Vol. 25 No. 2 (2025): April-Juni
Open Access
Peer Reviewed

In Vitro Growth Response of Porang (Amorphophallus muelleri Blume) Bulbil to Kinetin Concentration

Authors

Rut Normasari

DOI:

10.29303/jbt.v25i2.9050

Published:

2025-05-25

Downloads

Abstract

The plant known as porang (Amorphophallus muelleri Blume) has a high glucomannan content in its tubers. The availability of porang seeds is one of the elements influencing porang production, hence tissue culture must be developed to improve seed procurement. For porang seed propagation to be successful in vitro, growth regulators must be added. This study aims to determine the effect of adding kinetin concentration on porang shoot induction. Five treatments the addition of kinetin at doses of 0, 1, 2, 3, and 4 mg/L were included in the Non-Factorial Completely Randomized Design (CRD) experiment. All observation variables, including explant growth, shoot emergence time, percentage of shoots created, total number of shoots, and number of shoots per explant, were found to be significantly impacted by the addition of kinetin. With a shoot emergence time of 21.86 days after inoculation, 96% of the shoots produced, a total number of 108.80 shoots, and a number of shoots of 22.75 per explant, kinetin 4 mg/L was the optimal concentration for porang shoot induction.

Keywords:

Porang, bulbil, kinetin, shoot, in vitro.

References

Abu-Romman, S. M., Al-Hadid, K. A., & Arabiyyat, A. R. (2015). Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber. Journal of Agricultural Science, 7(10), 159–165. https://doi.org/10.5539/jas.v7n10p159

Azizi, I., & Kurniawan, F. (2021). Pengaruh Bibit Asal, Umur, dan Ukuran terhadap Kadar Glukomanan dan Kadar Oksalat dalam Umbi Porang. Jurnal Sains Dan Seni ITS, 9(2). https://doi.org/10.12962/j23373520.v9i2.58571

Barciszewski, J., Siboska, G. E., Pedersen, B. O., Clark, B. F. C., & Rattan, S. I. S. (1997). A Mechanism for the In Vivo Formation of N6-Furfuryladenine, Kinetin, as A Secondary Oxidative Damage Product of DNA. FEBS Letters, 414(2), 457–460. https://doi.org/10.1016/S0014-5793(97)01037-5

Boer, D., Prawansa, A., Rakian, T. C., Arsyad, M. A., Arif, N., Madiki, A., & Arsana, I. M. W. (2024). Induksi Tunas Pisang Kepok (Musa paradisiaca L.) Menggunakan Kombinasi Kinetin dan IAA Secara In Vitro. Jurnal Agroteknos, 14(2), 55–60. https://doi.org/10.56189/ja.v14i2.49085

Ferziana, Erfa, L., Maulida, D., Sari, R. M., & Yuniardi, F. (2021). In Vitro Regeneration of Porang (Amorphophallus muelleri Blume) at Several Concentrations of BAP (Benzyl Amino Purine). International Conference on Agriculture and Applied Science (ICoAAS) 2021, 77–83. https://doi.org/10.25181/icoaas.v2i2.2486

Gantait, S., Kundu, S., & Das, P. K. (2018). Acacia: An Exclusive Survey on In Vitro Propagation. Journal of the Saudi Society of Agricultural Sciences, 17(2), 163–177. https://doi.org/10.1016/j.jssas.2016.03.004

Gubiš, J., Lajchová, Z., Faragó, J., & Jureková, Z. (2003). Effect of Genotype and Explant Type on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro. Czech Journal of Genetics and Plant Breeding, 39(1), 9–14. https://doi.org/10.17221/3715-CJGPB

Haring, F., Farid, M., Ridwan, I., Fadhilah, A. N., & Sedayu, N. (2024). Concentration of 2,4-D and BAP Combination on Callus Induction of Porang Plant (Amorphophallus muelleri Blume). Asian Journal of Plant Sciences, 23(3), 313–320. https://doi.org/10.3923/ajps.2024.313.320

Hidayah, N., Suhartanto, M. R., & Santosa, E. (2018). Pertumbuhan dan Produksi Benih Iles-iles (Amorphophallus muelleri Blume) Asal Teknik Budi Daya yang Berbeda. Buletin Agrohorti, 6(3), 386. https://doi.org/10.29244/agrob.6.3.386-392

Hosiana, A. M., Pinatih, G. N. I., & Laksemi, D. A. A. S. (2023). Beneficial Health Effects of Porang (Amorphophallus muelleri): A Review. Indonesia Journal of Biomedical Science, 17(2), 235–238. https://doi.org/10.15562/ijbs.v17i2.484

