratiwi, R., Rahayu, D. & Barliana, M. I. (2016). Pemanfaatan Selulosa dari Limbah Jerami Padi (Oryza sativa) sebagai Bahan Bioplastik. Jurnal Sains dan Teknologi Farmasi Indonesia, 3(3):83-91. DOI: https://doi.org/10.15416/ijpst.v3i3.9406

Rabaey, K., & Verstraete, W. (2005). Microbial Fuel Cells: Novel Biotechnology for Energy Generation. Trends in Biotechnology, 23(6): 291-298. https://doi.org/10.1016/j.tibtech.2005.04.008

Savvidou, M. G., Pandis, P. K., Mamma, D., Sourkouni, G., & Argirusis, C. (2022). Organic Waste Substrates for Bioenergy Production via Microbial Fuel Cells: A Key Point Review. Energies 15(15): 5616. https://doi.org/10.3390/en15155616

Septiningrum, K., Apriana, & Chandra. (2011). Produksi Xilanase dari Tongkol Jagung dengan Sistem Bioproses menggunakan Bacillus circulans untuk Pra-pemutihan Pulp. Journal of Industrial Research (Jurnal Riset Industri). 5(1): 87-89. https://www.neliti.com/id/publications/71909/produksi-xilanase-dari-tongkol-jagung-dengan-sistem-bioproses-menggunakan-bacill

Singh, R. & Kumar, S. (2019). A Review on Biomethane Potential of paddy straw and Diverse Prospects to Enhance its Biodigestibility. Journal of Cleaner Production, 217: 295-307. https://doi.org/10.1016/j.jclepro.2019.01.207

Suherman, D., Irdawati, & Andrian. 2024. Growth Pattern of Thermophilic Bacterial Isolate SSA-16 from Sapan Sungai Aro Hot Springs. Serambi Biologi, 9(2): 193-198. https://doi.org/10.24036/srmb.v9i2.350

Sulistiyawati, I., Rahayu, N. L., & Purwitaningrum, F. S. (2020). Produksi Biolistrik Menggunakan Microbial Fuel Cell (MFC) Lactobacillus bulgaricus dengan Substrat Limbah Tempe dan Tahu. Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal, 37(2): 112-117. https://www.academia.edu/90286483/Produksi_Biolistrik_menggunakan_Microbial_Fuel_Cell_MFC_Lactobacillus_bulgaricus_dengan_Substrat_Limbah_Tempe_dan_Tahu

Surra, E., Bernardo, M., Lapa, N., Esteves, I. A. A. C., Fonseca, I. & Mota, J. P. B. (2019). Biomethane Production Through Anaerobic Co-digestion with Maize Cob Waste Based on a Biorefinery Concept : A Review. Journal of Environmental Management, 249 109351. https://doi.org/10.1016/j.jenvman.2019.109351

Tariq, M., Wang, J., Malik, A. J., Akhter, M. S. & Mahmood, Q. (2021). Effecr of Substrate Ratios on the Simultaneous Carbon, Nitrogen, Sulfur, and Phosphorous Conversions in Microbial Fuel Cells. Heliyon, 7(6). https://doi.org/10.1016/j.heliyon.2021.e07338

Thiyageshwari, S., Gayathri, P., Krishnamoorthy, R., Anandham, R. & Paul, D. (2018). Exploration of Rice Husk Compost as an Alternate Organic Manure to Enhance the Productivity of Blackgram in Typic Haplustalf and Typic Rhodulstalf. International Journal of Environmental Research and Public Health, 15(2):358. DOI: 10.3390/ijerph15020358

Utomo, Y. & Fadila, E. N. (2020). Isolasi Lignin dari Sekam Padi (Oriza sativa L.) serta Pemanfaatannya sebagi Adsorben Ion Cd(II). Journal Cis-Trans, 4(2): 19-26. DOI: 10.17977/um0260v4i22020p019.

