Potential of Antimicrobial Peptides (AMPs) Extracted from Black Soldier Fly Larvae Against Vibrio parahaemolyticus In Vitro
DOI:
10.29303/jbt.v25i4a.9655Published:
2025-11-26Downloads
Abstract
Whiteleg shrimp (Litopenaeus vannamei) is one of the most widely farmed shrimp species; however, its cultivation is frequently threatened by vibriosis caused by Vibrio parahaemolyticus. To date, the potential of antimicrobial peptides (AMPs) derived from Black Soldier Fly (BSF) larvae as natural antimicrobial agents has not been reported. This study aimed to evaluate the effectiveness of BSF larval AMPs against V. parahaemolyticus, the causative agent of vibriosis in L. vannamei. Data were analyzed using a quantitative descriptive approach. The results showed that BSF larval AMPs contained protein levels ranging from 5.2–12.03% in the first trial and 9.19–10.82% in the second trial, while the water content varied between 4.77% and 77.89%. In vitro testing demonstrated that BSF larval AMPs inhibited V. parahaemolyticus growth with a 9 mm inhibition zone in all treatments. Further in vivo studies and optimization are necessary to develop BSF larval AMPs as sustainable, eco-friendly antimicrobial agents.
Keywords:
Antimicrobial peptides (AMPs); SDS Page; Vanname shrimp; Vibrio parahaemolyticusReferences
Abu, G. O., & Egenonu, C. (2008). The current pollution status of the new Calabar River in the Niger Delta region of Southern Nigeria: A survey of antibiogram profiles of its bacterial isolates. African Journal of Environmental Science and Technology, 2(6), 134-141.
Ahmed, I., Jan, K., Fatma, S., & Dawood, M. A. (2022). Muscle proximate composition of various food fish species and their nutritional significance: A review. Journal of Animal Physiology and Animal Nutrition, 106(3), 690-719. DOI: 10.1111/jpn.13711
Ambrosio, R. L., Rosselló, C. A., Casares, D., Palmieri, G., Anastasio, A., & Escribá, P. V. (2022). The antimicrobial peptide 1018-K6 interacts distinctly with eukaryotic and bacterial membranes, the basis of its specificity and bactericidal activity. International journal of molecular sciences, 23(20), 12392. DOI: https://doi.org/10.3390/ijms232012392
Amirna, O., Iba, R., & Rahman, A. (2013). Pemberian Silase Ikan Gabus pada Pakan Buatan bagi Pertumbuhan dan Kelangsungan Hidup Udang Vaname (Litopenaeus vannamei) pada Stadia Post Larva. Jurnal Mina laut Indonesia, 1(01), 93-103.
Anggraini, A. dan Yunianta. (2015). Pengaruh Suhu dan Lama Hidrolisis Enzim Papain terhadap Sifat Kimia, Fisik, dan Organoleptik Sari Edamame. Jurnal Pangan dan Agroindustri. 3(3): 1015-1025
Austin, B. (2010). Vibrios as causal agents of zoonoses. Veterinary microbiology, 140(3-4), 310-317. DOI: 10.1016/j.vetmic.2009.03.015ff.
Cahyono, B., Diah Khoirul Huda, M., & Limantara, L. (2011). Pengaruh proses pengeringan rimpang temulawak (Curcuma xanthorriza roxb) terhadap kandungan dan komposisi kurkuminoid. Reaktor. 13 (3): 165-171.DOI:https://doi.org/10.14710/reaktor.13.3.165-171
Ceccarelli, D., Hasan, N. A., Huq, A., & Colwell, R. R. (2013). Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors. Frontiers in cellular and infection microbiology, 3, 97. DOI: https://doi.org/10.3389/fcimb.2013.00097
Čičková H, Newton GL, Lacy RC, Kozánek M. (2015). The use of fly larvae for organic waste treatment. Waste Manag. 35:68-80. DOI: http://dx.doi.org/10.1016/j.wasman.2014.09.026
Dunn, M. J. (Ed.). (2014). Gel electrophoresis of proteins. Elsevier.