Ibrahim, M. S. D. (2019). Perbanyakan Iles-iles (Amorphophallus spp.) secara Konvensional dan Kultur In Vitro serta Strategi Pengembangannya. Perspektif, 18(1), 67. https://doi.org/10.21082/psp.v18n1.2019.67-78

Ibrahim, M. S. D., Sulistiyorini, I., & Tresniawati, C. (2022). Effect of 6-Benzyl Amino Purine on the Multiplication Ability of Shoots of Various Sizes of Porang (Amorphophallus muelleri Blume) Bulbils. IOP Conference Series: Earth and Environmental Science, 974(1), 012091. https://doi.org/10.1088/1755-1315/974/1/012091

Ibrahim, M. S. D., Sulistiyorini, I., Tresniawati, C., Izzah, N. K., & Hartati, Rr. S. (2024). Evaluation of Germination and Multiplication of Micro Shoot of Porang (Amorphophallus muelleri Blume) In Vitro. AIP Conference Proceedings 2957, 040033. https://doi.org/10.1063/5.0184596

Ikayanti, F., Radian, & Rianto, F. (2021). Pertumbuhan dan Hasil Tanaman Porang Periode Pertanian Pertama pada Tanah Gambut dengan Pemberian Pupuk NPK. Jurnal Pertanian Agros, 23(2), 319–326. https://doi.org/10.37159/jpa.v25i4.3528

Kumar, S., Chandra, A., & Gupta, M. G. (2008). Plantlet Regeneration via Multiple Shoot Induction in Indian Cultivars of Lucerne (Medicago sativa L). Journal of Plant Biochemistry and Biotechnology, 17(2), 181–184. https://doi.org/10.1007/BF03263282

Kumar, S., Singh, R., Kalia, S., Sharma, S., & Kalia, A. K. (2016). Recent Advances in Understanding the Role of Growth Regulators in Plant Growth and Development in Vitro-I. Conventional Growth Regulators. Indian Forester, 142(5), 459–470. https://doi.org/10.36808/if%2F2016%2Fv142i5%2F95052

Magyar-Tábori, K., Dobránszki, J., Teixeira da Silva, J. A., Bulley, S. M., & Hudák, I. (2010). The Role of Cytokinins in Shoot Organogenesis in Apple. Plant Cell, Tissue and Organ Culture (PCTOC), 101(3), 251–267. https://doi.org/10.1007/s11240-010-9696-6

Nugroho, K., Kosmiatin, M., Santoso, T. J., Sukma, D., Purwito, A., Husni, A., & Martasari, C. (2024). Optimization of Kinetin Concentrations and Medium Compositions for Citrus Shoot Multiplication from Cotiledonary Node. BIOTROPIA, 31(1), 96–105. https://doi.org/10.11598/btb.2024.31.1.2136

Pasternak, T. P., & Steinmacher, D. (2024). Plant Growth Regulation in Cell and Tissue Culture In Vitro. Plants, 13(2), 327. https://doi.org/10.3390/plants13020327

Pati, P. K., Rath, S. P., Sharma, M., Sood, A., & Ahuja, P. S. (2006). In Vitro Propagation of Rose—A Review. Biotechnology Advances, 24(1), 94–114. https://doi.org/10.1016/j.biotechadv.2005.07.001

Poerba, Y. S., Imelda, M., Wulansari, A., & Martanti, D. (2016). Induksi Mutasi Kultur In Vitro Amorphophallus muelleri Blume dengan Irradiasi Gamma. Jurnal Teknologi Lingkungan, 10(3), 355. https://doi.org/10.29122/jtl.v10i3.1482

Purnomo, M. H., Rizal, R., & Sundari, S. (2022). Status Keberlanjutan Usahatani Agroforestry Tanaman Porang pada Lembaga Masyarakat Desa Hutan Kemuning Asri di Gombengsari Kalipuro. Jurnal Agrinika : Jurnal Agroteknologi Dan Agribisnis, 6(1), 45. https://doi.org/10.30737/agrinika.v6i1.2143

Putri, A. D. P. L., Wiendi, N. M. A., & Santosa, E. (2023). Proliferation of Porang (Amorphophallus muelleri Blume) from Bulbils and Leaf Cutting Treated by NAA and BA. Jurnal Agronomi Indonesia, 51(3), 299–311. https://doi.org/10.24831/ija.v51i3.48958

Rezanejad, F., Abarian, M., & Abdirad, S. (2021). Shoot and Root Induction and Growth of Single Nodes of Rosa damascena in Different Culture Media. Journal of Plant Physiology and Breeding, 11(2), 97–108. https://doi.org/10.22034/JPPB.2021.14576