Veluchamy, C. & Kalamdhad, A. S. (2017). Influence of Pretreatment Techniques on Anaerobic Digestion of Pulp and Paper Mill Sludge : A Review. Bioresource Technology, 245 : 1206-1219.https://doi.org/10.1016/j.biortech.2017.08.179

Verma, J., Kumar, D., Singh, N., Katti, S. S., & Shah, Y. T. (2021). Electricigens and Microbial Fuel Cells for Bioremediation and Bioenergy Production: A Review. Environmental Chemistry Letters. 19 : 2091-2126. https://link.springer.com/article/10.1007/s10311-021-01199-7

Verma, P., Daverey, A., Kumar, A., & Arunachalam, K. (2021). Microbial Fuel Cell–A Sustainable Approach for Simultaneous Wastewater Treatment and Energy Recovery. Journal of Water Process Engineering 40, 101768. https://doi.org/10.1016/j.jwpe.2020.101768

Vestimarta, A. W. & Irdawati. (2024). Produksi Biolistrik dengan Microbial Fuel Cell (MFC) dari Bakteri Termofilik. Jurnal Pendidikan dan Sains. 4(1): 359-366. DOI: 10.58578/masaliq.v4i1.2632

Vestimarta, A. W., & Irdawati. (2024). Profil Kurva Pertumbuhan Bakteri Termofilik Isolat SSA-8 dari Sumber Air Panas Sapan Sungai Aro. Jurnal Pendidikan Tambusai, 8 (1) : 15802-15808. https://doi.org/10.31004/jptam.v8i1.14597

Vestimarta, A. W. (2024). Potensi Konsorsium Bikultur Bakteri Termofilik Electrigens sebagai Microbial Fuel Cell (MFC) dalam Menghasilkan Energi Listrik. Skripsi. Padang ID : Universitas Negeri Padang, Indonesia.

Wang, M.C., Liu, T.T., Zhang, X.J., Wu, D. & F, L.P. (2018). Effect of Anode Substrate on the Performance of Microbial Fuel Cells for Dealing with the Straw Hydrolysate. Journal of Fuel Chemistry and Technology. 46(6). https://www.researchgate.net/publication/327988887_Effect_of_anode_substrate_on_the_performance_of_microbial_fuel_cells_for_dealing_with_the_straw_hydrolysate

Wijaksono, T., Rakhmawati, A. & Arnandha, Y. (2021). Sekam Padi dan Batang Bambu sebagai Bahan Balok Komposit dengan menggunakan Matriks Resin Polyester. Jurnal Reviews in Civil Engineering, 5(2): 83-86. DOI:10.31002/rice.v5i2.4813

Yadav, M., Sehrawat, N., Singh, M., Kumar, V., Sharma, A. K., & Kumar, S. (2021). Thermophilic Microbes-based Fuel Cells: an Eco-friendly Approach for Sustainable Energy Production. Bioremediation for Environmental Sustainability. pp. 235-246. https://doi.org/10.1016/B978-0-12-820318-7.00011-3

Zhang, Y., Fan., S., Liu, T., Omar, M. M. & Li, B. (2022). Perspectives into Intensification for Aviation Oil Production from Microwave Pyrolysis of Organic Wastes. Chemical Engineering and Processing. 176 108939. https://doi.org/10.1016/j.cep.2022.108939.

Zulfikar, E. S., Tamjidillah, M., & Ramadhan, M. N. (2021). Produktivitas Listrik Microbial Fuel Cell pada Substrat Limbah Air Rebusan Mie Instan. Rotary, 3(1): 69-80. https://scholar.google.co.id/citations?view_op=view_citation&hl=id&user=vC5nX4kAAAAJ&citation_for_view=vC5nX4kAAAAJ:maZDTaKrznsC

Author Biographies

Apriansa Apriansa, Universitas Negeri Padang

Author Origin : Indonesia

Irdawati Irdawati, Universitas Negeri Padang

Author Origin : Indonesia

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How to Cite

Apriansa, A., & Irdawati, I. (2025). Optimizing Bioelectricity Production from Thermophilic Bioelectrogens Consortium Using Agricultural Waste in Microbial Fuel Cells. Jurnal Biologi Tropis, 25(2), 1853–1863. https://doi.org/10.29303/jbt.v25i2.8853

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