Haslaniza, H. (2010). The effects of enzyme concentration, temperature and incubation time on nitrogen content and degree of hydrolysis of protein precipitate from cockle (Anadara granosa) meat wash water. International Food Research Journal. 17: 147- 152
Hidayat, T. (2005). Pembuatan hidrolisat protein dari ikan selar kuning (Caranx leptolepis) dengan menggunakan enzim papain. Skripsi. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor.
Jannah, M., Junaidi, M., Setyowati, D. N. & Azhar, F. (2018). Pengaruh Pemberian Lactobacillus sp. Dengan Dosis Yang Berbeda Terhadap Sistem Imun Udang Vaname (Litopenaeus vannamei) yang Diinfeksi Bakteri Vibrio parahaemolyticus. Jurnal Kelautan. 11(2): 140-150.
Khusuma A., Safitri, Y., Yuniarni, A., & Rizki, K. (2019). Uji Teknik Difusi menggunakan Kertas Saring Media Tampung Antibiotik dengan Escherichia Coli sebagai Bakteri Uji. Jurnal Kesehatan Prima. 13(2): 151- 155. https://doi.org/10.32807/jkp.v13i2.257
Leni, G., Del Vecchio, L., Dellapina, C., Moliterni, V. M. C., Caligiani, A., & Cirlini, M. (2024). Black soldier fly larvae grown on hemp fiber: nutritional composition and production of potential bioactive peptides. Macromol, 4(1), 135-149. DOI: https://doi.org/10.3390/macromol4010007
Li, M., Cha, D. J., Lai, Y., Villaruz, A. E., Sturdevant, D. E., & Otto, M. (2007). The antimicrobial peptide‐sensing system aps of Staphylococcus aureus. Molecular microbiology, 66(5), 1136-1147. doi:10.1111/j.1365-2958.2007.05986.x.
Miron, L. T., Postma, R. P., Bosch, G., & Eppink, M. H. (2019). Preliminary evaluation of aqueous protein extraction from black soldier fly larvae (Hermetia illucens L.). Industrial Biotechnology, 15(6), 365-369.
O’Kelly, B. C. and Sivakumar, V. 2014. Water content determinations for peat and other organic soils using the oven-drying method. Drying Technology; An International Journal. 32(13): 1-31
Pratama, P. N., & Prayitno, S. B. (2014). Pemanfaatan Ekstrak Daun Binahong (Anredera Cordifolia) Untuk Penanggulangan Penyakit Bakteri (Vibrio Harveyi) Pada Udang Windu. Journal of Aquaculture Management and Technology, 3(4), 281-288.
Prescott, L.M. 2005. Microbiology. 6th -Ed. McGraw-Hill, New York.
Purwani, E., Hapsari, S. W. (2011). Pengaruh Ekstrak Jahe (Zingiber officinale) terhadap Penghambatan Mikroba Perusak pada Ikan Nila (Oreochromis niloticus). Jurnal Kesehatan. 4(1): 80-91
Sudarmadji, S., Haryono, B., & Suhardi. (1996). Analisa Bahan Makanan dan Pertanian. Yogyakarta: Liberty Yogyakarta
Surahman, N., Surati, Ryan, R. (2017). Aktivitas Enzim Bromelin Terhadap Peningkatan Protein Tepung Ampas Kelapa. IAIN Ambon.
Tooragzadeh, O., Piri, H., Naserin, A., & Chari, M. M. (2023). Investigation the effect of biochar and irrigation water salinity on yield indicators and protein percentage of quinoa in deficit irrigation conditions. Iranian Journal of Irrigation & Drainage, 17(4), 597-610.
Torres, M.D.T., Sothiselvam, S., Lu, T.K., & Fuente-Nunez, C.d.l. (2019). Peptide design principles for antimicrobial applications. J. Mol. Biol. 431(18): 3547-3567
Wardhana, A. H. (2016). Black soldier fly (Hermetia illucens) sebagai sumber protein alternatif untuk pakan ternak. Wartazoa, 26(2), 69-78.
Winarno, F.G. (2004). Kimia Pangan dan Gizi. Jakarta: Gramedia Pustaka Utama
Winarno, F.G. (2008). Kimia Pangan dan Gizi. PT. Gramedia. Jakarta.
License
Copyright (c) 2025 Riskia Khanifa, Aida Himmatul Ulya, Ahmad Edi Darmawan, Anis H. Mahsunah, Wahyu Aji Mahardhika
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