Royani, J. I., Chotimah, S., Utami, R. N., Fatma, W. S., Susiyanti, & Fatmawaty, A. P. (2021). Effect of Benzilaminopurine and Kinetin for shoot multiplication of Indigofera (Indigofera zollingeriana Miq.) by in vitro culture. IOP Conference Series: Earth and Environmental Science, 637(1), 012053. https://doi.org/10.1088/1755-1315/637/1/012053

Santosa, E. (2015). Pengembangan Tanaman Iles-Iles Tumpangsari untuk Kesejahteraan Petani dan Kemandirian Industri Pangan Nasional. RISALAH KEBIJAKAN PERTANIAN DAN LINGKUNGAN: Rumusan Kajian Strategis Bidang Pertanian Dan Lingkungan, 1(2), 73. https://doi.org/10.20957/jkebijakan.v1i2.10288

Sari, R., & Suhartati. (2015). Tumbuhan Porang: Prospek Budidaya sebagai Salah Satu Sistem Agroforestry. Info Teknis EBONI, 12(2), 97–110. https://doi.org/10.20886/buleboni.5061

Sompornpailin, K., & Khunchuay, C. (2016). Synergistic Effects of BAP and Kinetin Media Additives on Regeneration of Vetiver Grass (Vetiveria zizanioides L. Nash). Australian Journal of Crop Science, 10(05), 726–731. https://doi.org/10.21475/ajcs.2016.10.05.p7439

Sripaoraya, S., Marchant, R., Power, J. B., & Davey, M. R. (2003). Plant Regeneration by Somatic Embryogenesis and Organogenesis in Commercial Pineapple (Ananas comosus L.). In Vitro Cellular & Developmental Biology - Plant, 39(5), 450–454. https://doi.org/10.1079/IVP2003445

Sukartono, Suwardji, Kusumo, B. H., Bakti, A. A., & Edwin. (2020). Penguatan Kapasitas Kelompok Tani Dalam Budidaya Porang Berbasis Pertanian Konservasi-Agroforestry Di Desa Sambi Elen, Lombok Utara. Jurnal SIAR ILMUWAN TANI, 1(2), 67–74. https://doi.org/10.29303/jsit.v1i2.19

Sumarwoto. (2005). Iles-iles (Amorphophallus muelleri Blume); Deskripsi dan Sifat-sifat Lainnya. Biodiversitas Journal of Biological Diversity, 6(3), 185–190. https://doi.org/10.13057/biodiv/d060310

Tajuddin, M., Santosa, E., Sopandie, D., & Lontoh, A. P. (2020). Characteristics of growth, flowering and corm yield of iles-iles (Amorphophallus muelleri) genotypes at third growing period. Biodiversitas Journal of Biological Diversity, 21(2). https://doi.org/10.13057/biodiv/d210219

Tilaar, W., Rantung, J., & Tulung, S. (2015). Induksi Tunas dari Nodul Krisan Kulo dalam Media Murashige dan Skoog yang Diberi Sitokinin. EUGENIA, 21(2). https://doi.org/10.35791/eug.21.2.2015.9713

Van Khiem, D., Xuan Huyen, P., Thanh Hang, N. T., & Phuong Hoang, N. T. (2022). In Vitro Regeneration and Acclimatization of Anthurium scherzerianum Schott Plants. Academia Journal of Biology, 44(3), 99–109. https://doi.org/10.15625/2615-9023/16548

Verbeke, P., Siboska, G. E., Clark, B. F. C., & Rattan, S. I. S. (2000). Kinetin Inhibits Protein Oxidation and Glycoxidation In Vitro. Biochemical and Biophysical Research Communications, 276(3), 1265–1270. https://doi.org/10.1006/bbrc.2000.3616

Wardani, N. E., Subaidah, W. A., & Muliasari, H. (2021). Ekstraksi dan Penetapan Kadar Glukomanan dari Umbi Porang (Amorphophallus muelleri Blume) Menggunakan Metode DNS. Jurnal Sains Dan Kesehatan, 3(3), 383–391. https://doi.org/10.25026/jsk.v3i3.574

Yadav, R., Yadav, N., & Kumar, S. (2016). An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 86(4), 921–929. https://doi.org/10.1007/s40011-015-0550-9

Author Biography

Rut Normasari, Universitas Klabat

Author Origin : Indonesia

Downloads

Download data is not yet available.

How to Cite

Normasari, R. (2025). In Vitro Growth Response of Porang (Amorphophallus muelleri Blume) Bulbil to Kinetin Concentration. Jurnal Biologi Tropis, 25(2), 2004–2011. https://doi.org/10.29303/jbt.v25i2.9050

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.