Vol. 25 No. 2 (2025): April-Juni
Open Access
Peer Reviewed

Hydrolysed Fish Peptide, Turmeric and Garlic Powder Supplementation to Reducing Vibrio Bacteria in Litopenaeus vannamei

Authors

Lilik Setiyaningsih , Ren Fitriadi , Baruna Kusuma , Jefri Anjaini , Purnama Sukardi

DOI:

10.29303/jbt.v25i2.8958

Published:

2025-05-22

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Abstract

Vibriosis disease caused by Vibrio bacteria is one of the main challenges in vaname shrimp (Litopenaeus vannamei) farming, as it can cause high mortality rates and decreased productivity. This study aims to evaluate the effectiveness of a combination of natural immunostimulants in turmeric (Curcuma longa) and garlic (Allium sativum) combined with hydrolysis of fish peptides in suppressing Vibrio spp. bacterial populations in the hepatopancreas organs. The study was conducted for 30 days using a completely randomised design of four treatments and three replications. Treatment A (feed+turmeric75%+garlic25%+fish peptide hydrolysis2%), Treatment B (feed+turmeric50%+garlic50%+fish peptide hydrolysis2%), Treatment C (feed+turmeric25%+garlic75%+fish peptide hydrolysis2%), and Treatment K (feed without immunostimulant and fish peptide hydrolysis). Total Vibrio spp. bacteria were counted using the total plate count method on the hepatopancreas organ of the shrimp. The results showed that the immunostimulant combination of turmeric 75%, garlic 25%, and fish peptide hydrolysis 2% (treatment A) significantly reduced the total number of Vibrio spp. bacteria in the hepatopancreas to 2.45 ± 0.213 log CFU/ml compared to the control. The combination of turmeric and garlic immunostimulants and fish peptide hydrolysis has the potential to be an effective natural strategy in controlling vibriosis disease in shrimp farming.

Keywords:

Fish peptide hydrolysis, garlic, immunostimulant, Litopenaeus vannamei, turmeric, vibriosis.

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Author Biographies

Lilik Setiyaningsih, universitas jenderal soedirman

Author Origin : Indonesia

Ren Fitriadi, Universitas Jenderal Soedirman

Author Origin : Indonesia

Baruna Kusuma, Universitas Jenderal Soedirman

Author Origin : Indonesia

Jefri Anjaini, Universitas Jenderal Soedirman

Author Origin : Indonesia

Purnama Sukardi, Universitas Jenderal Soedirman

Author Origin : Indonesia

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How to Cite

Setiyaningsih, L., Fitriadi, R., Kusuma, B., Anjaini, J., & Sukardi, P. (2025). Hydrolysed Fish Peptide, Turmeric and Garlic Powder Supplementation to Reducing Vibrio Bacteria in Litopenaeus vannamei. Jurnal Biologi Tropis, 25(2), 1989–1998. https://doi.org/10.29303/jbt.v25i2.8958

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